The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good vis...The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good visual appearance of fresh Z. latifolia, inhibited browning, mildew and weight loss at the bottom of Z. latifolia, and there is no significant changes on L, a*, b* and ΔE during the whole storage period. In addition, 1-MCP treatment inhibited the respiratory intensity of Z. latifolia during the first three days of storage, but it was significantly higher than that of the control on the sixth day of storage. Compared with the control, 1-MCP treatment maintained relatively high SOD, CAT, APX activities and low PAL, POD, PLD, lipase and LOX activities, delayed the decline of AsA content, reduced the accumulation of O<sub>2</sub><sup>-</sup>, H<sub>2</sub>O<sub>2</sub> and MDA, and ultimately maintained the integrity of cell structure and delayed the senescence of Z. latifolia. In addition, positive effects of 1-MCP on maintaining the cell structure integrity were observed in this investigation throughout the storage period at 25˚C.展开更多
To investigate the roles of Harvista(a sprayable 1-methylcyclopropene,1-MCP,available component is 150 g·hm^(-2))and Ethephon(1 mL·L^(-1))applied at preharvest in apple(Malus domestica Borkh.'Golden Deli...To investigate the roles of Harvista(a sprayable 1-methylcyclopropene,1-MCP,available component is 150 g·hm^(-2))and Ethephon(1 mL·L^(-1))applied at preharvest in apple(Malus domestica Borkh.'Golden Delicious')fruit cuticular wax biosynthesis,the expression of genes related to fruit cuticular wax biosynthesis and ethylene biosynthesis and signaling,ethylene production rate,respiration rate,wax constituents and structure,and fruit quality were determined at harvest and during cold storage.The results showed that 1-MCP inhibited the expression levels of ethylene biosynthesis and signaling-related genes,decreased fruit ethylene production,and inhibited the expression of Md LACS1,Md CER6,Md CER4 and Md WSD1,which resulted in decreases in alcohols,acids,and esters content in fruit cuticular wax.1-MCP also reduced fruit dropping rate from 17.17%to 12%;maintained fruit firmness,soluble solids,titratable acidity during cold storage;showed about as one fifth in the total length and one third in the widest width of wax crack as that in control at harvest.In contrast,Ethephon produced the opposite effects.In conclusion,1-MCP inhibited fruit ethylene biosynthesis and signaling,and influenced fruit cuticular wax biosynthesis.Thus,the fruit cuticular wax constituents and structure was altered,and the fruit quality were maintained at harvest and during cold storage.The results provide a new technology for improving apple fruit harvest and postharvest quality by preharvest application of sprayable 1-MCP.展开更多
Floral senescence or petal abscission that determine cut flower quality and longevity are primarily caused by the regulation of endogenous ethylene and perception of endogenous and exogenous ethylene in floral organs(...Floral senescence or petal abscission that determine cut flower quality and longevity are primarily caused by the regulation of endogenous ethylene and perception of endogenous and exogenous ethylene in floral organs(such as the petals and gynoecium),in ethylene-sensitive species.Several attempts have been made to prolong flower quality and longevity using silver ions and other chemicals that inhibit ethylene production and perception in floral organs.Among the available compounds,1-methylcyclopropene(1-MCP)has been increasingly used as ethylene-action/perception inhibitor because of its ability to inhibit ethylene action/perception and it is environmentally safe.Although 1-MCP has been used in several cut flower crops for about 25 years,there has been no review yet that critically emphasizes the benefits or difficulties of using this compound for the prolongation of cut flower quality and longevity.Here,we outline the role of 1-MCP in inhibiting ethylene action/perception and petal senescence in cut flowers through the blocking of receptor binding sites,and we discuss how various factors affecting efficacy of 1-MCP(such as concentration,treatment time and temperature,genotype,and flower stage)are involved in the achievement of flower longevity.Moreover,we highlight the advantages of applying nonvolatile and liquid 1-MCP formulation types,as opposed to using the conventional 1-MCP treatment(powder formulation type).We expect that this review will provide useful information for the future utilization of 1-MCP for the maintenance of cut flower longevity.展开更多
The cotton (Gossypium hirsutum L.) crop experiences high temperatures during flowering and boll development, but information regarding the impact of ethylene inhibition and high temperature on early boll development i...The cotton (Gossypium hirsutum L.) crop experiences high temperatures during flowering and boll development, but information regarding the impact of ethylene inhibition and high temperature on early boll development is limited. The objective of this study was to determine the effects of high temperature and the anti-ethylene compound 1-methylcy-cloprone (1-MCP) on G. hirsutum boll development. Treatments consisted of temperature regime (38/20?C and 30/ 20?C), 1-MCP treatment, and days past anthesis (DPA). High temperature decreased ethylene synthesis by 61% at 2 DPA, and 1-MCP caused a 40% decrease in ethylene production at 1 DPA. Glutathione reductase activity increased under high temperature, whereas superoxide dismutase activity (SOD) and membrane peroxidation (malondialdehyde content) remained unchanged. 1-MCP treatment did not affect GR activity in developing bolls. High temperature and 1-MCP treatment increased the weight of cotton bolls collected 8 DPA with an increase of 0.7 and 1 g, respectively. We propose that increased GR activity in bolls exposed to high temperature may mitigate oxidative damage. Additionally, we conclude that ethylene inhibition (either high temperature or 1-MCP-induced) immediately after flowering (1 or 2 DPA) could potentially have positive impacts on early boll growth.展开更多
In this study,integrative metabolomics and transcriptomics analyses were conducted to investigate the effects of 1-methylcyclopropene(1-MCP)on apple fruit quality during long-term cold storage.The results showed that ...In this study,integrative metabolomics and transcriptomics analyses were conducted to investigate the effects of 1-methylcyclopropene(1-MCP)on apple fruit quality during long-term cold storage.The results showed that 1-MCP(1μL/L)treatment could maintain fruits apparent quality(i.e.external color and firmness),inhibit the increase of rot rate and soluble solids content/titratable acidity ratio,decrease ethylene release,and respiratory intensity during cold storage,and extend shelf life.Moreover,1-MCP had long-term effects on the accumulation of many qualities related to metabolite and gene expression in fruits.1-MCP affected genes related to metabolism at the early stage of storage,specifically those of the glycolysis and tricarboxylic acid cycle pathways.Genes related to the degradation of sucrose,starch,and cellulose were inhibited,and some starch and cellulose synthesis genes were up-regulated by 1-MCP.Apart from ethylene synthesis and signal transduction being inhibited by 1-MCP,several enzymes(pectinesterase,pectate lyase,polygalacturonase)were involved in pectin degradation,and degradation products of the cell wall(i.e.D-galacturonic acid and D-glucuronic acid)were also strongly inhibited,further maintaining fruit firmness.Cysteine,as precursor glutathione(GSH)related to plant resistance,up-regulated the synthase gene.However,the expression of genes related to cyanoalanine syn-thase and amino acid utilization pathways was suppressed by 1-MCP Collectively,1-MCP could maintain the postharvest quality of apple fruits.展开更多
Objectives:The aim of this study is to determine the mechanism through which 1-methylcyclopropene(1-MCP)affects the quality of red'Fuji'apples,which were stored for a short duration.Materials and Methods:Red‘...Objectives:The aim of this study is to determine the mechanism through which 1-methylcyclopropene(1-MCP)affects the quality of red'Fuji'apples,which were stored for a short duration.Materials and Methods:Red‘Fuji’apples were treated with 1-MCP(1.0μl/L),stored at 25℃for0,12,24,48,72,and 96 h,and ethylene pro-duction was measured.An integrated metabolomic and transcriptomic analysis was performed on apples stored for 24 h.Results:The release of ethylene was significantly delayed from red‘Fuji’apples subjected to 1-MCP treatment.By performing an integrated transcriptome and metabolome analyses,we identified 117 differentially expressed genes(DEGs)and 44 differentially accumulated metabol-ites(DAMs).By performing functional enrichment analysis,we found that DEGs were involved in the following pathways;carbon metabolism(LPD2,gpmA,LTA2,ACC,PSAT1,MdCAS2),phytohormone signal transduction(EBF 7),amino acid metabolism(MdACS-1),fatty acid metab-olism(LOX1.5,KCS4,KAST),energy metabolism(Lhcb1,Lhcb6,PsbY,GPDHC1,PUMP5I,metabolic pathways(TRE1,HEXO1)and cell wall metabolism(CSLG2).Thus,these DEGs were involved in the ripening of fruits,and they controlled the quality of fruits at the post-harvest stage.The metabolites were enriched with DAMs.These were found to be individually involved in the metabolic pathway,secondary metabolite bio-synthesis,phenylpropanoid biosynthesis,flavonoids,and flavonol synthesis.Conclusions:The results indicate that 1-MCP inhibits the biosynthesis of ethylene and suppresses energy metabolism.Moreover,it also downregulates metabolic pathways and the enzymatic genes related to fruit quality.Therefore,1-MCP delays the ripening of fruits at the post-harvest stage.This study helps us understand how 1-MCP treatment affects the ripening and quality of fruits.展开更多
Objectives:The aim of this work was to analyse the effects of 1-methylcyclopropene(1-MCP)treatment on the metabolisms of membrane lipids in postharvest Anxi persimmons during storage.Materials and methods:Anxi persimm...Objectives:The aim of this work was to analyse the effects of 1-methylcyclopropene(1-MCP)treatment on the metabolisms of membrane lipids in postharvest Anxi persimmons during storage.Materials and methods:Anxi persimmon(Diospyros kaki L.f.cv.Anxi)fruits were treated by paper containing 1-MCP with a concentration of 1.35μl/l.The cellular membrane permeability was analysed by the electric conductivity meter.The activities of lipoxygenase(LOX),phospholipase(PLD)and lipase were determined by spectrophotometry.The component and relative amounts of membrane fatty acids were determined using gas chromatograph(GC).Results:The 1-MCP-treated Anxi persimmons manifested a lower electrolyte leakage rate,lower LOX,PLD and lipase activities,higher levels of unsaturated fatty acids(USFAs),higher ratio of USFAs to saturated fatty acids(SFAs)(U/S),higher index of USFAs(IUFA),but lower levels of SFAs.Conclusions:The degradation and the metabolisms of membrane lipids could be suppressed by 1-MCP treatment,which might be accountable for the delaying softening of postharvest Anxi persimmons during storage.展开更多
Objectives:The purpose of this work was to evaluate the potential application of papers containing 1-methylcyclopropene(1-MCP)postharvest treatment for suppressing fruit decay of fresh Anxi persimmons and its possible...Objectives:The purpose of this work was to evaluate the potential application of papers containing 1-methylcyclopropene(1-MCP)postharvest treatment for suppressing fruit decay of fresh Anxi persimmons and its possible mechanism.Materials and methods:Anxi persimmon fruit were treated with papers containing 1-MCP at the dosage of 1.35μL/L and stored at(25±1)℃and 85 per cent relative humidity for 35 days.During storage,the fruit decay rate and lignin content were evaluated,and the content of total phenolics,the activities of phenylalanine ammonia lyase(PAL),polyphenol oxidase(PPO),peroxidase(POD),chitinase(CHI),and p-1,3-glucanase(GLU)were determined by spectrophotometry.Results:The 1-MCP-treated persimmons displayed a lower fruit decay rate,but higher contents of lignin and total phenolics,higher activities of PAL,PPO,POD,CHI,and GLU.Conclusions:The treatment with 1-MCP could inhibit the fruit decay of postharvest Anxi persimmons,which might be because 1-MCP enhanced fruit disease resistance by increasing the activities of disease resistance-associated enzymes and retaining higher contents of disease resistance-related substances in postharvest fresh Anxi persimmons.These findings indicate that papers containing 1-MCP at the dosage of 1.35μL/L have potential application in suppressing fruit decay and extending storage life of postharvest fresh Anxi persimmons.展开更多
Kiwifruit(Actinidia deliciosa)‘Bruno’is prone to accumulate ethanol rapidly after respiratory climacteric during storage at ambient conditions without stresses,which causes quality deterioration of the fruit associa...Kiwifruit(Actinidia deliciosa)‘Bruno’is prone to accumulate ethanol rapidly after respiratory climacteric during storage at ambient conditions without stresses,which causes quality deterioration of the fruit associated with alcohol off-flavor.For maintaining the postharvest quality of kiwifruit‘Bruno’,the effects of 1.0μL•L^−11-methylcyclopropene(1-MCP)treatment on regulating the ethanol fermentation andγ-aminobutyric acid(GABA)shunt pathway associated with control of alcohol off-flavor were investigated during storage at room temperature(24±1)°C for 27 days.The results showed that 1-MCP treatment significantly reduced the respiration rate,ethylene production,decay rate,ascorbic acid(AsA)loss,and delayed the decline in the firmness and titratable acidity(TA),and the increase in total soluble solid(TSS)in kiwifruit.Furthermore,1-MCP treatment effectively inhibited the increases in contents of acetaldehyde,ethanol,and GABA along with the suppressed activities of key enzymes involved in ethanol fermentation and GABA shunt pathway,such as pyruvate decarboxylase(PDC),alcohol dehydrogenase(ADH),glutamate decarboxylase(GAD),and GABA-transaminase(GABA-T)in kiwifruit during storage.In conclusion,1-MCP treatment efficiently regulated the ethanol fermentation and GABA shunt pathway by delaying the ripening process to avoid the alcohol off-flavor development,thereby contributed to maintaining the quality of the kiwifruit.展开更多
Ethylene is an endogenous plant hormone that increases under adverse environmental conditions, resulting in leaf and fruit abscission and ultimately yield reduction. In cotton, however, the effects of water-deficit st...Ethylene is an endogenous plant hormone that increases under adverse environmental conditions, resulting in leaf and fruit abscission and ultimately yield reduction. In cotton, however, the effects of water-deficit stress on ethylene production have been uncertain. In this study it was hypothesized that application of an ethylene inhibitor 1-Methylcyclo- propene (1-MCP) would prevent ethylene production and result in alleviation of water-deficit stress consequences on the physiology and metabolism of the cotton flower and subtending leaf. To test this hypothesis, growth chamber experiments were conducted in 2009-2010 with treatments consisting of (C) untreated well-watered control, (C + 1MCP) well-watered plus 1-MCP, (WS) untreated water-stressed control, and (WS + 1MCP) water-stressed plus 1-MCP. The plants were subjected to two consecutive drying cycles during flowering, approximately 8 weeks after planting, and 1-MCP was foliar applied at a rate of 10g. ai/ha at the beginning of each drying cycle. The results showed that 1-MCP application had no significant effect on gas exchange functions and did not prevent reductions from water stress in leaf photosynthesis, respiration and stomatal conductance. However, application of 1-MCP resulted in a decrease in sucrose content of water-stressed pistils compared to the control indicating that 1-MCP has the potential to interfere in carbohydrate metabolism of reproductive units.展开更多
文摘The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good visual appearance of fresh Z. latifolia, inhibited browning, mildew and weight loss at the bottom of Z. latifolia, and there is no significant changes on L, a*, b* and ΔE during the whole storage period. In addition, 1-MCP treatment inhibited the respiratory intensity of Z. latifolia during the first three days of storage, but it was significantly higher than that of the control on the sixth day of storage. Compared with the control, 1-MCP treatment maintained relatively high SOD, CAT, APX activities and low PAL, POD, PLD, lipase and LOX activities, delayed the decline of AsA content, reduced the accumulation of O<sub>2</sub><sup>-</sup>, H<sub>2</sub>O<sub>2</sub> and MDA, and ultimately maintained the integrity of cell structure and delayed the senescence of Z. latifolia. In addition, positive effects of 1-MCP on maintaining the cell structure integrity were observed in this investigation throughout the storage period at 25˚C.
基金supported by the National Natural Science Foundation of China(Grant No.31772024)。
文摘To investigate the roles of Harvista(a sprayable 1-methylcyclopropene,1-MCP,available component is 150 g·hm^(-2))and Ethephon(1 mL·L^(-1))applied at preharvest in apple(Malus domestica Borkh.'Golden Delicious')fruit cuticular wax biosynthesis,the expression of genes related to fruit cuticular wax biosynthesis and ethylene biosynthesis and signaling,ethylene production rate,respiration rate,wax constituents and structure,and fruit quality were determined at harvest and during cold storage.The results showed that 1-MCP inhibited the expression levels of ethylene biosynthesis and signaling-related genes,decreased fruit ethylene production,and inhibited the expression of Md LACS1,Md CER6,Md CER4 and Md WSD1,which resulted in decreases in alcohols,acids,and esters content in fruit cuticular wax.1-MCP also reduced fruit dropping rate from 17.17%to 12%;maintained fruit firmness,soluble solids,titratable acidity during cold storage;showed about as one fifth in the total length and one third in the widest width of wax crack as that in control at harvest.In contrast,Ethephon produced the opposite effects.In conclusion,1-MCP inhibited fruit ethylene biosynthesis and signaling,and influenced fruit cuticular wax biosynthesis.Thus,the fruit cuticular wax constituents and structure was altered,and the fruit quality were maintained at harvest and during cold storage.The results provide a new technology for improving apple fruit harvest and postharvest quality by preharvest application of sprayable 1-MCP.
基金supported by the National Research Foundation of Korea (NRF)grant funded by the Korea government (MSIT) (No.2021R1A2C2008951)。
文摘Floral senescence or petal abscission that determine cut flower quality and longevity are primarily caused by the regulation of endogenous ethylene and perception of endogenous and exogenous ethylene in floral organs(such as the petals and gynoecium),in ethylene-sensitive species.Several attempts have been made to prolong flower quality and longevity using silver ions and other chemicals that inhibit ethylene production and perception in floral organs.Among the available compounds,1-methylcyclopropene(1-MCP)has been increasingly used as ethylene-action/perception inhibitor because of its ability to inhibit ethylene action/perception and it is environmentally safe.Although 1-MCP has been used in several cut flower crops for about 25 years,there has been no review yet that critically emphasizes the benefits or difficulties of using this compound for the prolongation of cut flower quality and longevity.Here,we outline the role of 1-MCP in inhibiting ethylene action/perception and petal senescence in cut flowers through the blocking of receptor binding sites,and we discuss how various factors affecting efficacy of 1-MCP(such as concentration,treatment time and temperature,genotype,and flower stage)are involved in the achievement of flower longevity.Moreover,we highlight the advantages of applying nonvolatile and liquid 1-MCP formulation types,as opposed to using the conventional 1-MCP treatment(powder formulation type).We expect that this review will provide useful information for the future utilization of 1-MCP for the maintenance of cut flower longevity.
文摘The cotton (Gossypium hirsutum L.) crop experiences high temperatures during flowering and boll development, but information regarding the impact of ethylene inhibition and high temperature on early boll development is limited. The objective of this study was to determine the effects of high temperature and the anti-ethylene compound 1-methylcy-cloprone (1-MCP) on G. hirsutum boll development. Treatments consisted of temperature regime (38/20?C and 30/ 20?C), 1-MCP treatment, and days past anthesis (DPA). High temperature decreased ethylene synthesis by 61% at 2 DPA, and 1-MCP caused a 40% decrease in ethylene production at 1 DPA. Glutathione reductase activity increased under high temperature, whereas superoxide dismutase activity (SOD) and membrane peroxidation (malondialdehyde content) remained unchanged. 1-MCP treatment did not affect GR activity in developing bolls. High temperature and 1-MCP treatment increased the weight of cotton bolls collected 8 DPA with an increase of 0.7 and 1 g, respectively. We propose that increased GR activity in bolls exposed to high temperature may mitigate oxidative damage. Additionally, we conclude that ethylene inhibition (either high temperature or 1-MCP-induced) immediately after flowering (1 or 2 DPA) could potentially have positive impacts on early boll growth.
基金the National Key Research and Development Program of China(2016YFD0400901)the 2115 Talent Development Program of China Agricultural University(1061-00109019).
文摘In this study,integrative metabolomics and transcriptomics analyses were conducted to investigate the effects of 1-methylcyclopropene(1-MCP)on apple fruit quality during long-term cold storage.The results showed that 1-MCP(1μL/L)treatment could maintain fruits apparent quality(i.e.external color and firmness),inhibit the increase of rot rate and soluble solids content/titratable acidity ratio,decrease ethylene release,and respiratory intensity during cold storage,and extend shelf life.Moreover,1-MCP had long-term effects on the accumulation of many qualities related to metabolite and gene expression in fruits.1-MCP affected genes related to metabolism at the early stage of storage,specifically those of the glycolysis and tricarboxylic acid cycle pathways.Genes related to the degradation of sucrose,starch,and cellulose were inhibited,and some starch and cellulose synthesis genes were up-regulated by 1-MCP.Apart from ethylene synthesis and signal transduction being inhibited by 1-MCP,several enzymes(pectinesterase,pectate lyase,polygalacturonase)were involved in pectin degradation,and degradation products of the cell wall(i.e.D-galacturonic acid and D-glucuronic acid)were also strongly inhibited,further maintaining fruit firmness.Cysteine,as precursor glutathione(GSH)related to plant resistance,up-regulated the synthase gene.However,the expression of genes related to cyanoalanine syn-thase and amino acid utilization pathways was suppressed by 1-MCP Collectively,1-MCP could maintain the postharvest quality of apple fruits.
基金the National Key Research and Development Program of China(2017YFD0401304).
文摘Objectives:The aim of this study is to determine the mechanism through which 1-methylcyclopropene(1-MCP)affects the quality of red'Fuji'apples,which were stored for a short duration.Materials and Methods:Red‘Fuji’apples were treated with 1-MCP(1.0μl/L),stored at 25℃for0,12,24,48,72,and 96 h,and ethylene pro-duction was measured.An integrated metabolomic and transcriptomic analysis was performed on apples stored for 24 h.Results:The release of ethylene was significantly delayed from red‘Fuji’apples subjected to 1-MCP treatment.By performing an integrated transcriptome and metabolome analyses,we identified 117 differentially expressed genes(DEGs)and 44 differentially accumulated metabol-ites(DAMs).By performing functional enrichment analysis,we found that DEGs were involved in the following pathways;carbon metabolism(LPD2,gpmA,LTA2,ACC,PSAT1,MdCAS2),phytohormone signal transduction(EBF 7),amino acid metabolism(MdACS-1),fatty acid metab-olism(LOX1.5,KCS4,KAST),energy metabolism(Lhcb1,Lhcb6,PsbY,GPDHC1,PUMP5I,metabolic pathways(TRE1,HEXO1)and cell wall metabolism(CSLG2).Thus,these DEGs were involved in the ripening of fruits,and they controlled the quality of fruits at the post-harvest stage.The metabolites were enriched with DAMs.These were found to be individually involved in the metabolic pathway,secondary metabolite bio-synthesis,phenylpropanoid biosynthesis,flavonoids,and flavonol synthesis.Conclusions:The results indicate that 1-MCP inhibits the biosynthesis of ethylene and suppresses energy metabolism.Moreover,it also downregulates metabolic pathways and the enzymatic genes related to fruit quality.Therefore,1-MCP delays the ripening of fruits at the post-harvest stage.This study helps us understand how 1-MCP treatment affects the ripening and quality of fruits.
基金This study was supported by the Project of Finance Department of Fujian Province in China(KLe16H01A,KLe16002A)the Science and Technology Innovation Foundation at Fujian Agriculture and Forestry University of China(CXZX2016086,KF2015051).
文摘Objectives:The aim of this work was to analyse the effects of 1-methylcyclopropene(1-MCP)treatment on the metabolisms of membrane lipids in postharvest Anxi persimmons during storage.Materials and methods:Anxi persimmon(Diospyros kaki L.f.cv.Anxi)fruits were treated by paper containing 1-MCP with a concentration of 1.35μl/l.The cellular membrane permeability was analysed by the electric conductivity meter.The activities of lipoxygenase(LOX),phospholipase(PLD)and lipase were determined by spectrophotometry.The component and relative amounts of membrane fatty acids were determined using gas chromatograph(GC).Results:The 1-MCP-treated Anxi persimmons manifested a lower electrolyte leakage rate,lower LOX,PLD and lipase activities,higher levels of unsaturated fatty acids(USFAs),higher ratio of USFAs to saturated fatty acids(SFAs)(U/S),higher index of USFAs(IUFA),but lower levels of SFAs.Conclusions:The degradation and the metabolisms of membrane lipids could be suppressed by 1-MCP treatment,which might be accountable for the delaying softening of postharvest Anxi persimmons during storage.
基金funded by the Science and Technology Project of Finance Department of Fujian Province,China(KLe16002A,KLe16H01A).
文摘Objectives:The purpose of this work was to evaluate the potential application of papers containing 1-methylcyclopropene(1-MCP)postharvest treatment for suppressing fruit decay of fresh Anxi persimmons and its possible mechanism.Materials and methods:Anxi persimmon fruit were treated with papers containing 1-MCP at the dosage of 1.35μL/L and stored at(25±1)℃and 85 per cent relative humidity for 35 days.During storage,the fruit decay rate and lignin content were evaluated,and the content of total phenolics,the activities of phenylalanine ammonia lyase(PAL),polyphenol oxidase(PPO),peroxidase(POD),chitinase(CHI),and p-1,3-glucanase(GLU)were determined by spectrophotometry.Results:The 1-MCP-treated persimmons displayed a lower fruit decay rate,but higher contents of lignin and total phenolics,higher activities of PAL,PPO,POD,CHI,and GLU.Conclusions:The treatment with 1-MCP could inhibit the fruit decay of postharvest Anxi persimmons,which might be because 1-MCP enhanced fruit disease resistance by increasing the activities of disease resistance-associated enzymes and retaining higher contents of disease resistance-related substances in postharvest fresh Anxi persimmons.These findings indicate that papers containing 1-MCP at the dosage of 1.35μL/L have potential application in suppressing fruit decay and extending storage life of postharvest fresh Anxi persimmons.
基金The research was supported by Natural Science Foundation of Zhejiang Province for youth(Grant No.LQ19C200004)the National Natural Science Foundation(Grant No.31671908)the Science and Technology Planning Project of Linhai City(Grant No.2018SHJH04).
文摘Kiwifruit(Actinidia deliciosa)‘Bruno’is prone to accumulate ethanol rapidly after respiratory climacteric during storage at ambient conditions without stresses,which causes quality deterioration of the fruit associated with alcohol off-flavor.For maintaining the postharvest quality of kiwifruit‘Bruno’,the effects of 1.0μL•L^−11-methylcyclopropene(1-MCP)treatment on regulating the ethanol fermentation andγ-aminobutyric acid(GABA)shunt pathway associated with control of alcohol off-flavor were investigated during storage at room temperature(24±1)°C for 27 days.The results showed that 1-MCP treatment significantly reduced the respiration rate,ethylene production,decay rate,ascorbic acid(AsA)loss,and delayed the decline in the firmness and titratable acidity(TA),and the increase in total soluble solid(TSS)in kiwifruit.Furthermore,1-MCP treatment effectively inhibited the increases in contents of acetaldehyde,ethanol,and GABA along with the suppressed activities of key enzymes involved in ethanol fermentation and GABA shunt pathway,such as pyruvate decarboxylase(PDC),alcohol dehydrogenase(ADH),glutamate decarboxylase(GAD),and GABA-transaminase(GABA-T)in kiwifruit during storage.In conclusion,1-MCP treatment efficiently regulated the ethanol fermentation and GABA shunt pathway by delaying the ripening process to avoid the alcohol off-flavor development,thereby contributed to maintaining the quality of the kiwifruit.
文摘Ethylene is an endogenous plant hormone that increases under adverse environmental conditions, resulting in leaf and fruit abscission and ultimately yield reduction. In cotton, however, the effects of water-deficit stress on ethylene production have been uncertain. In this study it was hypothesized that application of an ethylene inhibitor 1-Methylcyclo- propene (1-MCP) would prevent ethylene production and result in alleviation of water-deficit stress consequences on the physiology and metabolism of the cotton flower and subtending leaf. To test this hypothesis, growth chamber experiments were conducted in 2009-2010 with treatments consisting of (C) untreated well-watered control, (C + 1MCP) well-watered plus 1-MCP, (WS) untreated water-stressed control, and (WS + 1MCP) water-stressed plus 1-MCP. The plants were subjected to two consecutive drying cycles during flowering, approximately 8 weeks after planting, and 1-MCP was foliar applied at a rate of 10g. ai/ha at the beginning of each drying cycle. The results showed that 1-MCP application had no significant effect on gas exchange functions and did not prevent reductions from water stress in leaf photosynthesis, respiration and stomatal conductance. However, application of 1-MCP resulted in a decrease in sucrose content of water-stressed pistils compared to the control indicating that 1-MCP has the potential to interfere in carbohydrate metabolism of reproductive units.
