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RNA-Seq profiling of circular RNAs in human colorectal cancer 5-fluorouracil resistance and potential biomarkers
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作者 Pei-Qiu Cheng Yu-Jing Liu +5 位作者 Sheng-An Zhang Lu Lu Wen-Jun Zhou Dan Hu Han-Chen Xu Guang Ji 《World Journal of Gastrointestinal Oncology》 SCIE 2022年第3期678-689,共12页
BACKGROUND Colorectal cancer(CRC)is a commonly diagnosed cancer of the digestive system worldwide.Although chemotherapeutic agents and targeted therapeutic drugs are currently available for CRC treatment,drug resistan... BACKGROUND Colorectal cancer(CRC)is a commonly diagnosed cancer of the digestive system worldwide.Although chemotherapeutic agents and targeted therapeutic drugs are currently available for CRC treatment,drug resistance is a problem that cannot be ignored and needs to be solved.AIM To explore the relationship between circular RNA(circRNA)and CRC drug resistance.circRNA plays a key role in the occurrence and development of cancers,but its function in the process of drug resistance has not been widely revealed.METHODS To explore the role of circRNA in 5-fluorouracil(5-Fu)resistance,we performed the circRNA expression profile in two CRC cell lines and their homologous 5-Fu resistant cells by high-throughput sequencing.RESULTS We validated the differentially expressed circRNAs in other two paired CRC cells,confirmed that circ_0002813 and circ_0000236 could have a potential competitive endogenous RNA mechanism and be involved in the formation of 5-Fu resistance.And we combined the sequencing results of mRNA to construct the regulatory network of circRNA-miRNA-mRNA.CONCLUSION Our study revealed that circ_0002813 and circ_0000236 may as the biomarkers to predict the occurrence of 5-Fu resistance in CRC. 展开更多
关键词 Colorectal cancer 5-fluorouracil resistance Circular RNAs RNA sequencing Network prediction Biomarkers
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Breast Cancer Resistance Protein Expression and 5-Fluorouracil Resistance 被引量:5
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作者 JIAN-HUI YUAN JIN-QUAN CHENG +7 位作者 LONG-YUAN JIANG WEI-DONG JI LIANG-FENG GUO JIAN-JUN LIU XING-YUN XU JING-SONG HE XIAN-MING WANG ZHI-XIONG ZHUANG 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2008年第4期290-295,共6页
Objective To filtrate breast cancer resistance protein (BCRP)-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance. Methods MTT assay was performed to filtra... Objective To filtrate breast cancer resistance protein (BCRP)-mediated resistant agents and to investigate clinical relationship between BCRP expression and drug resistance. Methods MTT assay was performed to filtrate BCRP-mediated resistant agents with BCRP expression cell model and to detect chemosensitivity of breast cancer tissue specimens to these agents. A high performance liquid chromatography (HPLC) assay was established, and was used to measure the relative dose of intracellular retention resistant agents. RT-PCR and immunohistochemistry (IHC) were employed to investigate the BCRP expression in breast cancer tissue specimens. Results MTT assay showed that the expression of BCRP increased with the increasing resistance of 5-fluorouracil (5-Fu) (P〈0.05, n=3) in the cell model, while HPLC assay indicated that the intracellular retention dose of 5-Fu was significantly correlated with the expression of BCRP (t=-0.897, P〈0.05, n=3). A total of 140 breast cancer tissue specimens were collected. BCRP-positive expression was detected in forty-seven specimens by both RT-PCR and IHC. As shown by MTT assay subsequently, the resistance index (RI) of 47 BCRP-positive breast cancer tissue specimens to 5-Fu was 7-12 times as high as that of adjacent normal tissue samples. BCRP expression was related to 5-Fu resistance (R2=0.8124, P〈0.01). Conclusion Resistance to 5-Fu can be mediated by BCRR Clinical chemotherapy for breast cancer patients can be optimized based on BCRP-positive expression. 展开更多
关键词 Breast cancer resistance protein 5-fluorouracil Breast cancer resistance CHEMOTHERAPY
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Recent studies of 5-fluorouracil resistance in pancreatic cancer 被引量:3
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作者 Wei-Bin Wang Yu Yang +3 位作者 Yu-Pei Zhao Tai-Ping Zhang Quan Liao Hong Shu 《World Journal of Gastroenterology》 SCIE CAS 2014年第42期15682-15690,共9页
Resistance to 5-fluorouracil(5-FU), an important anticancer drug, is a serious challenge in the treatment of pancreatic cancer. Equilibrative nucleoside transporter 1 and multidrug-resistance protein(MRP) 5 and MRP8, ... Resistance to 5-fluorouracil(5-FU), an important anticancer drug, is a serious challenge in the treatment of pancreatic cancer. Equilibrative nucleoside transporter 1 and multidrug-resistance protein(MRP) 5 and MRP8, rather than P-glycoprotein, play important roles in 5-FU transport. Thymidylate synthase, dihydropyrimidine dehydrogenase, methylenetetrahydrofolate reductase and thymidine phosphorylase are four key enzymes involved in 5-FU metabolism. Other metabolic enzymes, including uridine monophosphate synthetase, also contribute to chemoresistance. Intracellular signaling pathways are an integrated network, and nuclear factor kappa-light-chain-enhancer of activated B cells, AKT and extracellular signal-regulated kinases are signaling pathways that are particularly relevant to 5-FU resistance. In addition, recent reports indicate that STAT-3 is a crucial survival protein. Proteomic assays provide a powerful tool for identifying target proteins and understanding the role of micro RNAs and stromal factors to facilitate the development of strategies to combat 5-FU resistance. 展开更多
关键词 5-fluorouracil resistance Transporters Metabolic enzyme Signaling pathway Stromal factors MicroRNA Proteomic investigation
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Raddeanin A promotes apoptosis and ameliorates 5-fluorouracil resistance in cholangiocarcinoma cells 被引量:6
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作者 Shuang-Shuang Guo Ying Wang Qing-Xia Fan 《World Journal of Gastroenterology》 SCIE CAS 2019年第26期3380-3391,共12页
BACKGROUND Bile duct cancer is characterized by fast metastasis and invasion and has been regarded as one of the most aggressive tumors due to the absence of effective diagnosis at an early stage.Therefore,it is in th... BACKGROUND Bile duct cancer is characterized by fast metastasis and invasion and has been regarded as one of the most aggressive tumors due to the absence of effective diagnosis at an early stage.Therefore,it is in the urgent demand to explore novel diagnostic approaches and therapeutic strategies for bile duct cancer to improve patient survival.Raddeanin A(RA)is extracted from the anemone raddeana regel and has been demonstrated to play antitumor roles in various cancers.AIM To investigate the effects of RA treatment on bile duct cancer cells.METHODS In this study,four cholangiocarcinoma cell lines(RBE,LIPF155C,LIPF178C,and LICCF)treated with RA were used to test the cell viability.The RA-associated cell functional analysis,5-fluorouracil(5-Fu)effectiveness as well as cell cycle-and apoptosis-related protein expression were investigated.RESULTS RA reduced cell viability in a dose-dependent pattern in four cell lines,and the migration and colony formation abilities were also impaired by RA in RBE and LIPF155C cell lines.RA sensitized cell lines to 5-Fu treatment and enhanced the effects of 5-Fu in cholangiocarcinoma.Also,RA decreased protein expression of Wee1,while the combinational effect of RA and 5-Fu decreased protein expressions of cyclooxygenase-2,B cell lymphoma 2,and Wee1 but increased protein levels of Bax,cyclin D1,and cyclin E.CONCLUSION Taken together,the results suggest that RA acts as an anti-cancer agent and enhancer of 5-Fu in bile duct cancer cells via regulating multiple cell cycle and apoptosis-related proteins.This finding provides novel clues to exploring a novel antitumor drug for bile duct cancer. 展开更多
关键词 BILE DUCT CANCER Raddeanin A 5-fluorouracil Cell CYCLE APOPTOSIS
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Expression analysis of the R2R3-MYB gene family in upland cotton and functional study of GhMYB3D5 in regulating Verticillium wilt resistance
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作者 Jie Liu Zhicheng Wang +15 位作者 Bin Chen Guoning Wang Huifeng Ke Jin Zhang Mengjia Jiao Yan Wang Meixia Xie Yanbin Li Dongmei Zhang Xingyi Wang Qishen Gu Zhengwen Sun Liqiang Wu Xingfen Wang Zhiying Ma Yan Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第10期3294-3310,共17页
Improving plant resistance to Verticillium wilt(VW),which causes massive losses in Gossypium hirsutum,is a global challenge.Crop plants need to efficiently allocate their limited energy resources to maintain a balance... Improving plant resistance to Verticillium wilt(VW),which causes massive losses in Gossypium hirsutum,is a global challenge.Crop plants need to efficiently allocate their limited energy resources to maintain a balance between growth and defense.However,few transcriptional regulators specifically respond to Verticillium dahliae and the underlying mechanism has not been identified in cotton.In this study,we found that the that expression of most R2R3-MYB members in cotton is significantly changed by V.dahliae infection relative to the other MYB types.One novel R2R3-MYB transcription factor(TF)that specifically responds to V.dahliae,GhMYB3D5,was identified.GhMYB3D5 was not expressed in 15 cotton tissues under normal conditions,but it was dramatically induced by V.dahliae stress.We functionally characterized its positive role and underlying mechanism in VW resistance.Upon V.dahliae infection,the up-regulated GhMYB3D5 bound to the GhADH1 promoter and activated GhADH1expression.In addition,GhMYB3D5 physically interacted with GhADH1 and further enhanced the transcriptional activation of GhADH1.Consequently,the transcriptional regulatory module GhMYB3D5-GhADH1 then promoted lignin accumulation by improving the transcriptional levels of genes related to lignin biosynthesis(GhPAL,GhC4H,Gh4CL,and GhPOD/GhLAC)in cotton,thereby enhancing cotton VW resistance.Our results demonstrated that the GhMYB3D5 promotes defense-induced lignin accumulation,which can be regarded as an effective way to orchestrate plant immunity and growth. 展开更多
关键词 COTTON Verticillium wilt resistance GhMYB3D5 GhADH1 transcriptional regulatory module
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Ghrelin regulates insulin resistance by targeting insulin-like growth factor-1 receptor via miR-455-5p in hepatic cells
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作者 GUO Zhan-hong JU Yue-jun +4 位作者 SHEN Ting ZHANG Lin-qi SHENG Zhong-qi WU Run-ze KONG Ying-hong 《Journal of Hainan Medical University》 CAS 2024年第1期22-28,共7页
Objective: To explore the mechanism by which ghrelin regulates insulin sensitivity through modulation of miR-455-5p in hepatic cells. Methods: HepG2 cells were treated with or without DAG (1 μM). Glucose consumption,... Objective: To explore the mechanism by which ghrelin regulates insulin sensitivity through modulation of miR-455-5p in hepatic cells. Methods: HepG2 cells were treated with or without DAG (1 μM). Glucose consumption, intracellular glycogen content, phosphorylation of PI3K and Akt stimulated by insulin, expression of miR-455-5p, as well as IGF-1R protein level were analyzed. In addition, bioinformatic analysis, dual luciferase reporter assay, miR- 455-5p mimic or inhibitor treatment was conducted to investigate the molecular mechanisms. Results: High glucose treatment upregulated miR-455-5p expression but reduced glucose consumption and glycogen content. DAG reversed the effect of high glucose on glucose metabolism, increased protein level of IGF-1R and phosphorylation of PI3K/Akt stimulated by insulin, as well as downregulated miR-455-5p expression. Bioinformatic analysis indicated IGF-1R was the target of miR-455-5p. Dual luciferase reporter assay, as well as transfection with miR-455-5p mimic/inhibitor confirmed that DAG activated IGF-1R/PI3K/Akt signaling via inhibiting miR-455-5p. Conclusion: DAG improves insulin resistance via miR-455-5p- mediated activation of IGF-1R/PI3K/Akt system, suggesting that suppression of miR-455-5p or activation of DAG may be potential targets for T2DM therapy. 展开更多
关键词 GHRELIN miR-455-5p IGF-1R Insulin resistance HepG2 cells
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Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway 被引量:5
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作者 Xiao-long WU Ying CHEN +1 位作者 Wen-cui KONG Zhong-quan ZHAO 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2020年第3期234-245,共12页
Hepatocellular carcinoma(HCC)is a malignant tumor with high morbidity and mortality globally.It accounts for the majority of primary liver cancer cases.Amyloid precursor protein(APP),a cell membrane protein,plays a vi... Hepatocellular carcinoma(HCC)is a malignant tumor with high morbidity and mortality globally.It accounts for the majority of primary liver cancer cases.Amyloid precursor protein(APP),a cell membrane protein,plays a vital role in the pathogenesis of Alzheimer’s disease,and has been found to be implicated in tumor growth and metastasis.Therefore,to understand the relationship between APP and 5-fluorouracil(5-FU)resistance in liver cancer,Cell Counting Kit-8,apoptosis and cell cycle assays,western blotting,and reverse transcription-quantitative polymerase chain reaction(q PCR)analysis were performed.The results demonstrated that APP expression in Bel7402-5-FU cells was significantly up-regulated,as compared with that in Bel7402 cells.Through successful construction of APP-silenced(si APP)and overexpressed(OE)Bel7402 cell lines,data revealed that the Bel7402-APP751-OE cell line was insensitive,while the Bel7402-si APP cell line was sensitive to 5-FU in comparison to the matched control group.Furthermore,APP overexpression decreased,while APP silencing increased 5-FU-induced apoptosis in Bel7402 cells.Mechanistically,APP overexpression and silencing can regulate the mitochondrial apoptotic pathway and the expression of apoptotic suppressor genes(B-cell lymphoma-2(Bcl-2)and B-cell lymphoma-extra large(Bcl-xl)).Taken together,these results preliminarily revealed that APP overexpression contributes to the resistance of liver cancer cells to 5-FU,providing a new perspective for drug resistance. 展开更多
关键词 Amyloid precursor protein 5-fluorouracil resistance Mitochondrial apoptotic pathway Hepatocellular carcinoma
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Novel insights into D-Pinitol based therapies:a link between tau hyperphosphorylation and insulin resistance 被引量:3
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作者 Dina Medina-Vera Antonio Jesús López-Gambero +4 位作者 Juan Antonio Navarro Carlos Sanjuan Elena Baixeras Juan Decara Fernando Rodríguez de Fonseca 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期289-295,共7页
Alzheimer’s disease is a neurodegenerative disorder characterized by the amyloid accumulation in the brains of patients with Alzheimer’s disease.The pathogenesis of Alzheimer’s disease is mainly mediated by the pho... Alzheimer’s disease is a neurodegenerative disorder characterized by the amyloid accumulation in the brains of patients with Alzheimer’s disease.The pathogenesis of Alzheimer’s disease is mainly mediated by the phosphorylation and aggregation of tau protein.Among the multiple causes of tau hyperphosphorylation,brain insulin resistance has generated much attention,and inositols as insulin sensitizers,are currently considered candidates for drug development.The present narrative review revises the interactions between these three elements:Alzheimer’s disease-tau-inositols,which can eventually identify targets for new disease modifiers capable of bringing hope to the millions of people affected by this devastating disease. 展开更多
关键词 Alzheimer’s disease cyclin-dependent kinase 5 diabetes D-PINITOL inositols insulin resistance KINASES PHOSPHORYLATION PI3K/Akt tau
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Multilevel optoelectronic hybrid memory based on N-doped Ge_(2)Sb_(2)Te_(5)film with low resistance drift and ultrafast speed
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作者 吴奔 魏涛 +6 位作者 胡敬 王瑞瑞 刘倩倩 程淼 李宛飞 凌云 刘波 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第10期724-730,共7页
Multilevel phase-change memory is an attractive technology to increase storage capacity and density owing to its high-speed,scalable and non-volatile characteristics.However,the contradiction between thermal stability... Multilevel phase-change memory is an attractive technology to increase storage capacity and density owing to its high-speed,scalable and non-volatile characteristics.However,the contradiction between thermal stability and operation speed is one of key factors to restrain the development of phase-change memory.Here,N-doped Ge_(2)Sb_(2)Te_(5)-based optoelectronic hybrid memory is proposed to simultaneously implement high thermal stability and ultrafast operation speed.The picosecond laser is adopted to write/erase information based on reversible phase transition characteristics whereas the resistance is detected to perform information readout.Results show that when N content is 27.4 at.%,N-doped Ge_(2)Sb_(2)Te_(5)film possesses high ten-year data retention temperature of 175℃and low resistance drift coefficient of 0.00024 at 85℃,0.00170 at 120℃,and 0.00249 at 150℃,respectively,owing to the formation of Ge–N,Sb–N,and Te–N bonds.The SET/RESET operation speeds of the film reach 520 ps/13 ps.In parallel,the reversible switching cycle of the corresponding device is realized with the resistance ratio of three orders of magnitude.Four-level reversible resistance states induced by various crystallization degrees are also obtained together with low resistance drift coefficients.Therefore,the N-doped Ge_(2)Sb_(2)Te_(5)thin film is a promising phase-change material for ultrafast multilevel optoelectronic hybrid storage. 展开更多
关键词 multilevel optoelectronic hybrid memory N-doped Ge_(2)Sb_(2)Te_(5)thin film low resistance drift ultrafast speed
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Curcumin Reverses 5-Fluorouracil Resistance by Promoting Human Colon Cancer HCT-8/5-FU Cell Apoptosis and Down-regulating Heat Shock Protein 27 and P-Glycoprotein 被引量:9
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作者 HE Wen-ting ZHU Yan-hua +6 位作者 ZHANG Tong ABULIMITI Patima ZENG Fan-ye ZHANG Li-ping LUO Ling-juan XIE Xin-mei ZHANG Hong-liang 《Chinese Journal of Integrative Medicine》 SCIE CAS CSCD 2019年第6期416-424,共9页
Objective: To investigate the potential mechanisms that curcumin reverses 5-fluorouracil(5-FU) multidrug resistance(MDR). Methods: Cell growth and the inhibitory rate of curcumin(2–25 μg/mL) and/or5-FU(0.05–1000 μ... Objective: To investigate the potential mechanisms that curcumin reverses 5-fluorouracil(5-FU) multidrug resistance(MDR). Methods: Cell growth and the inhibitory rate of curcumin(2–25 μg/mL) and/or5-FU(0.05–1000 μg/mL) on human colon cancer HCT-8 and HCT-8/5-FU(5-FU-resistant cel line) were determined using cel counting kit-8(CCK-8) assay. Apoptosis and cel cycle after 5-FU and/or curcumin treatment were detected by ?ow cytometry(FCM) and transmission electron microscopy(TEM). The expression of the multidrug resistance related factors p-glycoprotein(P-gp) and heat shock protein 27(HSP-27) genes and proteins were analyzed by reverse transcription polymerase chain reaction(RT-PCR) and Western blotting(WB), respectively. Results: The inhibitory rate of curcumin or 5-FU on HCT-8 and HCT-8/5-FU cells proliferation at exponential phase were in a dosedependent manner, HCT-8 cell line was more sensitive to curcumin or 5-FU when compared the inhibitory rate of HCT-8/5-FU. The 50% inhibitory concentration(IC50) of combination 5-FU and curcumin(4.0 μg/mL)in HCT-8/5-FU was calculated as 179.26 μg/mL, with reversal fold of 1.85. Another IC50 of combination 5-FU and curcumin(5.5 μg/mL) in HCT-8/5-FU was calculated as 89.25 μg/mL, with reversal fold of 3.71. Synergistic effect of 5-FU and curcumin on HCT-8 and HCT-8/5-FU cells were found. The cell cycle analysis performed by FCM showed that HCT-8 and HCT-8/5-FU cel s mostly accumulated at G0/G1 phase, which suggested a synergistic effect of curcumin and 5-FU to induce apoptosis. FCM analysis found that the percentage of apoptosis of cel s treated with curcumin, 5-FU and their combination were signi?cantly increased compared to the control group(P<0.05), and the percentage of apoptosis of the combination groups were slightly higher than other groups(P<0.05). The m RNA levels of P-gp(0.28±0.02) and HSP-27(0.28±0.09) in HCT-8/5-FU cel s treated with combination drugs were lower than cel s treated with 5-FU alone(P-gp, 0.48±0.07, P=0.009;HSP-27, 0.57±0.10, P=0.007). The protein levels of P-gp(0.25±0.06) and HSP-27(0.09±0.02) in HCT-8/5-FU cells treated with combination drugs were decreased when compared to 5-FU alone(P-gp, 0.46±0.02, P=0.005;HSP-27, 0.43±0.01, P=0.000). Conclusions: Curcumin can inhibit the proliferation of human colon cancer cells. Curcumin has the ability of reversal effects on the multidrug resistance of human colon cancer cells lines HCT-8/5-FU. Down-regulation of P-gp and HSP-27 may be the mechanism of curcumin reversing the drug resistance of HCT-8/5-FU to 5-FU. 展开更多
关键词 CURCUMIN Chinese medicine 5-fluorouracil MULTIDRUG resistance HCT-8 HCT-8/5-FU col on cancer P-gp HSP-27
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Study on the Correlation Between SFRP-5 Expression Level, Insulin Resistance, and Glycolipid Metabolism in Gestational Diabetes Mellitus
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作者 Bei Wang Chenyuan Cao +4 位作者 Rui Li Yan An Fang Wang Yuxiang Huang Jinjin Qin 《Journal of Clinical and Nursing Research》 2023年第2期53-58,共6页
Objective:To investigate the correlation between serum secretory frizzled-related protein 5(SFRP-5)expression levels and insulin resistance and glucolipid metabolism in patients with gestational diabetes mellitus(GDM)... Objective:To investigate the correlation between serum secretory frizzled-related protein 5(SFRP-5)expression levels and insulin resistance and glucolipid metabolism in patients with gestational diabetes mellitus(GDM).Methods:Baseline data were collected from 58 patients with GDM and 51 healthy controls who were admitted Affiliated Hospital of Hebei University from May 2020 to June 2022.sSTRA5 concentrations in peripheral blood of pregnant women were measured,and SFRP-5 levels in patients with different GDM types and normal controls were analyzed by logistic regression models.Results:The levels of triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),fasting blood glucose(FBG),fasting insulin(FINS),hemoglobin A1c(HbA1c),and homeostasis model assessment-estimated insulin resistance(HOMA-IR)were higher in the observation group than in the control group,with statistically significant differences(P<0.05),while the expression levels of high-density lipoprotein cholesterol(HDL-C)and serum SFRP-5 were lower than in the control group,with statistically significant differences(P<0.05);serum SFRP-5,TG,TC,FBG,and HOMA-IR were all risk factors for GDM(P<0.05).Conclusion:Elevated serum sSTRA5 may be involved in the regulation of insulin resistance in the body and the regulation of blood glucose in the body by affecting lipid metabolism and inflammatory response. 展开更多
关键词 Gestational diabetes mellitus Secretory frizzled-related protein 5 Insulin resistance Glucolipid metabolism
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2014-2018年江苏省人源喹诺酮耐药1,4,[5],12:i:-沙门氏菌的基因组学初步分析
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作者 郑东宇 马恺 +3 位作者 周翌婧 吴高林 霍翔 乔昕 《中国人兽共患病学报》 CAS CSCD 北大核心 2024年第8期739-744,共6页
目的分析江苏省喹诺酮耐药1,4,[5],12:i:-沙门氏菌分子流行病学特征。方法用cgMLST分析江苏省及欧美来源菌株的分子流行病学特征,并初步分析喹诺酮耐药1,4,[5],12:i:-沙门氏菌可能的传播特征。结果13株喹诺酮耐药1,4,[5],12:i:-沙门氏... 目的分析江苏省喹诺酮耐药1,4,[5],12:i:-沙门氏菌分子流行病学特征。方法用cgMLST分析江苏省及欧美来源菌株的分子流行病学特征,并初步分析喹诺酮耐药1,4,[5],12:i:-沙门氏菌可能的传播特征。结果13株喹诺酮耐药1,4,[5],12:i:-沙门氏菌共注释11大类抗性基因(Antibiotic resistance genes,ARGs)。其中氨基糖苷类ARGs检出率最高(100%);12株喹诺酮耐药菌株(92.3%)均携带IncHI2/IncHI2A质粒类型;不同国家/地区来源菌株质粒介导喹诺酮耐药(PMQR)基因携带分析显示美国及欧洲来源菌株携带6类PMQR基因,qnrB19检出率最高。江苏省来源菌株携带3类PMQR基因类型,aac(6′)-Ib-cr检出率最高(11.84%);不同国家/地区来源菌株cgMLST位点差异分析显示形成3个主要流行谱系。结论江苏省人源喹诺酮耐药1,4,[5],12:i:-沙门氏菌分离株与欧美菌株可能具有共同进化来源,PMQR基因携带水平存在国家/地区差异。 展开更多
关键词 1 4 [5] 12:i:-沙门氏菌 喹诺酮耐药 分子流行病学
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ONECUT2高表达介导胃癌复发患者5-FU化疗耐药
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作者 朱海峰 陈述 +1 位作者 龙卫国 王旭 《江苏大学学报(医学版)》 CAS 2024年第1期19-24,共6页
目的:探究One cut结构域家族成员2(ONECUT2)是否可以作为对5-FU耐药的胃癌复发患者的治疗靶点。方法:利用癌症基因组图谱(TCGA)数据库分析ONECUT2基因在胃癌患者及胃癌复发患者的表达情况。利用基因集富集分析(GSEA)评估TCGA数据库中ONE... 目的:探究One cut结构域家族成员2(ONECUT2)是否可以作为对5-FU耐药的胃癌复发患者的治疗靶点。方法:利用癌症基因组图谱(TCGA)数据库分析ONECUT2基因在胃癌患者及胃癌复发患者的表达情况。利用基因集富集分析(GSEA)评估TCGA数据库中ONECUT2表达量与药物代谢相关生物学过程的相关性。利用CCK-8细胞毒性实验评估胃癌细胞敲低或过表达ONECUT2对5-FU的耐药情况。分析30例胃癌术后复发患者ONECUT2表达量与5-FU治疗效果的相关性。结果:TCGA数据库分析显示,在经受过化疗且复发的胃癌患者中,ONECUT2高表达的患者无病间隔期(disease-free interval,DFI)更短。GSEA分析结果表明,ONECUT2高表达与异生药物代谢通路呈正相关,这提示可能与胃癌患者的耐药相关。体外实验中,敲低ONECUT2降低胃癌细胞对5-FU的半数抑制浓度(IC_(50))值;反之,过表达ONECUT2增加细胞对5-FU的IC_(50)值。30例胃癌复发患者对5-FU的药物敏感性与ONECUT2表达量相关。结论:胃癌复发患者的ONECUT2表达量更高,ONECUT2高表达与胃癌复发患者的5-FU耐药相关,可能是潜在的治疗靶点。 展开更多
关键词 One cut结构域家族成员2 胃癌复发 5-FU耐药 GSEA 细胞毒性 半数抑制浓度
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高产优质抗病玉米新品种达美5号的选育、特性分析及栽培技术
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作者 余长平 周华平 +7 位作者 叶青松 吴承国 肖能武 秦光明 李永学 王致云 陈强 刘永忠 《湖北农业科学》 2024年第9期1-5,41,共6页
玉米(Zea mays L.)新品种达美5号是十堰市农业科学院和华中农业大学以HZ1711为母本、Q4-2为父本育成的杂交种,2020—2021年2年区域试验平均产量为9950.85 kg/hm^(2),比对照品种华玉11增产8.53%,具有突出的高产稳产、抗逆广适、品质优良... 玉米(Zea mays L.)新品种达美5号是十堰市农业科学院和华中农业大学以HZ1711为母本、Q4-2为父本育成的杂交种,2020—2021年2年区域试验平均产量为9950.85 kg/hm^(2),比对照品种华玉11增产8.53%,具有突出的高产稳产、抗逆广适、品质优良、制种产量高的特性,于2022年8月通过湖北省农作物品种审定委员会审定。总结了达美5号的选育过程、特征特性、抗逆性、品质和产量表现,介绍了栽培和制种技术要点,为品种的应用推广提供参考。 展开更多
关键词 玉米(Zea mays L.) 达美5 高产 优质 抗病 选育 特性分析 栽培技术
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妊娠期糖尿病患者血清分泌性卷曲相关蛋白-5、热休克蛋白60、溶质载体家族16成员11的表达及其与胰岛素抵抗的关系
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作者 魏曼 苑文贺 +3 位作者 刘红云 马迪蒙 闫康禄 韩宁 《实用临床医药杂志》 CAS 2024年第20期60-65,共6页
目的探讨分泌性卷曲相关蛋白-5(sFRP5)、热休克蛋白60(HSP60)、溶质载体家族16成员11(SLC16A11)在妊娠期糖尿病(GDM)患者血清中的表达及其与胰岛素抵抗的关系。方法选取2022年1月—2023年12月在本院就诊的120例GDM患者为研究组,另选取同... 目的探讨分泌性卷曲相关蛋白-5(sFRP5)、热休克蛋白60(HSP60)、溶质载体家族16成员11(SLC16A11)在妊娠期糖尿病(GDM)患者血清中的表达及其与胰岛素抵抗的关系。方法选取2022年1月—2023年12月在本院就诊的120例GDM患者为研究组,另选取同期120例健康孕妇为对照组。检测血清sFRP5、HSP60、SLC16A11表达水平;检测并计算胰岛素抵抗相关指标[空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)]的水平;采用Pearson法分析血清sFRP5、HSP60、SLC16A11与胰岛素抵抗的相关性;采用Logistic回归分析法与受试者工作特征(ROC)曲线分析血清sFRP5、HSP60、SLC16A11与GDM的相关性。结果研究组血清sFRP5表达低于对照组,血清HSP60、SLC16A11、FBG、FINS、HOMA-IR高于对照组,差异有统计学意义(P<0.05)。Pearson相关性分析显示,血清sFRP5与HSP60、SLC16A11、FBG、FINS、HOMA-IR呈负相关(P<0.05);血清HSP60与SLC16A11、FBG、FINS、HOMA-IR呈正相关(P<0.05);血清SLC16A11与FBG、FINS、HOMA-IR呈正相关(P<0.05)。多因素Logistic回归分析显示,HSP60、SLC16A11、FBG、FINS、HOMA-IR是妊娠期患者发生GDM的影响因素,sFRP5是保护因素(P<0.05)。血清sFRP5、HSP60、SLC16A11联合诊断妊娠期患者发生GDM的曲线下面积(AUC)为0.924,3项指标联合诊断价值优于各指标单独诊断(P均<0.05)。结论GDM患者血清sFRP5水平降低,HSP60、SLC16A11水平升高;3项指标均为妊娠期患者发生GDM的影响因素,且与胰岛素抵抗相关;3项指标联合对GDM具有较高的诊断效能。 展开更多
关键词 分泌性卷曲相关蛋白-5 热休克蛋白60 溶质载体家族16成员11 妊娠期糖尿病 胰岛素抵抗
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1,3-Bis(2-chloroethyl)-1-nitrosourea enhances the inhibitory effect of Resveratrol on 5-fluorouracil sensitive/resistant colon cancer cells 被引量:4
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作者 Dipon Das Ranjan Preet +2 位作者 Purusottam Mohapatra Shakti Ranjan Satapathy Chanakya Nath Kundu 《World Journal of Gastroenterology》 SCIE CAS 2013年第42期7374-7388,共15页
AIM:To study the mechanism of 5-fluorouracil(5-FU)resistance in colon cancer cells and to develop strategies for overcoming such resistance by combination treatment.METHODS:We established and characterized a 5-FU resi... AIM:To study the mechanism of 5-fluorouracil(5-FU)resistance in colon cancer cells and to develop strategies for overcoming such resistance by combination treatment.METHODS:We established and characterized a 5-FU resistance(5-FU-R)cell line derived from continuous exposure(25μmol/L)to 5-FU for 20 wk in 5-FU sensitive HCT-116 cells.The proliferation and expression of different representative apoptosis and anti-apoptosis markers in 5-FU sensitive and 5-FU resistance cells were measured by the MTT assay and by Western blotting,respectively,after treatment with Resveratrol(Res)and/or 1,3-Bis(2-chloroethyl)-1-nitrosourea(BCNU).Apoptosis and cell cycle arrest was measured by 4',6'-diamidino-2-phenylindole hydrochloride staining and fluorescence-activated cell sorting analysis,respectively.The extent of DNA damage was measured by the Comet assay.We measured the visible changes in the DNA damage/repair cascade by Western blotting.RESULTS:The widely used chemotherapeutic agents BCNU and Res decreased the growth of 5-FU sensitive HCT-116 cells in a dose dependent manner.Combined application of BCNU and Res caused more apoptosis in5-FU sensitive cells in comparison to individual treatment.In addition,the combined application of BCNU and Res caused a significant decrease of major DNA base excision repair components in 5-FU sensitive cells.We established a 5-FU resistance cell line(5-FU-R)from 5-FU-sensitive HCT-116(mismatch repair deficient)cells that was not resistant to other chemotherapeutic agents(e.g.,BCNU,Res)except 5-FU.The 5-FU resistance of 5-FU-R cells was assessed by exposure to increasing concentrations of 5-FU followed by the MTT assay.There was no significant cell death noted in5-FU-R cells in comparison to 5-FU sensitive cells after5-FU treatment.This resistant cell line overexpressed anti-apoptotic[e.g.,AKT,nuclear factorκB,FLICE-like inhibitory protein),DNA repair(e.g.,DNA polymerase beta(POL-β),DNA polymerase eta(POLH),protein Flap endonuclease 1(FEN1),DNA damage-binding protein 2(DDB2)]and 5-FU-resistance proteins(thymidylate synthase)but under expressed pro-apoptotic proteins(e.g.,DAB2,CK1)in comparison to the parental cells.Increased genotoxicity and apoptosis were observed in resistant cells after combined application of BCNU and Res in comparison to untreated or parental cells.BCNU increased the sensitivity to Res of 5-FU resistant cells compared with parental cells.Fifty percent cell death were noted in parental cells when 18μmol/L of Res was associated with fixed concentration(20μmol/L)of BCNU,but a much lower concentration of Res(8μmol/L)was needed to achieve the same effect in 5-FU resistant cells.Interestingly,increased levels of adenomatous polyposis coli and decreased levels POL-β,POLH,FEN1 and DDB2 were noted after the same combined treatment in resistant cells.CONCLUSION:BCNU combined with Res exerts a synergistic effect that may prove useful for the treatment of colon cancer and to overcome drug resistance. 展开更多
关键词 5-fluorouracil 1 3-Bis(2-chloroethyl)-1-nitrosourea RESVERATROL COLON cancer Combination therapy
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Microstructure,microhardness and corrosion resistance of laser cladding Ni−WC coating on AlSi5Cu1Mg alloy 被引量:12
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作者 Min ZENG Hong YAN +1 位作者 Bao-biao YU Zhi HU 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第9期2716-2728,共13页
The microstructure,microhardness,and corrosion resistance of laser cladding Ni−WC coating on the surface of AlSi5Cu1Mg alloy were investigated by scanning electron microscopy,X-ray diffraction,microhardness testing,im... The microstructure,microhardness,and corrosion resistance of laser cladding Ni−WC coating on the surface of AlSi5Cu1Mg alloy were investigated by scanning electron microscopy,X-ray diffraction,microhardness testing,immersion corrosion testing,and electrochemical measurement.The results show that a smooth coating containing NiAl,Ni_(3)Al,M_(7)C_(3),M_(23)C_(6)phases(M=Ni,Al,Cr,W,Fe)and WC particles is prepared by laser cladding.Under a laser scanning speed of 120 mm/min,the microhardness of the cladding coating is 9−11 times that of AlSi5Cu1Mg,due to the synergistic effect of excellent metallurgical bond and newly formed carbides.The Ni−WC coating shows higher corrosion potential(−318.09 mV)and lower corrosion current density(12.33μA/cm^(2))compared with the matrix.The crack-free,dense cladding coating obviously inhibits the penetration of Cl^(−)and H^(+),leading to the remarkedly improved corrosion resistance of cladding coating. 展开更多
关键词 laser cladding Ni−WC coating AlSi5Cu1Mg mechanical properties corrosion resistance
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Effect of Black Plate on Corrosion Resistance of T5 Tinplate 被引量:5
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作者 HUANG Xing-qiao LI Ning JIANG Li-min LI De-yu 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2006年第2期59-64,共6页
The effect of black plate on corrosion resistance of T5 tinplate was studied by glow discharge spectrograph, X-ray diffractometer (XRD), stress tester, roughness tester and metallographic microscope. The result show... The effect of black plate on corrosion resistance of T5 tinplate was studied by glow discharge spectrograph, X-ray diffractometer (XRD), stress tester, roughness tester and metallographic microscope. The result shows that black plate influences corrosion resistance of T5 tinplate intensely. It also indicates that the increase of content of manganese, phosphorus, silicon and aluminum in black plate would reduce the corrosion resistance of T5 tinplate and the increase of degree of crystal orientation on (200) crystal face, |X-Y| value (internal stress difference within two orientation), roughness and exposure degree of iron grain after the same acid wash of black plate would enhance the corrosion resistance of T5 tinplate and the grain number of black plate has small effect on corrosion resistance of T5 tinplate. 展开更多
关键词 T5 tinplate black plate COMPONENT crystal orientation internal stress corrosion resistance
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抗逆大豆IND-ØØ41Ø-5转化体特异性定性PCR检测方法的建立及其标准化
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作者 肖芳 李允静 +5 位作者 武玉花 李俊 高鸿飞 翟杉杉 吴刚 梁晋刚 《中国油料作物学报》 CAS CSCD 北大核心 2024年第5期1148-1156,共9页
抗逆大豆IND-ØØ41Ø-5已经批准获得我国进口用作加工原料的安全证书,含有外源HaHB4基因和bar标记基因。本研究根据抗逆大豆IND-ØØ41Ø-5转化体5′端和3′端插入位点旁侧序列信息,设计19对引物,结合罗萨里... 抗逆大豆IND-ØØ41Ø-5已经批准获得我国进口用作加工原料的安全证书,含有外源HaHB4基因和bar标记基因。本研究根据抗逆大豆IND-ØØ41Ø-5转化体5′端和3′端插入位点旁侧序列信息,设计19对引物,结合罗萨里奥农业生物技术学院公司的1对引物,针对20对引物组合利用定性PCR技术进行引物特异性筛选,结果显示5′端的14号引物特异性良好,优化后获得最佳反应体系和反应程序;经测试,该引物特异性好、稳定性高,检出限达0.1%,扩增片段大小248 bp。经8家有资质实验室对本方法的特异性、检出限、重复性和再现性进行验证,各项参数均达到国家标准要求。本研究成功建立了抗逆大豆IND-ØØ41Ø-5转化体定性PCR检测方法,为IND-ØØ41Ø-5转化体在国内的安全监管提供技术支撑。 展开更多
关键词 转基因 抗逆大豆IND-ØØ41Ø-5 转化体特异性 定性PCR
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Study on the mechanism of action of Feng-Liao-Chang-Wei-Kang combined with 5-fluorouracil in the treatment of colitis-associated colon cancer
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作者 WANG Qian-ru ZHONG Li-fan HUANG Ling 《Journal of Hainan Medical University》 CAS 2023年第18期1-6,共6页
Objective:To investigate the therapeutic effects and mechanisms of Feng-Liao-Chang-Wei-Kang combined with 5-fluorouracil administration in mice with colitis-associated colon cancer.Methods:To establish a colitis-assoc... Objective:To investigate the therapeutic effects and mechanisms of Feng-Liao-Chang-Wei-Kang combined with 5-fluorouracil administration in mice with colitis-associated colon cancer.Methods:To establish a colitis-associated colon cancer mouse model and observe the behavior and activity of mice after Feng-Liao-Chang-Wei-Kang and 5-fluorouracil administration;HE staining to observe the pathological changes of mouse colonic tissue;Western blot was used to detect the expression of mouse colon tissue in IL-6/STAT3 pathway-related proteins.Results:The survival rate of mice in the co-administered group was significantly increased,and the intestinal wall thickening and interstitial inflammation of mice were significantly reduced.Western blot results showed that the expression levels of P-STAT3 and IL-6 were significantly increased in the colonic tissue of mice after modeling,and the combined administration inhibited the expression of Cyclin D1,CDK4 and Bcl-2 protein in the IL-6/STAT3 pathway and upregulated the expression of Bax(P<0.05).Conclusion:Feng-Liao-Chang-Wei-Kang combined with 5-fluorouracil inhibits IL-6/STAT3 pathway to exert inhibition of colitis-associated colon cancer inhibition of colitis-associated colon cancer. 展开更多
关键词 Colitis-associated colon cancer Feng-Liao-Chang-Wei-Kang 5-fluorouracil IL-6/STAT3
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