Gold nanoparticles, prepared by the Frens method, have been electrostatically assemblied onto mica substrates treated with different concentration of MnCl\-2 solution. The assemblies have been investigated by means of...Gold nanoparticles, prepared by the Frens method, have been electrostatically assemblied onto mica substrates treated with different concentration of MnCl\-2 solution. The assemblies have been investigated by means of atomic force microscopy(AFM). The immersing time of bare mica in Mn\+\{2+\} solution and the concentration of Mn\+\{2+\} solution both have significant effect on the assembled process of gold nanoparticulate monolayer. The coverage of gold particles shows periodical evolution on the immersing time of mica in MnCl\-2 solution. The higher is the concentration of MnCl\-2, the shorter is the time to attain maximum coverage. An ion\|exchanged and layer\|dissociated mechanism is put forth to explain the experimental results above.展开更多
纳米金颗粒具有高的消光系数和良好的表面等离子体共振特性,其等离子体共振特性受纳米金颗粒的尺寸和周围环境等因素的影响.本文基于半导体纳米晶电化学发光信号对金纳米颗粒的距离依赖性制备了DNA电化学发光传感器.首先利用循环伏安法(...纳米金颗粒具有高的消光系数和良好的表面等离子体共振特性,其等离子体共振特性受纳米金颗粒的尺寸和周围环境等因素的影响.本文基于半导体纳米晶电化学发光信号对金纳米颗粒的距离依赖性制备了DNA电化学发光传感器.首先利用循环伏安法(CV)在玻碳电极(GCE)表面原位沉积金纳米颗粒(Au NPs),巯基丙酸包裹的Cd S量子点(QDs)与氨基修饰的双链DNA(ds DNA)通过酰胺键缩合,形成量子点修饰的双链DNA(QDs-ds DNA).最后将QDs-ds DNA通过ds DNA另一端的巯基组装到纳米金表面,得到Cd S QDs-DNA/Au NPs/GCE电化学发光传感器.在优化电极表面QDs-ds DNA密度、金纳米颗粒沉积方法等实验条件的基础上,对不同传感器的表面性质进行了表征,如形貌和电化学阻抗等.进一步通过控制纳米金和Cd S QDs之间的DNA研究了纳米金对Cd S QDs发光信号的影响作用.结果显示DNA链的长度和类型对发光信号有着重要的影响.最后将此传感器用于环境污染物的DNA损伤检测,显示出很好的灵敏响应.展开更多
基金Merit-funded Science and Technology Project for Returned Oversea Scholars from Ministry of Human and Social Security of Shanxi provinceNatural Science Foundation for Young Scientists of Shanxi province(No.2011011020-2)Shanxi Province Foundation for Returness(No.2008062)
文摘Gold nanoparticles, prepared by the Frens method, have been electrostatically assemblied onto mica substrates treated with different concentration of MnCl\-2 solution. The assemblies have been investigated by means of atomic force microscopy(AFM). The immersing time of bare mica in Mn\+\{2+\} solution and the concentration of Mn\+\{2+\} solution both have significant effect on the assembled process of gold nanoparticulate monolayer. The coverage of gold particles shows periodical evolution on the immersing time of mica in MnCl\-2 solution. The higher is the concentration of MnCl\-2, the shorter is the time to attain maximum coverage. An ion\|exchanged and layer\|dissociated mechanism is put forth to explain the experimental results above.
文摘纳米金颗粒具有高的消光系数和良好的表面等离子体共振特性,其等离子体共振特性受纳米金颗粒的尺寸和周围环境等因素的影响.本文基于半导体纳米晶电化学发光信号对金纳米颗粒的距离依赖性制备了DNA电化学发光传感器.首先利用循环伏安法(CV)在玻碳电极(GCE)表面原位沉积金纳米颗粒(Au NPs),巯基丙酸包裹的Cd S量子点(QDs)与氨基修饰的双链DNA(ds DNA)通过酰胺键缩合,形成量子点修饰的双链DNA(QDs-ds DNA).最后将QDs-ds DNA通过ds DNA另一端的巯基组装到纳米金表面,得到Cd S QDs-DNA/Au NPs/GCE电化学发光传感器.在优化电极表面QDs-ds DNA密度、金纳米颗粒沉积方法等实验条件的基础上,对不同传感器的表面性质进行了表征,如形貌和电化学阻抗等.进一步通过控制纳米金和Cd S QDs之间的DNA研究了纳米金对Cd S QDs发光信号的影响作用.结果显示DNA链的长度和类型对发光信号有着重要的影响.最后将此传感器用于环境污染物的DNA损伤检测,显示出很好的灵敏响应.
