Effects of basic fibroblast growth factor (bFGF) and insulin like growth factor II (IGF II) on cartilage cells from proboscis of skate, Raja porasa Günther, were investigated in this study. The cartilage cells we...Effects of basic fibroblast growth factor (bFGF) and insulin like growth factor II (IGF II) on cartilage cells from proboscis of skate, Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS supplemented MEM medium at 24℃. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.展开更多
文摘Effects of basic fibroblast growth factor (bFGF) and insulin like growth factor II (IGF II) on cartilage cells from proboscis of skate, Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS supplemented MEM medium at 24℃. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.
