The QTL qTGW3-1 was located on chromosome 3 of rice （Oryza sativa L.） and associated with the 1 000-grain weight （TGW） according to the result of our earlier study. With the objective of fine mapping of this locus...The QTL qTGW3-1 was located on chromosome 3 of rice （Oryza sativa L.） and associated with the 1 000-grain weight （TGW） according to the result of our earlier study. With the objective of fine mapping of this locus, we developed a F2 population consisting of 3 428 plants derived from the cross between TGW-related near isogenic line DL017 （BC3F4 generation of GSL 156×Nipponbare） and the recurrent parent Nipponbare. Using six microsatellites, this QTL was delimited between RM5477 and RM6417. Markers MM 1455 and MM 1456 within this region were used for further mapping of this QTL. Finally, qTGW3-1 was fine-mapped into a 89-kb interval between RM5477 and MM1456, which locates in the BAC clone AC107226 harboring five putative candidate genes.展开更多
The purpose of this study is to reveal the effects of historic climate change on rice yield over the middle and lower reaches of the Yangtze River, China, and to better adapt to climate change in the future. This stud...The purpose of this study is to reveal the effects of historic climate change on rice yield over the middle and lower reaches of the Yangtze River, China, and to better adapt to climate change in the future. This study presents the relation of temperature and precipitation and rice components from 1981 to 2003 at 48 early rice stations and 30 middle rice stations. It focuses on an analysis of three stages： flowering, pre-milk, and late milk. The results show that mean maximum temperature and mean daily precipitation at the stages of flowering and pre-milk are most related to early rice yield. Yield change of middle rice is mainly because of mean precipitation change at the flowering stage. Furthermore, percentage of undeveloped grain increases as mean maximum temperature rises at the flowering stage. Over-precipitation in the reproductive stage is a major reason for reduction in yield of early rice. Consecutive rainfall and continuous high temperature can have negative effects on middle rice yield. Global warming would affect middle rice more seriously than early rice.展开更多
Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single...Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett＇s test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.展开更多
基金supported by the National Basic Research Program of China (2010CB129504)the National Key Technologies R&D Program of China (2009BADA2B01)the 948 Project of MOA, China (2011-G2B)
文摘The QTL qTGW3-1 was located on chromosome 3 of rice （Oryza sativa L.） and associated with the 1 000-grain weight （TGW） according to the result of our earlier study. With the objective of fine mapping of this locus, we developed a F2 population consisting of 3 428 plants derived from the cross between TGW-related near isogenic line DL017 （BC3F4 generation of GSL 156×Nipponbare） and the recurrent parent Nipponbare. Using six microsatellites, this QTL was delimited between RM5477 and RM6417. Markers MM 1455 and MM 1456 within this region were used for further mapping of this QTL. Finally, qTGW3-1 was fine-mapped into a 89-kb interval between RM5477 and MM1456, which locates in the BAC clone AC107226 harboring five putative candidate genes.
文摘The purpose of this study is to reveal the effects of historic climate change on rice yield over the middle and lower reaches of the Yangtze River, China, and to better adapt to climate change in the future. This study presents the relation of temperature and precipitation and rice components from 1981 to 2003 at 48 early rice stations and 30 middle rice stations. It focuses on an analysis of three stages： flowering, pre-milk, and late milk. The results show that mean maximum temperature and mean daily precipitation at the stages of flowering and pre-milk are most related to early rice yield. Yield change of middle rice is mainly because of mean precipitation change at the flowering stage. Furthermore, percentage of undeveloped grain increases as mean maximum temperature rises at the flowering stage. Over-precipitation in the reproductive stage is a major reason for reduction in yield of early rice. Consecutive rainfall and continuous high temperature can have negative effects on middle rice yield. Global warming would affect middle rice more seriously than early rice.
基金Supported by National Natural Science Foundation of China(31101131)National Key Technology Research and Development Program(2011BAD16B03)+1 种基金Agricultural Science Independent Innovation Foundation of Jiangsu Province[CX(12)1003]Key Technology Research and Development Program of Jiangsu Province(BE2012309)~~
文摘Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett＇s test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.
