采用硅胶柱色谱和半制备反相高效液相色谱分离方法,对南海海洋放线菌Streptomyces lusitanus SCSIOLR32的次级代谢产物进行了研究,分离得到四个酰胺类化合物,经MS1、H和13 C NMR波谱分析鉴定为二甲基甲苯2,4-二氨基甲酸甲酯(1),甲苯2,4...采用硅胶柱色谱和半制备反相高效液相色谱分离方法,对南海海洋放线菌Streptomyces lusitanus SCSIOLR32的次级代谢产物进行了研究,分离得到四个酰胺类化合物,经MS1、H和13 C NMR波谱分析鉴定为二甲基甲苯2,4-二氨基甲酸甲酯(1),甲苯2,4-二氨基甲酸乙酯(2),甲苯2,6-二氨基甲酸甲酯(3)和甲苯2,6-二氨基甲酸乙酯(4)。运用X-单晶衍射确定了1的结构式。其中化合物2和4是首次从自然界中分离得到。采用16S分子生物学方法鉴定该菌株为链霉菌属放线菌。展开更多
分离、鉴定赤水丹霞地区土壤来源的抗临床耐药菌活性的链霉菌。采集赤水丹霞山区不同海拔的土样并利用多种培养基分离、纯化菌株。热酚法提取菌株基因组DNA,PCR扩增16S r RNA基因并测序后在线比对以确定其系统分类学地位。利用多种测试...分离、鉴定赤水丹霞地区土壤来源的抗临床耐药菌活性的链霉菌。采集赤水丹霞山区不同海拔的土样并利用多种培养基分离、纯化菌株。热酚法提取菌株基因组DNA,PCR扩增16S r RNA基因并测序后在线比对以确定其系统分类学地位。利用多种测试菌测试抗菌活性,通过HPLC和LC/MS分析菌株发酵抽提物并与数据库比较。分离得到菌株600株,其中59株链霉菌中有41株具有1种以上的抗测试菌活性。Streptomycetes sp.CSDX001具有抗耐药耐甲氧西林金黄色葡萄球菌、抗耐万古霉素金黄色葡萄球菌、抗多药耐药鲍曼不动杆菌、抗枯草芽孢杆菌、抗藤黄微球菌、抗白色念球菌的活性,具有抗多种微生物的活性和产生多种新结构次级代谢产物潜力,可能是抗微生物新天然活性产物的重要来源。展开更多
Streptomyces tenebrarius H6 produces a complex of aminoglycoside antibiotics,such as apramycin,tobramycin and kanamycin B etc. To study the apramycin biosynthetic genes the genomic library from the Streptomyces tenebr...Streptomyces tenebrarius H6 produces a complex of aminoglycoside antibiotics,such as apramycin,tobramycin and kanamycin B etc. To study the apramycin biosynthetic genes the genomic library from the Streptomyces tenebrarius H6 was established using E.coli/Streptomyces shuttle vector pKC505 by in vitro packing.The probability of finding a specific gene from the library composed of 3 000 colonies was over 99 9%. According to the highly conserved sequence of the genes involved in 6\|deoxyhexose biosynthesis,primers were designed and 0 6kb fragment homologous to strE gene was obtained by PCR.30 positive clones were found from the genomic library of \%S.tenebrarius\% H6 with the 0 6kb fragment as a probe.Overlapped regions were localized by Southern hybridization and putative sugar related biosynthetic gene cluster was mapped by restriction enzyme digestions.An ORF of dTDP\|glucose\|4,6\|dehydratase gene consisted of 1,132bp,designated as apr E,was obtained and submitted to GenBank under the accession number of AF306787.A DNA sequence highly homologous to str L coding dTDP\|4\|dehydrorhamnose reductase was found linked with apr E gene.展开更多
文摘采用硅胶柱色谱和半制备反相高效液相色谱分离方法,对南海海洋放线菌Streptomyces lusitanus SCSIOLR32的次级代谢产物进行了研究,分离得到四个酰胺类化合物,经MS1、H和13 C NMR波谱分析鉴定为二甲基甲苯2,4-二氨基甲酸甲酯(1),甲苯2,4-二氨基甲酸乙酯(2),甲苯2,6-二氨基甲酸甲酯(3)和甲苯2,6-二氨基甲酸乙酯(4)。运用X-单晶衍射确定了1的结构式。其中化合物2和4是首次从自然界中分离得到。采用16S分子生物学方法鉴定该菌株为链霉菌属放线菌。
文摘分离、鉴定赤水丹霞地区土壤来源的抗临床耐药菌活性的链霉菌。采集赤水丹霞山区不同海拔的土样并利用多种培养基分离、纯化菌株。热酚法提取菌株基因组DNA,PCR扩增16S r RNA基因并测序后在线比对以确定其系统分类学地位。利用多种测试菌测试抗菌活性,通过HPLC和LC/MS分析菌株发酵抽提物并与数据库比较。分离得到菌株600株,其中59株链霉菌中有41株具有1种以上的抗测试菌活性。Streptomycetes sp.CSDX001具有抗耐药耐甲氧西林金黄色葡萄球菌、抗耐万古霉素金黄色葡萄球菌、抗多药耐药鲍曼不动杆菌、抗枯草芽孢杆菌、抗藤黄微球菌、抗白色念球菌的活性,具有抗多种微生物的活性和产生多种新结构次级代谢产物潜力,可能是抗微生物新天然活性产物的重要来源。
文摘Streptomyces tenebrarius H6 produces a complex of aminoglycoside antibiotics,such as apramycin,tobramycin and kanamycin B etc. To study the apramycin biosynthetic genes the genomic library from the Streptomyces tenebrarius H6 was established using E.coli/Streptomyces shuttle vector pKC505 by in vitro packing.The probability of finding a specific gene from the library composed of 3 000 colonies was over 99 9%. According to the highly conserved sequence of the genes involved in 6\|deoxyhexose biosynthesis,primers were designed and 0 6kb fragment homologous to strE gene was obtained by PCR.30 positive clones were found from the genomic library of \%S.tenebrarius\% H6 with the 0 6kb fragment as a probe.Overlapped regions were localized by Southern hybridization and putative sugar related biosynthetic gene cluster was mapped by restriction enzyme digestions.An ORF of dTDP\|glucose\|4,6\|dehydratase gene consisted of 1,132bp,designated as apr E,was obtained and submitted to GenBank under the accession number of AF306787.A DNA sequence highly homologous to str L coding dTDP\|4\|dehydrorhamnose reductase was found linked with apr E gene.
