Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flowe...Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour.‘Shixia’,an economically important fruit crop in subtropical regions,we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci(PHAS loci),120 hairpin loci,producing abundant sRNAs,were identified by in-house protocols.Our results suggested that 56 miRNA-target pairs,2221-nt-PHAS loci,and 111 hairpin loci are involved in posttranscriptional gene silencing during longan reproductive development.Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled,including miR482-PHAS and miRN15.miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense,and miRN15 is a novel miRNA likely associated with a hairpin locus(HPL-056)to regulate strigolactone receptor gene DWARF14(D14)and the biogenesis of phasiRNAs from D14.These small RNAs are enriched in flower buds,suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.展开更多
Longan originates from southern China and has high nutritional and health value.Recent phytochemistry and pharmacology studies have shown that polysaccharides are a main bioactive component of longan.Longan polysaccha...Longan originates from southern China and has high nutritional and health value.Recent phytochemistry and pharmacology studies have shown that polysaccharides are a main bioactive component of longan.Longan polysaccharides possess antioxidant,anti-aging,anti-tumor,immunomodulatory,and other bioactivities.Hot-water extraction,ethanol precipitation,and ultrasonic extraction are generally used to extract water-soluble longan polysaccharides.However,the relationship between the structure and bioactivity of longan polysaccharides remains unclear,requiring further investigation.The aim of this review is to evaluate the current literature focusing on the extraction,purification,structural characterization,and biological activity of longan polysaccharides.We believe that this review would provide a useful bibliography for further innovation and a basis for using longan polysaccharides in functional food.展开更多
基金funded by the National Key Research and Developmental Program of China(Grant No.2018YFD1000104)supported by awards from the National Natural Science Foundation of China(Grant Nos.32002009 and 32072547)The Special Support Program of Guangdong Province(Grant No.2019TX05N193).
文摘Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour.‘Shixia’,an economically important fruit crop in subtropical regions,we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci(PHAS loci),120 hairpin loci,producing abundant sRNAs,were identified by in-house protocols.Our results suggested that 56 miRNA-target pairs,2221-nt-PHAS loci,and 111 hairpin loci are involved in posttranscriptional gene silencing during longan reproductive development.Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled,including miR482-PHAS and miRN15.miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense,and miRN15 is a novel miRNA likely associated with a hairpin locus(HPL-056)to regulate strigolactone receptor gene DWARF14(D14)and the biogenesis of phasiRNAs from D14.These small RNAs are enriched in flower buds,suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.
基金the National Natural Science Foundation of China(32201969,82204668)Natural Science Foundation of Henan Province(212300410297)+3 种基金Hebei Natural Science Foundation(H2022423376)Basic Research Plan of Higher Education School Key Scientific Research Project of Henan Province(21A550014)Doctoral Research Foundation of Zhengzhou University of Light Industry(2020BSJJ015)Science and Technology Research Project of Higher Education in Hebei Province(QN2020233).
文摘Longan originates from southern China and has high nutritional and health value.Recent phytochemistry and pharmacology studies have shown that polysaccharides are a main bioactive component of longan.Longan polysaccharides possess antioxidant,anti-aging,anti-tumor,immunomodulatory,and other bioactivities.Hot-water extraction,ethanol precipitation,and ultrasonic extraction are generally used to extract water-soluble longan polysaccharides.However,the relationship between the structure and bioactivity of longan polysaccharides remains unclear,requiring further investigation.The aim of this review is to evaluate the current literature focusing on the extraction,purification,structural characterization,and biological activity of longan polysaccharides.We believe that this review would provide a useful bibliography for further innovation and a basis for using longan polysaccharides in functional food.
