黑麂,为我国特有动物,被公认为目前世界上最珍稀的鹿科动物之一。为了更好地保护黑麂这一珍稀的濒危野生动物,基于黑麂线粒体控制区序列对遂昌分布中心的3个黑麂种群的遗传多样性和基因流进行了研究。结果显示,3个种群的36个个体中有13...黑麂,为我国特有动物,被公认为目前世界上最珍稀的鹿科动物之一。为了更好地保护黑麂这一珍稀的濒危野生动物,基于黑麂线粒体控制区序列对遂昌分布中心的3个黑麂种群的遗传多样性和基因流进行了研究。结果显示,3个种群的36个个体中有13个变异位点,占分析序列长度的2.71%,且这13个变异位点皆为碱基置换,并未出现碱基插入或缺失的现象,并由此定义了12个单倍型。遗传多样性检测结果显示遂昌种群具有最高的遗传多样性,应予以优先保护。从Tajima′sD和Fu and Li′sD值的估算结果来看,这3个黑麂种群相对于中性进化的歧异度并没有明显的偏离(P>0.1),没有明显的证据显示这3个黑麂种群间存在很强的平衡选择。3个种群间基因流Nm均大于1,这3个黑麂种群间存在着较为丰富的基因流。3个黑麂种群单倍型间的序列差异为0.009,这表明这3个黑麂种群的单倍型未出现分化。展开更多
We s tudied seasonal habitat use of black muntjacs in the Jiulong Mount ain and Gutian Mountain Nature Reserves in Zhejiang Province, April 2002-Decembe r 2003 based on indirect sign (e.g., tracks, feces). We assumed ...We s tudied seasonal habitat use of black muntjacs in the Jiulong Mount ain and Gutian Mountain Nature Reserves in Zhejiang Province, April 2002-Decembe r 2003 based on indirect sign (e.g., tracks, feces). We assumed that abun dance of sign functioned as a surrogate for intensity of use. During the 20-mon t h sampling period, we quantified 11 ecological factors in 141 sample plots. Black muntjac vegetation use varied significantly with season. Variati on in muntjac use of vegetation types were primarily related to seasonal changes of food resources and concealment conditions. Durin g spring, autumn and winter, muntjacs mostly used middle and lower portions of mountainsides, whereas they used upper portions of mountains during summer. Varying seasonal use of elevation mos t likely accounted for slope effects. We found no evidence of seasonal va riation in muntjac use of slope, aspect, rock exposur e, distance from water or level of human disturbance.展开更多
以流式细胞仪分离小麂(Muntiacus reevesi)Y染色体和黑麂(Muntiacus crinifrons)Y1,Y2,X+4和1号染色体,利用DOP-PCR技术富集了分离的各单条染色体。然后,将小麂的Y染色体的DOP-PCR产物经Cy3标记后直接作为涂染探针, 应用染色体涂染技术...以流式细胞仪分离小麂(Muntiacus reevesi)Y染色体和黑麂(Muntiacus crinifrons)Y1,Y2,X+4和1号染色体,利用DOP-PCR技术富集了分离的各单条染色体。然后,将小麂的Y染色体的DOP-PCR产物经Cy3标记后直接作为涂染探针, 应用染色体涂染技术与雌雄黑麂的核型标本进行杂交,确认了黑麂真正的Y染色体为Y2染色体。再以黑麂的Y1,Y2,X+4和1号染色体的DOP-PCR产物为模板,用人的特异性的 SRY（sex determining region of the Y chromosome ） 基因引物对其进行扩增,结果表明黑麂只有Y2染色体出现了SRY扩增片段。然后扩增产物克隆和测序,比较它与人的同源性,初步把黑麂的Sry基因定位在Y2染色体上。最后提取雄性黑麂的基因组DNA,并用同一对引物对其进行扩增,亦得到Sry基因的片段,对此扩增片段进行克隆,测序,结果表明其与Y2染色体得到的Sry基因片段完全一样，与人SRY基因的同源性均为83%。展开更多
文摘黑麂,为我国特有动物,被公认为目前世界上最珍稀的鹿科动物之一。为了更好地保护黑麂这一珍稀的濒危野生动物,基于黑麂线粒体控制区序列对遂昌分布中心的3个黑麂种群的遗传多样性和基因流进行了研究。结果显示,3个种群的36个个体中有13个变异位点,占分析序列长度的2.71%,且这13个变异位点皆为碱基置换,并未出现碱基插入或缺失的现象,并由此定义了12个单倍型。遗传多样性检测结果显示遂昌种群具有最高的遗传多样性,应予以优先保护。从Tajima′sD和Fu and Li′sD值的估算结果来看,这3个黑麂种群相对于中性进化的歧异度并没有明显的偏离(P>0.1),没有明显的证据显示这3个黑麂种群间存在很强的平衡选择。3个种群间基因流Nm均大于1,这3个黑麂种群间存在着较为丰富的基因流。3个黑麂种群单倍型间的序列差异为0.009,这表明这3个黑麂种群的单倍型未出现分化。
文摘We s tudied seasonal habitat use of black muntjacs in the Jiulong Mount ain and Gutian Mountain Nature Reserves in Zhejiang Province, April 2002-Decembe r 2003 based on indirect sign (e.g., tracks, feces). We assumed that abun dance of sign functioned as a surrogate for intensity of use. During the 20-mon t h sampling period, we quantified 11 ecological factors in 141 sample plots. Black muntjac vegetation use varied significantly with season. Variati on in muntjac use of vegetation types were primarily related to seasonal changes of food resources and concealment conditions. Durin g spring, autumn and winter, muntjacs mostly used middle and lower portions of mountainsides, whereas they used upper portions of mountains during summer. Varying seasonal use of elevation mos t likely accounted for slope effects. We found no evidence of seasonal va riation in muntjac use of slope, aspect, rock exposur e, distance from water or level of human disturbance.
文摘以流式细胞仪分离小麂(Muntiacus reevesi)Y染色体和黑麂(Muntiacus crinifrons)Y1,Y2,X+4和1号染色体,利用DOP-PCR技术富集了分离的各单条染色体。然后,将小麂的Y染色体的DOP-PCR产物经Cy3标记后直接作为涂染探针, 应用染色体涂染技术与雌雄黑麂的核型标本进行杂交,确认了黑麂真正的Y染色体为Y2染色体。再以黑麂的Y1,Y2,X+4和1号染色体的DOP-PCR产物为模板,用人的特异性的 SRY（sex determining region of the Y chromosome ） 基因引物对其进行扩增,结果表明黑麂只有Y2染色体出现了SRY扩增片段。然后扩增产物克隆和测序,比较它与人的同源性,初步把黑麂的Sry基因定位在Y2染色体上。最后提取雄性黑麂的基因组DNA,并用同一对引物对其进行扩增,亦得到Sry基因的片段,对此扩增片段进行克隆,测序,结果表明其与Y2染色体得到的Sry基因片段完全一样，与人SRY基因的同源性均为83%。
