Aim:The fast and nondestructive identification of Cortex Periplocae and Cortex Acanthopanacis by Fourier transform near infrared spectroscopy and generalized two-dimensional（2D） correlation spectroscopy were perform...Aim:The fast and nondestructive identification of Cortex Periplocae and Cortex Acanthopanacis by Fourier transform near infrared spectroscopy and generalized two-dimensional（2D） correlation spectroscopy were performed.Methods:Near infrared spectra of Cortex Periplocae and Cortex Acanthopanacis was used for computation of the 2D correlation spectra and comparison of the synchronous and asynchronous 2D correlation spectra.Results:Two of the analytes had similar spectral characteristics in one-dimensional near infrared while quite different in 2D correlation spectra.Cortex Periplocae and Cortex Acanthopanacis were identified visually using the 2D correlation spectra in region 5 600～4 700 cm-1and 7 200～6 600 cm-1.Conclusion:The results prove that 2D correlation spectra could enhance the resolution of near infrared spectra and increase the capacity of identification.The combination of 2D correlation spectroscopy and near infrared spectroscopy allow a new and convenient approach for evaluating the medicinal herbs.展开更多
目的:分析中药香加皮醇提物杠柳苷(periplocin from cortex periplocae,CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞周期阻滞的机制。方法:应用MTT法检测CPP对TE-13细胞的抑制作用;Gimsa染色法分析TE-13细胞的形态学变化;FC...目的:分析中药香加皮醇提物杠柳苷(periplocin from cortex periplocae,CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞周期阻滞的机制。方法:应用MTT法检测CPP对TE-13细胞的抑制作用;Gimsa染色法分析TE-13细胞的形态学变化;FCM法检测细胞周期分布和凋亡率;Western印迹法检测TE-13细胞经药物处理前后细胞周期蛋白依赖性激酶CDK4、CDK2蛋白表达的变化。结果:CPP对TE-13细胞增殖具有明显的抑制作用(P<0.01),并呈时间和浓度依赖性,药物浓度越大,作用时间越长,抑制效应越强,CPP作用48h时,对TE-13细胞的半数抑制浓度(IC50)为0.61μg/mL。经2μg/mL的CPP作用48h后,TE-13细胞发生明显的凋亡形态学变化;G0/G1期细胞明显增多(P<0.01),S期细胞明显减少(P<0.01),G2/M期细胞没有明显变化(P>0.05)。不同浓度CPP作用48h后能降低TE-13细胞中CDK4蛋白的表达(P<0.01),对CDK2蛋白的表达则没有明显影响(P>0.05)。结论:CPP能显著抑制TE-13细胞的增殖,其作用机制可能与CPP诱导细胞周期阻滞和细胞凋亡有关。展开更多
目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡...目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡的DNA水平变化;RT-PCR方法检测细胞凋亡相关基因bcl-2、bax和surv iv in mRNA表达水平变化;免疫细胞化学方法检测bcl-2、bax和surv iv in蛋白表达的变化。结果经CPE作用后,人胃癌细胞BGC-823出现明显的细胞凋亡形态学变化及超微结构改变,细胞DNA琼脂糖凝胶电泳呈现梯形图。经250μg/mL CPE处理48 h后,多数BGC-823细胞被阻滞在G2/M期,而且细胞发生明显的凋亡变化,BGC-823细胞凋亡率可达18.9%。CPE可抑制BGC-823细胞bcl和surv iv in mRNA及蛋白的表达,促进baxmRNA及蛋白的表达。CPE可明显延长S180荷瘤小鼠生存期,且具有剂量依赖性。结论CPE通过阻滞BGC-823细胞于G2/M期及诱导BGC-823细胞凋亡发挥抗肿瘤作用,其作用机制与抑制细胞的bcl-2和surv iv in基因mRNA及蛋白表达、促进bax基因和蛋白的表达有关。展开更多
文摘Aim:The fast and nondestructive identification of Cortex Periplocae and Cortex Acanthopanacis by Fourier transform near infrared spectroscopy and generalized two-dimensional（2D） correlation spectroscopy were performed.Methods:Near infrared spectra of Cortex Periplocae and Cortex Acanthopanacis was used for computation of the 2D correlation spectra and comparison of the synchronous and asynchronous 2D correlation spectra.Results:Two of the analytes had similar spectral characteristics in one-dimensional near infrared while quite different in 2D correlation spectra.Cortex Periplocae and Cortex Acanthopanacis were identified visually using the 2D correlation spectra in region 5 600～4 700 cm-1and 7 200～6 600 cm-1.Conclusion:The results prove that 2D correlation spectra could enhance the resolution of near infrared spectra and increase the capacity of identification.The combination of 2D correlation spectroscopy and near infrared spectroscopy allow a new and convenient approach for evaluating the medicinal herbs.
文摘目的:分析中药香加皮醇提物杠柳苷(periplocin from cortex periplocae,CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞周期阻滞的机制。方法:应用MTT法检测CPP对TE-13细胞的抑制作用;Gimsa染色法分析TE-13细胞的形态学变化;FCM法检测细胞周期分布和凋亡率;Western印迹法检测TE-13细胞经药物处理前后细胞周期蛋白依赖性激酶CDK4、CDK2蛋白表达的变化。结果:CPP对TE-13细胞增殖具有明显的抑制作用(P<0.01),并呈时间和浓度依赖性,药物浓度越大,作用时间越长,抑制效应越强,CPP作用48h时,对TE-13细胞的半数抑制浓度(IC50)为0.61μg/mL。经2μg/mL的CPP作用48h后,TE-13细胞发生明显的凋亡形态学变化;G0/G1期细胞明显增多(P<0.01),S期细胞明显减少(P<0.01),G2/M期细胞没有明显变化(P>0.05)。不同浓度CPP作用48h后能降低TE-13细胞中CDK4蛋白的表达(P<0.01),对CDK2蛋白的表达则没有明显影响(P>0.05)。结论:CPP能显著抑制TE-13细胞的增殖,其作用机制可能与CPP诱导细胞周期阻滞和细胞凋亡有关。
文摘目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡的DNA水平变化;RT-PCR方法检测细胞凋亡相关基因bcl-2、bax和surv iv in mRNA表达水平变化;免疫细胞化学方法检测bcl-2、bax和surv iv in蛋白表达的变化。结果经CPE作用后,人胃癌细胞BGC-823出现明显的细胞凋亡形态学变化及超微结构改变,细胞DNA琼脂糖凝胶电泳呈现梯形图。经250μg/mL CPE处理48 h后,多数BGC-823细胞被阻滞在G2/M期,而且细胞发生明显的凋亡变化,BGC-823细胞凋亡率可达18.9%。CPE可抑制BGC-823细胞bcl和surv iv in mRNA及蛋白的表达,促进baxmRNA及蛋白的表达。CPE可明显延长S180荷瘤小鼠生存期,且具有剂量依赖性。结论CPE通过阻滞BGC-823细胞于G2/M期及诱导BGC-823细胞凋亡发挥抗肿瘤作用,其作用机制与抑制细胞的bcl-2和surv iv in基因mRNA及蛋白表达、促进bax基因和蛋白的表达有关。
