To study the effects of 17beta-estradiol (E2) and progesterone (P4) levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor (Borderdale) and recipient ewes (Inner Mongoli...To study the effects of 17beta-estradiol (E2) and progesterone (P4) levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor (Borderdale) and recipient ewes (Inner Mongolian sheep) were determined during multiple ovulation and embryo transfer (MOET). The results showed that the E2 level increased from the day of initiation by CIDR (Controlled internal drug release devices) treatment and decreased to 3.18±1.32 pg/ml on the day of superovulatory estrus. On the day of embryo collection, the ewes having higher levels of estrogenic peak showed better superovulatory response than the other groups having comparatively lower levels. On the day of initiation of superovulatory treatment, the ewes having lower levels of P4 showed better superovulatory response than those having higher levels of P4. The E2 level of the early abortion group increased from Day 0 to Day 45, while that of two pregnant recipient groups came down from Day 0 to Day 30. The serum P4 level of the early abortion group was significantly lower than that of the pregnant recipient groups on Day 45 (P < 0.05), but no significant difference was found between the two pregnant groups after Day 30. Therefore, the levels of E2 and P4 on definite time affect superovulatory response of donors and conception rate of recipients during MOET.展开更多
BACKGROUND: Recent researches have indicated that estrogen has extensive neuroprotective effects. So some studies designed ovariectomized animal models and administrated with estrogen, so as to verify its neuroprotect...BACKGROUND: Recent researches have indicated that estrogen has extensive neuroprotective effects. So some studies designed ovariectomized animal models and administrated with estrogen, so as to verify its neuroprotective effects. OBJECTIVE: To observe the effect of 17 beta-estradiol on the content of norepinephrine (NE) and level of tumor necrosis factor (TNF) in submandibular glands of rats with sympathetic nerve injury, and analyze the dose-dependence and pathway of action. DESIGN: A randomized control animal study.SETTINGS: Department of Hand Surgery, the 252 Hospital of Chinese PLA; Department of Hand Surgery, Union Hospital affiliated to Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: Fifty healthy female Wistar rats were randomly divided into 5 groups with 10 rats in each group: sham-operated group, ovariectomy+6-OHDA+saline group, ovariectomy+6-OHDA+17β-estradiol 50, 200 and 500 μg/kg groups. METHODS: The experiments were carried out in Tongji Medical College, Huazhong University of Science and Technology between October 2005 and March 2006. Bilateral ovaries were only exposed but not resected for the rats in the sham-operated group, but bilateral ovaries were resected in all the other groups. In the ovariectomy+6-OHDA+17β-estradiol 50, 200 and 500 μg/kg groups, the rats were administrated with intraperitoneal injection of 6-OHDA (8 mg/kg), and then immediately given 17β-estradiol of corresponding dosages respectively, once a day for 10 days continuously. Rats in the sham-operated group and ovariectomy+6-OHDA+saline group were administrated with saline of the same volume. After administration, 5 rats in each group were killed to determine the NE contents in bilateral submandibular glands with high performance liquid chromatography-electrochemical detector (HPLC-ECD), and the other 5 rats were used to determine the TNF levels in submandibular glands with enzyme-linked immunosorbant assay.MAIN OUTCOME MEASURES: The NE contents and TNF levels in submandibular glands of rats in each group were observed. RESULTS: All the 50 rats were involved in the analysis of results. ① The NE content was obviously lower in the ovariectomy+6-OHDA+saline group than in the sham-operated group [(1 035±196), (1 823±314) ng/g, P < 0.05], there were no significant differences between the ovariectomy+6-OHDA+17β-estradiol 50 μg/kg group and ovariectomy+6-OHDA+saline group [(1 004±253), (1 035±196) ng/g, P > 0.05], but obviously higher in the ovariectomy+6-OHDA+17β-estradiol 200 and 500 μg/kg groups than in the ovariectomy+6-OHDA+saline group [(1 487±268), (1 939±274), (1 035±196) ng/g, P < 0.05]. ② The TNF level was obviously higher in the ovariectomy+6-OHDA+saline group than in the sham-operated group [(3.498±0.792), (1.893±0.533) ng/g, P < 0.05], there were no significant differences between the ovariectomy+ 6-OHDA+17β-estradiol 50 μg/kg group and ovariectomy+6-OHDA+saline group [(3.328±0.712), (3.498±0.792) ng/g, P > 0.05], but obviously lower in the ovariectomy+6-OHDA+17β-estradiol 200 and 500 μg/kg groups than in the ovariectomy+6-OHDA+saline group [(2.639±0.438), (2.016±0.619), (3.498±0.792) ng/g, P < 0.05]. CONCLUSION: Estrogen has obvious protective effect dose-dependently on 6-OHDA induced chemical sympathetic nerve terminal injury in rats, and it may play its protective role by reducing TNF level and ameliorating inflammatory reaction.展开更多
目的研究17β-雌二醇(E2)对蛛网膜下腔出血(SAH)后迟发型脑血管痉挛(DCV)的抑制作用。方法雄性Wistar大鼠50只随机分为5组:①空白组,②假穿刺组,③SAH组,④SAH+E2组,⑤SAH+安慰剂组。采用酶联免疫吸附法(ELISA)检测血浆中内皮素-1(ET-1...目的研究17β-雌二醇(E2)对蛛网膜下腔出血(SAH)后迟发型脑血管痉挛(DCV)的抑制作用。方法雄性Wistar大鼠50只随机分为5组:①空白组,②假穿刺组,③SAH组,④SAH+E2组,⑤SAH+安慰剂组。采用酶联免疫吸附法(ELISA)检测血浆中内皮素-1(ET-1)含量,末端脱氧核苷酸转移酶介导的生物素脱氧尿嘧啶核苷酸缺口末端标记法(TUNEL)检测颞叶神经元凋亡情况,通过测定基底动脉血管横截面积判断脑血管痉挛情况。结果实验结果显示SAH后7 d SAH+E2组基底动脉横截面积与SAH组和SAH+安慰剂组相比明显变大(P<0.01);与SAH组和SAH+安慰剂组相比,SAH+E2组血浆ET-1浓度明显减少(P<0.01);TUNEL染色显示SAH+E2组颞叶皮质神经元凋亡程度较SAH组和SAH+安慰剂组显著性减轻。结论持续给予E2维持其生理浓度可以有效预防SAH后迟发型脑血管痉挛,部分可能与E2可以抑制ET-1的产生有关。展开更多
BACKGROUND: In vitro cultures of neural stem cells have shown that estrogen can regulate beta-amyloid precursor protein (β-APP) metabolism and reduce amyloid-beta production. OBJECTIVE: To investigate the effects of ...BACKGROUND: In vitro cultures of neural stem cells have shown that estrogen can regulate beta-amyloid precursor protein (β-APP) metabolism and reduce amyloid-beta production. OBJECTIVE: To investigate the effects of long-term oral administration of compound nylestriol or low-dose 17beta-estradiol on β-APP and mRNA expression in the hippocampus of ovariectomized (OVX) rats. DESIGN, TIME AND SETTING: This randomized and controlled experiment was performed at the Animal Laboratory and Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University between April 2003 and May 2004. MATERIALS: According to body mass, 50 six-month-old female Sprague-Dawley rats were randomly divided into five groups (n = 10 per group): normal control, sham operation, OVX model, 17beta-estradiol (Sigma, USA), and compound nylestriol tablet (Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University) groups. METHODS: Rats in OVX plus 17beta-estradiol and OVX plus compound nylestriol tablet groups underwent ovariectomy. On the second day after surgery, rats were intragastrically given 17beta-estradiol (100 μg/kg), once per day or compound nylestriol tablet (0.5 mg/kg) and levonorgestrel (0.15 mg/kg) every 2 days. MAIN OUTCOME MEASURES: β-APP expression in the hippocampus of OVX rats was determined using immunohistochemistry (SABC method) and β-APP mRNA expression was analyzed by in situ hybridization. The results were quantitatively analyzed using cell counting and average optical density. RESULTS: The number and optical density of β-APP-positive neurons in every subregion of the hippocampus of OVX rats was dramatically increased compared with normal and sham operation groups following 35 weeks of administration (P < 0.05). Levels of β-APP were decreased following oral administration of compound nylestriol or 17beta-estradiol. In situ hybridization showed that long-term estrogen deficiency and oral administration of compound nylestriol or 17beta-estradiol did not alter the number of β-APP mRNA-positive neurons. CONCLUSION: The results show that long-term estrogen deficiency results in an increase of expression of β-APP though no changes in the expression of β-APP mRNA are detected. Replacement of estrogen with low-dose 17 beta-estradiol or compound nylestriol tablet inhibits the expression of β-APP in the hippocampus to the same extent.展开更多
In this work,Fe3O4-Al2O3@CNFs nanocomposite was synthesised and used as a nanosorbent in the ultrasound-assisted dispersive magnetic solid phase extraction(UA-DMSPE)of 17-beta estradiol(E2)in wastewater samples.The qu...In this work,Fe3O4-Al2O3@CNFs nanocomposite was synthesised and used as a nanosorbent in the ultrasound-assisted dispersive magnetic solid phase extraction(UA-DMSPE)of 17-beta estradiol(E2)in wastewater samples.The quantification of E2 was achieved using high performance liquid chromatography coupled with diode array detector(HPLC-DAD).Various parameters affecting the efficiency of this sample preparation technique were optimised to achieve excellent sensitivity and high recoveries of E2.Response surface methodology was utilised for optimisation of these parameters.Using the optimised conditions,the linear dynamic range was achieved in the range of 0.1e1000 mgL^-1and the correlation coefficient was found to be 0.9981.The preconcentration factor,enrichment factor,limit of detection(LOD)and limit of quantification(LOQ)were 67,169,0.025 mgL^-1and 0.083 mg L1,respectively.The relative standard deviation(%RSD)for the intraday(n?10)and interday(n?5 working days)were 1.8%and 3.3%,respectively.The developed UA-DMSPE/HPLC-DAD method was applied for the preconcentration and determination of E2 in wastewater samples.The obtained results indicated that E2 was present in the wastewater samples.展开更多
基金Acknowledgment This work was supported by the National Natural Science Foundation of China （No. 30270648 and No. 30500270）, Zhejiang Province Scientific and Technological Project （No. 2005C22052） and Zhejiang Province Science Foundation （No. Y304194）. We thank Dr Ji- Min Zhang of the University of California for linguistic revision of the manuscript.
文摘瞄准：估计外长的 estradiol 是否在小鸡在初发的细菌房间(PGC ) 的移植上有任何效果。方法：使肥沃的卵与 17beta-estradiol 被对待(在阶段 X (白天 0 孵化) 的 E <SUB>2</SUB>)(80 μg/egg ) ，阶段 8-10 (孵化 30 h ) 和 13-15 (孵化 55 h ) 。控制仅仅收到了车辆(乳剂) 。在 PGC 数字的变化根据发展阶段在不同的天被测量。结果：在男正确性腺，然而并非在女左性腺，在阶段 28-30 (孵化 132 h )，在聚集的 PGC 的吝啬的数字的重要减少与控制相比被观察( P 【
0.05 )当在在每个阶段的正确、左的性腺的全部的 PGC 数字没变化时( P 】
0.05 )。结论：现在的学习提供 E2 在离开的男权利，然而并非女性在 PGC 的本地化上有重要效果的证据，在阶段 28-30 的鸡胚胎的性腺，与控制相比。
基金funded by Agricultural Research Institute"948"Program of China(2000-2005)
文摘To study the effects of 17beta-estradiol (E2) and progesterone (P4) levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor (Borderdale) and recipient ewes (Inner Mongolian sheep) were determined during multiple ovulation and embryo transfer (MOET). The results showed that the E2 level increased from the day of initiation by CIDR (Controlled internal drug release devices) treatment and decreased to 3.18±1.32 pg/ml on the day of superovulatory estrus. On the day of embryo collection, the ewes having higher levels of estrogenic peak showed better superovulatory response than the other groups having comparatively lower levels. On the day of initiation of superovulatory treatment, the ewes having lower levels of P4 showed better superovulatory response than those having higher levels of P4. The E2 level of the early abortion group increased from Day 0 to Day 45, while that of two pregnant recipient groups came down from Day 0 to Day 30. The serum P4 level of the early abortion group was significantly lower than that of the pregnant recipient groups on Day 45 (P < 0.05), but no significant difference was found between the two pregnant groups after Day 30. Therefore, the levels of E2 and P4 on definite time affect superovulatory response of donors and conception rate of recipients during MOET.
文摘BACKGROUND: Recent researches have indicated that estrogen has extensive neuroprotective effects. So some studies designed ovariectomized animal models and administrated with estrogen, so as to verify its neuroprotective effects. OBJECTIVE: To observe the effect of 17 beta-estradiol on the content of norepinephrine (NE) and level of tumor necrosis factor (TNF) in submandibular glands of rats with sympathetic nerve injury, and analyze the dose-dependence and pathway of action. DESIGN: A randomized control animal study.SETTINGS: Department of Hand Surgery, the 252 Hospital of Chinese PLA; Department of Hand Surgery, Union Hospital affiliated to Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: Fifty healthy female Wistar rats were randomly divided into 5 groups with 10 rats in each group: sham-operated group, ovariectomy+6-OHDA+saline group, ovariectomy+6-OHDA+17β-estradiol 50, 200 and 500 μg/kg groups. METHODS: The experiments were carried out in Tongji Medical College, Huazhong University of Science and Technology between October 2005 and March 2006. Bilateral ovaries were only exposed but not resected for the rats in the sham-operated group, but bilateral ovaries were resected in all the other groups. In the ovariectomy+6-OHDA+17β-estradiol 50, 200 and 500 μg/kg groups, the rats were administrated with intraperitoneal injection of 6-OHDA (8 mg/kg), and then immediately given 17β-estradiol of corresponding dosages respectively, once a day for 10 days continuously. Rats in the sham-operated group and ovariectomy+6-OHDA+saline group were administrated with saline of the same volume. After administration, 5 rats in each group were killed to determine the NE contents in bilateral submandibular glands with high performance liquid chromatography-electrochemical detector (HPLC-ECD), and the other 5 rats were used to determine the TNF levels in submandibular glands with enzyme-linked immunosorbant assay.MAIN OUTCOME MEASURES: The NE contents and TNF levels in submandibular glands of rats in each group were observed. RESULTS: All the 50 rats were involved in the analysis of results. ① The NE content was obviously lower in the ovariectomy+6-OHDA+saline group than in the sham-operated group [(1 035±196), (1 823±314) ng/g, P < 0.05], there were no significant differences between the ovariectomy+6-OHDA+17β-estradiol 50 μg/kg group and ovariectomy+6-OHDA+saline group [(1 004±253), (1 035±196) ng/g, P > 0.05], but obviously higher in the ovariectomy+6-OHDA+17β-estradiol 200 and 500 μg/kg groups than in the ovariectomy+6-OHDA+saline group [(1 487±268), (1 939±274), (1 035±196) ng/g, P < 0.05]. ② The TNF level was obviously higher in the ovariectomy+6-OHDA+saline group than in the sham-operated group [(3.498±0.792), (1.893±0.533) ng/g, P < 0.05], there were no significant differences between the ovariectomy+ 6-OHDA+17β-estradiol 50 μg/kg group and ovariectomy+6-OHDA+saline group [(3.328±0.712), (3.498±0.792) ng/g, P > 0.05], but obviously lower in the ovariectomy+6-OHDA+17β-estradiol 200 and 500 μg/kg groups than in the ovariectomy+6-OHDA+saline group [(2.639±0.438), (2.016±0.619), (3.498±0.792) ng/g, P < 0.05]. CONCLUSION: Estrogen has obvious protective effect dose-dependently on 6-OHDA induced chemical sympathetic nerve terminal injury in rats, and it may play its protective role by reducing TNF level and ameliorating inflammatory reaction.
文摘目的研究17β-雌二醇(E2)对蛛网膜下腔出血(SAH)后迟发型脑血管痉挛(DCV)的抑制作用。方法雄性Wistar大鼠50只随机分为5组:①空白组,②假穿刺组,③SAH组,④SAH+E2组,⑤SAH+安慰剂组。采用酶联免疫吸附法(ELISA)检测血浆中内皮素-1(ET-1)含量,末端脱氧核苷酸转移酶介导的生物素脱氧尿嘧啶核苷酸缺口末端标记法(TUNEL)检测颞叶神经元凋亡情况,通过测定基底动脉血管横截面积判断脑血管痉挛情况。结果实验结果显示SAH后7 d SAH+E2组基底动脉横截面积与SAH组和SAH+安慰剂组相比明显变大(P<0.01);与SAH组和SAH+安慰剂组相比,SAH+E2组血浆ET-1浓度明显减少(P<0.01);TUNEL染色显示SAH+E2组颞叶皮质神经元凋亡程度较SAH组和SAH+安慰剂组显著性减轻。结论持续给予E2维持其生理浓度可以有效预防SAH后迟发型脑血管痉挛,部分可能与E2可以抑制ET-1的产生有关。
基金the National Key Medical Technologies Research and Development Program of China during the Tenth Five-Year Plan Period, No. 2001BA702B03
文摘BACKGROUND: In vitro cultures of neural stem cells have shown that estrogen can regulate beta-amyloid precursor protein (β-APP) metabolism and reduce amyloid-beta production. OBJECTIVE: To investigate the effects of long-term oral administration of compound nylestriol or low-dose 17beta-estradiol on β-APP and mRNA expression in the hippocampus of ovariectomized (OVX) rats. DESIGN, TIME AND SETTING: This randomized and controlled experiment was performed at the Animal Laboratory and Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University between April 2003 and May 2004. MATERIALS: According to body mass, 50 six-month-old female Sprague-Dawley rats were randomly divided into five groups (n = 10 per group): normal control, sham operation, OVX model, 17beta-estradiol (Sigma, USA), and compound nylestriol tablet (Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University) groups. METHODS: Rats in OVX plus 17beta-estradiol and OVX plus compound nylestriol tablet groups underwent ovariectomy. On the second day after surgery, rats were intragastrically given 17beta-estradiol (100 μg/kg), once per day or compound nylestriol tablet (0.5 mg/kg) and levonorgestrel (0.15 mg/kg) every 2 days. MAIN OUTCOME MEASURES: β-APP expression in the hippocampus of OVX rats was determined using immunohistochemistry (SABC method) and β-APP mRNA expression was analyzed by in situ hybridization. The results were quantitatively analyzed using cell counting and average optical density. RESULTS: The number and optical density of β-APP-positive neurons in every subregion of the hippocampus of OVX rats was dramatically increased compared with normal and sham operation groups following 35 weeks of administration (P < 0.05). Levels of β-APP were decreased following oral administration of compound nylestriol or 17beta-estradiol. In situ hybridization showed that long-term estrogen deficiency and oral administration of compound nylestriol or 17beta-estradiol did not alter the number of β-APP mRNA-positive neurons. CONCLUSION: The results show that long-term estrogen deficiency results in an increase of expression of β-APP though no changes in the expression of β-APP mRNA are detected. Replacement of estrogen with low-dose 17 beta-estradiol or compound nylestriol tablet inhibits the expression of β-APP in the hippocampus to the same extent.
基金the Department of Science and Technology(DST,South Africa)/National Nanoscience Postgraduate Teaching and Training Programme(NNPTTP)and National Research Foundation(NRF,South Africa,grant no.99270&91230).
文摘In this work,Fe3O4-Al2O3@CNFs nanocomposite was synthesised and used as a nanosorbent in the ultrasound-assisted dispersive magnetic solid phase extraction(UA-DMSPE)of 17-beta estradiol(E2)in wastewater samples.The quantification of E2 was achieved using high performance liquid chromatography coupled with diode array detector(HPLC-DAD).Various parameters affecting the efficiency of this sample preparation technique were optimised to achieve excellent sensitivity and high recoveries of E2.Response surface methodology was utilised for optimisation of these parameters.Using the optimised conditions,the linear dynamic range was achieved in the range of 0.1e1000 mgL^-1and the correlation coefficient was found to be 0.9981.The preconcentration factor,enrichment factor,limit of detection(LOD)and limit of quantification(LOQ)were 67,169,0.025 mgL^-1and 0.083 mg L1,respectively.The relative standard deviation(%RSD)for the intraday(n?10)and interday(n?5 working days)were 1.8%and 3.3%,respectively.The developed UA-DMSPE/HPLC-DAD method was applied for the preconcentration and determination of E2 in wastewater samples.The obtained results indicated that E2 was present in the wastewater samples.
