目的:探讨干扰三元基序59(TRIM59)表达对慢性粒细胞白血病K562细胞柔红霉素(DNR)化疗敏感性的影响及相关分子机制。方法:采用RT-q PCR法检测慢性粒细胞白血病患者骨髓组织和K562细胞中TRIM59 m RNA表达水平。用脂质体转染法将TRIM59特...目的:探讨干扰三元基序59(TRIM59)表达对慢性粒细胞白血病K562细胞柔红霉素(DNR)化疗敏感性的影响及相关分子机制。方法:采用RT-q PCR法检测慢性粒细胞白血病患者骨髓组织和K562细胞中TRIM59 m RNA表达水平。用脂质体转染法将TRIM59特异性小干扰RNA(si-TRIM59)转染至K562细胞,并用DNR处理细胞。CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot法检测凋亡相关蛋白和Wnt/β-catenin信号通路相关蛋白表达。结果:与初治时骨髓组织相比,化疗耐药时患者骨髓组织中TRIM59 m RNA表达水平升高(P<0.05)。与对照组比较,si-TRIM59组和DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。与si-TRIM59组和DNR组比较,si-TRIM59+DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。结论:干扰TRIM59表达可增强K562细胞对DNR的化疗敏感性,其作用机制可能与调控Wnt/β-catenin信号通路相关。展开更多
Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in th...Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in the integrated management of children aged 6 - 59 months suffering from SAM at the University Hospital of Mother and Child (CHUME) and the Chad-China Friendship Hospital (HATC) of N’Djamena. Methodology: this is a cross-sectional descriptive and interpretative study conducted from January to October 2023 at the CHUME and HATC of N’Djamena. It is based on questionnaires and direct observation of two hundred and thirty-six (236) parents or guardians of malnourished children admitted to these health facilities. All 236 agreed to participate freely in this study. Results: it appears from this study that the obstacles to the integrated management of malnourished children were: poor care-care relationship (26.7%) permanent product breaks (22.9%), the direct costs of services ranged from 30,000 to 60,000 FCFA (41.53%), drugs used for the treatment of patients came from the street (66.10%), malaria and anemia occupy an important place (27.5%), 57.2% respondents had monthly income below 60,000 CFAF. Conclusion: the present study carried out has identified the real bottlenecks to the integrated management of children suffering from SAM in the therapeutic nutritional units of the city of N’Djamena. .展开更多
The nearby bright M-dwarf star L 98–59 has three terrestrial-sized planets.One challenge remaining in characterizing atmospheres around such planets is that it is not known a priori whether they possess any atmospher...The nearby bright M-dwarf star L 98–59 has three terrestrial-sized planets.One challenge remaining in characterizing atmospheres around such planets is that it is not known a priori whether they possess any atmospheres.Here we report on study of the atmospheres of L 98–59 c and L 98–59 d using near-infrared spectral data from the G141 grism of Hubble Space Telescope(HST)/Wide Field Camera 3.We can reject the hypothesis of a clear atmosphere dominated by hydrogen and helium at a confidence level of ~3σ for both planets.Thus they could have a primary hydrogen-dominated atmosphere with an opaque cloud layer,or could have lost their primary hydrogen-dominated atmosphere and re-established a secondary thin atmosphere,or have no atmosphere at all.We cannot distinguish between these scenarios for the two planets using the current HST data.Future observations with the James Webb Space Telescope would be capable of confirming the existence of atmospheres around L 98–59 c and d and determining their compositions.展开更多
文摘目的:探讨干扰三元基序59(TRIM59)表达对慢性粒细胞白血病K562细胞柔红霉素(DNR)化疗敏感性的影响及相关分子机制。方法:采用RT-q PCR法检测慢性粒细胞白血病患者骨髓组织和K562细胞中TRIM59 m RNA表达水平。用脂质体转染法将TRIM59特异性小干扰RNA(si-TRIM59)转染至K562细胞,并用DNR处理细胞。CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot法检测凋亡相关蛋白和Wnt/β-catenin信号通路相关蛋白表达。结果:与初治时骨髓组织相比,化疗耐药时患者骨髓组织中TRIM59 m RNA表达水平升高(P<0.05)。与对照组比较,si-TRIM59组和DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。与si-TRIM59组和DNR组比较,si-TRIM59+DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。结论:干扰TRIM59表达可增强K562细胞对DNR的化疗敏感性,其作用机制可能与调控Wnt/β-catenin信号通路相关。
文摘Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in the integrated management of children aged 6 - 59 months suffering from SAM at the University Hospital of Mother and Child (CHUME) and the Chad-China Friendship Hospital (HATC) of N’Djamena. Methodology: this is a cross-sectional descriptive and interpretative study conducted from January to October 2023 at the CHUME and HATC of N’Djamena. It is based on questionnaires and direct observation of two hundred and thirty-six (236) parents or guardians of malnourished children admitted to these health facilities. All 236 agreed to participate freely in this study. Results: it appears from this study that the obstacles to the integrated management of malnourished children were: poor care-care relationship (26.7%) permanent product breaks (22.9%), the direct costs of services ranged from 30,000 to 60,000 FCFA (41.53%), drugs used for the treatment of patients came from the street (66.10%), malaria and anemia occupy an important place (27.5%), 57.2% respondents had monthly income below 60,000 CFAF. Conclusion: the present study carried out has identified the real bottlenecks to the integrated management of children suffering from SAM in the therapeutic nutritional units of the city of N’Djamena. .
基金the financial support from the National Key R&D Program of China(2020YFC2201400)National Natural Science Foundation of China(NSFC,Grant Nos.12073092,12103097,and 12103098)+5 种基金the science research grants from the China Manned Space Project(No.CMS-CSST-2021-B09,B12)Guangzhou Basic and Applied Basic Research Program(202102080371)the Strategic Priority Program on Space Science,the Chinese Academy of Sciences(Grant No.XDA15020601)China Postdoctoral Science Foundation(No.2020M672936)the Fundamental Research Funds for the Central Universities,Sun Yat-sen University,Hainan Provincial Natural Science Foundation of China under Grant No.122RC546the National Natural Science Foundation of China under grant Nos.12063001。
文摘The nearby bright M-dwarf star L 98–59 has three terrestrial-sized planets.One challenge remaining in characterizing atmospheres around such planets is that it is not known a priori whether they possess any atmospheres.Here we report on study of the atmospheres of L 98–59 c and L 98–59 d using near-infrared spectral data from the G141 grism of Hubble Space Telescope(HST)/Wide Field Camera 3.We can reject the hypothesis of a clear atmosphere dominated by hydrogen and helium at a confidence level of ~3σ for both planets.Thus they could have a primary hydrogen-dominated atmosphere with an opaque cloud layer,or could have lost their primary hydrogen-dominated atmosphere and re-established a secondary thin atmosphere,or have no atmosphere at all.We cannot distinguish between these scenarios for the two planets using the current HST data.Future observations with the James Webb Space Telescope would be capable of confirming the existence of atmospheres around L 98–59 c and d and determining their compositions.
