This review summarizes the main chemical composition of Aloe vera flowers and introduces their antioxidant,anti-aging,skin whitening,skin moisturizing,anti-skin irritation,and anti-skin allergy effects.Suggestions for...This review summarizes the main chemical composition of Aloe vera flowers and introduces their antioxidant,anti-aging,skin whitening,skin moisturizing,anti-skin irritation,and anti-skin allergy effects.Suggestions for the application of Aloe v.flowers in cosmetics are provided.展开更多
Aloe vera plant is known worldwide for its medicinal properties and application in gel-based products such as shampoo,soap,and sunscreen.However,the demand for these gel-based products has led to a surplus production ...Aloe vera plant is known worldwide for its medicinal properties and application in gel-based products such as shampoo,soap,and sunscreen.However,the demand for these gel-based products has led to a surplus production of Aloe vera processing waste.An Aloe vera gel processing facility could generate up to 4000 kg of Aloe vera waste per month.Currently the Aloe vera waste is being disposed to the landfill or used as fertilizer.A sustainable management system for the Aloe vera processing waste should be considered,due to the negative societal and environmental impacts of the currents waste disposal methods.Therefore,this review focuses on various approaches that can be used to valorize Aloe vera waste into value-added products,such as animal and aquaculture feeds,biosorbents,biofuel and natural polymers.Researchers have reported Aloe vera waste for environmental applications biosorbents used for wastewater treatment of various pollutants.Several studies have also reported on the valorization of Aloe vera waste for production of biofuels such as bioethanol,mixed alcohol fuels,biogas and syngas.Aloe vera waste could also be valorized through isolation and synthesis of natural polymers for application in wound dressing,tissue engineering and drug delivery systems.Aloe vera waste valorization was also reviewed through extraction of value-added bioactive compounds such as aloe-emodin,aloin and aloeresin.These value-added bioactive compounds have various applications in the cosmetics(non-steroidal anti-inflammatory,tyrosinase inhibitors)and pharmaceutical(anticancer agent and COVID 19 inhibitors)industry.展开更多
The search for functional foods with health benefits has increased,and yogurt represents one of these products due to their properties,such as,source of probiotics that favour the gastrointestinal system,contains vita...The search for functional foods with health benefits has increased,and yogurt represents one of these products due to their properties,such as,source of probiotics that favour the gastrointestinal system,contains vitamins,minerals and improves the action of enzymes.Present work investigated bromatological,microbiological,sensorial properties and shelf-life of different yogurt goat milk formulations with Aloe vera and natural sweeteners.The goat yogurt with 99.40%A.vera pulp and 0.60%S.rebaudiana presented the best nutritional composition,being suitable for human consumption representing an innovative product with a great functional potential.The synergy between the ingredients used in the yogurt processing resulted in an innovative product to the market.Goat milk has a lower cost per liter in comparison with cow milk,Aloe vera is accessible due to its fast-vegetative propagation,Stevia Rebaudiana has a great popularity for being a natural sweetener with no calories,and all these products are produced in México.展开更多
Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against diff...Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.展开更多
Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinob...Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinobacteria was isolated from leaves of medicinal plant Aloe vera collected in Marrakesh, Morocco using Bennett agar as selective medium. NAF-1 was tested for its antimicrobial activity against five pathogenic bacteria such as Staphylococcus aureus PIC 53156, Micrococcus luteus ATCC381, Bacillus subtilis ATCC 14579, Pseudomonas aeruginosa DSM 50090 and Escherichia coli ATCC 8739 and four human clinic fungi belonging to the Candida, Aspergillus and Microsporum genera. Several antioxidant activities were studied such as DPPH free radical scavenging, β-carotene and linoleic acid and reducing power assays. The total of phenol and flavonoid was also calculated. Using Artemia salina shrimp assay, the cytotoxicity of NAF-1 crude extract was determined. Results: The results revealed that the actinobacteria showed a high activity(≥20 mm) against only Gram positive bacteria but it had a moderate activity(between 13 and 15 mm) against Human clinic fungi. The isolate also exhibited a LD50 of 14.20 μg/mL in the cytotoxicity assay. The result showed that the crude extract presented an interesting free radical-scavenging activity with IC50 value of(5.58 ± 0.26) μg/mL and a high value of phenolic and flavonoid compounds with(15.41 ± 0.18) μg GAE/mg extract and(11.41± 0.06) μg QE/mg extract respectively. Moreover, the taxonomic position of our endophyte actinobacteria using the morphological and physiological criteria and using 16 S r RNA gene sequence(polyphasic approach) showed that the NAF-1 isolate was similar to Streptomyces hydrogenans which was never described as an endophyte actinobacteria. Conclusions: This isolated strain appears promising resources of bioactive agents and can be exploited to produce therapeutic agents active against pathogenic disease.展开更多
AIM:To evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin(IMN)-induced gastropathy.METHODS:Male Sprague-Dawley rats were randomly divided into three groups.Group 1(control,n=6)was...AIM:To evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin(IMN)-induced gastropathy.METHODS:Male Sprague-Dawley rats were randomly divided into three groups.Group 1(control,n=6)was given distilled water(DW)orally.Group 2(IMN,n=6)was given oral IMN(150 mg/kg)dissolved in5%sodium bicarbonate(Na HCO3-)at time 0 and 4 h.Group 3(Aloe vera-treated,n=6)was given oral Aloevera(150 mg/kg)dissolved in DW and IMN at time 0and 4 h.Eight hours later,the stomach was removed to determine gastric malondialdehyde(MDA),the number of interleukin(IL)-18 positive stained cells(%)by immunohistochemistry,and for histopathological examination.Then,the serum was collected to determine tumor necrosis factor(TNF)-αand cytokineinduced neutrophil chemoattractant(CINC)-1 by sandwich enzyme linked immunosorbent assay method.RESULTS:In the IMN group,serum TNF-α,CINC-1and gastric MDA were significantly increased when compared to the control group(27.78±1.52 pg/m L vs 85.07±49.11 pg/m L,P=0.009;104.55±45.80pg/m L vs 1054.70±20.38 pg/m L,and 1.74±0.21nmol/mg vs 9.36±1.07 nmol/mg protein,P=0.000,respectively).The mean level of TNF-α,CINC-1 and gastric MDA in the Aloe vera-treated group were improved as compared with the IMN group(85.07±49.11 pg/m L vs 35.19±1.61 pg/m L,P=0.021;1054.70±20.38 pg/m L vs 813.56±239.04 pg/m L,P=0.025;and 9.36±1.07 nmol/mg vs 2.67±0.64 nmol/mg protein,P=0.000,respectively).The number of IL-18 positive stained cells(%)in the gastric epithelial cells of the IMN group was significantly higher than the control group(5.01%±3.73%vs 30.67%±2.03%,P=0.000,respectively).In contrast,Aloe vera treatment decreased the number of IL-18 positive stained cells(%)significantly when compared with the IMN group(30.67%±2.03%vs 13.21%±1.10%,P=0.000,respectively).Most rats in the IMN group developed moderate to severe gastric inflammation and erosions.The gastric erosions and neutrophil infiltration scores were significantly reduced in the Aloe veratreated group.CONCLUSION:Aloe vera attenuated IMN-induced gastropathy in rats by the reduction of oxidative stress,inflammation,and improvement of gastrichistopathology.展开更多
The unique structural and physical properties of boron carbide, which make it suitable for a wide range of applications,demands the development of low-cost and green synthesis method. In the present work, the commonly...The unique structural and physical properties of boron carbide, which make it suitable for a wide range of applications,demands the development of low-cost and green synthesis method. In the present work, the commonly available leaves of aloe vera are hydrothermally treated to form the carbon precursor for the synthesis of boron carbide. The morphological characterization reveals the porous nature of the precursor turning into a tubular structure upon boron carbide formation.The structural characterization by x-ray diffraction and other spectroscopic techniques such as Fourier transform infrared,Raman, photoluminescence and uv-visible near-infrared spectroscopy confirm the formation of boron carbide. The thermogravimetric analysis of the sample is found to exhibit good thermal stability above 500 °C. When the sample is annealed to 600 °C, boron carbide with phase purity is obtained, which is confirmed through XRD and FTIR analyses. The optical emission properties of the sample are studied through CIE plot and power spectrum. Compared with other natural precursors for boron carbide, the aloe vera is found to give a good yield above 50%.展开更多
The plant Aloe vera is used in Ayurvedic, Homoeopathic and Allopathic streams of medicine, and not only tribal community but also most of the people for food and medicine. The plant leaves contains numerous vitamins, ...The plant Aloe vera is used in Ayurvedic, Homoeopathic and Allopathic streams of medicine, and not only tribal community but also most of the people for food and medicine. The plant leaves contains numerous vitamins, minerals, enzymes, amino acids, natural sugars and other bioactive compounds with emollient, purgative, anti-microbial, anti inflammatory, antioxidant, aphrodisiac, anti-helmenthic, antifungal, antiseptic and cosmetic values for health care. This plant has potential to cure sunburns, burns and minor cuts, and even skin cancer. The external use in cosmetic primarily acts as skin healer and prevents injury of epithelial tissues, cures acne and gives a youthful glow to skin, also acts as extremely powerful laxative.展开更多
AIM: Aloe vera, plant extracts of Aloe barbadensis miller,is widely used in phytomedicine. The first case of acute hepatitis due to this compound was described.METHODS: Description of a clinical case.RESULTS: Hepatiti...AIM: Aloe vera, plant extracts of Aloe barbadensis miller,is widely used in phytomedicine. The first case of acute hepatitis due to this compound was described.METHODS: Description of a clinical case.RESULTS: Hepatitis in a 57-year old female could be linked to the ingestion of Aloe barbadensis miller compounds. The patient's hepatitis resolved completely after discontinuing this medication.CONCLUSION: The case emphasizes the importance of considering phytopharmaceutical over-the-counter drugs as causative agents of hepatitis.展开更多
AIM:To investigate the anti-oxidative and anti-fibrotic effects of aloe vera in patients with liver fibrosis.METHODS:Aloe vera high molecular weight fractions(AHM) were processed by patented hyper-dry system in combin...AIM:To investigate the anti-oxidative and anti-fibrotic effects of aloe vera in patients with liver fibrosis.METHODS:Aloe vera high molecular weight fractions(AHM) were processed by patented hyper-dry system in combination of freeze-dry technique with microwave and far infrared-ray radiation.Fifteen healthy volunteers as the control group and 40 patients were included.The patients were randomly subdivided into two equal groups:the conventional group was treated with placebo(starch),and AHM group was treated with 0.15 gm/d AHM,both for 12 consecutive weeks.The patients were investigated before and after treatment.Serum activity of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),hyaluronic acid(HA),transforming growth factor-β(TGF-β) and matrixmetalloproteinase-2(MMP-2) were determined.The reduced glutathione(GSH) and malondialdehyde(MDA) levels in liver were assayed and the expression of hepatic α-smooth muscle actin(α-SMA) was identified by immunohistochemistry.RESULTS:At the start of the study,the hematoxylin and eosin staining revealed fibro-proliferated bile ductules,thick fibrous septa and dense inflammatory cellular infiltration in the patients before treatment.The use of AHM for 12 wk significantly ameliorated the fibrosis,inhibited the inflammation,and resulted in minimal infiltration and minimal fibrosis compared to the conventional group.The enzyme activities of the liver(ALT,AST and ALP) were attenuated after treatment in both groups,and the decrease in the AHM group was more significant as compared with the conventional group.Similar to the AST,the MDA levels were significantly higher before treatment,and were attenuated after treatment in both groups.In contrast,the hepatic glutathione content in the patients were decreased significantly in the AHM group compared to the controls.The serum levels of the fibrosis markers(HA,TGF-β and MMP-2) were also reduced significantly after treatment.The expression of α-SMA was modified in patients before and after treatment as compared with the normal controls.In the conventional group,there was only thin and incomplete parenchymal α-SMA positive septum joining the thickened centrilobular veins,while in the AHM group,few α-SMA positive cells were present in sinusoid and lobule after treatment.CONCLUSION:Oral supplementation with AHM could be helpful in alleviating the fibrosis and inflammation of hepatic fibrosis patients.展开更多
Aloe vera, a short-stemmed shrub is described as a “wonder plant”, due to its vast uses in various medical products. Since many decades, extensive research has revealed that the pharmacological active ingredients ar...Aloe vera, a short-stemmed shrub is described as a “wonder plant”, due to its vast uses in various medical products. Since many decades, extensive research has revealed that the pharmacological active ingredients are distributed in both the gel and rind of the Aloe vera leaves. A. vera is very popular in cosmetic and pharmaceutical industries and it is needed in large quantities with higher fractions of important constituents. To satisfy the market demand, intervention of microbial community seems to be a promising approach, which helps to increase the growth and metabolites along with plant fitness. Piriformospora indica is a root colonizing endophytic fungus, having unique plant growth-promoting properties. It helps the plant to acquire more nutrients from soil even under extreme physical and nutrient stress conditions. It interacts with a wide range of hosts. Interaction of P. indica with A. vera resulted in overall increase in plant biomass and greater shoot and root length, as well as number of shoots and roots as compared to control under both in vitro and in vivo environment conditions. Apart from that, the photosynthetic pigments (Chl a, Chl b and total Chl) and aloin content were observed significantly higher in A. vera plantlets colonized with symbiotic endophyte. The antioxidant activities were also tested and found significantly higher as compared to control plants. This imparts the potential of P. indica, to resist the plants against phyto-pathogenic microbes. P. indica has been proved as a potential candidate to enhance the biomass production along with various value additions in the form of active ingredients in A. vera.展开更多
We have developed a novel methodology for the determination of Aloins A and B in Aloe vera plant extract and commercial Aloe vera product formulations using HPLC method. The result shows good sensitivity and accuracy ...We have developed a novel methodology for the determination of Aloins A and B in Aloe vera plant extract and commercial Aloe vera product formulations using HPLC method. The result shows good sensitivity and accuracy which can be utilized for the scientific validation of bioactive compounds present in the commercial formulations.展开更多
BACKGROUND Aloe vera exerts several biological activities,such as,anti-inflammatory,antioxidant,and antimicrobial effects.It was recently shown to reduce insulin resistance and triglyceride level.We hypothesized that ...BACKGROUND Aloe vera exerts several biological activities,such as,anti-inflammatory,antioxidant,and antimicrobial effects.It was recently shown to reduce insulin resistance and triglyceride level.We hypothesized that aloe vera would have beneficial effects in alleviating non-alcoholic steatohepatitis(NASH)in rats.AIM To examine the therapeutic effects of aloe vera in NASH rats.METHODS All rats were randomly divided into 3 groups(n=6 in each group).Rats in the control group were fed ad libitum with a standard diet for 8 wk.Rats in the NASH group were fed ad libitum with a high-fat high-fructose diet(HFHFD)for 8 wk.Rats in the aloe vera group were fed ad libitum with a HFHFD and aloe vera in dimethylsulfoxide(50 mg/kg)by gavage daily for 8 wk.Liver samples were collected at the end of the treatment period.RESULTS Hepatic malondialdehyde(MDA)levels increased significantly in the NASH group as compared with the control group(377±77 nmol/mg vs 129±51 nmol/mg protein,respectively,P<0.001).Glutathione(GSH)levels were significantly lower in the NASH group than the control group(9±2 nmol/mg vs 24±8 nmol/mg protein,respectively,P=0.001).The expression of interleukin-18 (IL-18), nuclear factor-kappa β, and caspase-3 increased, while peroxisomeproliferator-activated receptor gamma decreased in the NASH group comparedwith the controls. Following aloe vera administration, MDA levels decreased (199± 35 nmol/mg protein) and GSH increased (18 ± 4 nmol/mg protein) markedly.Steatosis, hepatocyte ballooning, lobular inflammation and increased hepatocyteapoptosis were observed in the NASH group. Aloe vera treatment attenuatedthese changes in liver histology.CONCLUSIONAloe vera attenuated oxidative stress, hepatic inflammation and hepatocyteapoptosis, thus improving liver pathology in rats with NASH.展开更多
Objective:Aloe Vera gel(AVG) has a prominent role as a contemporary folk remedy.The Non-healed chronic ulcers of 16 non-responders to conventional treatment were treated with AVG for which the clinical efficacy was st...Objective:Aloe Vera gel(AVG) has a prominent role as a contemporary folk remedy.The Non-healed chronic ulcers of 16 non-responders to conventional treatment were treated with AVG for which the clinical efficacy was studied.Methods:This study included 16 patients having chronic ulcer(9 males and 7 females) with mean age of 41.8±14.6 who following failure treatment to conventional therapy,were dispensed AVG to apply. AVG is consisted from gel part of Aloe Vera plant plus one preservative material,in this study ECG jelly. The patients were assessed as responder whenever intensity of inflammation,the number and /or the size of ulcers have been reduced.Results:The period of time till healing(complete or reduction in size of ulcer), were from 5 to 160 days with mean of 31.2±36.4 days.The mean time of decreasing for secreting,swelling and redness was from 5-11 days.Spearman coefficient of correlation,showed no significant correlation between first size and depth of ulcer and timing till 50%reduction in size of ulcer.Conclusion:All patients had been treated with conventional treatment all to no avail.There were some full thickness ulcer which at the end of treatment we observed an improvement and normal skin for those ulcers.There was a minor complication like itching in one patient but no leading to stop treatment.These findings suggest that AVG is an excellent and safer choice than current conventional therapeutic management of chronic ulcers.展开更多
Background: Aloe vera has been used by many civilizations throughout history due to the numerous properties attributed. Gastro oesophageal reflux disease is a common disorder with consequences for the patient’s healt...Background: Aloe vera has been used by many civilizations throughout history due to the numerous properties attributed. Gastro oesophageal reflux disease is a common disorder with consequences for the patient’s health related quality of life. Aims: The purpose of this study was to assess the benefits of the Aloe vera in 80 patients with gastro oesophageal reflux. Methods: This is probably the first randomised and double-blind trail ever performed on this subject. The study included eight weeks of treatment with milk enriched with Aloe vera or placebo. Data of diet (frequency questionnaire), symptoms (gastrointestinal symptom rating scale) and quality of life (quality of life in reflux and disease) were obtained. Results: So far, there has been hardly any proof that Aloe vera decreases symptoms of reflux disease;on the contrary, a lower percentage of remission of pain is observed among the Aloe vera group. As long as its effectiveness has not been proven properly, the use of Aloe vera in reflux disease cannot be recommend. Conclusion: Aloe vera does not have a beneficial effect on reflux disease under the study conditions.展开更多
文摘This review summarizes the main chemical composition of Aloe vera flowers and introduces their antioxidant,anti-aging,skin whitening,skin moisturizing,anti-skin irritation,and anti-skin allergy effects.Suggestions for the application of Aloe v.flowers in cosmetics are provided.
文摘Aloe vera plant is known worldwide for its medicinal properties and application in gel-based products such as shampoo,soap,and sunscreen.However,the demand for these gel-based products has led to a surplus production of Aloe vera processing waste.An Aloe vera gel processing facility could generate up to 4000 kg of Aloe vera waste per month.Currently the Aloe vera waste is being disposed to the landfill or used as fertilizer.A sustainable management system for the Aloe vera processing waste should be considered,due to the negative societal and environmental impacts of the currents waste disposal methods.Therefore,this review focuses on various approaches that can be used to valorize Aloe vera waste into value-added products,such as animal and aquaculture feeds,biosorbents,biofuel and natural polymers.Researchers have reported Aloe vera waste for environmental applications biosorbents used for wastewater treatment of various pollutants.Several studies have also reported on the valorization of Aloe vera waste for production of biofuels such as bioethanol,mixed alcohol fuels,biogas and syngas.Aloe vera waste could also be valorized through isolation and synthesis of natural polymers for application in wound dressing,tissue engineering and drug delivery systems.Aloe vera waste valorization was also reviewed through extraction of value-added bioactive compounds such as aloe-emodin,aloin and aloeresin.These value-added bioactive compounds have various applications in the cosmetics(non-steroidal anti-inflammatory,tyrosinase inhibitors)and pharmaceutical(anticancer agent and COVID 19 inhibitors)industry.
文摘The search for functional foods with health benefits has increased,and yogurt represents one of these products due to their properties,such as,source of probiotics that favour the gastrointestinal system,contains vitamins,minerals and improves the action of enzymes.Present work investigated bromatological,microbiological,sensorial properties and shelf-life of different yogurt goat milk formulations with Aloe vera and natural sweeteners.The goat yogurt with 99.40%A.vera pulp and 0.60%S.rebaudiana presented the best nutritional composition,being suitable for human consumption representing an innovative product with a great functional potential.The synergy between the ingredients used in the yogurt processing resulted in an innovative product to the market.Goat milk has a lower cost per liter in comparison with cow milk,Aloe vera is accessible due to its fast-vegetative propagation,Stevia Rebaudiana has a great popularity for being a natural sweetener with no calories,and all these products are produced in México.
文摘Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.
文摘Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinobacteria was isolated from leaves of medicinal plant Aloe vera collected in Marrakesh, Morocco using Bennett agar as selective medium. NAF-1 was tested for its antimicrobial activity against five pathogenic bacteria such as Staphylococcus aureus PIC 53156, Micrococcus luteus ATCC381, Bacillus subtilis ATCC 14579, Pseudomonas aeruginosa DSM 50090 and Escherichia coli ATCC 8739 and four human clinic fungi belonging to the Candida, Aspergillus and Microsporum genera. Several antioxidant activities were studied such as DPPH free radical scavenging, β-carotene and linoleic acid and reducing power assays. The total of phenol and flavonoid was also calculated. Using Artemia salina shrimp assay, the cytotoxicity of NAF-1 crude extract was determined. Results: The results revealed that the actinobacteria showed a high activity(≥20 mm) against only Gram positive bacteria but it had a moderate activity(between 13 and 15 mm) against Human clinic fungi. The isolate also exhibited a LD50 of 14.20 μg/mL in the cytotoxicity assay. The result showed that the crude extract presented an interesting free radical-scavenging activity with IC50 value of(5.58 ± 0.26) μg/mL and a high value of phenolic and flavonoid compounds with(15.41 ± 0.18) μg GAE/mg extract and(11.41± 0.06) μg QE/mg extract respectively. Moreover, the taxonomic position of our endophyte actinobacteria using the morphological and physiological criteria and using 16 S r RNA gene sequence(polyphasic approach) showed that the NAF-1 isolate was similar to Streptomyces hydrogenans which was never described as an endophyte actinobacteria. Conclusions: This isolated strain appears promising resources of bioactive agents and can be exploited to produce therapeutic agents active against pathogenic disease.
基金Supported by 90th Anniversary Fund of Chulalongkorn University(Ratchada phiseksomphot Endowment Fund)The Grant of Ratchadaphiseksomphot,Faculty of Medicine,Chulalongkorn University,Bangkok,Thailand
文摘AIM:To evaluate the protective effects of Aloe vera on gastric injury in rats with indomethacin(IMN)-induced gastropathy.METHODS:Male Sprague-Dawley rats were randomly divided into three groups.Group 1(control,n=6)was given distilled water(DW)orally.Group 2(IMN,n=6)was given oral IMN(150 mg/kg)dissolved in5%sodium bicarbonate(Na HCO3-)at time 0 and 4 h.Group 3(Aloe vera-treated,n=6)was given oral Aloevera(150 mg/kg)dissolved in DW and IMN at time 0and 4 h.Eight hours later,the stomach was removed to determine gastric malondialdehyde(MDA),the number of interleukin(IL)-18 positive stained cells(%)by immunohistochemistry,and for histopathological examination.Then,the serum was collected to determine tumor necrosis factor(TNF)-αand cytokineinduced neutrophil chemoattractant(CINC)-1 by sandwich enzyme linked immunosorbent assay method.RESULTS:In the IMN group,serum TNF-α,CINC-1and gastric MDA were significantly increased when compared to the control group(27.78±1.52 pg/m L vs 85.07±49.11 pg/m L,P=0.009;104.55±45.80pg/m L vs 1054.70±20.38 pg/m L,and 1.74±0.21nmol/mg vs 9.36±1.07 nmol/mg protein,P=0.000,respectively).The mean level of TNF-α,CINC-1 and gastric MDA in the Aloe vera-treated group were improved as compared with the IMN group(85.07±49.11 pg/m L vs 35.19±1.61 pg/m L,P=0.021;1054.70±20.38 pg/m L vs 813.56±239.04 pg/m L,P=0.025;and 9.36±1.07 nmol/mg vs 2.67±0.64 nmol/mg protein,P=0.000,respectively).The number of IL-18 positive stained cells(%)in the gastric epithelial cells of the IMN group was significantly higher than the control group(5.01%±3.73%vs 30.67%±2.03%,P=0.000,respectively).In contrast,Aloe vera treatment decreased the number of IL-18 positive stained cells(%)significantly when compared with the IMN group(30.67%±2.03%vs 13.21%±1.10%,P=0.000,respectively).Most rats in the IMN group developed moderate to severe gastric inflammation and erosions.The gastric erosions and neutrophil infiltration scores were significantly reduced in the Aloe veratreated group.CONCLUSION:Aloe vera attenuated IMN-induced gastropathy in rats by the reduction of oxidative stress,inflammation,and improvement of gastrichistopathology.
基金support from UGC-SAP (DRS, Phase Ⅲ) with Sanction order No. F.510/3/DRS-Ⅲ/2015(SAPI)UGC-MRP with F. No. 43-539/2014 (SR)FD Diary No.3668
文摘The unique structural and physical properties of boron carbide, which make it suitable for a wide range of applications,demands the development of low-cost and green synthesis method. In the present work, the commonly available leaves of aloe vera are hydrothermally treated to form the carbon precursor for the synthesis of boron carbide. The morphological characterization reveals the porous nature of the precursor turning into a tubular structure upon boron carbide formation.The structural characterization by x-ray diffraction and other spectroscopic techniques such as Fourier transform infrared,Raman, photoluminescence and uv-visible near-infrared spectroscopy confirm the formation of boron carbide. The thermogravimetric analysis of the sample is found to exhibit good thermal stability above 500 °C. When the sample is annealed to 600 °C, boron carbide with phase purity is obtained, which is confirmed through XRD and FTIR analyses. The optical emission properties of the sample are studied through CIE plot and power spectrum. Compared with other natural precursors for boron carbide, the aloe vera is found to give a good yield above 50%.
文摘The plant Aloe vera is used in Ayurvedic, Homoeopathic and Allopathic streams of medicine, and not only tribal community but also most of the people for food and medicine. The plant leaves contains numerous vitamins, minerals, enzymes, amino acids, natural sugars and other bioactive compounds with emollient, purgative, anti-microbial, anti inflammatory, antioxidant, aphrodisiac, anti-helmenthic, antifungal, antiseptic and cosmetic values for health care. This plant has potential to cure sunburns, burns and minor cuts, and even skin cancer. The external use in cosmetic primarily acts as skin healer and prevents injury of epithelial tissues, cures acne and gives a youthful glow to skin, also acts as extremely powerful laxative.
文摘AIM: Aloe vera, plant extracts of Aloe barbadensis miller,is widely used in phytomedicine. The first case of acute hepatitis due to this compound was described.METHODS: Description of a clinical case.RESULTS: Hepatitis in a 57-year old female could be linked to the ingestion of Aloe barbadensis miller compounds. The patient's hepatitis resolved completely after discontinuing this medication.CONCLUSION: The case emphasizes the importance of considering phytopharmaceutical over-the-counter drugs as causative agents of hepatitis.
文摘AIM:To investigate the anti-oxidative and anti-fibrotic effects of aloe vera in patients with liver fibrosis.METHODS:Aloe vera high molecular weight fractions(AHM) were processed by patented hyper-dry system in combination of freeze-dry technique with microwave and far infrared-ray radiation.Fifteen healthy volunteers as the control group and 40 patients were included.The patients were randomly subdivided into two equal groups:the conventional group was treated with placebo(starch),and AHM group was treated with 0.15 gm/d AHM,both for 12 consecutive weeks.The patients were investigated before and after treatment.Serum activity of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),hyaluronic acid(HA),transforming growth factor-β(TGF-β) and matrixmetalloproteinase-2(MMP-2) were determined.The reduced glutathione(GSH) and malondialdehyde(MDA) levels in liver were assayed and the expression of hepatic α-smooth muscle actin(α-SMA) was identified by immunohistochemistry.RESULTS:At the start of the study,the hematoxylin and eosin staining revealed fibro-proliferated bile ductules,thick fibrous septa and dense inflammatory cellular infiltration in the patients before treatment.The use of AHM for 12 wk significantly ameliorated the fibrosis,inhibited the inflammation,and resulted in minimal infiltration and minimal fibrosis compared to the conventional group.The enzyme activities of the liver(ALT,AST and ALP) were attenuated after treatment in both groups,and the decrease in the AHM group was more significant as compared with the conventional group.Similar to the AST,the MDA levels were significantly higher before treatment,and were attenuated after treatment in both groups.In contrast,the hepatic glutathione content in the patients were decreased significantly in the AHM group compared to the controls.The serum levels of the fibrosis markers(HA,TGF-β and MMP-2) were also reduced significantly after treatment.The expression of α-SMA was modified in patients before and after treatment as compared with the normal controls.In the conventional group,there was only thin and incomplete parenchymal α-SMA positive septum joining the thickened centrilobular veins,while in the AHM group,few α-SMA positive cells were present in sinusoid and lobule after treatment.CONCLUSION:Oral supplementation with AHM could be helpful in alleviating the fibrosis and inflammation of hepatic fibrosis patients.
文摘Aloe vera, a short-stemmed shrub is described as a “wonder plant”, due to its vast uses in various medical products. Since many decades, extensive research has revealed that the pharmacological active ingredients are distributed in both the gel and rind of the Aloe vera leaves. A. vera is very popular in cosmetic and pharmaceutical industries and it is needed in large quantities with higher fractions of important constituents. To satisfy the market demand, intervention of microbial community seems to be a promising approach, which helps to increase the growth and metabolites along with plant fitness. Piriformospora indica is a root colonizing endophytic fungus, having unique plant growth-promoting properties. It helps the plant to acquire more nutrients from soil even under extreme physical and nutrient stress conditions. It interacts with a wide range of hosts. Interaction of P. indica with A. vera resulted in overall increase in plant biomass and greater shoot and root length, as well as number of shoots and roots as compared to control under both in vitro and in vivo environment conditions. Apart from that, the photosynthetic pigments (Chl a, Chl b and total Chl) and aloin content were observed significantly higher in A. vera plantlets colonized with symbiotic endophyte. The antioxidant activities were also tested and found significantly higher as compared to control plants. This imparts the potential of P. indica, to resist the plants against phyto-pathogenic microbes. P. indica has been proved as a potential candidate to enhance the biomass production along with various value additions in the form of active ingredients in A. vera.
文摘We have developed a novel methodology for the determination of Aloins A and B in Aloe vera plant extract and commercial Aloe vera product formulations using HPLC method. The result shows good sensitivity and accuracy which can be utilized for the scientific validation of bioactive compounds present in the commercial formulations.
基金Supported by the Grant of Ratchadaphiseksomphot,Faculty of Medicine,Chulalongkorn University,Bangkok,Thailand,No.RA59/035.
文摘BACKGROUND Aloe vera exerts several biological activities,such as,anti-inflammatory,antioxidant,and antimicrobial effects.It was recently shown to reduce insulin resistance and triglyceride level.We hypothesized that aloe vera would have beneficial effects in alleviating non-alcoholic steatohepatitis(NASH)in rats.AIM To examine the therapeutic effects of aloe vera in NASH rats.METHODS All rats were randomly divided into 3 groups(n=6 in each group).Rats in the control group were fed ad libitum with a standard diet for 8 wk.Rats in the NASH group were fed ad libitum with a high-fat high-fructose diet(HFHFD)for 8 wk.Rats in the aloe vera group were fed ad libitum with a HFHFD and aloe vera in dimethylsulfoxide(50 mg/kg)by gavage daily for 8 wk.Liver samples were collected at the end of the treatment period.RESULTS Hepatic malondialdehyde(MDA)levels increased significantly in the NASH group as compared with the control group(377±77 nmol/mg vs 129±51 nmol/mg protein,respectively,P<0.001).Glutathione(GSH)levels were significantly lower in the NASH group than the control group(9±2 nmol/mg vs 24±8 nmol/mg protein,respectively,P=0.001).The expression of interleukin-18 (IL-18), nuclear factor-kappa β, and caspase-3 increased, while peroxisomeproliferator-activated receptor gamma decreased in the NASH group comparedwith the controls. Following aloe vera administration, MDA levels decreased (199± 35 nmol/mg protein) and GSH increased (18 ± 4 nmol/mg protein) markedly.Steatosis, hepatocyte ballooning, lobular inflammation and increased hepatocyteapoptosis were observed in the NASH group. Aloe vera treatment attenuatedthese changes in liver histology.CONCLUSIONAloe vera attenuated oxidative stress, hepatic inflammation and hepatocyteapoptosis, thus improving liver pathology in rats with NASH.
文摘Objective:Aloe Vera gel(AVG) has a prominent role as a contemporary folk remedy.The Non-healed chronic ulcers of 16 non-responders to conventional treatment were treated with AVG for which the clinical efficacy was studied.Methods:This study included 16 patients having chronic ulcer(9 males and 7 females) with mean age of 41.8±14.6 who following failure treatment to conventional therapy,were dispensed AVG to apply. AVG is consisted from gel part of Aloe Vera plant plus one preservative material,in this study ECG jelly. The patients were assessed as responder whenever intensity of inflammation,the number and /or the size of ulcers have been reduced.Results:The period of time till healing(complete or reduction in size of ulcer), were from 5 to 160 days with mean of 31.2±36.4 days.The mean time of decreasing for secreting,swelling and redness was from 5-11 days.Spearman coefficient of correlation,showed no significant correlation between first size and depth of ulcer and timing till 50%reduction in size of ulcer.Conclusion:All patients had been treated with conventional treatment all to no avail.There were some full thickness ulcer which at the end of treatment we observed an improvement and normal skin for those ulcers.There was a minor complication like itching in one patient but no leading to stop treatment.These findings suggest that AVG is an excellent and safer choice than current conventional therapeutic management of chronic ulcers.
文摘Background: Aloe vera has been used by many civilizations throughout history due to the numerous properties attributed. Gastro oesophageal reflux disease is a common disorder with consequences for the patient’s health related quality of life. Aims: The purpose of this study was to assess the benefits of the Aloe vera in 80 patients with gastro oesophageal reflux. Methods: This is probably the first randomised and double-blind trail ever performed on this subject. The study included eight weeks of treatment with milk enriched with Aloe vera or placebo. Data of diet (frequency questionnaire), symptoms (gastrointestinal symptom rating scale) and quality of life (quality of life in reflux and disease) were obtained. Results: So far, there has been hardly any proof that Aloe vera decreases symptoms of reflux disease;on the contrary, a lower percentage of remission of pain is observed among the Aloe vera group. As long as its effectiveness has not been proven properly, the use of Aloe vera in reflux disease cannot be recommend. Conclusion: Aloe vera does not have a beneficial effect on reflux disease under the study conditions.
