The methylotrophic yeast Pichia pastoris is a highly successful system for production of a variety of heterologous proteins due to its unique features/abilities for effective protein expression, and tremendous efforts...The methylotrophic yeast Pichia pastoris is a highly successful system for production of a variety of heterologous proteins due to its unique features/abilities for effective protein expression, and tremendous efforts have been made to increase heterologous protein productivity by P. pastoris in recent years. When new engineered yeast strains are constructed and are ready to use tot industrial protein production, process control and optimization techniques should be applied to improve the fermentation performance in the following aspects： （1） increase recombinant cell concentrations in fermentor to high density during growth phase; （2） effectively induce heterologous proteins by enhancing/stabilizing titers or concentrations of the proteins during induction phase; （3） decrease operation costs by relieving the working loads of heat-exchange and oxygen supply. This article reviews and discusses the key and commonly used techniques in heterologous protein production by P. pastoris, with the focus on optimizations of fermentation media and basic operation conditions, development of optimal glycerol feeding strategies for achieving high density cultivation of P. pastoris and effective heterologous protein induction methods by regulating specific growth rate, methanol concentration, temperatures, mixture ratio of multi-carbon substrates, etc. Metabolic analysis for recombinant protein production by P. pastoris is also introduced to interpret the mechanism of sub-optimal heterologous protein production and to explore further optimal expression methods.展开更多
Chemical modification of MWCNTs via oxidation followed by side wall functionalization using polyethylene glycol （PEG） and octadecylamine （ODA）, separately, was studied. Different characterization techniques such a...Chemical modification of MWCNTs via oxidation followed by side wall functionalization using polyethylene glycol （PEG） and octadecylamine （ODA）, separately, was studied. Different characterization techniques such as FTIR spectrometery, thermogravimetric analysis （TGA）, scanning electron microscopy （SEM）, X-ray diffraction （XRD）, and solubility in different solvents were performed for the oxidized MWCNTs, MWCNTs-PEG and MWCNTs-ODA. The characterization techniques proved the presence of the functional groups on the MWCNTs surface. Thermal gravimetric analysis revealed that nearly 16% （by weight） of the MWCNTs were functionalized with PEG and 39% （by weight） was functionalized with ODA.展开更多
Objective： The aim of our study was to evaluate the toxicity and efficacy of induction chemotherapy （ICT） followed by three-dimensional conformal radiotherapy （3D CRT） and concurrent weekly paclitaxel on unresect...Objective： The aim of our study was to evaluate the toxicity and efficacy of induction chemotherapy （ICT） followed by three-dimensional conformal radiotherapy （3D CRT） and concurrent weekly paclitaxel on unresectable non-small cell lung cancer （NSCLC）. Methods： Stage III NSCLC patients with favorable conditions were treated with 2 to 4 cycles of carboplatin （AUC = 5-6, dl） combined with paclitaxel （175 mg/m〈 dl）, then followed by weekly paclitaxel （40 mg/m2） and concurrent 3D CRT within 3-4 weeks. The prescription dose was given as high as possible under the condition that V20 〈 31% and spinal cord dose 〈 50 Gy. Results： Thirty-one patients were enrolled. ICT was well tolerated. During the concurrent chemoradiotherapy, the treatment of 3 patients was ended ahead of the schedule because of severe pulmonary and heart toxicities; the treatment of 2 patients was delayed for 7 and 12 days because of fatigue. Myelosuppression was mild （16/31）： all were grade 1-2 except 1 was grade 3. Lymphocytopenia was more obvious （29/31, grade 3 in 21）. Three patients developed grade 3 radiation-induced esophagitis, and 2 developed grades 3-4 radiation-induced pneumonitis. Two developed grade 3 esophageal stricture. No grades 3-4 pulmonary fibrosis was observed. The overall response rate was 74.1%. The 1-, 2-, 3-year overall survival rates were 74.2%, 41.9%, and 34.6%, respectively, with the median survival time of 18.5 months. The 1-, 2-, 3-year local progression-freely survival rates were 64.5%, 32.3%, and 20.5%, respectively, with the median local progression-freely survival time of 14.3 months. Conclusion： The program of ICT followed by weekly paclitaxel and 3D CRT is accomplished in most of the favorable stage III NSCLC patients. The toxicity is tolerable, and the response rate is inspiriting.展开更多
The key project'Nano-scale catalyst and related process research and development activity for high-efficiency synthesis of mixed lower alcohols from syngas'undertaken by the Shanghai Senior Research Institute ...The key project'Nano-scale catalyst and related process research and development activity for high-efficiency synthesis of mixed lower alcohols from syngas'undertaken by the Shanghai Senior Research Institute of CAS has passed the acceptance tests by the expert group organized by the Shanghai Science and Technology Commission.展开更多
On September 14,2015 the 10 kt/a-class commercialized test project relating to the technology for methanolto-butene production with co-production of propylene(hereafter abbreviated as CMTX)has passed the scientific ac...On September 14,2015 the 10 kt/a-class commercialized test project relating to the technology for methanolto-butene production with co-production of propylene(hereafter abbreviated as CMTX)has passed the scientific achievement appraisal organized by the China Petroleum and Chemical Industries Federation(CPCIF).展开更多
The project ＂SolMethCO2＂ deals with the options of an effective methanol synthesis from atmospheric or industrial CO2 sources by implementing solar energy. First part of the projects is a wide-range survey of the ma...The project ＂SolMethCO2＂ deals with the options of an effective methanol synthesis from atmospheric or industrial CO2 sources by implementing solar energy. First part of the projects is a wide-range survey of the many different processes and sub-processes that may be involved in methanol production and of the possibilities how to make these processes available for solarization. The different fields of research were CO2 capturing, Hg/syngas-synthesis, biotechnological techniques for methanol synthesis, photocatalytical approaches and solar reactor.展开更多
基金Supported by the Key Agricultral Technology Program of Shanghai Science & Technology Committee(073919108)MajorState Basic Research Development Program of China(2007CB714303)
文摘The methylotrophic yeast Pichia pastoris is a highly successful system for production of a variety of heterologous proteins due to its unique features/abilities for effective protein expression, and tremendous efforts have been made to increase heterologous protein productivity by P. pastoris in recent years. When new engineered yeast strains are constructed and are ready to use tot industrial protein production, process control and optimization techniques should be applied to improve the fermentation performance in the following aspects： （1） increase recombinant cell concentrations in fermentor to high density during growth phase; （2） effectively induce heterologous proteins by enhancing/stabilizing titers or concentrations of the proteins during induction phase; （3） decrease operation costs by relieving the working loads of heat-exchange and oxygen supply. This article reviews and discusses the key and commonly used techniques in heterologous protein production by P. pastoris, with the focus on optimizations of fermentation media and basic operation conditions, development of optimal glycerol feeding strategies for achieving high density cultivation of P. pastoris and effective heterologous protein induction methods by regulating specific growth rate, methanol concentration, temperatures, mixture ratio of multi-carbon substrates, etc. Metabolic analysis for recombinant protein production by P. pastoris is also introduced to interpret the mechanism of sub-optimal heterologous protein production and to explore further optimal expression methods.
文摘Chemical modification of MWCNTs via oxidation followed by side wall functionalization using polyethylene glycol （PEG） and octadecylamine （ODA）, separately, was studied. Different characterization techniques such as FTIR spectrometery, thermogravimetric analysis （TGA）, scanning electron microscopy （SEM）, X-ray diffraction （XRD）, and solubility in different solvents were performed for the oxidized MWCNTs, MWCNTs-PEG and MWCNTs-ODA. The characterization techniques proved the presence of the functional groups on the MWCNTs surface. Thermal gravimetric analysis revealed that nearly 16% （by weight） of the MWCNTs were functionalized with PEG and 39% （by weight） was functionalized with ODA.
文摘Objective： The aim of our study was to evaluate the toxicity and efficacy of induction chemotherapy （ICT） followed by three-dimensional conformal radiotherapy （3D CRT） and concurrent weekly paclitaxel on unresectable non-small cell lung cancer （NSCLC）. Methods： Stage III NSCLC patients with favorable conditions were treated with 2 to 4 cycles of carboplatin （AUC = 5-6, dl） combined with paclitaxel （175 mg/m〈 dl）, then followed by weekly paclitaxel （40 mg/m2） and concurrent 3D CRT within 3-4 weeks. The prescription dose was given as high as possible under the condition that V20 〈 31% and spinal cord dose 〈 50 Gy. Results： Thirty-one patients were enrolled. ICT was well tolerated. During the concurrent chemoradiotherapy, the treatment of 3 patients was ended ahead of the schedule because of severe pulmonary and heart toxicities; the treatment of 2 patients was delayed for 7 and 12 days because of fatigue. Myelosuppression was mild （16/31）： all were grade 1-2 except 1 was grade 3. Lymphocytopenia was more obvious （29/31, grade 3 in 21）. Three patients developed grade 3 radiation-induced esophagitis, and 2 developed grades 3-4 radiation-induced pneumonitis. Two developed grade 3 esophageal stricture. No grades 3-4 pulmonary fibrosis was observed. The overall response rate was 74.1%. The 1-, 2-, 3-year overall survival rates were 74.2%, 41.9%, and 34.6%, respectively, with the median survival time of 18.5 months. The 1-, 2-, 3-year local progression-freely survival rates were 64.5%, 32.3%, and 20.5%, respectively, with the median local progression-freely survival time of 14.3 months. Conclusion： The program of ICT followed by weekly paclitaxel and 3D CRT is accomplished in most of the favorable stage III NSCLC patients. The toxicity is tolerable, and the response rate is inspiriting.
文摘The key project'Nano-scale catalyst and related process research and development activity for high-efficiency synthesis of mixed lower alcohols from syngas'undertaken by the Shanghai Senior Research Institute of CAS has passed the acceptance tests by the expert group organized by the Shanghai Science and Technology Commission.
文摘On September 14,2015 the 10 kt/a-class commercialized test project relating to the technology for methanolto-butene production with co-production of propylene(hereafter abbreviated as CMTX)has passed the scientific achievement appraisal organized by the China Petroleum and Chemical Industries Federation(CPCIF).
文摘The project ＂SolMethCO2＂ deals with the options of an effective methanol synthesis from atmospheric or industrial CO2 sources by implementing solar energy. First part of the projects is a wide-range survey of the many different processes and sub-processes that may be involved in methanol production and of the possibilities how to make these processes available for solarization. The different fields of research were CO2 capturing, Hg/syngas-synthesis, biotechnological techniques for methanol synthesis, photocatalytical approaches and solar reactor.
