A simple inexpensive method of monitoring hydrolysis of an antibiotic penicillin G (pen G) and subsequent enzyme detection using gold nanoparticles is presented. Gold nanoparticles capped with Cetyl trimethyl ammonium...A simple inexpensive method of monitoring hydrolysis of an antibiotic penicillin G (pen G) and subsequent enzyme detection using gold nanoparticles is presented. Gold nanoparticles capped with Cetyl trimethyl ammonium bromide (CTAB) are synthesized using chemical route. The particles could be used for detection of Penicillin G acylase (PGA) enzyme by incorporating hydrolysis reaction with pen G. This hydrolysis reaction leads to a shift in the surface plasmon band of gold nanoparticles from 527 nm to 545 nm accompanied by a visual colorimetric change in the solution from red to blue. The process is attributed to aggregation of nanoparticles caused due to displacement of CTAB bilayer by byproducts of the hydrolysis reaction. It is proposed that the presence of 0.007 mg/ml of PGA can be detected by a color change of gold nanoparticles solution without requiring any complicated instrument or highly trained operator to conduct the test. The method could also identify the presence of different penicillins by showing different spectral shifts. Thus the work presented here would be useful not only for the detection of the pharmaceutically important drug Pen G, but also represents a general methodology for the detection of enzymes, eg PGA.展开更多
Amino-modified silica hydrogel(N-MSHG)was prepared by a simple sol-gel processing via the cocondensation of commercial silica sol with 3-aminopropyltrieoxysilane.Penicillin G acylase(PGA),a model enzyme,was covalently...Amino-modified silica hydrogel(N-MSHG)was prepared by a simple sol-gel processing via the cocondensation of commercial silica sol with 3-aminopropyltrieoxysilane.Penicillin G acylase(PGA),a model enzyme,was covalently immobilized onto the N-MSHG and then was used for the enzymatic synthesis of amoxicillin.The samples were characterized by Nitrogen sorption analysis,FT-IR and thermal gravimetric analysis(TGA).The results showed that the amino-modified gel was a mesoporous material with an average pore size of 12.64±0.17 nm.The immobilization process was efficient and the immobilized enzyme showed high catalytic efficiency.The yield of the synthesis of amoxicillin in aqueous media was 38%for 2.5 h.This sol-gel preparation is simple and shows prominent potential value in industrial processing.展开更多
文摘A simple inexpensive method of monitoring hydrolysis of an antibiotic penicillin G (pen G) and subsequent enzyme detection using gold nanoparticles is presented. Gold nanoparticles capped with Cetyl trimethyl ammonium bromide (CTAB) are synthesized using chemical route. The particles could be used for detection of Penicillin G acylase (PGA) enzyme by incorporating hydrolysis reaction with pen G. This hydrolysis reaction leads to a shift in the surface plasmon band of gold nanoparticles from 527 nm to 545 nm accompanied by a visual colorimetric change in the solution from red to blue. The process is attributed to aggregation of nanoparticles caused due to displacement of CTAB bilayer by byproducts of the hydrolysis reaction. It is proposed that the presence of 0.007 mg/ml of PGA can be detected by a color change of gold nanoparticles solution without requiring any complicated instrument or highly trained operator to conduct the test. The method could also identify the presence of different penicillins by showing different spectral shifts. Thus the work presented here would be useful not only for the detection of the pharmaceutically important drug Pen G, but also represents a general methodology for the detection of enzymes, eg PGA.
文摘Amino-modified silica hydrogel(N-MSHG)was prepared by a simple sol-gel processing via the cocondensation of commercial silica sol with 3-aminopropyltrieoxysilane.Penicillin G acylase(PGA),a model enzyme,was covalently immobilized onto the N-MSHG and then was used for the enzymatic synthesis of amoxicillin.The samples were characterized by Nitrogen sorption analysis,FT-IR and thermal gravimetric analysis(TGA).The results showed that the amino-modified gel was a mesoporous material with an average pore size of 12.64±0.17 nm.The immobilization process was efficient and the immobilized enzyme showed high catalytic efficiency.The yield of the synthesis of amoxicillin in aqueous media was 38%for 2.5 h.This sol-gel preparation is simple and shows prominent potential value in industrial processing.
