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Shear strength of extremely altered serpentinites based on degree of saturation(Ankara, Turkey) 被引量:1
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作者 Koray ULAMI? 《Journal of Mountain Science》 SCIE CSCD 2019年第4期944-954,共11页
The term "mélange" has several definitions due to the origin, tectonic and petrographical features. The geotechnical engineering behaviors of mélanges are either dominated by different kinds of int... The term "mélange" has several definitions due to the origin, tectonic and petrographical features. The geotechnical engineering behaviors of mélanges are either dominated by different kinds of intact rock blocks or matrix material. Landslides were encountered within the serpentinite matrix of Ankara Mélange, which is a typical bimrock mass. The residual sections of the extremely altered serpentinites are sandy soils. Such soils undergo rapid change of saturation, leading to shear strength reduction. Undisturbed and disturbed samples were obtained from the outcrops for shear box tests. Geochemical and petrographical composition of the serpentinites were determined by XRF(X Ray Fluorescence), XRD(X-ray diffraction) and thin section inspections, in order to outline the alteration process. Based on field observations, physically decomposed core stones exist beneath 40-120 cm thick residual green and grey-dark grey residual sandy soil. Physical and mechanical properties of the soils were tested with particular emphasis on residual shear strength parameters. Disturbed samples were remolded by standard compaction. The field work was completed during both rainy and summer seasons. Disturbed samples were prepared using phase diagrams in order to attain varying saturation degree. Two distinct sandy soil groups were determined through classification tests. The volumetric compression and/or expansion of the loose and dense samples were also considered based on the angle of dilatancy. A series of consolidated and drained shear box tests were conducted with ascending degree of saturation. The reduction of effective apparent cohesion, internal friction angle and dilatancy angle were determined to obtain a threshold of the degree of saturation. All the sand samples had zero residual internal friction angle and/or apparent cohesion after reaching 70% degree of saturation. 展开更多
关键词 MÉLANGE SERPENTINITE Shear strength Saturation DILATANCY ankara
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Daily Ambrosia Pollen Concentration in the Air of Ankara,Turkey (1990-1999) 被引量:4
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作者 Ayse KAPLAN Nazmiye SAKIYAN Nmǖnevver PINAR 《Acta Botanica Sinica》 CSCD 2003年第12期1408-1412,共5页
The airborne ragweed pollen spectrum was investigated in the air of Ankara, Turkey for aperiod of ten years (1990-1999) using a Burkard seven-day volumetric recording trap. In our study period,long distance transporte... The airborne ragweed pollen spectrum was investigated in the air of Ankara, Turkey for aperiod of ten years (1990-1999) using a Burkard seven-day volumetric recording trap. In our study period,long distance transported Ambrosia pollen has been registered. Daily pollen levels varied from low to highin Burge抯 system. In last three years, the pollen concentration of Ambrosia showed a clear increasingtendency. Our results prove that ragweed pollen may be an important threat for ragweed sensitive patientsin Ankara city in near future. 展开更多
关键词 AMBROSIA RAGWEED long distance transport ankara TurkeyAySe KAPLAN1* Nazmiye SAKIYAN2 N Muevver PINAR2..‘s
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用改良的Ankara痘病毒加强免疫的DNA疫苗的免疫原性
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作者 江丽君 陈锦荣 《生物制品快讯》 2004年第4期18-18,共1页
关键词 ankara痘病毒 DNA疫苗 免疫原性 T细胞 免疫应答
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在未免疫志愿者中抗肝内期恶性疟DNA疫苗及改良痘苗病毒Ankara疫苗的安全性
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作者 王剑霓 《国外医学(预防.诊断.治疗用生物制品分册)》 2004年第3期136-136,共1页
关键词 未免疫志愿者 抗肝内期恶性疟DNA疫苗 痘苗病毒 ankara疫苗 安全性
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The Process of Institutionalization of Electronic Records Management Systems in Universities: Ankara University e-BEYAS Application
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作者 Zeynep Akdogan Fahrettin Ozdemirci 《通讯和计算机(中英文版)》 2016年第1期50-54,共5页
关键词 电子文件管理 档案管理系统 制度化 安卡拉 应用 大学 高校 记录管理系统
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重组结核抗原痘苗病毒Ankara株的构建及其免疫原性研究 被引量:5
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作者 楼觉人 张群 朱琳 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2008年第9期812-817,共6页
目的构建5种不同类型的表达结核杆菌特异抗原的重组痘苗病毒,并研究其特异免疫原性。方法运用同源重组技术将含结核分泌抗原Ag85A和ESAT-6的基因片段插入痘苗病毒表达质粒p18中。重组质粒导入痘苗病毒Ankara(MVA)后构建重组痘苗病毒... 目的构建5种不同类型的表达结核杆菌特异抗原的重组痘苗病毒,并研究其特异免疫原性。方法运用同源重组技术将含结核分泌抗原Ag85A和ESAT-6的基因片段插入痘苗病毒表达质粒p18中。重组质粒导入痘苗病毒Ankara(MVA)后构建重组痘苗病毒,经筛选和Western blot鉴定,得到5个种类的带有结核抗原基因的重组病毒。用构建的5种重组病毒免疫小鼠,MTT法检测免疫后小鼠脾淋巴细胞对特异结核抗原的增殖反应;ELISA检测小鼠脾淋巴细胞培养上清液中IFN-γ的含量;结核菌素纯蛋白衍化物(PPD)皮内试验以检测重组病毒引发的针对结核抗原的特异细胞免疫应答。结果构建的5种重组病毒介导的细胞表达产物经Western blot鉴定确认相对分子质量与结核抗原一致。免疫小鼠两次后,5种重组病毒免疫组脾淋巴细胞体外与Ag85A—ESAT-6融合蛋白共培养后表现出明显的增殖活性(P〈0.01),培养上清液中IFN-γ的浓度均较同组细胞经生理盐水刺激明显增高(P〈0.05);与空痘苗病毒或生理盐水免疫后小鼠相比,5种重组MVA免疫组脾淋巴细胞与Ag85A-ESAT-6融合蛋白共培养后同样表现出明显的增殖活性(P〈0.01),与Ag85A—ESAT-6融合蛋白共培养的细胞上清液中IFN-γ的浓度均升高(P〈0.01)。与空痘苗病毒或生理盐水免疫后小鼠相比,5种重组MVA免疫组小鼠对PPD都表现出显著的迟发型超敏反应应答(P〈0.05)。结论成功构建了5种不同类型的表达结核杆菌抗原的重组痘苗病毒疫苗,其免疫小鼠后可激发针对结核杆菌抗原的特异性细胞免疫。 展开更多
关键词 结核 重组痘苗病毒ankara 疫苗 免疫原性
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禽安卡拉病生物制剂研发及药物防控的研究进展
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作者 罗东还 曹登友 +2 位作者 温贵兰 陈佳琪 龚新勇 《黑龙江畜牧兽医》 CAS 北大核心 2024年第5期31-35,42,共6页
禽安卡拉病又称禽心包积液-肝炎综合征(hydropericardium hepatitis syndrome, HHS),是由Ⅰ群血清4型禽腺病毒(Fowl adenovirus serotype 4,FAdV-4)感染引起的一种严重危害家禽养殖的传染性疾病,主要表现为心包积液、肝脏组织损伤等临... 禽安卡拉病又称禽心包积液-肝炎综合征(hydropericardium hepatitis syndrome, HHS),是由Ⅰ群血清4型禽腺病毒(Fowl adenovirus serotype 4,FAdV-4)感染引起的一种严重危害家禽养殖的传染性疾病,主要表现为心包积液、肝脏组织损伤等临床症状。该病在多个国家和地区相继暴发,给养禽业带来巨大的经济损失。目前国内尚无有效防治禽安卡拉病的商品化疫苗和特效治疗药物,因此需要进一步研究针对该病的生物性防控策略。文章综述了针对FAdV-4感染的疫苗、抗体、药物等生物性防控措施的最新研究进展,以期为禽安卡拉病的生物性防控提供参考依据。 展开更多
关键词 禽安卡拉病 生物性防控 疫苗 抗体 药物
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A Deleted Deletion Site in a New Vector Strain and Exceptional Genomic Stability of Plaque-Purified Modified Vaccinia Ankara(MVA)
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作者 Ingo Jordan Deborah Horn +3 位作者 Kristin Thiele Lars Haag Katharina Fiddeke Volker Sandig 《Virologica Sinica》 SCIE CAS CSCD 2020年第2期212-226,共15页
Vectored vaccines based on highly attenuated modified vaccinia Ankara(MVA) are reported to be immunogenic, tolerant to pre-existing immunity, and able to accommodate and stably maintain very large transgenes. MVA is u... Vectored vaccines based on highly attenuated modified vaccinia Ankara(MVA) are reported to be immunogenic, tolerant to pre-existing immunity, and able to accommodate and stably maintain very large transgenes. MVA is usually produced on primary chicken embryo fibroblasts, but production processes based on continuous cell lines emerge as increasingly robust and cost-effective alternatives. An isolate of a hitherto undescribed genotype was recovered by passage of a nonplaque-purified preparation of MVA in a continuous anatine suspension cell line(CR.pIX) in chemically defined medium.The novel isolate(MVA-CR19) replicated to higher infectious titers in the extracellular volume of suspension cultures and induced fewer syncytia in adherent cultures. We now extend previous studies with the investigation of the point mutations in structural genes of MVA-CR19 and describe an additional point mutation in a regulatory gene. We furthermore map and discuss an extensive rearrangement of the left telomer of MVA-CR19 that appears to have occurred by duplication of the right telomer. This event caused deletions and duplications of genes that may modulate immunologic properties of MVACR19 as a vaccine vector. Our characterizations also highlight the exceptional genetic stability of plaque-purified MVA:although the phenotype of MVA-CR19 appears to be advantageous for replication, we found that all genetic markers that differentiate wildtype and MVA-CR19 are stably maintained in passages of recombinant viruses based on either wildtype or MVA-CR. 展开更多
关键词 Modified vaccinia ankara(MVA) MVA-CR19 CR.pIX Suspension cell line Vaccine production Genome stability
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鸡安卡拉病毒与细菌混合感染的诊治 被引量:1
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作者 杨雪贞 《现代畜牧科技》 2023年第2期92-94,共3页
为明确贵州省天柱县某鸡场鸡群的发病原因,本试验通过流行病学调查、临床解剖病变观察、细菌分离培养、药物敏感试验、病毒检测进行诊断。结果:病鸡精神沉郁,排黄绿色稀粪;剖检可见心包积液、肺水肿、肝肾肿大、输尿管上尿酸盐沉积;脏... 为明确贵州省天柱县某鸡场鸡群的发病原因,本试验通过流行病学调查、临床解剖病变观察、细菌分离培养、药物敏感试验、病毒检测进行诊断。结果:病鸡精神沉郁,排黄绿色稀粪;剖检可见心包积液、肺水肿、肝肾肿大、输尿管上尿酸盐沉积;脏器中分离到革兰氏阴性短小杆菌,对氟苯尼考高度敏感;病毒核酸检测出安卡拉(FAdV-4)病毒为阳性,其他病毒检测为阴性。结论:该病例诊断为鸡安卡拉病毒与细菌混合感染。通过隔离鸡群、环境消毒、紧急接种卵黄抗体并配合药物治疗,病情得到有效控制。 展开更多
关键词 安卡拉病毒 细菌病 混合感染 诊治
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安卡拉病病原分离及抗病毒药物治疗效果研究
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作者 刘建春 《现代畜牧科技》 2023年第3期33-36,共4页
为分离鉴定安卡拉病毒,研究3种抗病毒药物对安卡拉病的治疗效果,试验选用黄芪多糖注射液、干扰素、利巴韦林3种抗病毒药物对240枚SPF鸡胚和240只安卡拉病患病鸡分别用药,记录每组鸡胚的存活数量,再用同样的分组和试验方式选择240只病鸡... 为分离鉴定安卡拉病毒,研究3种抗病毒药物对安卡拉病的治疗效果,试验选用黄芪多糖注射液、干扰素、利巴韦林3种抗病毒药物对240枚SPF鸡胚和240只安卡拉病患病鸡分别用药,记录每组鸡胚的存活数量,再用同样的分组和试验方式选择240只病鸡进行试验,2次试验存活数量最多的组即为治疗安卡拉病效果最优的方法。试验结果表明,最有效的用药方法为黄芪多糖与干扰素合用,其临床治疗中治愈率66.7%。 展开更多
关键词 安卡拉病 接种 药物合用 疗效
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人乳头瘤病毒16型晚期蛋白L1基因重组MVA病毒的构建及鉴定
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作者 刘大维 姜春来 +3 位作者 于湘晖 吴永革 金英花 孔维 《中国生物制品学杂志》 CAS CSCD 2006年第4期352-354,共3页
目的研制预防人乳头瘤病毒(HPV)感染的重组修饰的Ankara痘病毒(MVA)活载体疫苗。方法将HPV16L1基因插入MVA病毒转移载体psc11M2的P7·5启动子的下游,构建转移质粒psc11M2-L1,与MVA共转染鸡胚成纤维细胞(CEF),进行同源重组。通过9轮... 目的研制预防人乳头瘤病毒(HPV)感染的重组修饰的Ankara痘病毒(MVA)活载体疫苗。方法将HPV16L1基因插入MVA病毒转移载体psc11M2的P7·5启动子的下游,构建转移质粒psc11M2-L1,与MVA共转染鸡胚成纤维细胞(CEF),进行同源重组。通过9轮蓝斑筛选,以PCR和Westernblot鉴定重组病毒。结果获得的含目的片段的重组MVA病毒,表达产物与鼠抗人HPV16L1抗体发生阳性反应,目的蛋白相对分子质量约为55000。结论已成功地构建了可正确表达HPV16L1基因的重组MVA病毒株。 展开更多
关键词 人乳头瘤病毒 L1基因 修饰的ankara痘病毒
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治疗性乙型肝炎病毒载体疫苗的构建 被引量:2
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作者 陈宇 杨小松 +1 位作者 李惟 孔维 《中国生物制品学杂志》 CAS CSCD 2006年第5期474-476,共3页
目的研制治疗慢性乙型肝炎的重组修饰的Ankara痘病毒(MVA)载体疫苗。方法将HBV的表面抗原基因克隆到MVA病毒的重组载体pSC11上,重组质粒与空MVA病毒共转染鸡胚成纤维细胞(CEF),进行同源重组。经过10轮筛选,挑选出阳性的重组MVA病毒。经... 目的研制治疗慢性乙型肝炎的重组修饰的Ankara痘病毒(MVA)载体疫苗。方法将HBV的表面抗原基因克隆到MVA病毒的重组载体pSC11上,重组质粒与空MVA病毒共转染鸡胚成纤维细胞(CEF),进行同源重组。经过10轮筛选,挑选出阳性的重组MVA病毒。经PCR鉴定,感染不同细胞系,确定病毒表达量。结果获得目的重组MVA病毒,ELISA检测到表面抗原在不同细胞系中的表达。结论已成功地构建了表达HBV表面抗原的重组MVA病毒。 展开更多
关键词 乙型肝炎 修饰的ankara痘病毒 治疗性疫苗
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恶性疟原虫裂殖子表面蛋白1 DNA与改良痘苗病毒组合疫苗诱导小鼠抗体应答 被引量:3
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作者 李淑梅 李珣 +4 位作者 薛采芳 缪军 雷俊川 刘忠湘 王宪锋 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2005年第2期93-96,共4页
目的探索DNA与改良痘苗病毒(MVA)组合免疫对增强恶性疟原虫裂殖子表面蛋白1(MSP1)抗体应答的作用。方法以人工合成MSP1全基因为基础分别构建DNA免疫质粒VR1020/190和重组MVA,单用VR1020/190或与表达质粒GM-CSF共同对小鼠进行初始免疫后... 目的探索DNA与改良痘苗病毒(MVA)组合免疫对增强恶性疟原虫裂殖子表面蛋白1(MSP1)抗体应答的作用。方法以人工合成MSP1全基因为基础分别构建DNA免疫质粒VR1020/190和重组MVA,单用VR1020/190或与表达质粒GM-CSF共同对小鼠进行初始免疫后,用重组病毒追加强化,采用DNA/MVA组合方案免疫BALB/c小鼠,ELISA测定血清IgG及其亚类水平,经腹腔接种转基因伯氏疟原虫Pb-PfM19进行攻击。结果DNA免疫能有效诱导小鼠产生抗MSP1-190抗体,其终点稀释度为1∶2500,GM-CSF质粒共免疫组抗体的终点稀释度为1∶11150,抗体亚类的测定表明GM-CSF质粒显著促进了IgG1类抗体应答,MVA追加可使单独免疫组和共免疫组抗体分别增加53和10倍;两实验组产生了水平相近的抗19000抗体(1∶32000),其含量占血清中MSP1总IgG的1/4-1/3。经转基因伯氏疟原虫Pb-PfM19攻击后小鼠的存活时间并没有明显延长(P>0.05)。结论采用合成MSP1全基因进行DNA/MVA组合免疫可诱导小鼠产生显著的抗体应答,抗体的详细特性和保护作用正在进一步研究中。 展开更多
关键词 恶性疟原虫裂殖子表面蛋白 抗体应答 痘苗病毒 疫苗诱导 组合 改良 BALB/c小鼠 ELISA测定 DNA免疫 伯氏疟原虫 MSP1 血清IgG MVA CSF 免疫组 人工合成 表达质粒 重组病毒 IgG1 抗体亚类 总IgG 存活时间 MSPI 保护作用
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基于改良型痘苗病毒安卡拉株载体的新型流感疫苗研究进展 被引量:3
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作者 尹元 王伟灵 《中国人兽共患病学报》 CAS CSCD 北大核心 2017年第3期222-227,共6页
流感是由流感病毒引起的一种严重危害公众健康的呼吸道传染病,已引起全球的高度关注,接种疫苗是预防流感最有效的措施。当前,建立快速流感疫苗研发生产平台对于流感的预防与控制至关重要。以病毒为载体的活疫苗为感染性疾病的防控提供... 流感是由流感病毒引起的一种严重危害公众健康的呼吸道传染病,已引起全球的高度关注,接种疫苗是预防流感最有效的措施。当前,建立快速流感疫苗研发生产平台对于流感的预防与控制至关重要。以病毒为载体的活疫苗为感染性疾病的防控提供了新的手段。改良型痘苗病毒安卡拉株(Modified Vaccinia virus Ankara,MVA)是一种复制缺陷型病毒载体。MVA能够同时容纳并表达多个外源抗原、诱导较好的体液和细胞免疫应答并具备良好的安全性,具有成为理想疫苗载体的潜力。本文就MVA载体在流感疫苗研究中的应用做一综述。 展开更多
关键词 流感病毒 改良型痘苗病毒安卡拉株 病毒载体疫苗
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家禽安卡拉病研究进展 被引量:3
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作者 刘畅 图门巴雅尔 +3 位作者 路振香 宁康健 姜锦鹏 李磊 《畜牧与饲料科学》 2019年第8期107-109,共3页
家禽安卡拉病是由禽腺病毒4型(FAV-4)所引起的,以心包积液和肝炎为主要临床表现症状,近年来,该病在我国的发生有扩大的趋势。对家禽安卡拉病的流行病学特点、检测方法及防治方法进行综述,以期为该病的深入研究和防治提供思路。
关键词 家禽 安卡拉病 防治方法 研究进展
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安卡拉地铁底架设备布置及螺栓选型分析 被引量:3
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作者 岳译新 刘厚林 《机车电传动》 北大核心 2015年第4期80-82,共3页
结合不锈钢车辆的特点,综合考虑车辆限界、重量分配、设备安装和维护空间等因素,研究出一个合理的底架设备布置方案,并对螺栓选型进行了分析。经试验和上线运营验证,设备布置满足限界、轴重和轮重偏差及安装维护要求。
关键词 不锈钢车辆 底架 设备布置 重量分配 螺栓选型 安卡拉地铁
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泰安地区一例蛋鸡安卡拉病的初步研究 被引量:2
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作者 宋翔 郭杨丽 +3 位作者 亓秀晔 程福亮 徐海燕 谷巍 《畜禽业》 2018年第11期14-16,共3页
从山东泰安某蛋鸡鸡场疑似安卡拉鸡的脾脏和肝脏组织中分离到一株病毒,经SPF鸡胚连续传代、PCR鉴定和测序初步确定该病毒为鸡安卡拉病毒,命名为TA001株。
关键词 安卡拉病毒 分离 鉴定
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Is It About Time to Rethink American Extended Deterrence?
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作者 Nursin Atesoglu GUNEY 《International Relations and Diplomacy》 2014年第1期55-65,共11页
In today's complex web of relations due to the end of the confrontation between the East and West, nuclear materials, technologies, know-how, and scientists have been diffused to states and non-state actors that act ... In today's complex web of relations due to the end of the confrontation between the East and West, nuclear materials, technologies, know-how, and scientists have been diffused to states and non-state actors that act irrationally most of the time. The precedent example of nuclear North Korea has given rise to new concerns like whether the low levels of military casualties stemming from nuclear or near-nuclear capable countries will damage the credibility of US/North Atlantic Treaty Organization (NATO) extended deterrence. The ambiguity about how the future of the Iranian Geneva interim agreement will be finalized certainly feeds such kinds of concerns among Tehran's neighbors. Till now, both Turkey and South Korea have directly felt and lived through similar kinds of threats. It is true that, during the Cold War years, these two countries have enjoyed the benefit of American assured nuclear security guarantee. However, the current US/NATO extended deterrence due to the mentioned newly arising threats of our times has fallen short of stopping the skirmishes that are occurring at low levels of confrontation. That is why this paper will try to analyze and compare South Korean and Turkish cases so as to comprehend whether there is a necessity to re-think extended deterrence mechanisms that are differently dispatched to meet Ankara's and Seoul's peculiar security needs. 展开更多
关键词 extended deterrence Weapon of mass destruction ankara SEOUL Iran South Korea low levels of skirmishes
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Fatma Aliye Hanlm's Contributions to History Writing
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作者 Aliye F. Mataraci 《International Relations and Diplomacy》 2016年第8期502-507,共6页
Fatma Aliye Harem (1862-1936) was one of the first writers to focus on the women's issues in her novels in the Ottoman Empire. She also published a large number of articles focusing on women in varying journals, in... Fatma Aliye Harem (1862-1936) was one of the first writers to focus on the women's issues in her novels in the Ottoman Empire. She also published a large number of articles focusing on women in varying journals, in addition to her active role in women's associations. Her work did not remain limited with Literature but extended to the other fields such as Education, Sociology, History and Philosophy. This article analyzes her contributions to the Ottoman History via her two historical works: Tarih-i Osmaninin Bir Devre-i Miihimmesi: Kosova Zaferi-Ankara Hezimeti (An Important Era of the Ottoman History: Victory in Kosovo-Defeat in Ankara) published in 1912/1913 and Ahmet Cevdet Papa ve Zaman: (Ahmet Cevdet Pasha and His Time) published in 1914. Her former work analyzes the significance of the Battle of Kosova (1389) and the Battle of Ankara (I 402) for the Ottoman History. Her latter work is a biographical account of his father, Ahmet Cevdet Pasha, who was an Ottoman intellectual, bureaucrat, administrator, and last but not least, a historian. This work focuses on the significance of these two works for Turkish Historiography. 展开更多
关键词 Fatma Aliye Harem history writing Battle of Kosova Battle of ankara Turkish Historiography
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禽安卡拉病研究进展 被引量:6
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作者 常超越 吴同垒 +5 位作者 李蕴玉 张建文 张召兴 张志强 贾青辉 李佩国 《安徽农业科学》 CAS 2017年第33期100-102,共3页
安卡拉病由禽腺病毒C群IV血清型病毒引起,临床上患病鸡以心包积液和肝炎为典型病理变化。近些年,我国不同省份都有病例报道,发病和死亡情况不尽相同,对养禽业造成巨大的经济损失。安卡拉病毒有垂直和水平2种传播方式,可感染多种品系鸡... 安卡拉病由禽腺病毒C群IV血清型病毒引起,临床上患病鸡以心包积液和肝炎为典型病理变化。近些年,我国不同省份都有病例报道,发病和死亡情况不尽相同,对养禽业造成巨大的经济损失。安卡拉病毒有垂直和水平2种传播方式,可感染多种品系鸡群。结合国内外研究,总结了目前安卡拉病毒的生物学特性、流行病学、诊断技术及其防治措施,以期为科学研究和临床应用提供重要的参考。 展开更多
关键词 安卡拉病 腺病毒 研究进展
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