In order to explore a breeding method for mycoplasmal pneumonia-negative swine population, the purifying effects of combination therapy, SEW and three-point breeding and management system on Mycoplasma hyopneumoniae w...In order to explore a breeding method for mycoplasmal pneumonia-negative swine population, the purifying effects of combination therapy, SEW and three-point breeding and management system on Mycoplasma hyopneumoniae were investigated in this study. After the processes of screening pregnant sows, programmed therapy for sows, SEW, three-point breeding and management in the barrier isolation system and programmed therapy for piglets, the newly-bred piglets were monitored as long as four months by serum antibody detection of Mycoplasma hyopneumoniae and fluorescent quantitative PCR of nose swabs. The results showed that when the newlybred five batches of piglets were older than 35 d, they were all negative by serum antibody detection and nose swab antigen detection. Therefore, the combination therapy, SEW and three-point production and management system can effectively purify Mycoplasma hyopneumoniae, providing theoretical basis and clinical experience for the control and purification of domestic mycoplasmal pneumonia.展开更多
Objective:To evaluate the effect and profitability of using the quantitative trait loci (QTL)-linked direct marker (DR marker) in gene-assisted selection (GAS). Methods: Three populations (100, 200, or 300 sows plus 1...Objective:To evaluate the effect and profitability of using the quantitative trait loci (QTL)-linked direct marker (DR marker) in gene-assisted selection (GAS). Methods: Three populations (100, 200, or 300 sows plus 10 boars within each group) with segregating QTL were simulated stochastically. Five economic traits were investigated, including number of born alive (NBA), average daily gain to 100 kg body weight (ADG), feed conversion ratio (FCR), back fat at 100 kg body weight (BF) and intramuscular fat (IMF). Selection was based on the estimated breeding value (EBV) of each trait. The starting frequencies of the QTL's favorable allele were 0.1, 0.3 and 0.5, respectively. The economic return was calculated by gene flow method. Results: The selection efficiency was higher than 100% when DR markers were used in GAS for 5 traits. The selection efficiency for NBA was the highest, and the lowest was for ADG whose QTL had the lowest variance. The mixed model applied DR markers and obtained higher extra genetic gain and extra economic returns. We also found that the lower the frequency of the favorable allele of the QTL, the higher the extra return obtained. Conclusion: GAS is an effective selection scheme to increase the genetic gain and the eco- nomic returns in pig breeding.展开更多
Marsupenaeusjaponicus Bate is one of the most valuable cultured shrimp species in China and outdoor earthen pond farming is the most common method of culturing this organism. The need to increase soil usage efficiency...Marsupenaeusjaponicus Bate is one of the most valuable cultured shrimp species in China and outdoor earthen pond farming is the most common method of culturing this organism. The need to increase soil usage efficiency in aquaculture has been recognized and a great deal of research effort has been directed toward development of super-intensive farming systems. However, current research and development in this field is largely devoted to Litopenaeus vannamei Boone, while to M.japonicus Bate it has been neglected. In this study, a layered fanning system was designed and a 66-day study was conducted in M.japonicus Bate culture. The system comprised bracket and sand layers that divided a shrimp tank filled to a depth of 1.2 m into four water layers. Conventional tank culture （unlayered） was used as a control. The results show that survival rate, feed conversion efficiency and production of M.japonicus Bate in the layered farming system were 68%, 18%, and 0.59 kg/m^2, respectively, all of which are significantly higher than in the unlayered fanning system （P〈0.01）. These findings confirmed the possibility of using a layered system to culture M. japonicus Bate.展开更多
Influenced by animal welfare requirements and expensive labor cost, producers tend to feed layers in non-cage system such as floor rearing. With the development of commercial lines of dwarf layers, the property of dwa...Influenced by animal welfare requirements and expensive labor cost, producers tend to feed layers in non-cage system such as floor rearing. With the development of commercial lines of dwarf layers, the property of dwarf layers＇ natural mating is drawing more and more attention. In this study, the authors hybridized Nongda Ill dwarf layers with White Leghorn chickens in floor-rearing system to research whether dwarf layers had defect in natural mating. Two steps of experiments were performed. The authors detected the fertilization rate of collected eggs, observed chickens＇ behavior in step I when a hen from other group was suddenly put into and recorded copulation number of every group in step II from 7：30 to 19：30. The results indicated that drawf cocks were as good as Leghorn cocks in natrual mating under floor-rearing system while drawf hens were better than Leghorn hens. Observation results showed no difference on reaction to intermixed hens between drawf layers and Leghorn layers. The best proportion of male to female for drawf layers was 1：8 in this experiment and dwarf cocks tended to mate dwarf hens rather than Leghorn hens.展开更多
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund[CX(12)1001-05]~~
文摘In order to explore a breeding method for mycoplasmal pneumonia-negative swine population, the purifying effects of combination therapy, SEW and three-point breeding and management system on Mycoplasma hyopneumoniae were investigated in this study. After the processes of screening pregnant sows, programmed therapy for sows, SEW, three-point breeding and management in the barrier isolation system and programmed therapy for piglets, the newly-bred piglets were monitored as long as four months by serum antibody detection of Mycoplasma hyopneumoniae and fluorescent quantitative PCR of nose swabs. The results showed that when the newlybred five batches of piglets were older than 35 d, they were all negative by serum antibody detection and nose swab antigen detection. Therefore, the combination therapy, SEW and three-point production and management system can effectively purify Mycoplasma hyopneumoniae, providing theoretical basis and clinical experience for the control and purification of domestic mycoplasmal pneumonia.
基金Project (No. 30300249) supported by the Natural Science Foundationof Guangdong Province, China
文摘Objective:To evaluate the effect and profitability of using the quantitative trait loci (QTL)-linked direct marker (DR marker) in gene-assisted selection (GAS). Methods: Three populations (100, 200, or 300 sows plus 10 boars within each group) with segregating QTL were simulated stochastically. Five economic traits were investigated, including number of born alive (NBA), average daily gain to 100 kg body weight (ADG), feed conversion ratio (FCR), back fat at 100 kg body weight (BF) and intramuscular fat (IMF). Selection was based on the estimated breeding value (EBV) of each trait. The starting frequencies of the QTL's favorable allele were 0.1, 0.3 and 0.5, respectively. The economic return was calculated by gene flow method. Results: The selection efficiency was higher than 100% when DR markers were used in GAS for 5 traits. The selection efficiency for NBA was the highest, and the lowest was for ADG whose QTL had the lowest variance. The mixed model applied DR markers and obtained higher extra genetic gain and extra economic returns. We also found that the lower the frequency of the favorable allele of the QTL, the higher the extra return obtained. Conclusion: GAS is an effective selection scheme to increase the genetic gain and the eco- nomic returns in pig breeding.
基金Supported by the Science and Technology Plan of Qingdao(No.103-4-5-6-jch)the National Natural Science Foundation of China(No.31101916)+1 种基金the Natural Science Foundation of Shandong Province,China(No.ZR2010CM060)the University Student Innovation Plan of Qingdao Agricultural University(No.201030)
文摘Marsupenaeusjaponicus Bate is one of the most valuable cultured shrimp species in China and outdoor earthen pond farming is the most common method of culturing this organism. The need to increase soil usage efficiency in aquaculture has been recognized and a great deal of research effort has been directed toward development of super-intensive farming systems. However, current research and development in this field is largely devoted to Litopenaeus vannamei Boone, while to M.japonicus Bate it has been neglected. In this study, a layered fanning system was designed and a 66-day study was conducted in M.japonicus Bate culture. The system comprised bracket and sand layers that divided a shrimp tank filled to a depth of 1.2 m into four water layers. Conventional tank culture （unlayered） was used as a control. The results show that survival rate, feed conversion efficiency and production of M.japonicus Bate in the layered farming system were 68%, 18%, and 0.59 kg/m^2, respectively, all of which are significantly higher than in the unlayered fanning system （P〈0.01）. These findings confirmed the possibility of using a layered system to culture M. japonicus Bate.
文摘Influenced by animal welfare requirements and expensive labor cost, producers tend to feed layers in non-cage system such as floor rearing. With the development of commercial lines of dwarf layers, the property of dwarf layers＇ natural mating is drawing more and more attention. In this study, the authors hybridized Nongda Ill dwarf layers with White Leghorn chickens in floor-rearing system to research whether dwarf layers had defect in natural mating. Two steps of experiments were performed. The authors detected the fertilization rate of collected eggs, observed chickens＇ behavior in step I when a hen from other group was suddenly put into and recorded copulation number of every group in step II from 7：30 to 19：30. The results indicated that drawf cocks were as good as Leghorn cocks in natrual mating under floor-rearing system while drawf hens were better than Leghorn hens. Observation results showed no difference on reaction to intermixed hens between drawf layers and Leghorn layers. The best proportion of male to female for drawf layers was 1：8 in this experiment and dwarf cocks tended to mate dwarf hens rather than Leghorn hens.
