Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and...Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and 1,7-dimethylxanthine(17X) and assess the activity of cytochrome P-450 CYP2A6.Methods The contents of five major metabolites of caffeine in the urine were determined by RP-HPLC method.Frequency distribution histogram was drawn by calculating the 17U/(AFMU+1X+1U+17X+17U) and then evaluated the activity of CYP2A6.Results The frequency distribution histograms of CYP2A6 approximately indicated three distinct groups,the cut of point is 0.23 between fast metabolizer and intermediate type.And the cut of point is 0.15 between slow metabolizer and intermediate type.Conclusion The method is simple and rapid,suitable for the determination of metabolites of caffeine in urine.The method can be used to assay the activity of CYP2A6.展开更多
目的运用系统回顾和Meta分析的方法评价CYP2A6基因多态性与S-1化疗获益的关系。方法检索Pubmed、CNKI、CBM和万方中文数据库,按照纳入和排除标准筛选关于CYP2A6基因与S-1化疗获益相关的病例对照研究或队列研究,检索时限为数据库建库至2...目的运用系统回顾和Meta分析的方法评价CYP2A6基因多态性与S-1化疗获益的关系。方法检索Pubmed、CNKI、CBM和万方中文数据库,按照纳入和排除标准筛选关于CYP2A6基因与S-1化疗获益相关的病例对照研究或队列研究,检索时限为数据库建库至2014年6月。使用Rev Man 5.3和Stata 11.2进行统计分析。结果共纳入7篇文献进入分析,文献质量评价结果显示纳入研究质量良好。一般资料结果显示,在东方人种中,CYP2A6＊1/＊1（W/W）频率平均为25.6%;CYP2A6＊4A,＊7,＊9,＊10存在一种突变（W/V）频率平均为48.8%,CYP2A6＊4A,＊7,＊9,＊10存在2种突变（V/V）频率平均为25.6%。Meta分析发现：1突变型V/V与野生型W/W及突变型W/V患者的S-1化疗敏感性差异有统计学意义,合并优势比（OR）为0.27（95%CI：0.14~0.51）;2野生型W/W与突变型W/V及V/V生存HR差异有统计学意义,合并HR值为1.71（95%CI：0.99~2.94）。结论 CYP2A6＊4A,＊7,＊9,＊10与晚期肿瘤患者S-1化疗获益存在一定关联性。但因为纳入研究的数量有限,需要有大样本、多变量的分析研究来进一步证实和完善本研究结果。展开更多
文摘Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and 1,7-dimethylxanthine(17X) and assess the activity of cytochrome P-450 CYP2A6.Methods The contents of five major metabolites of caffeine in the urine were determined by RP-HPLC method.Frequency distribution histogram was drawn by calculating the 17U/(AFMU+1X+1U+17X+17U) and then evaluated the activity of CYP2A6.Results The frequency distribution histograms of CYP2A6 approximately indicated three distinct groups,the cut of point is 0.23 between fast metabolizer and intermediate type.And the cut of point is 0.15 between slow metabolizer and intermediate type.Conclusion The method is simple and rapid,suitable for the determination of metabolites of caffeine in urine.The method can be used to assay the activity of CYP2A6.
文摘目的运用系统回顾和Meta分析的方法评价CYP2A6基因多态性与S-1化疗获益的关系。方法检索Pubmed、CNKI、CBM和万方中文数据库,按照纳入和排除标准筛选关于CYP2A6基因与S-1化疗获益相关的病例对照研究或队列研究,检索时限为数据库建库至2014年6月。使用Rev Man 5.3和Stata 11.2进行统计分析。结果共纳入7篇文献进入分析,文献质量评价结果显示纳入研究质量良好。一般资料结果显示,在东方人种中,CYP2A6＊1/＊1（W/W）频率平均为25.6%;CYP2A6＊4A,＊7,＊9,＊10存在一种突变（W/V）频率平均为48.8%,CYP2A6＊4A,＊7,＊9,＊10存在2种突变（V/V）频率平均为25.6%。Meta分析发现：1突变型V/V与野生型W/W及突变型W/V患者的S-1化疗敏感性差异有统计学意义,合并优势比（OR）为0.27（95%CI：0.14~0.51）;2野生型W/W与突变型W/V及V/V生存HR差异有统计学意义,合并HR值为1.71（95%CI：0.99~2.94）。结论 CYP2A6＊4A,＊7,＊9,＊10与晚期肿瘤患者S-1化疗获益存在一定关联性。但因为纳入研究的数量有限,需要有大样本、多变量的分析研究来进一步证实和完善本研究结果。
