Seven surveys were carried out in April, September, October, December 2006 and March, May, August 2007 in the Yellow Sea, China. Variations in the spatial and temporal distribution of Synechococcus, picoeukaryotes and...Seven surveys were carried out in April, September, October, December 2006 and March, May, August 2007 in the Yellow Sea, China. Variations in the spatial and temporal distribution of Synechococcus, picoeukaryotes and heterotrophic bacteria are quantified using flow cytometry. Synechococcus and heterotrophic bacteria are most abundant from late spring to autumn, while picoeukaryotes concentration is high in spring. Synechococcus and heterotrophic bacteria concentrated high in the northwest part of the Yellow Sea in spring and autumn, while picoeukaryotes distributed evenly over the whole study area except for a small frontal zone in the coastal area on the west （in spring） and central Yellow Sea （in autumn）. Under mixing conditions, the vertical distribution of the three picoplankton groups showed a well-mixed pattern. Upon a well-established stratification, the maximum abundance of picoplankton occurred above the mixed layer depth （-30 m）. Cell sizes of Synechococcus and picoeukaryotes were estimated by converting forward scatter signals （FSC） from cytometry analysis to cell diameter, showing the results of 0.65-0.82 um for Synechococcus and 0.85-1.08 um for picoeukaryotes. The average integrated carbon biomasses ranged 15.26-312.62 mgC/m2 for Synechococcus, 18.54-51.57 mgC/m2 for picoeukaryotes, and 402.63-818.46 mgC/m2 for heterotrophic bacteria. The distribution of Synechococcus and heterotrophic bacteria was temperature dependent, and picoplankton presence was poor in the Yellow Sea Cold Water Mass.展开更多
Tomato yellow leaf curl virus （TYLCV）, belonging to the genus Begomovirus of the family Geminiviridae, is emerging as the most destructive pathogen of tomato plants. Since the first report of TYLCV in Shanghai, Chin...Tomato yellow leaf curl virus （TYLCV）, belonging to the genus Begomovirus of the family Geminiviridae, is emerging as the most destructive pathogen of tomato plants. Since the first report of TYLCV in Shanghai, China in 2006, TYLCV has spread rapidly to 13 provinces or autonomous regions of China. In this study, the molecular varia- bility and evolution of TYLCV were monitored in Shanghai from its first upsurge in 2006 until 2010. Full-length genomic sequences of 26 isolates were obtained by rolling circle amplification. Sequence analysis showed that the intergenic region was the most variable, with a mean mutation rate of 4.81×10-3 nucleotide substitutions per site per year. Ge- netic differentiation was found within isolates obtained from 2006, 2009, and 2010, though a linear increase in genetic diversity over time was not evident. Whilst significant parts of TYLCV genes were under negative selection, the C4 gene embedded entirely within the C1 gene had a tendency to undergo positive selection. Our results indicate that a mechanism of independent evolution of overlapping regions could apply to the natural population of TYLCV in Shanghai, China.展开更多
Soil salinity and hydrologic datasets were assembled to analyze the spatio-temporal variability of salinization in Fengqiu County, Henan Province, China, in the alluvial plain of the lower reaches of the Yellow River....Soil salinity and hydrologic datasets were assembled to analyze the spatio-temporal variability of salinization in Fengqiu County, Henan Province, China, in the alluvial plain of the lower reaches of the Yellow River. The saline soil and groundwater depth data of the county in 1981 were obtained to serve as a historical reference. Electrical conductivity （EC） of 293 surface soil samples taken from 2 kin x 2 km grids in 2007 and 4{） soil profiles acquired in 2（108 was analyzed and used for comparative mapping. Ordinary kriging was applied to predict EC at unobserved locations to derive the horizontal and vertical distribution patterns and variation of soil salinity. Groundwater table data from 22 observation wells in 2008 were collected and used as input for regression kriging to predict the maximum groundwater depth of the county in 2008. Changes in the groundwater level of Fengqiu County in 27 years from 1981 to 2008 was calculated. Two quantitative criteria, the mean error or bias （ME） and the mean squared error （MSE）, were computed to assess the estimation accuracy of the kriging predictions. The results demonstrated that the soil salinity in the upper soil layers decreased dramatically and the taxonomically defined saline soils were present only in a few micro-landscapes after 27 years. Presently, the soils with relatively elevated salt content were mainly distributed in depressions along the Yellow River bed. The reduction in surface soil salinity corresponded to the locations with deepened maximum groundwater depth. It could be concluded that groundwater table recession allowed water to move deeper into the soil profile, transporting salts with it, and thus played an important role in reducing soil salinity in this region. Accumulation of salts in the soil profiles at various depths below the surface indicated that secondary soil salinization would occur when the groundwater was not controlled at a safe depth.展开更多
基金Supported by the National Basic Research Program of China (973 Program) (No.2011CB409804)the National High Technology Research and Development Program of China (863 Program) (No.2007AA09Z434)the Knowledge Innovation Project,CAS (KZCX2-YW-213-3)
文摘Seven surveys were carried out in April, September, October, December 2006 and March, May, August 2007 in the Yellow Sea, China. Variations in the spatial and temporal distribution of Synechococcus, picoeukaryotes and heterotrophic bacteria are quantified using flow cytometry. Synechococcus and heterotrophic bacteria are most abundant from late spring to autumn, while picoeukaryotes concentration is high in spring. Synechococcus and heterotrophic bacteria concentrated high in the northwest part of the Yellow Sea in spring and autumn, while picoeukaryotes distributed evenly over the whole study area except for a small frontal zone in the coastal area on the west （in spring） and central Yellow Sea （in autumn）. Under mixing conditions, the vertical distribution of the three picoplankton groups showed a well-mixed pattern. Upon a well-established stratification, the maximum abundance of picoplankton occurred above the mixed layer depth （-30 m）. Cell sizes of Synechococcus and picoeukaryotes were estimated by converting forward scatter signals （FSC） from cytometry analysis to cell diameter, showing the results of 0.65-0.82 um for Synechococcus and 0.85-1.08 um for picoeukaryotes. The average integrated carbon biomasses ranged 15.26-312.62 mgC/m2 for Synechococcus, 18.54-51.57 mgC/m2 for picoeukaryotes, and 402.63-818.46 mgC/m2 for heterotrophic bacteria. The distribution of Synechococcus and heterotrophic bacteria was temperature dependent, and picoplankton presence was poor in the Yellow Sea Cold Water Mass.
基金supported by the Special Fund for Agro-Scientific Research in the Public Interest from the Ministry of Agriculture,China(No.201003065)the China Postdoctoral Science Foundation(No.2012M510200)the National Natural Science Foundation of China(No.31200117)
文摘Tomato yellow leaf curl virus （TYLCV）, belonging to the genus Begomovirus of the family Geminiviridae, is emerging as the most destructive pathogen of tomato plants. Since the first report of TYLCV in Shanghai, China in 2006, TYLCV has spread rapidly to 13 provinces or autonomous regions of China. In this study, the molecular varia- bility and evolution of TYLCV were monitored in Shanghai from its first upsurge in 2006 until 2010. Full-length genomic sequences of 26 isolates were obtained by rolling circle amplification. Sequence analysis showed that the intergenic region was the most variable, with a mean mutation rate of 4.81×10-3 nucleotide substitutions per site per year. Ge- netic differentiation was found within isolates obtained from 2006, 2009, and 2010, though a linear increase in genetic diversity over time was not evident. Whilst significant parts of TYLCV genes were under negative selection, the C4 gene embedded entirely within the C1 gene had a tendency to undergo positive selection. Our results indicate that a mechanism of independent evolution of overlapping regions could apply to the natural population of TYLCV in Shanghai, China.
基金Supported by the Innovation and Cutting-Edge Project of the Institute of Soil Science, Chinese Academy of Sciences (No. ISSASIP0716)the National Natural Science Foundation of China (No. 40701070)the Knowledge Innovation Project of the Chinese Academy of Sciences (No. KSCX1-YW-09-02)
文摘Soil salinity and hydrologic datasets were assembled to analyze the spatio-temporal variability of salinization in Fengqiu County, Henan Province, China, in the alluvial plain of the lower reaches of the Yellow River. The saline soil and groundwater depth data of the county in 1981 were obtained to serve as a historical reference. Electrical conductivity （EC） of 293 surface soil samples taken from 2 kin x 2 km grids in 2007 and 4{） soil profiles acquired in 2（108 was analyzed and used for comparative mapping. Ordinary kriging was applied to predict EC at unobserved locations to derive the horizontal and vertical distribution patterns and variation of soil salinity. Groundwater table data from 22 observation wells in 2008 were collected and used as input for regression kriging to predict the maximum groundwater depth of the county in 2008. Changes in the groundwater level of Fengqiu County in 27 years from 1981 to 2008 was calculated. Two quantitative criteria, the mean error or bias （ME） and the mean squared error （MSE）, were computed to assess the estimation accuracy of the kriging predictions. The results demonstrated that the soil salinity in the upper soil layers decreased dramatically and the taxonomically defined saline soils were present only in a few micro-landscapes after 27 years. Presently, the soils with relatively elevated salt content were mainly distributed in depressions along the Yellow River bed. The reduction in surface soil salinity corresponded to the locations with deepened maximum groundwater depth. It could be concluded that groundwater table recession allowed water to move deeper into the soil profile, transporting salts with it, and thus played an important role in reducing soil salinity in this region. Accumulation of salts in the soil profiles at various depths below the surface indicated that secondary soil salinization would occur when the groundwater was not controlled at a safe depth.
