Objective The literature has shown that cognitive and emotional changes may occur after chronic treatment with glucocorticoids. This might be caused by the suppressive effect of glucocorticoids on hippocampal neurogen...Objective The literature has shown that cognitive and emotional changes may occur after chronic treatment with glucocorticoids. This might be caused by the suppressive effect of glucocorticoids on hippocampal neurogenesis and cell proliferation. Paroxetine, a selective serotonin reuptake transporter, is a commonly used antidepressant for alleviation of signs and symptoms of clinical depression. It was discovered to promote hippocampal neurogenesis in the past few years and we wanted to investigate its interaction with glucocorticoid in this study. Methods Adult rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine for 14 d. Cell proliferation in the dentate gyrus was quantified using 5-bromo-2-deoxyuridine （BrdU） immunohistochemistry. Results The corticosterone treatment suppressed while paroxetine treatment increased hippocampal cell proliferation. More importantly, paroxetine treatment could reverse the suppressive effect of corticosterone on hippocampal cell proliferation. Conclusion This may have clinic application in preventing hippocampal damage after glucocorticoid treatment.展开更多
Floral organogenesis and development of Przewalskia tangutica Maxim. endemic to China and Hyoscyamus niger L., which belong to the tribe Hyoscyameae (Solanaceae), were studied using scanning electron microscope. They ...Floral organogenesis and development of Przewalskia tangutica Maxim. endemic to China and Hyoscyamus niger L., which belong to the tribe Hyoscyameae (Solanaceae), were studied using scanning electron microscope. They have three common characters of floral organ initiation and development: 1) initiation of the floral organs in the two species follows Hofmeister's rule; 2) the mode of corolla tube development belongs to the 'late sympetaly' type; 3) primordia of the floral appendages initiated in a pentamerous pattern and acropetal order. But initiation of the calyx-lobe primordia showed different modes in these two species. The calyx-lobe primordia of H. niger have simultaneously whorled initiation, while those of P. tangutica have helical initiation, but the five calyx-lobe primordia form a ring after all five calyx-lobe primordia occur. The systematic significance of the present results in the genera Hyoscyamus and Przewalskia is discussed in this paper.展开更多
To address the role of the HCP martensite in CoAl and CoNi shape memory alloys, the relationship between the shape memory effect （SME） and the content of the thermal and stress-induced HCP martensite was invest...To address the role of the HCP martensite in CoAl and CoNi shape memory alloys, the relationship between the shape memory effect （SME） and the content of the thermal and stress-induced HCP martensite was investigated in the solution-treated CoAl and CoNi alloys. In-situ optical observations were employed to investigate the contents of thermal HCP martensite before and after deep cooling and its influence on the stress-induced HCP martensite transformation and SME. The results show that the SME in both the CoAl and the CoNi alloys results from the stress-induced HCP martensite. The role of the thermal HCP martensite in both of them is the strengthening of the matrix. The much higher yield strength in the solution-treated CoAl alloy due to solution strengthening of Al is responsible for its better SME compared with the CoNi alloy.展开更多
In anticipation of the massive burden of neurodegenerative disease within super-aged societies, great efforts have been made to utilize neural stem and progenitor cells for regenerative medicine. The capacity of intri...In anticipation of the massive burden of neurodegenerative disease within super-aged societies, great efforts have been made to utilize neural stem and progenitor cells for regenerative medicine. The capacity of intrinsic neural stem and progenitor cells to regenerate damaged brain tissue remains unclear, due in part to the lack of knowledge about how these newly born neurons integrate into functional circuitry. As sizable integration of adult-born neurons naturally occurs in the dentate gyrus region of the hippocampus, clarifying the mechanisms of this process could provide insights for applying neural stem and progenitor cells in clinical settings. There is convincing evidence of functional correlations between adult-born neurons and memory consolidation and sleep; therefore, we describe some new advances that were left untouched in our recent review.展开更多
The Small ubiquitin-related modifier （SUMO） conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. To investiga...The Small ubiquitin-related modifier （SUMO） conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. To investigate in vivo biological function of SUMO paralogs, we inactivated them in the early development of zebrafish. While zebrafish embryos deficient for all three SUMO paralogs, as Ubc9-deficient ones, displayed severe defects, loss of individual SUMO paralog was compatible with a normal development. SUMO-deficient embryos can be rescued by a single human or zebrafish SUMO. While key structural basic lysine residues and N-terminal unstructured stretch of SUMO are critical for in vivo rescue, the consensus Kll sumoylation site of SUMO2 is dispensable, implying that chain formation on this potential site is unessential for normal development. Inactivation of all three SUMOs triggered p53- dependent apoptosis and further inactivation of p53 restored normal zebrafish development. Interestingly, we also demonstrate that the dominant negative truncated form of p53, Δ113p53, significantly blunts SUMO depletion-induced p53 activity in vivo. Taken together, our results suggest that SUMO paralogs are indispensable, but redundant, in the early development of zebrafish.展开更多
This study investigated the embryonic development of Maldives clownfish Amphiprion nigripes under natural conditions (28 - 30℃) at a lagoon of Agatti Island, Lakshadweep, India. The newly deposited fish egg was capsu...This study investigated the embryonic development of Maldives clownfish Amphiprion nigripes under natural conditions (28 - 30℃) at a lagoon of Agatti Island, Lakshadweep, India. The newly deposited fish egg was capsule-shaped and orange-red, with a (0.73 ± 0.04) mm3 yolk containing 5 - 10 fat globules. The embryonic development of fertilized eggs was divided into 26 stages and the time elapsing for each stage was recorded. Results showed that the cleavage was rapid, with the first division observed 1 h 20 min after fertilization. Blastulation occurred 4 h later, followed by gastrulation 12 h after fertilization, with a yolk volume of (0.61 ± 0.06) mm3. The organogenesis process started 22 h after fertilization when the blastopores closed and notochord formation began. The embryonic stage was recorded 24 h later, with the appearance of forebrain, midbrain, hindbrain, melanophores on yolk-sac and 22 somites, and a decreased yolk volume of (0.54 ± 0.08) mm3. Other organs developed well 31 h after fertilization, whereas the heart started beating and blood circulation began 78 h later. Red pigmentation (erytrophores) appeared 96 h after fertilization, with a small yolk volume of (0.22 ± 0.02) mm3. Mouth developed well and eyes were noticeable 120 h later, with head, pectoral fin and tail frequently moving 144 h after fertilization. The embryo reached the pre-hatching stage 168 h later and started to hatch after 170 - 180 h incubation. This study first detailed the embryonic development and yolk absorption of A. nigripes under natural conditions.展开更多
Mongolian gerbils were used as delayed neuronal damage (DNDi animal models. At the end of 15Abstract:Mongolian gerbils were used as delayed neuronal damage (DND)animal models. At the end of 15 minute cerebral ischemi...Mongolian gerbils were used as delayed neuronal damage (DNDi animal models. At the end of 15Abstract:Mongolian gerbils were used as delayed neuronal damage (DND)animal models. At the end of 15 minute cerebral ischemia and at various reperfusion time ranging from 1 to 96 hours, the content of water and arginine vasopressin (AVP) in the CA1 sector of hippocampus were measured by the specific gravity method and radioimmunoassay. Furthermore, we also examined the effect of intracerebroventricular (ICV) injection of AVP, AVP antiserum on calcium, Na+, K+-ATPase activrty in the CA1 sector after ischemia and 96 hour reperfusion. The results showed that AVP contents of CA1 sector of hippocampus during 6 to 96 hour recirculation, and the water content of CA1 sector during 24 to 96 hour were significantly and continuously increased. After ICV inJection of AVP, the water content and calcium in CA1 sector of hippocampus at cerebral ischemia and 96 hour recirculation further increased, and the Na+, K+- ATPase activity in CA1 sector was remarkably decreased as compared with that of control. While ICV injection of AVP antiserum, the water content and calcium in CA1 sector were significantly decreased as com pared with that of control. These suggested that AVP was involved in the pathophysiologic process of DND in hippocampus following cerebral ischemia and reperfusion. Its mechanism might be through the change of intracellular action mediated by specific AVP receptor to lead to Ca ions over-load of neuron and inhibit the Na+, K+- ATPase activity , thereby to exacerbate the DND in hippocampus.展开更多
Information regarding immunocompetence of the adaptive immune system （AIS） in zebrafish Danio rerio remains limited. Here, we stimulated an immune response in fish embryos, larvae and adults using lipopolysaccharide...Information regarding immunocompetence of the adaptive immune system （AIS） in zebrafish Danio rerio remains limited. Here, we stimulated an immune response in fish embryos, larvae and adults using lipopolysaccharide （LPS） and measured the upregulation of a number of AIS-related genes （Rag2, AID, TCRAC, IgLC-1, mIg, slg, IgZ and DAB） 3 and 18 h later. We found that all of the genes evaluated were strongly induced following LPS stimulation, with most of them responding at 8 d post fertilization. This confirms that a functional adaptive immune response is present in D. rerio larvae, and provides a window for further functional analyses.展开更多
Gastrointestinal stromal tumors (GISTs) are rare tumors, which represent approximately 1% of the neoplasms of the gastrointestinal tract. These tumors rarely give extra-abdominal metastases. However, their clinical ou...Gastrointestinal stromal tumors (GISTs) are rare tumors, which represent approximately 1% of the neoplasms of the gastrointestinal tract. These tumors rarely give extra-abdominal metastases. However, their clinical outcome is potentially adverse. In some rare cases, co- existance of GISTs with other malignancies has been reported. Here we present a case of a 74-year old male with GIST, which was managed by surgical resection. Fourteen months later, the patient presented with liver metastases and imatinib mesylated was administered. During treatment, the patient reported skeletal pain and plane X-rays revealed osteolytic bone lesions. Further investigation revealed the presence of multiple myeloma. To the best of our knowledge, this is the first report of the co-existence of multiple myeloma (MM) with GIST.展开更多
The globe has been confronting a new set of challenges. Such challenges are unique to each country context. The overarching challenge, all above other challenges is, to seek appropriate knowledge as a tool and mean to...The globe has been confronting a new set of challenges. Such challenges are unique to each country context. The overarching challenge, all above other challenges is, to seek appropriate knowledge as a tool and mean to resolve such challenges. A critical such knowledge to be accumulated in all days is on how to develop people or human resources. However, with the existing knowledge claims in this regard, countries have been unable to resolve such challenges. Human Development, one such knowledge claim, is too broader, and therefore, no guided protocol is suggested in finding solutions. Human Resource Development, as another such knowledge claim is too limited in scope to address broader level challenges. National Human Resource Development （NHRD） has emerged to bridge this gap, emphasising people oriented solutions to resolve country level challenges. The NHRD literature encourages NHRD country case studies, and that, justifications for starting NHRD country case studies is needed. This study justifies such a research on NHRD practices in Malaysia＇s country context, using content analysis. As a result, this study has shown higher level country challenges, derived a need of people based approach in resolving such challenges, highlighted NHRD as capable as possible in guiding to establish people development knowledge claim, and finally justified an NHRD research to be done within Malaysia＇s context to see how Malaysia practices NHRD.展开更多
The present paper is the first study conducted in Romania on the inventory of wetlands of a large surface of the country. The focus of this study is the Moldavian Plateau, located in the east of Romania. It is delimit...The present paper is the first study conducted in Romania on the inventory of wetlands of a large surface of the country. The focus of this study is the Moldavian Plateau, located in the east of Romania. It is delimitated by the Eastern Carpathians on the west, the Romanian Plain on the south, the Ukrainian border on the north and the Prut Valley （border with the Republic of Moldavia） on the east. Although the Moldavian Plateau is situated in the driest region of Romania, the majority of the wetlands and of the low discharge rivers is found in this region. The existence of numerous wetlands, respectively small ponds, is influenced by the human activities and the scarcity of water resources. The impermeable clayey substratum favored the occurrence and survival of a large number of wetlands. Most of them are found in the northern Moldavian Plain, and the fewest to the south, in the Covurlui Plateau. The most important wetlands are those developed along the two main rivers draining this territory： Prut and Siret. Unfortunately, Siret River floodplain has been in most of its part protected by means of flood prevention dikes. Consequently, many of the wetlands entered agricultural use.展开更多
Cyclin-dependent kinase 7 （Cdk7） is the catalytic subunit of the metazoan Cdk-activating kinase （CAK）. Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H co...Cyclin-dependent kinase 7 （Cdk7） is the catalytic subunit of the metazoan Cdk-activating kinase （CAK）. Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H complex has been implicated in the regulation ofRNA polymerase in several species, the precise function of their orthologs in zebrafish has not been fully elucidated. In this study, we isolated from zebrafish blastula embryos two cDNAs encoding the orthologs of human Cyclin H and Cdk7, and examined the role of Cdk7/Cyclin H in zebrafish embryogenesis. Sequence analysis showed that the zebrafish Cyclin H and Cdk7 cDNAs encode proteins with 65% and 86% identity to the respective hu- man orthologs. RT-PCR and whole-mount in situ hybridization analyses of their expression in unfertilized eggs, embryos and organs of adult fish suggested that Cyclin H and Cdk7 messages are maternally loaded. Our data also showed that their transcripts were detected throughout development. Distribution of Cyclin H transcripts was found to be ubiquitous during early stages of development and become restricted to the anterior neural tube, brain, eyes, procreate tissues, liver and heart by 5 days post-fertilization. Expression of a dominant-negative form of Cyclin H delayed the onset of zygotic transcription in the early embryo, resulting in apoptosis at 5 hours post-fertilization and leading to sever defects in tis- sues normally exhibiting high levels of Cyclin H expression. These results implicate Cyclin H in the regulation of the transcriptional machinery during midblastula transition and suggest that it is an essential gene in early zebrafish larval development.展开更多
Previous studies have shown that reanalysis products contain large uncertainties in the Tibetan Plateau(TP),and the biases are location dependent.Therefore,these products must be evaluated with in situ observations pr...Previous studies have shown that reanalysis products contain large uncertainties in the Tibetan Plateau(TP),and the biases are location dependent.Therefore,these products must be evaluated with in situ observations prior to their applications.In this study,the authors compare the results of several reanalysis projects with independent sounding observations recorded in the Yarlung Tsangpo River valley in the Eastern Himalayas in June 2010.These reanalysis projects include Climate Forecast System Reanalysis(CFSR),Interim European Centre for Medium Range Weather Forecasts Reanalysis(ERA- Interim),Japanese 25-year Reanalysis(JRA-25),Modern Era Retrospective Analysis for Research and Applications(MERRA),National Center for Environmental Prediction and the Department of Energy Reanalysis 2(NCEP-R2),and NCEP Final Analysis(FNL).Statistical quantities such as average,mean bias(MB),root-mean-square difference(RMSD),and correlation coefficient(R) of temperature,specific humidity,u-wind,and v-wind between 100 hPa and 650 hPa were calculated.The authors determined that the performance of each product differed with variables at different levels.The average profiles of the variables were captured by the reanalysis products,with large biases appearing at lower levels.ERA-Interim and NCEP-R2 showed the best and worst performances,respectively,for all variables.This study suggests that users should select appropriate reanalysis products according to their specific purposes for TP research.展开更多
The dynamics of regional convergence include spatial and temporal dimensions. Spatial Markov chain can be used to explore how regions evolve by considering both individual regions and their geographic neighbors. Based...The dynamics of regional convergence include spatial and temporal dimensions. Spatial Markov chain can be used to explore how regions evolve by considering both individual regions and their geographic neighbors. Based on per capita GDP data set of 77 counties from 1978 to 2000, this paper attempts to investigate the spatial-temporal dynamics of regional convergence in Jiangsu. First, traditional Markov matrix for five per capita GDP classes is constructed for later comparison. Moreover, each region’s spatial lag is derived by averaging all its neighbors’ per capita GDP data. Conditioning on per capita GDP class of its spatial lag at the beginning of each year, spatial Markov transition probabilities of each region are calculated accordingly. Quantitatively, for a poor region, the probability of moving upward is 3.3% if it is surrounded by its poor neighbors, and even increases to 18.4% if it is surrounded by its rich neighbors, but it goes down to 6.2% on average if ignoring regional context. For a rich region, the probability of moving down ward is 1.2% if it is surrounded by its rich neighbors, but increases to 3.0% if it is surrounded by its poor neighbors, and averages 1.5% irrespective of regional context. Spatial analysis of regional GDP class transitions indicates those 10 upward moves of both regions and their neighbors are unexceptionally located in the southern Jiangsu, while downward moves of regions or their neighbors are almost in the northern Jiangsu. These empirical results provide a spatial explanation to the "convergence clubs" detected by traditional Markov chain.展开更多
An experiment was conducted to understand the growth inhibitory effects of aqueous extracts derived from Lantana camara L. （a globally recognized invasive alien weed） on six popular agricultural crops of Bangladesh....An experiment was conducted to understand the growth inhibitory effects of aqueous extracts derived from Lantana camara L. （a globally recognized invasive alien weed） on six popular agricultural crops of Bangladesh. The test was conducted in sterilized petridishes with a photoperiod of 24 hours and an average temperature of 29℃. The effect of different concentrations ofL. camara leaf extracts were recorded and compared with control （i.e., distil water）. Result showed different concentrations of aqueous leaf extracts caused significant inhibitory effect on germination, root and shoot elongation and development of lateral roots of receptor crops. Bioassays also indicated that the inhibitory effect was proportional to the concentrations of the extracts and higher concentration had the stronger inhibitory effect whereas the lower concentration showed stimulatory effect in some cases. The inhibitory effect was much pronounced in root and lateral root development rather than shoot and germination.展开更多
Ecological Marxism Economics is a new economics formation of Marxism. The article, for the first time, putsforward the scientific proposition of establishing Ecological Marxism Economics with distinct Chinese characte...Ecological Marxism Economics is a new economics formation of Marxism. The article, for the first time, putsforward the scientific proposition of establishing Ecological Marxism Economics with distinct Chinese characteristics,and has primarily discussed its theoretic frame and basic contents. Realizing the historic task is the historic mission ofcontemporary Chinese Marxism economists.展开更多
It is impossible to realize the modernization of China without cultural construction. According to the fundamental principles of historic materialism, this paper discusses the relationships between culture and economy...It is impossible to realize the modernization of China without cultural construction. According to the fundamental principles of historic materialism, this paper discusses the relationships between culture and economy in the Chinese socialist construction.展开更多
To compare the effects of CO2-induced seawater acidification and HCI-induced seawater acidification on benthic echinoderms, Hemicentrotus pulcherrimus inhibiting intertidal coastal zone in northern China was utilized ...To compare the effects of CO2-induced seawater acidification and HCI-induced seawater acidification on benthic echinoderms, Hemicentrotus pulcherrimus inhibiting intertidal coastal zone in northern China was utilized in the current study. Under two seawater acidification scenario conditions, embryogenesis and larval spicule development were investigated and compared, respectively. Based on the projection of IPCC, present natural seawater condition （pH=8.06 ± 0.01） and six laboratory-controlled acidified conditions （three CO2-treated groups and three HCI-treated groups, respectively） were set up. Results showed that： （a） early embryonic cleavage tended to be delayed as pH declined in all acidified seawater groups, embryonic cleavage delay of CO2-treated groups tended to be more severe as compared to that of HCI-treated groups; （b） impaired larval symmetry were observed both in CO2-treated groups and HCI-treated groups, there was no significant difference between the two; （c） alteration in skeletal elements was observed in all acidified groups as compared to control, and elongated spicules were observed in CO2-treated groups and shorten spicules were found in HCI-treated groups. Also, when A pH=-0.5, spicule corrosion in CQ-treated group was worse than that in HCI-treated group. All data observed in this study suggest that different seawater acidification scenarios had different effects on sea urchins. Compared to HCI-induced seawater acidification, the effects of CO2-induced seawater acidification on sea urchins were more profound.展开更多
Through studies of the practical reasons for the Charter of Machu Picchu, this paper interprets the core ideas of the Charter, analyzes its historical limitations and proposes several suggestions for current urban and...Through studies of the practical reasons for the Charter of Machu Picchu, this paper interprets the core ideas of the Charter, analyzes its historical limitations and proposes several suggestions for current urban and rural planning.展开更多
文摘Objective The literature has shown that cognitive and emotional changes may occur after chronic treatment with glucocorticoids. This might be caused by the suppressive effect of glucocorticoids on hippocampal neurogenesis and cell proliferation. Paroxetine, a selective serotonin reuptake transporter, is a commonly used antidepressant for alleviation of signs and symptoms of clinical depression. It was discovered to promote hippocampal neurogenesis in the past few years and we wanted to investigate its interaction with glucocorticoid in this study. Methods Adult rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine for 14 d. Cell proliferation in the dentate gyrus was quantified using 5-bromo-2-deoxyuridine （BrdU） immunohistochemistry. Results The corticosterone treatment suppressed while paroxetine treatment increased hippocampal cell proliferation. More importantly, paroxetine treatment could reverse the suppressive effect of corticosterone on hippocampal cell proliferation. Conclusion This may have clinic application in preventing hippocampal damage after glucocorticoid treatment.
文摘Floral organogenesis and development of Przewalskia tangutica Maxim. endemic to China and Hyoscyamus niger L., which belong to the tribe Hyoscyameae (Solanaceae), were studied using scanning electron microscope. They have three common characters of floral organ initiation and development: 1) initiation of the floral organs in the two species follows Hofmeister's rule; 2) the mode of corolla tube development belongs to the 'late sympetaly' type; 3) primordia of the floral appendages initiated in a pentamerous pattern and acropetal order. But initiation of the calyx-lobe primordia showed different modes in these two species. The calyx-lobe primordia of H. niger have simultaneously whorled initiation, while those of P. tangutica have helical initiation, but the five calyx-lobe primordia form a ring after all five calyx-lobe primordia occur. The systematic significance of the present results in the genera Hyoscyamus and Przewalskia is discussed in this paper.
基金Projects(51171123,51271128)supported by the National Natural Science Foundation of China
文摘To address the role of the HCP martensite in CoAl and CoNi shape memory alloys, the relationship between the shape memory effect （SME） and the content of the thermal and stress-induced HCP martensite was investigated in the solution-treated CoAl and CoNi alloys. In-situ optical observations were employed to investigate the contents of thermal HCP martensite before and after deep cooling and its influence on the stress-induced HCP martensite transformation and SME. The results show that the SME in both the CoAl and the CoNi alloys results from the stress-induced HCP martensite. The role of the thermal HCP martensite in both of them is the strengthening of the matrix. The much higher yield strength in the solution-treated CoAl alloy due to solution strengthening of Al is responsible for its better SME compared with the CoNi alloy.
基金partially supported by the MEXT World Premier International Research Center Initiative,CREST JST,MEXT KAKENHI for Scientific Research on Innovative Areas "Microendophenotype"(25116530)and "Memory Dynamism"(26115502)JSPS KAKENHI Grants(16K18359,15F15408)+12 种基金Research Foundation for Opto-Science and TechnologyKato Memorial Bioscience FoundationJapan Foundation for Applied EnzymologyUehara Memorial Foundation2016 Inamori Research Grants ProgramIchiro Kanehara Foundation for the Promotion of Medical Sciences and Medical CareLife Science Foundation of JapanKowa Life Science Foundation Research GrantGSK Japan Research GrantKANAE Foundation for the Promotion of Medical ScienceShimadzu Foundation for the Promotion of Science and TechnologyTakeda Science Foundation to MSThe Tokyo Biochemical Research Foundation to SS
文摘In anticipation of the massive burden of neurodegenerative disease within super-aged societies, great efforts have been made to utilize neural stem and progenitor cells for regenerative medicine. The capacity of intrinsic neural stem and progenitor cells to regenerate damaged brain tissue remains unclear, due in part to the lack of knowledge about how these newly born neurons integrate into functional circuitry. As sizable integration of adult-born neurons naturally occurs in the dentate gyrus region of the hippocampus, clarifying the mechanisms of this process could provide insights for applying neural stem and progenitor cells in clinical settings. There is convincing evidence of functional correlations between adult-born neurons and memory consolidation and sleep; therefore, we describe some new advances that were left untouched in our recent review.
基金Supplementary information is linked to the online version of the paper on the Cell Research website.Acknowledgements We thank Dr Jiang Zhu （Shanghai institute of hematology, Rui Jin hospital） and Dr Nelly Kieffer （CNRS LIA, Rui Jin hospital） for their comments. This work was supported by grants from the National High Tech Program of China （863, 2006AA02Z150）, the National Science Foundation of China （30525006）, the Science and Technology Commission of Shanghai Municipality （07XD14022, 06PJ14068）, ATIP program and BNP PARIBAS.
文摘The Small ubiquitin-related modifier （SUMO） conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. To investigate in vivo biological function of SUMO paralogs, we inactivated them in the early development of zebrafish. While zebrafish embryos deficient for all three SUMO paralogs, as Ubc9-deficient ones, displayed severe defects, loss of individual SUMO paralog was compatible with a normal development. SUMO-deficient embryos can be rescued by a single human or zebrafish SUMO. While key structural basic lysine residues and N-terminal unstructured stretch of SUMO are critical for in vivo rescue, the consensus Kll sumoylation site of SUMO2 is dispensable, implying that chain formation on this potential site is unessential for normal development. Inactivation of all three SUMOs triggered p53- dependent apoptosis and further inactivation of p53 restored normal zebrafish development. Interestingly, we also demonstrate that the dominant negative truncated form of p53, Δ113p53, significantly blunts SUMO depletion-induced p53 activity in vivo. Taken together, our results suggest that SUMO paralogs are indispensable, but redundant, in the early development of zebrafish.
基金the funding from the Ministry of Environment and Forests (India)
文摘This study investigated the embryonic development of Maldives clownfish Amphiprion nigripes under natural conditions (28 - 30℃) at a lagoon of Agatti Island, Lakshadweep, India. The newly deposited fish egg was capsule-shaped and orange-red, with a (0.73 ± 0.04) mm3 yolk containing 5 - 10 fat globules. The embryonic development of fertilized eggs was divided into 26 stages and the time elapsing for each stage was recorded. Results showed that the cleavage was rapid, with the first division observed 1 h 20 min after fertilization. Blastulation occurred 4 h later, followed by gastrulation 12 h after fertilization, with a yolk volume of (0.61 ± 0.06) mm3. The organogenesis process started 22 h after fertilization when the blastopores closed and notochord formation began. The embryonic stage was recorded 24 h later, with the appearance of forebrain, midbrain, hindbrain, melanophores on yolk-sac and 22 somites, and a decreased yolk volume of (0.54 ± 0.08) mm3. Other organs developed well 31 h after fertilization, whereas the heart started beating and blood circulation began 78 h later. Red pigmentation (erytrophores) appeared 96 h after fertilization, with a small yolk volume of (0.22 ± 0.02) mm3. Mouth developed well and eyes were noticeable 120 h later, with head, pectoral fin and tail frequently moving 144 h after fertilization. The embryo reached the pre-hatching stage 168 h later and started to hatch after 170 - 180 h incubation. This study first detailed the embryonic development and yolk absorption of A. nigripes under natural conditions.
文摘Mongolian gerbils were used as delayed neuronal damage (DNDi animal models. At the end of 15Abstract:Mongolian gerbils were used as delayed neuronal damage (DND)animal models. At the end of 15 minute cerebral ischemia and at various reperfusion time ranging from 1 to 96 hours, the content of water and arginine vasopressin (AVP) in the CA1 sector of hippocampus were measured by the specific gravity method and radioimmunoassay. Furthermore, we also examined the effect of intracerebroventricular (ICV) injection of AVP, AVP antiserum on calcium, Na+, K+-ATPase activrty in the CA1 sector after ischemia and 96 hour reperfusion. The results showed that AVP contents of CA1 sector of hippocampus during 6 to 96 hour recirculation, and the water content of CA1 sector during 24 to 96 hour were significantly and continuously increased. After ICV inJection of AVP, the water content and calcium in CA1 sector of hippocampus at cerebral ischemia and 96 hour recirculation further increased, and the Na+, K+- ATPase activity in CA1 sector was remarkably decreased as compared with that of control. While ICV injection of AVP antiserum, the water content and calcium in CA1 sector were significantly decreased as com pared with that of control. These suggested that AVP was involved in the pathophysiologic process of DND in hippocampus following cerebral ischemia and reperfusion. Its mechanism might be through the change of intracellular action mediated by specific AVP receptor to lead to Ca ions over-load of neuron and inhibit the Na+, K+- ATPase activity , thereby to exacerbate the DND in hippocampus.
基金Supported by the Ministry of Science and Technology (MOST) of China (No. 2008AA09Z409)
文摘Information regarding immunocompetence of the adaptive immune system （AIS） in zebrafish Danio rerio remains limited. Here, we stimulated an immune response in fish embryos, larvae and adults using lipopolysaccharide （LPS） and measured the upregulation of a number of AIS-related genes （Rag2, AID, TCRAC, IgLC-1, mIg, slg, IgZ and DAB） 3 and 18 h later. We found that all of the genes evaluated were strongly induced following LPS stimulation, with most of them responding at 8 d post fertilization. This confirms that a functional adaptive immune response is present in D. rerio larvae, and provides a window for further functional analyses.
文摘Gastrointestinal stromal tumors (GISTs) are rare tumors, which represent approximately 1% of the neoplasms of the gastrointestinal tract. These tumors rarely give extra-abdominal metastases. However, their clinical outcome is potentially adverse. In some rare cases, co- existance of GISTs with other malignancies has been reported. Here we present a case of a 74-year old male with GIST, which was managed by surgical resection. Fourteen months later, the patient presented with liver metastases and imatinib mesylated was administered. During treatment, the patient reported skeletal pain and plane X-rays revealed osteolytic bone lesions. Further investigation revealed the presence of multiple myeloma. To the best of our knowledge, this is the first report of the co-existence of multiple myeloma (MM) with GIST.
文摘The globe has been confronting a new set of challenges. Such challenges are unique to each country context. The overarching challenge, all above other challenges is, to seek appropriate knowledge as a tool and mean to resolve such challenges. A critical such knowledge to be accumulated in all days is on how to develop people or human resources. However, with the existing knowledge claims in this regard, countries have been unable to resolve such challenges. Human Development, one such knowledge claim, is too broader, and therefore, no guided protocol is suggested in finding solutions. Human Resource Development, as another such knowledge claim is too limited in scope to address broader level challenges. National Human Resource Development （NHRD） has emerged to bridge this gap, emphasising people oriented solutions to resolve country level challenges. The NHRD literature encourages NHRD country case studies, and that, justifications for starting NHRD country case studies is needed. This study justifies such a research on NHRD practices in Malaysia＇s country context, using content analysis. As a result, this study has shown higher level country challenges, derived a need of people based approach in resolving such challenges, highlighted NHRD as capable as possible in guiding to establish people development knowledge claim, and finally justified an NHRD research to be done within Malaysia＇s context to see how Malaysia practices NHRD.
文摘The present paper is the first study conducted in Romania on the inventory of wetlands of a large surface of the country. The focus of this study is the Moldavian Plateau, located in the east of Romania. It is delimitated by the Eastern Carpathians on the west, the Romanian Plain on the south, the Ukrainian border on the north and the Prut Valley （border with the Republic of Moldavia） on the east. Although the Moldavian Plateau is situated in the driest region of Romania, the majority of the wetlands and of the low discharge rivers is found in this region. The existence of numerous wetlands, respectively small ponds, is influenced by the human activities and the scarcity of water resources. The impermeable clayey substratum favored the occurrence and survival of a large number of wetlands. Most of them are found in the northern Moldavian Plain, and the fewest to the south, in the Covurlui Plateau. The most important wetlands are those developed along the two main rivers draining this territory： Prut and Siret. Unfortunately, Siret River floodplain has been in most of its part protected by means of flood prevention dikes. Consequently, many of the wetlands entered agricultural use.
文摘Cyclin-dependent kinase 7 （Cdk7） is the catalytic subunit of the metazoan Cdk-activating kinase （CAK）. Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H complex has been implicated in the regulation ofRNA polymerase in several species, the precise function of their orthologs in zebrafish has not been fully elucidated. In this study, we isolated from zebrafish blastula embryos two cDNAs encoding the orthologs of human Cyclin H and Cdk7, and examined the role of Cdk7/Cyclin H in zebrafish embryogenesis. Sequence analysis showed that the zebrafish Cyclin H and Cdk7 cDNAs encode proteins with 65% and 86% identity to the respective hu- man orthologs. RT-PCR and whole-mount in situ hybridization analyses of their expression in unfertilized eggs, embryos and organs of adult fish suggested that Cyclin H and Cdk7 messages are maternally loaded. Our data also showed that their transcripts were detected throughout development. Distribution of Cyclin H transcripts was found to be ubiquitous during early stages of development and become restricted to the anterior neural tube, brain, eyes, procreate tissues, liver and heart by 5 days post-fertilization. Expression of a dominant-negative form of Cyclin H delayed the onset of zygotic transcription in the early embryo, resulting in apoptosis at 5 hours post-fertilization and leading to sever defects in tis- sues normally exhibiting high levels of Cyclin H expression. These results implicate Cyclin H in the regulation of the transcriptional machinery during midblastula transition and suggest that it is an essential gene in early zebrafish larval development.
基金supported by the Special Fund for Meteorological Research in the Public Interest (GYHY2012 06041)the National Natural Science Foundation of China (Grant No.40905067)and the Ministry of Science and Technology of the People’s Republic of China (2009CB421403)
文摘Previous studies have shown that reanalysis products contain large uncertainties in the Tibetan Plateau(TP),and the biases are location dependent.Therefore,these products must be evaluated with in situ observations prior to their applications.In this study,the authors compare the results of several reanalysis projects with independent sounding observations recorded in the Yarlung Tsangpo River valley in the Eastern Himalayas in June 2010.These reanalysis projects include Climate Forecast System Reanalysis(CFSR),Interim European Centre for Medium Range Weather Forecasts Reanalysis(ERA- Interim),Japanese 25-year Reanalysis(JRA-25),Modern Era Retrospective Analysis for Research and Applications(MERRA),National Center for Environmental Prediction and the Department of Energy Reanalysis 2(NCEP-R2),and NCEP Final Analysis(FNL).Statistical quantities such as average,mean bias(MB),root-mean-square difference(RMSD),and correlation coefficient(R) of temperature,specific humidity,u-wind,and v-wind between 100 hPa and 650 hPa were calculated.The authors determined that the performance of each product differed with variables at different levels.The average profiles of the variables were captured by the reanalysis products,with large biases appearing at lower levels.ERA-Interim and NCEP-R2 showed the best and worst performances,respectively,for all variables.This study suggests that users should select appropriate reanalysis products according to their specific purposes for TP research.
基金Under the auspices ofthe National Natural Science Foundation of China (No .40301038)
文摘The dynamics of regional convergence include spatial and temporal dimensions. Spatial Markov chain can be used to explore how regions evolve by considering both individual regions and their geographic neighbors. Based on per capita GDP data set of 77 counties from 1978 to 2000, this paper attempts to investigate the spatial-temporal dynamics of regional convergence in Jiangsu. First, traditional Markov matrix for five per capita GDP classes is constructed for later comparison. Moreover, each region’s spatial lag is derived by averaging all its neighbors’ per capita GDP data. Conditioning on per capita GDP class of its spatial lag at the beginning of each year, spatial Markov transition probabilities of each region are calculated accordingly. Quantitatively, for a poor region, the probability of moving upward is 3.3% if it is surrounded by its poor neighbors, and even increases to 18.4% if it is surrounded by its rich neighbors, but it goes down to 6.2% on average if ignoring regional context. For a rich region, the probability of moving down ward is 1.2% if it is surrounded by its rich neighbors, but increases to 3.0% if it is surrounded by its poor neighbors, and averages 1.5% irrespective of regional context. Spatial analysis of regional GDP class transitions indicates those 10 upward moves of both regions and their neighbors are unexceptionally located in the southern Jiangsu, while downward moves of regions or their neighbors are almost in the northern Jiangsu. These empirical results provide a spatial explanation to the "convergence clubs" detected by traditional Markov chain.
文摘An experiment was conducted to understand the growth inhibitory effects of aqueous extracts derived from Lantana camara L. （a globally recognized invasive alien weed） on six popular agricultural crops of Bangladesh. The test was conducted in sterilized petridishes with a photoperiod of 24 hours and an average temperature of 29℃. The effect of different concentrations ofL. camara leaf extracts were recorded and compared with control （i.e., distil water）. Result showed different concentrations of aqueous leaf extracts caused significant inhibitory effect on germination, root and shoot elongation and development of lateral roots of receptor crops. Bioassays also indicated that the inhibitory effect was proportional to the concentrations of the extracts and higher concentration had the stronger inhibitory effect whereas the lower concentration showed stimulatory effect in some cases. The inhibitory effect was much pronounced in root and lateral root development rather than shoot and germination.
文摘Ecological Marxism Economics is a new economics formation of Marxism. The article, for the first time, putsforward the scientific proposition of establishing Ecological Marxism Economics with distinct Chinese characteristics,and has primarily discussed its theoretic frame and basic contents. Realizing the historic task is the historic mission ofcontemporary Chinese Marxism economists.
文摘It is impossible to realize the modernization of China without cultural construction. According to the fundamental principles of historic materialism, this paper discusses the relationships between culture and economy in the Chinese socialist construction.
基金supported by the Liaoning Natural Science Foundation of China(No.20170540104)Open Project of Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea(No.2015-MSENC-KF-01)National Natural Science Foundation of China(NSFC No.41206128)
文摘To compare the effects of CO2-induced seawater acidification and HCI-induced seawater acidification on benthic echinoderms, Hemicentrotus pulcherrimus inhibiting intertidal coastal zone in northern China was utilized in the current study. Under two seawater acidification scenario conditions, embryogenesis and larval spicule development were investigated and compared, respectively. Based on the projection of IPCC, present natural seawater condition （pH=8.06 ± 0.01） and six laboratory-controlled acidified conditions （three CO2-treated groups and three HCI-treated groups, respectively） were set up. Results showed that： （a） early embryonic cleavage tended to be delayed as pH declined in all acidified seawater groups, embryonic cleavage delay of CO2-treated groups tended to be more severe as compared to that of HCI-treated groups; （b） impaired larval symmetry were observed both in CO2-treated groups and HCI-treated groups, there was no significant difference between the two; （c） alteration in skeletal elements was observed in all acidified groups as compared to control, and elongated spicules were observed in CO2-treated groups and shorten spicules were found in HCI-treated groups. Also, when A pH=-0.5, spicule corrosion in CQ-treated group was worse than that in HCI-treated group. All data observed in this study suggest that different seawater acidification scenarios had different effects on sea urchins. Compared to HCI-induced seawater acidification, the effects of CO2-induced seawater acidification on sea urchins were more profound.
文摘Through studies of the practical reasons for the Charter of Machu Picchu, this paper interprets the core ideas of the Charter, analyzes its historical limitations and proposes several suggestions for current urban and rural planning.
