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Development of Ecological Absorbent Core Sanitary Pads in Combination of Kenaf and Chitosan Fibers
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作者 Desh Maruthi Jeyakanthan Jens Schuster Yousuf Pasha Shaik 《Materials Sciences and Applications》 2023年第7期363-381,共19页
The menstrual cycle is always considered as a big nightmare by many women. This research aims to make this process smooth and safe by developing natural sanitary pads which are used to absorb and retain menstrual bloo... The menstrual cycle is always considered as a big nightmare by many women. This research aims to make this process smooth and safe by developing natural sanitary pads which are used to absorb and retain menstrual blood from the body. Some existing sanitary pads contain 90% plastics made of non-woven polypropylene/polyethylene sheets, super absorbent polymers, and polyethylene back sheets that will take up to 600 - 800 years to decompose. So, biodegradable sanitary pads using natural fibers are the best alternative to eliminate the pads which contain non-biodegradable materials. In this research, nonwoven bamboo will be used as the top layer, nonwoven cotton will be used as the second layer, the absorbent core is to be made by the combination of kenaf and chitosan fibers as the third layer, cotton as the fourth layer, and cornstarch-based bioplastic sheets as the bottom layer. These biodegradable natural materials will change the menstrual process into a healthy one as well as create a robust ecological community. 展开更多
关键词 Sanitary Pads BIODEGRADABLE kenaf CHITOSAN BAMBOO Cotton Corn-Based Polylactic Acid
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红麻HcKAN4基因克隆、表达及在类黄酮合成中的功能
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作者 吴法轩 李秦 +6 位作者 杨昕 李新根 徐建堂 陶爱芬 方平平 祁建民 张立武 《作物学报》 CAS CSCD 北大核心 2024年第3期645-655,共11页
MYB类转录因子KAN4(KANADI4)在红麻类黄酮合成和纤维发育中发挥着重要作用。本研究以红麻品种‘福红952’为材料,对HcKAN4基因进行克隆和表达模式分析,探讨TRV-VIGS诱导该基因沉默对类黄酮合成途径中关键酶基因表达量改变的影响。基因... MYB类转录因子KAN4(KANADI4)在红麻类黄酮合成和纤维发育中发挥着重要作用。本研究以红麻品种‘福红952’为材料,对HcKAN4基因进行克隆和表达模式分析,探讨TRV-VIGS诱导该基因沉默对类黄酮合成途径中关键酶基因表达量改变的影响。基因克隆显示,HcKAN4基因开放阅读框(open reading frame,ORF)全长为966 bp,编码322个氨基酸,包含一个MYB保守结构域。进化树分析发现,其与拟南芥和木槿KAN4s的亲缘关系较近。表达分析表明,该基因在红麻不同组织中均有表达,且转录水平随着植物生长而递增。VIGS诱导基因沉默显示,6株HcKAN4的转录水平显著下调,达到基因沉默效果。进一步实时荧光定量PCR检测发现,类黄酮合成相关基因HcCHS、HcF3’5’H、HcANS、HcANR的转录水平显著下调,分别是对照组的0.51、0.14、0.23、0.11倍,表明HcKAN4基因可调控红麻类黄酮生物合成相关基因。这些结果为阐明红麻MYB转录因子调控类黄酮合成提供了依据,同时为改善纤维品质提供了研究思路。 展开更多
关键词 红麻 HcKAN4 类黄酮合成 VIGS
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不同浓度硅提高红麻抗旱机理研究
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作者 王婧 王雨千 刘立军 《中国麻业科学》 2024年第3期162-168,共7页
红麻(Hibiscus cannabinus L.)是我国重要的经济作物,主要分布在安徽、福建、河南等地区,其纤维和茎秆具有丰富的应用价值。随着红麻种植区域向干旱山坡地转移,其抗逆性研究变得尤为关键。研究通过称重法控制水分,模拟干旱环境,选取“红... 红麻(Hibiscus cannabinus L.)是我国重要的经济作物,主要分布在安徽、福建、河南等地区,其纤维和茎秆具有丰富的应用价值。随着红麻种植区域向干旱山坡地转移,其抗逆性研究变得尤为关键。研究通过称重法控制水分,模拟干旱环境,选取“红优2号”作为试验品种,通过盆栽试验进行干旱处理,设置3个硅浓度(0.2、0.5、0.8 g/L,标记为Si0.2、Si0.5、Si0.8),探究硅浓度对不同生长期红麻在干旱胁迫条件下的生理响应和生长状况的影响,以及硅对红麻抗旱性能的潜在调控机制。结果显示,喷施0.5 g/L硅溶液对株高和茎粗的效果最佳,分别较干旱处理增加50.00%和22.76%。综合对叶片结构和生理指标的影响,以0.5 g/L的硅溶液喷施效果最佳。试验证实了硅在提高红麻抗旱性方面的积极作用,为提升红麻在全生育期的抗旱能力提供了实践指导和理论依据。 展开更多
关键词 红麻 干旱胁迫 生长发育
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5种杀菌剂对红麻炭疽病防效及红麻产量的影响
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作者 杨光 何世界 +3 位作者 张丽霞 史鹏飞 刘耀 潘兹亮 《扬州大学学报(农业与生命科学版)》 CAS 北大核心 2024年第4期130-136,共7页
为筛选防治红麻炭疽病的高效杀菌剂,采用生长速率法和凹玻片法室内测定5种杀菌剂对红麻炭疽病菌菌丝生长和孢子萌发的毒力,选择其中3种效果较好的药剂进行田间药效试验。结果表明:咪鲜胺、吡唑醚酯和苯醚甲环唑对红麻炭疽病菌菌丝生长... 为筛选防治红麻炭疽病的高效杀菌剂,采用生长速率法和凹玻片法室内测定5种杀菌剂对红麻炭疽病菌菌丝生长和孢子萌发的毒力,选择其中3种效果较好的药剂进行田间药效试验。结果表明:咪鲜胺、吡唑醚酯和苯醚甲环唑对红麻炭疽病菌菌丝生长和孢子萌发的抑制效果较好,菌丝的EC50分别为0.0880、0.1205和0.5136μg·mL^(-1),孢子萌发的EC50分别为0.1230、0.1658和0.8987μg·mL^(-1)。以25%咪鲜胺乳油900 mL·hm^(-2)剂量(用水量为450 kg·hm^(-2))对红麻叶面进行喷施,对红麻炭疽病田间防效达94.34%,且使红麻单株纤维重较对照显著增加15.92%。这一研究可为红麻炭疽病的防治提供参考。 展开更多
关键词 红麻 炭疽病 杀菌剂 毒力测定 田间防治
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洋麻纤维地聚合物混凝土的抗渗透及抗腐蚀性能研究
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作者 孙本佳 《交通科技》 2024年第4期151-156,共6页
混凝土中掺加纤维能够改善地聚合物混凝土的拉伸性能,添加天然纤维替代合成纤维更符合低碳环保理念,而洋麻纤维便是其中之一。文中以地聚合物混凝土为研究对象,通过开展物理力学实验及吸水性试验,分析洋麻纤维对地聚合物混凝土吸水性、... 混凝土中掺加纤维能够改善地聚合物混凝土的拉伸性能,添加天然纤维替代合成纤维更符合低碳环保理念,而洋麻纤维便是其中之一。文中以地聚合物混凝土为研究对象,通过开展物理力学实验及吸水性试验,分析洋麻纤维对地聚合物混凝土吸水性、孔隙率、抗氯离子渗透,以及抗腐蚀的能力。结果表明,随着洋麻纤维长度和含量不断增加,地聚合物混凝土的吸水率、孔隙度,以及吸附性也随之提高;0.75%,1.0%纤维含量和20,30 mm条件下洋麻纤维地聚合物混凝土,抗水渗透能力较好,比普通地聚合物混凝土氯离子渗透深度减少20%~25%;洋麻纤维地聚合物混凝土在硫酸盐和酸性环境中表现出抗硫酸盐和酸性侵蚀能力,但当将纤维含量为1.5%、长度40 mm时候,抗酸性能力有所降低。 展开更多
关键词 洋麻纤维 地聚合物复合材料 耐久性 耐酸性
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Assessment of Pb uptake,translocation and immobilization in kenaf (Hibiscus cannabinus L.) for phytoremediation of sand tailings 被引量:15
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作者 HO Wai Mun ANG Lai Hoe LEE Don Koo 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2008年第11期1341-1347,共7页
The potential of kenaf (Hibiscus cannabinus L.) for phytoremediation of lead (Pb) on sand tailings was investigated.A pot experiment employing factorial design with two main effects of fertilizer and lead was conducte... The potential of kenaf (Hibiscus cannabinus L.) for phytoremediation of lead (Pb) on sand tailings was investigated.A pot experiment employing factorial design with two main effects of fertilizer and lead was conducted in a nursery using sand tailings from an ex-tin mine as the growing medium.Results showed that Pb was found in the root,stem,and seed capsule of kenaf but not in the leaf.Application of organic fertilizer promoted greater biomass yield as well as higher accumulation capacity of Pb.In Pb-spike... 展开更多
关键词 kenaf lead FERTILIZER bioaccumulation capacity PHYTOREMEDIATION
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Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings 被引量:14
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作者 LI Feng-tao QI Jian-min +4 位作者 ZHANG Gao-yang LIN Li-hui FANG Ping-ping TAO Ai-fen XU Jian-tang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第4期610-620,共11页
The effects of cadmium stress on the growth, antioxidative enzymes and lipid peroxidation in two kenaf plants, Fuhong 991 and ZM412, were analysed under control (0.5-strength Hoagland's nutrient solution) or five l... The effects of cadmium stress on the growth, antioxidative enzymes and lipid peroxidation in two kenaf plants, Fuhong 991 and ZM412, were analysed under control (0.5-strength Hoagland's nutrient solution) or five levels of cadmium stress (0.5- strength Hoagland's nutrient solution containing different concentrations of Cd2+). The leaves and roots of control and cadmium-stressed plants were harvested after 3 wk. At the same Cd concentration, the Cd tolerance index of Fuhong 991 was higher than that of ZM412, indicating that Fuhong 991 may be more tolerant to Cd than ZM412. The superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD) activities fluctuated in the leaves of the Cd-stressed plants compared to the control, whereas the glutathione reductase activity (GR) was much larger than the control for Fuhong 991, ensuring that sufficient quantities of GSH were available to respond to the cadmium stress. In comparison to the control, the dynamic tendency of the SOD, CAT and POD activities in roots of the Cd-stressed plants all increased and then declined, but the POD activity of Fuhong 991 remained nearly unchanged at all of the stress levels. The increase in the enzyme activities demonstrated that Fuhong 991 was more tolerant to cadmium than ZM 412. The lipid peroxidation was enhanced only in the leaves of Cd-stressed ZM 412. These findings indicated that antioxidative activities may play important roles in Cd-stressed Fuhong 991 and ZM 412 and that the leaf and root cell membranes of Fuhong 991 have a greater stability than those of ZM 412. For pollution monitoring purposes, the GR activity in the roots and leaves may serve as a biomarker of Cd for Fuhong 991, whereas lipid peroxidation may serve as biomarker for ZM 412. 展开更多
关键词 kenaf cadmium stress superoxide dismutase (SOD) catalase (CAT) peroxidase (POD) glutathione reductase(GR) glutathione (GSH)
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Optimization of Two-Dimensional Gel Electrophoresis for Kenaf Leaf Proteins 被引量:8
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作者 CHEN Tao QI Jian-min XU Jian-tang CHEN Pin-pin TAO Ai-fen CHEN Fu-cheng CHEN Wei 《Agricultural Sciences in China》 CAS CSCD 2011年第12期1842-1850,共9页
To establish a suitable and effective protocol of protein extraction for two-dimensional gel electrophoresis (2-DE) analysis in kenaf leaf tissues, three extraction methods (trichloroacetic acid/acetone, urea/thiou... To establish a suitable and effective protocol of protein extraction for two-dimensional gel electrophoresis (2-DE) analysis in kenaf leaf tissues, three extraction methods (trichloroacetic acid/acetone, urea/thiourea, and phenol extraction methods) were applied to the extraction of kenaf leaf protein. The results were compared in regard to protein extraction efficiency, sodiumdodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and 2-DE gels. Furthermore, the 2-DE system was optimized for four aspects: the pH range of IPG (immobilized pH gradient) stripes, sampling methods, sample volumes, and concentration of polyacrylamide gels. The data presented showed that the phenol extraction method is the best method to perform 2-DE analysis of kenaf leaf protein. The protein extracted from phenol extraction method reached the purity of (26.40±0.859)%, showed (25.67±1.53) protein bands in one dimension SDS-PAGE gels, and (1 374±54.44) protein spots on 2-DE gels. The research also indicates that kenaf leaf protein spots were distributed mainly within the pH range of 4-8. More clear background with a better distribution effect and many protein spots could be obtained on 2-DE gels under the conditions of active rehydration loading, 24 cm IPG strips (linear pH gradient of 4-7), 1.4 mg samples, and 12% SDS-PAGE gels. 展开更多
关键词 kenaf protein extraction PROTEOME two-dimensional gel electrophoresis
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A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers 被引量:4
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作者 ZHANG Guang-qing QI Jian-min +6 位作者 ZHANG Xiao-chen FNAG Ping-ping SU Jian-guang TAO Ai-fen LAN Tao WU Wei-ren LIU Ai-min 《Agricultural Sciences in China》 CAS CSCD 2011年第9期1346-1353,共8页
Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The objective of this study was to establish a genetic linkage map of kenaf with higher density of molecu... Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semi- wild variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants. The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD) markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between the adjacent markers. 展开更多
关键词 kenaf genetic map SRAP ISSR RAPD
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TECHNOLOGICAL STUDY OF MAGNESIUM BISULFITE KENAF PULP 被引量:1
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作者 HangChen JinghuiZhou 《天津科技大学学报》 CAS 2004年第A01期67-70,共4页
How to produce bisulfite pulp by kenaf mesta is studied in this paper. After the stalk, the phloem, the xylem of kenaf were analyzed and a series of experiments, the result was gotten as follows: TA 5.6%, temperature ... How to produce bisulfite pulp by kenaf mesta is studied in this paper. After the stalk, the phloem, the xylem of kenaf were analyzed and a series of experiments, the result was gotten as follows: TA 5.6%, temperature 160℃,heat preservation time 120min,CA/FA 1.5, liquor ratio 1:5, and the yield of screened pulp 48.3%, kappa number 42.1, viscosity 948.6ml/g, brightness 51.4% ISO. 展开更多
关键词 洋麻纸浆 亚硫酸氢镁 木质部 韧皮部 亮度 粘滞性
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Polymorphism Analysis of Kenaf (Hibiscus Cannabinus L.) Mutants Based on Random Amplified Polymorphic DNAs (RAPDs) 被引量:2
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作者 Estri Laras Arumingtyas Aminatun Munawarti +1 位作者 Serafinah Indriyani Sudjindro 《材料科学与工程(中英文版)》 2010年第2期56-62,共7页
关键词 RAPD标记 随机扩增多态DNA 突变体 多态性分析 红麻 基础 相似性系数 多基因控制
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Vertex angles effects in the energy absorption of axially crushed kenaf fibre-epoxy reinforced elliptical composite cones
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作者 Mohamed Alkateb S.M.Sapuan +2 位作者 Z.Leman M.R.Ishak Mohammad Jawaid 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2018年第4期327-335,共9页
Experimental quasi-static crushing tests were conducted by using a universal testing machine format kenaf-epoxy composite elliptical cones. The work focused on the vertex angle's effects on energy absorption capab... Experimental quasi-static crushing tests were conducted by using a universal testing machine format kenaf-epoxy composite elliptical cones. The work focused on the vertex angle's effects on energy absorption capability; the vertex angles vary from 0° to 24° in 6 increments. The failure modes of the kenaf fibre epoxy composite elliptical cones were observed utilising delegate photos taken during the quasistatic crushing test. Load-deformation curves and deformation histories of typical specimens are presented and discussed. Moreover, the effects of cone vertex angles on the load carrying capacity and the energy absorption capability are also discussed. The results show that the energy absorption abilities significantly influence the ellipticity vertex angle as the load carrying capacity. We concluded that the quasi-static axial crushing behaviour of elliptical mat laminated composite cones is strongly affected by their structural geometry and the specific energy absorbed by the composite elliptical cones with vertex angles of 6°, 12°, 18°, and 24°, which is more than an elliptical cone with the vertex angle of 0°(the elliptical tube) at any given deformation. However, the specific energy absorption for the elliptical composite cone showed a positive correlation, i.e., the more the angle increased, the more energy was absorbed. In this regard, an elliptical composite cone with a 24° angle exhibited the best energy absorption capability. 展开更多
关键词 kenaf fibres Composite materials Elliptical cone VERTEX angle Collapsible Energy absorption
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红麻种质资源对根结线虫抗性鉴定与评价 被引量:1
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作者 王会芳 曾向萍 +4 位作者 戴志刚 吴智 杨泽茂 陈绵才 粟建光 《植物遗传资源学报》 CAS CSCD 北大核心 2023年第2期514-522,共9页
为鉴定我国红麻种质资源对根结线虫病的抗病性,本研究采用SCAR引物对侵染红麻的根结线虫种群进行了鉴定,并采用田间自然发病及盆栽接种鉴定的的方法,对220份红麻野生或栽培种质资源的根结线虫抗性进行了鉴定与评价。研究结果表明,侵染... 为鉴定我国红麻种质资源对根结线虫病的抗病性,本研究采用SCAR引物对侵染红麻的根结线虫种群进行了鉴定,并采用田间自然发病及盆栽接种鉴定的的方法,对220份红麻野生或栽培种质资源的根结线虫抗性进行了鉴定与评价。研究结果表明,侵染红麻的线虫种类为南方根结线虫(Meloidogyne incognita)。田间抗性鉴定结果显示高抗红麻资源有133份,占资源总数量的60.45%,玫瑰茄、红叶木槿和金钱吊芙蓉全部表现为高抗,高抗比率最高。盆栽接种鉴定结果显示免疫资源有7份,占资源总数的3.18%;高抗资源有98份,占资源总数的44.55%;玫瑰茄均鉴定为高抗等级。红麻种质在田间自然发病和盆栽发病抗性鉴定存在一定的差异,两种方法鉴定结果一致的种质资源共有98份,其中高抗资源69份,占交集资源数量的70.41%;高抗资源中有49份来源于栽培红麻,10份来源于野生近缘,其余来源于玫瑰茄、玫瑰麻和红叶木槿,1份高感品种来自于野生近缘。本研究结果为红麻种质资源发掘利用提供科学依据。 展开更多
关键词 红麻 根结线虫 抗性鉴定
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Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines
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作者 Latifah Saiful Yazan Napsiah Abd Rahman +3 位作者 Kim Wei Chan Wan Nor Hafiza Wan Abd Ghani Yin Sim Tor Jhi Biau Foo 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2016年第5期404-409,共6页
Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-... Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction.Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer(MCF-7 and MDA-MB-231), colon cancer(HT29), lung cancer(A549), cervical cancer(He La) and normal mouse fibroblast(3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope.Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction(P < 0.05). The IC50 of n-butanol fraction for HT29 and MCF-7 was(780.00 ± 28.28)and(895.00 ± 7.07) mg/m L, respectively. Cell shrinkage, membrane blebbing and formation of apoptotic bodies were noted following treatment of HT29 cells with n-butanol fraction.Conclusions: In conclusion, n-butanol fraction was more cytotoxic than crude ethanolic extract and aqueous fraction towards the selected cancerous cell lines and induced apoptosis in HT29 cells. 展开更多
关键词 Defatted kenaf seed meal Phenolic-saponin RICH FRACTION Cytotoxic Apoptosis
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STUDY ON WET STRENGTH PERFORMANCE OF KENAF MULCH
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作者 JinghuiZhou XingeWu HongweiZhu 《天津科技大学学报》 CAS 2004年第A01期374-380,共7页
Optimum applied technical conditions of wet strength agent for kenaf mulch were studied in this article. Breaking length, wet-dry strength ratio, tear index and burst index of kenaf mulch were measured and optimum wet... Optimum applied technical conditions of wet strength agent for kenaf mulch were studied in this article. Breaking length, wet-dry strength ratio, tear index and burst index of kenaf mulch were measured and optimum wet strength agent was selected. The aim is to make mulch have properties of heat preservation, humidity preservation, growth prompting, biodegradation and maximum wet strength and to improve impact resistance of mulch to rainwater so as to assure growth of plant and replace plastic film. 展开更多
关键词 洋麻覆盖物 生物降解 湿强剂 农用薄膜 最优实用技术 树脂
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Mechanism of Kenaf Retting Using Aerobes
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作者 卢士森 陈季华 黄秀宝 《Journal of Donghua University(English Edition)》 EI CAS 2001年第1期55-58,共4页
The experimental results showed that the duration of microbial retting processing of kenaf fibers by using aerobic microbe was four times shorter than that by using anaerobic microbe. The residual gum percentage,break... The experimental results showed that the duration of microbial retting processing of kenaf fibers by using aerobic microbe was four times shorter than that by using anaerobic microbe. The residual gum percentage,breaking strength, breaking elongation and linear density of aerobic retted kenaf bundle fibers did not show significantly difference with that of anaerobic retted kenaf bundle fibers by ANOVA-Tukey's studentized test at a = 5% except for the softness. The bioenergetic principle and the calculation of the amount of ATP produced during the decomposition processing of kenaf gums were used to explain why the retting duration in the case of using aerobic microbes was much shorter than that of using anaerobic microbes. 展开更多
关键词 kenaf fiber MICROBIAL retting aerobe anaerobe bioenergetics properties of fiber.
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Identification of DNA Photolyase Gene of a Drought Tolerance Branching Line of Kenaf (<i>Hibiscus cannabinus</i>L.)
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作者 Estri Laras Arumingtyas Nur Basuki +1 位作者 Sutiman Bambang Sumitro Sudjindro   《American Journal of Plant Sciences》 2012年第6期810-815,共6页
DNA photolyase is a photoreactivation enzyme which has a role in the mechanism of DNA repair. The existence of DNA photolyase gene could affect the success of mutation process. SM026H is a drought tolerance branching ... DNA photolyase is a photoreactivation enzyme which has a role in the mechanism of DNA repair. The existence of DNA photolyase gene could affect the success of mutation process. SM026H is a drought tolerance branching line of kenaf identified by Research Institute for Tobacco and Fibre Crops, Karangploso, Malang, Indonesia which has a potential as a source for mutation breeding. In this research, cloning and sequencing of the DNA photolyase gene of line SM026H were conducted. The DNA of SM026H leaves was isolated using DNeasy Kit, then was amplified by Polymerase Chain Reaction (PCR) using AC1 as a forward primer, paired with AC3R or AC4R as reverse primers and checked using electrophoresis on a 0.7% agarose gel. The AC1-AC3R primer pair produced a DNA fragment with a size of 750 bp, whereas the AC1-AC4R primer pair produced a DNA fragment of 1000 bp in size. Cloning of the AC1-AC3R and AC1-AC4R fragments was done prior to sequencing. Preparation for cloning was done by running and extracting the PCR products from a 0.7% Low Melting Agarose (LMA), and then ligating them to a pCR21 plasmid using an electroporation method. Two sub-fragments of each AC1-AC3R and AC1-AC4R fragments were identified. They were 600 bp and 500 bp, resulting from the AC1-AC3R fragment, and 1300 bp and 500 bp, resulting from the AC1-AC4R fragment. The sequencing result of those sub-fragments was analyzed using a Basic Local Alignment Search Tool (BLAST) program. It was shown that the sequences have a degree of homology to DNA photolyase sequence of Arabidopsis thaliana A. thaliana, Cucumis sativus, Spinacia oleracea, Stellaria longipes and Oryza sativa. These suggested that the kenaf line SM0026H possesses the DNA photolyase gene. However, further study needs to be done to ascertain that the gene was not the cryptochrome. 展开更多
关键词 DNA PHOTOLYASE kenaf Detection
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Isolation and Characterization of Cellulose Whiskers from Kenaf (<i>Hibiscus cannabinus</i>L.) Bast Fibers
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作者 Lukmanul Hakim Zaini Mehdi Jonoobi +1 位作者 Paridah Md. Tahir Samaneh Karimi 《Journal of Biomaterials and Nanobiotechnology》 2013年第1期37-44,共8页
Cellulose whiskers were isolated from kenaf (Hibiscus cannabinus L.) bast fibers via sulfuric acid and hydrochloric acid hydrolysis. Raw kenaf bast, NaOH treated, bleached fibers, sulfuric acid whiskers (SAW) and hydr... Cellulose whiskers were isolated from kenaf (Hibiscus cannabinus L.) bast fibers via sulfuric acid and hydrochloric acid hydrolysis. Raw kenaf bast, NaOH treated, bleached fibers, sulfuric acid whiskers (SAW) and hydrochloric acid whiskers (HClW) morphology, functional groups, crystallinity, and thermal stability were characterized. The TEM images showed that the sulfuric acid and HCl whiskers have average diameters and length range of 3 nm and 100 - 500 nm, respectively. The FTIR study indicated that during the conversion process, most of the hemicellulose and almost all the lignin were removed by the NaOH and subsequent bleaching treatments. The crystallinity of HCl whiskers was found to be higher (84%) than that of sulfuric acid whiskers (72%). Thermogravimetric analysis indicated that HCl whiskers had better thermal stability than the sulfuric acid ones. In addition, a two-stage decomposition behavior was revealed by TGA in the sulfuric acid whiskers because of incorporation of the sulfate group with the cellulose crystals. 展开更多
关键词 Cellulose WHISKERS kenaf Bast Fibers CRYSTALLINITY
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Optimisation of Crude Oil Adsorbent Developed from a Modified Styrene Kenaf Shive
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作者 Zwahruddeen Muhammad Salisu Ishiaku S. Umaru +2 位作者 Danladi Abdullahi Mohammed Kabir Yakubu Diya’uddeen Bashir Hasan 《Journal of Materials Science and Chemical Engineering》 2019年第2期38-51,共14页
A new kind of hydrophobic crude oil sorbent was successfully developed by grafting kenaf shive with styrene monomer. In this paper, crude oil sorbents were developed through regeneration method by varying the initiato... A new kind of hydrophobic crude oil sorbent was successfully developed by grafting kenaf shive with styrene monomer. In this paper, crude oil sorbents were developed through regeneration method by varying the initiator concentration, monomer ratio and particle sizes in the range of 0.5 - 2.5 wt%, 0.5 - 2.0 wt % and 125 - 1000 μm respectively. This research approach for the development of crude oil sorbent from graft modified kenaf shive/core and the aforementioned variable optimization has not been reported. The effect studies of the individual and combine factors were carried out using a statistical experimental design matrix using five-level central composite design (CCD). Respond surface methodology (RSM) was used to optimise and develop equations of the aforementioned variables (initiator concentration, monomer ratio and particle sizes). The optimal swelling capacity of 616% and 267% lower retention were achieved at initiator concentration, monomer ratio and particle size of 12.5 wt%, 1.50 wt% and 562 μm respectively. TGA-DTA, XRD and BET analysis were carried out on optimised sample and, FTIR was carried on both unmodified (UG) and optimized sorbent. Further computations were done for grafting efficiency (GE), homopolymers and density. The findings display the effect of the three variables and navigation equations were generated for further investigations on them. However, the results show the feasibility and robustness of facile oil sorption. 展开更多
关键词 SORBENT INITIATOR CRUDE Oil kenaf Shive Respond Surface Methodology (RSM)
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Effects of Particle Size, Stem Component and Genotype on Absorbency of Kenaf (Hibiscus Cannabinus L.) Grown in Nigeria for Oil-Spill Clean-Up
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作者 Morufat Oloruntoyin Balogun AbdulGaniy Olayinka Raji 《Agricultural Sciences》 2016年第9期621-629,共9页
The efficacies of different products made from different parts of the stem of different varieties of kenaf grown in Nigeria as oil absorbents were tested. Particle sizes, genotypes and whether mixed or sole stem core ... The efficacies of different products made from different parts of the stem of different varieties of kenaf grown in Nigeria as oil absorbents were tested. Particle sizes, genotypes and whether mixed or sole stem core or bast differed among the treatments. The fibre component sank in water, and so was not buoyant;the core was partially buoyant while the mixed fibre and core was completely buoyant. Sorption capacity was the highest in the ground whole stem (8.16 g oil/g sorbent), which also retained (6.31 g oil/g sorbent) the highest quantity of oil. Sorption and retention of oil were the lowest in the fibre (1.66 and 0.69 g oil/g sorbent, respectively). After the first use, only the ground stem can be used to re-absorb up to 1.97 g oil/g sorbent of used sorbent. A mixture of genotypes was more absorbent than a single genotype. The implications of these findings in absorbency research are discussed. 展开更多
关键词 kenaf ABSORBENT Fibre GENOTYPE Bast Sorption
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