本研究采用AB-8大孔吸附树脂、半制备高效液相色谱对金露梅枝叶中槲皮素-7-O-β-D-葡糖苷酸提取分离,并通过13 C NMR鉴定其化学结构,最后采用以4-硝基酚-α-D-吡喃葡萄糖苷(pNPG)为底物的酶抑制剂筛选模型检测其对α-葡萄糖苷酶的抑制...本研究采用AB-8大孔吸附树脂、半制备高效液相色谱对金露梅枝叶中槲皮素-7-O-β-D-葡糖苷酸提取分离,并通过13 C NMR鉴定其化学结构,最后采用以4-硝基酚-α-D-吡喃葡萄糖苷(pNPG)为底物的酶抑制剂筛选模型检测其对α-葡萄糖苷酶的抑制活性和类型。结果显示:槲皮素-7-O-β-D-葡糖苷酸分布在20%乙醇大孔吸附树脂(AB-8)洗脱液中,采用半制备高效液相色谱和高效液相色谱分离纯化得槲皮素-7-O-β-D-葡糖苷酸2481.8 mg,纯度为97.95%。活性检测结果表明,槲皮素-7-O-β-D-葡糖苷酸对α-葡萄糖苷酶的最大抑制率为94.67%,IC 50为0.259 mmol/L,抑制率显著高于阳性对照药物阿卡波糖,且抑制剂类型为竞争性抑制。展开更多
The techniques of cutting propagation of Potentilla fruticosa was studied.The tender stem cuttings and the woody stem cuttings were treated by different concentrations of BBA(0 mg·kg-1,10 mg·kg-1,50 mg·...The techniques of cutting propagation of Potentilla fruticosa was studied.The tender stem cuttings and the woody stem cuttings were treated by different concentrations of BBA(0 mg·kg-1,10 mg·kg-1,50 mg·kg-1,100 mg·kg-1,200 mg·kg-1,500 mg·kg-1).The results indicated that the rooting percentage of tender stem cuttings at the concentration of 10 mg ·kg-1BBA was the highest(88.3%),and that of woody stem cuttings treated by the concentration of 50 mg·kg-1BBA was the highest(66.67%).It also indicated that the rooting percentages of the tender stem cuttings were better than those of the woody stem cuttings with same treatment.展开更多
文摘The techniques of cutting propagation of Potentilla fruticosa was studied.The tender stem cuttings and the woody stem cuttings were treated by different concentrations of BBA(0 mg·kg-1,10 mg·kg-1,50 mg·kg-1,100 mg·kg-1,200 mg·kg-1,500 mg·kg-1).The results indicated that the rooting percentage of tender stem cuttings at the concentration of 10 mg ·kg-1BBA was the highest(88.3%),and that of woody stem cuttings treated by the concentration of 50 mg·kg-1BBA was the highest(66.67%).It also indicated that the rooting percentages of the tender stem cuttings were better than those of the woody stem cuttings with same treatment.
