目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcr...目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcription 3,p-STAT3)的相关性及与喉癌临床病理因素的关系。方法收集2005年2月～2009年2月在白求恩国际和平医院耳鼻咽喉头颈外科接受手术的LSCC石蜡标本40例,并选取11例正常喉黏膜组织作为对照。采用免疫组化染色法检测SOCS-3、p-STAT3蛋白的表达情况。结果①LSCC组织中SCOS-3蛋白表达明显低于正常喉黏膜组织,两者之间差异具有统计学意义(P=0.000);p-STAT3蛋白在正常喉黏膜组织中的表达小于喉癌组织(P=0.000);②LSCC组织中SOCS-3与p-STAT3蛋白之间存在显著负相关(r=-0.459,P=0.003);③SOCS-3表达与患者年龄(P=0.223)、性别(P=0.849)、肿瘤T分级(P=0.193)无相关性,而与肿瘤病理分期(P=0.0 0 0),淋巴结转移(P=0.0 0 0)有关。结论①在喉正常黏膜组织中SOCS-3均呈阳性表达,而在LSCC组织中部分呈阳性表达;并与p-STAT3蛋白的表达情况呈负相关。提示SOCS-3可能在喉癌生长的过程通过抑制JAK/STAT3信号转导通路的持续活化从而抑制喉癌细胞增长,促进细胞凋亡;②SOCS-3的表达与患者年龄、性别、肿瘤T分级无相关性,而与喉癌病理分期及颈部淋巴结转移有关。展开更多
OBJECTIVE To investigate the prognostic value of measuring Ki67 and VEGF expression in laryngeal squamous cell carcinoma (LSCC). METHODS The expression of Ki67 and VEGF in 32 LSCC tissues was studied by immunohistoche...OBJECTIVE To investigate the prognostic value of measuring Ki67 and VEGF expression in laryngeal squamous cell carcinoma (LSCC). METHODS The expression of Ki67 and VEGF in 32 LSCC tissues was studied by immunohistochemical staining. Of these cases, 5 recurred (recurrent group), 3 cases metastasized (metastatic group), 8 cases died (deceased group) and 24 cased survived (survival group) over a 3 year period of follow-up after their operation. RESULTS The expression of Ki67 and VEGF in the deceased group was higher compared to that in the survival group (P<0.01). Overexpression of Ki67 was found in the recurrent group and in the metastatic group (P< 0.05). VEGF expression was higher in the recurrent group than in the non recurrent group (P<0.05). With Cox-regression of multivariate analysis, Ki67 (RR:3.236; P=0.001), the clinical T stage (RR: 1.382; P=0.029) and metastasis in lymph nodes (RR:0.316; P=0.033) were shown to be independent prognostic factors for survival of LSCC patients. CONCLUSION Ki67 and VEGF expression is related to the prognosis of LSCC. Overexpression of the 2 markers indicated poor prognosis of the disease, a finding which might be helpful for the treatment of laryngocar-cinoma.展开更多
Objective： To study the relationship between Survivin and VEGF proteins in a subgroup of patients with squarnous carcinoma of larynx. Methods： 108 cases of squamous carcinoma of larynx with clinical data were collec...Objective： To study the relationship between Survivin and VEGF proteins in a subgroup of patients with squarnous carcinoma of larynx. Methods： 108 cases of squamous carcinoma of larynx with clinical data were collected and expressions of Survivin and VEGF in peripheral blood were investigated by enzyme-linked immunosorbent assay （ELISA）. Results： Expressions of Survivin and VEGF were significantly associated with T stage, N stage and metastasis of squamous carcinoma of larynx. The patients with Survivin or VEGF over-expressions presented lower survival rate, respectively, as compared to those of low-expression （P 〈 0.05）. The survival rate in squamous carcinoma of larynx patients with Survivin and VEGF dual over-expressions was significantly lower than that of patients with dual low-expression （P 〈 0.05）. Multivariate analysis indicated that both Survivin and VEGF over-expressions in squamous carcinoma of larynx peripheral blood samples were strong independent factors of poor prognosis in squamous carcinoma of larynx patients. Conclusion： Survivin and VEGF over-expressions are independent prognostic factors for the patients with squamous carcinoma of larynx. These results also suggest that peripheral blood Survivin and VEGF expressions are valuable prognostic markers for prognosis prediction in squamous carcinoma of larynx patients.展开更多
文摘目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcription 3,p-STAT3)的相关性及与喉癌临床病理因素的关系。方法收集2005年2月～2009年2月在白求恩国际和平医院耳鼻咽喉头颈外科接受手术的LSCC石蜡标本40例,并选取11例正常喉黏膜组织作为对照。采用免疫组化染色法检测SOCS-3、p-STAT3蛋白的表达情况。结果①LSCC组织中SCOS-3蛋白表达明显低于正常喉黏膜组织,两者之间差异具有统计学意义(P=0.000);p-STAT3蛋白在正常喉黏膜组织中的表达小于喉癌组织(P=0.000);②LSCC组织中SOCS-3与p-STAT3蛋白之间存在显著负相关(r=-0.459,P=0.003);③SOCS-3表达与患者年龄(P=0.223)、性别(P=0.849)、肿瘤T分级(P=0.193)无相关性,而与肿瘤病理分期(P=0.0 0 0),淋巴结转移(P=0.0 0 0)有关。结论①在喉正常黏膜组织中SOCS-3均呈阳性表达,而在LSCC组织中部分呈阳性表达;并与p-STAT3蛋白的表达情况呈负相关。提示SOCS-3可能在喉癌生长的过程通过抑制JAK/STAT3信号转导通路的持续活化从而抑制喉癌细胞增长,促进细胞凋亡;②SOCS-3的表达与患者年龄、性别、肿瘤T分级无相关性,而与喉癌病理分期及颈部淋巴结转移有关。
基金This work was supported by the National Brilliant Youth Science Foundation of China(No. 39925035) Ministry of Education Colleges and Universities Core Teachers Foundation of China (2000), College and University Doctoral Studies Special Purpose Scientific Research Foundation of China(2002).
文摘OBJECTIVE To investigate the prognostic value of measuring Ki67 and VEGF expression in laryngeal squamous cell carcinoma (LSCC). METHODS The expression of Ki67 and VEGF in 32 LSCC tissues was studied by immunohistochemical staining. Of these cases, 5 recurred (recurrent group), 3 cases metastasized (metastatic group), 8 cases died (deceased group) and 24 cased survived (survival group) over a 3 year period of follow-up after their operation. RESULTS The expression of Ki67 and VEGF in the deceased group was higher compared to that in the survival group (P<0.01). Overexpression of Ki67 was found in the recurrent group and in the metastatic group (P< 0.05). VEGF expression was higher in the recurrent group than in the non recurrent group (P<0.05). With Cox-regression of multivariate analysis, Ki67 (RR:3.236; P=0.001), the clinical T stage (RR: 1.382; P=0.029) and metastasis in lymph nodes (RR:0.316; P=0.033) were shown to be independent prognostic factors for survival of LSCC patients. CONCLUSION Ki67 and VEGF expression is related to the prognosis of LSCC. Overexpression of the 2 markers indicated poor prognosis of the disease, a finding which might be helpful for the treatment of laryngocar-cinoma.
基金the grants from the National Outstanding Young Scien- tist Foundation of China (No. 39925035) the National Natural Science Foundations of China (No. 3967078 and No. 30070809)
文摘Objective： To study the relationship between Survivin and VEGF proteins in a subgroup of patients with squarnous carcinoma of larynx. Methods： 108 cases of squamous carcinoma of larynx with clinical data were collected and expressions of Survivin and VEGF in peripheral blood were investigated by enzyme-linked immunosorbent assay （ELISA）. Results： Expressions of Survivin and VEGF were significantly associated with T stage, N stage and metastasis of squamous carcinoma of larynx. The patients with Survivin or VEGF over-expressions presented lower survival rate, respectively, as compared to those of low-expression （P 〈 0.05）. The survival rate in squamous carcinoma of larynx patients with Survivin and VEGF dual over-expressions was significantly lower than that of patients with dual low-expression （P 〈 0.05）. Multivariate analysis indicated that both Survivin and VEGF over-expressions in squamous carcinoma of larynx peripheral blood samples were strong independent factors of poor prognosis in squamous carcinoma of larynx patients. Conclusion： Survivin and VEGF over-expressions are independent prognostic factors for the patients with squamous carcinoma of larynx. These results also suggest that peripheral blood Survivin and VEGF expressions are valuable prognostic markers for prognosis prediction in squamous carcinoma of larynx patients.
