Objective： The aim of the study was to investigate the association of esophageal squamous cell carcinoma （ESCC） genetic susceptibility with the single nucleotide polymorphism （SNP） rs679620 （-Lys45Glu-） in exon...Objective： The aim of the study was to investigate the association of esophageal squamous cell carcinoma （ESCC） genetic susceptibility with the single nucleotide polymorphism （SNP） rs679620 （-Lys45Glu-） in exon 2 of the romp3 gene, and the population in high incidence region of Henan （China） was selected for exploring the mechanism by case-control study, Methods： The romp3 SNP was genotyped by PCR-RFLP analysis in total 605 cases of Henan population, in which there were 227 ESCC cases and 197 controls of An-yang in Henan plus 181 controls of emigrants in Hubei from Xi-chuan of Henan, China. Results： The statistic data showed that GIG and G/A genotype frequencies of SNP rs679620 were significantly different between the controls of emigrants of Xi-chuan in Hubei and controls of An-yang in Henan （P 〈 0.01） also the ESCC cases of An-yang in Henan （P 〈 0.01）, respectively. Conclusion： This study suggests that the SNP rs679620 （-Lys45Glu-） in exon 2 of the mmp3 gene might be associated with ESCC genetic susceptibility.展开更多
Objective To determine HPV infection status and expression of p16, cyclooxygenase-2(COX-2), Cyclin D1 and epidermal growth factor receptor(EGFR) in ESCC patients of Pakistan. Methods 51 ESCC patients treated at Aga Kh...Objective To determine HPV infection status and expression of p16, cyclooxygenase-2(COX-2), Cyclin D1 and epidermal growth factor receptor(EGFR) in ESCC patients of Pakistan. Methods 51 ESCC patients treated at Aga Khan University Hospital(AKUH) were included. HPV infection status was confirmed via PCR while the expression of the four biomarkers was determined using immunohistochemistry. The correlation of all markers with patient clinicopathological features as well as each other was analyzed. Results HPV infection status and p16 overexpression was found to be negative in all the patients while COX-2, Cyclin D1 and EGFR were overexpressed in 56.9%, 62.7% and 49.0% of the patients respectively. COX-2 showed a significant correlation with tumor invasion while EGFR was shown to be significantly correlated with histological grading and COX-2 expression. Conclusion COX-2 and EGFR can be used as prognostic markers. HPV does not seem to be a causative agent for ESCC in Pakistan.展开更多
基金Supported by a grant from the Natural Sciences Foundation of State Ethnic Affairs Commission of China (No. 07ZN09)
文摘Objective： The aim of the study was to investigate the association of esophageal squamous cell carcinoma （ESCC） genetic susceptibility with the single nucleotide polymorphism （SNP） rs679620 （-Lys45Glu-） in exon 2 of the romp3 gene, and the population in high incidence region of Henan （China） was selected for exploring the mechanism by case-control study, Methods： The romp3 SNP was genotyped by PCR-RFLP analysis in total 605 cases of Henan population, in which there were 227 ESCC cases and 197 controls of An-yang in Henan plus 181 controls of emigrants in Hubei from Xi-chuan of Henan, China. Results： The statistic data showed that GIG and G/A genotype frequencies of SNP rs679620 were significantly different between the controls of emigrants of Xi-chuan in Hubei and controls of An-yang in Henan （P 〈 0.01） also the ESCC cases of An-yang in Henan （P 〈 0.01）, respectively. Conclusion： This study suggests that the SNP rs679620 （-Lys45Glu-） in exon 2 of the mmp3 gene might be associated with ESCC genetic susceptibility.
基金supported by Seed Money Grant,Aga Khan University Hospital,awarded to Dr.Syed Muhammad Adnan Ali.Grant ID:80095
文摘Objective To determine HPV infection status and expression of p16, cyclooxygenase-2(COX-2), Cyclin D1 and epidermal growth factor receptor(EGFR) in ESCC patients of Pakistan. Methods 51 ESCC patients treated at Aga Khan University Hospital(AKUH) were included. HPV infection status was confirmed via PCR while the expression of the four biomarkers was determined using immunohistochemistry. The correlation of all markers with patient clinicopathological features as well as each other was analyzed. Results HPV infection status and p16 overexpression was found to be negative in all the patients while COX-2, Cyclin D1 and EGFR were overexpressed in 56.9%, 62.7% and 49.0% of the patients respectively. COX-2 showed a significant correlation with tumor invasion while EGFR was shown to be significantly correlated with histological grading and COX-2 expression. Conclusion COX-2 and EGFR can be used as prognostic markers. HPV does not seem to be a causative agent for ESCC in Pakistan.
