Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration （GTR） and bone grafting. The objective of this study was to investigate the eff...Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration （GTR） and bone grafting. The objective of this study was to investigate the effects of simvastatin/methylcellulose gel on bone regeneration in alveolar defects in miniature pigs. Methods Alveolar defects were produced in 32 teeth （the third and fourth premolars） of 4 miniature pigs. The 32 experimental teeth were divided into 5 groups comprising control （C） and treatment （T） teeth： （1） empty defects without gel （group CO, n=4）; （2） defects injected with methylcellulose gel （group C1, n=4）; （3） defects injected with 0.5 rag/50 pl simvastatin/methylcellulose gel （group T1, n=8）; （4） defects injected with 1.5 mg/50 μl simvastatin/methylcellulose gel （group T2, n=8）; and （5） defects injected with 2.2 mg/50 μl simvastatin/methylcellulose gel （group T3, n=8）. Every week after surgery, the furcation sites were injected once with gel. At the eighth week afte（ surgery, the 4 pigs were sacrificed and underwent macroscopic observation, descriptive histologic examination, and regenerate bone quantitative histologic examination. Results At 8 weeks after surgery, the defect sites in the treatment groups were completely filled in with new bone and fibrous tissue. There was little new bone in the CO and C1 groups, and only a small number of osteoblasts and proliferative vessels could be seen on microscopic examination. Conclusions Miniature pigs are an ideal experimental animal for establishing a model of alveolar defects using a surgical method. Local application of simvastatin/methylcellulose gel can stimulate the regeneration of alveolar bone in furcation defect sites, because it promotes the proliferation of osteoblasts. The best dose of simvastatin gel to stimulate bone regeneration is 0.5 mg.展开更多
Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key step...Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same results.In conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.展开更多
充足的骨量是保证种植体植入和维持长期稳定的关键因素,因而对牙槽骨重度骨缺损的修复重建有着重要的临床意义。随着引导骨再生(guided bone regeneration,GBR)的不断发展,各种GBR术式应用于临床,以解决牙槽骨修复与再生问题。作者基于...充足的骨量是保证种植体植入和维持长期稳定的关键因素,因而对牙槽骨重度骨缺损的修复重建有着重要的临床意义。随着引导骨再生(guided bone regeneration,GBR)的不断发展,各种GBR术式应用于临床,以解决牙槽骨修复与再生问题。作者基于“以稳定性为核心”的牙槽骨修复与再生理念,研发了帐篷钉系列产品,取得了口腔类Ⅲ类医疗器械注册证,并完成了新型帐篷钉技术的构建。该技术能够在不使用自体骨的情况下实现牙槽骨重度缺损的修复与重建,为牙槽骨重度缺损的功能和美学修复提供了新的解决方案。新型帐篷钉技术已在临床上获得了成功验证,并且由于其具有“三低”(技术敏感性低、失败率低及成本低)和“三易”(易操作、易推广及易普及)的特点,使该技术备受口腔医师和患者的青睐。为了进一步提升广大临床医生对新型帐篷钉技术的认识,本文就帐篷钉系列医疗产品的创新性及新型帐篷钉技术的优势作详细介绍,并通过临床病例进行展示,以期该项技术能够在临床上得到规范化应用。展开更多
文摘Currently, the most commonly used treatment methods for repairing alveolar furcation defects are periodontal guided tissue regeneration （GTR） and bone grafting. The objective of this study was to investigate the effects of simvastatin/methylcellulose gel on bone regeneration in alveolar defects in miniature pigs. Methods Alveolar defects were produced in 32 teeth （the third and fourth premolars） of 4 miniature pigs. The 32 experimental teeth were divided into 5 groups comprising control （C） and treatment （T） teeth： （1） empty defects without gel （group CO, n=4）; （2） defects injected with methylcellulose gel （group C1, n=4）; （3） defects injected with 0.5 rag/50 pl simvastatin/methylcellulose gel （group T1, n=8）; （4） defects injected with 1.5 mg/50 μl simvastatin/methylcellulose gel （group T2, n=8）; and （5） defects injected with 2.2 mg/50 μl simvastatin/methylcellulose gel （group T3, n=8）. Every week after surgery, the furcation sites were injected once with gel. At the eighth week afte（ surgery, the 4 pigs were sacrificed and underwent macroscopic observation, descriptive histologic examination, and regenerate bone quantitative histologic examination. Results At 8 weeks after surgery, the defect sites in the treatment groups were completely filled in with new bone and fibrous tissue. There was little new bone in the CO and C1 groups, and only a small number of osteoblasts and proliferative vessels could be seen on microscopic examination. Conclusions Miniature pigs are an ideal experimental animal for establishing a model of alveolar defects using a surgical method. Local application of simvastatin/methylcellulose gel can stimulate the regeneration of alveolar bone in furcation defect sites, because it promotes the proliferation of osteoblasts. The best dose of simvastatin gel to stimulate bone regeneration is 0.5 mg.
基金supported by grants from the Guangzhou Science and Technology Program Key Project(Grant No.201803010044)Guangdong Province College Characteristic Innovation Project(2019KTSCX011)+2 种基金Guangdong Province Natural Sciences Fund Project(2021A1515012480)the Key Areas Research and Development Program of Guangzhou(202103030003)Guangdong Province Special Fund Projects(Yueziranzihe,2021,No.50).
文摘Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same results.In conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.
文摘充足的骨量是保证种植体植入和维持长期稳定的关键因素,因而对牙槽骨重度骨缺损的修复重建有着重要的临床意义。随着引导骨再生(guided bone regeneration,GBR)的不断发展,各种GBR术式应用于临床,以解决牙槽骨修复与再生问题。作者基于“以稳定性为核心”的牙槽骨修复与再生理念,研发了帐篷钉系列产品,取得了口腔类Ⅲ类医疗器械注册证,并完成了新型帐篷钉技术的构建。该技术能够在不使用自体骨的情况下实现牙槽骨重度缺损的修复与重建,为牙槽骨重度缺损的功能和美学修复提供了新的解决方案。新型帐篷钉技术已在临床上获得了成功验证,并且由于其具有“三低”(技术敏感性低、失败率低及成本低)和“三易”(易操作、易推广及易普及)的特点,使该技术备受口腔医师和患者的青睐。为了进一步提升广大临床医生对新型帐篷钉技术的认识,本文就帐篷钉系列医疗产品的创新性及新型帐篷钉技术的优势作详细介绍,并通过临床病例进行展示,以期该项技术能够在临床上得到规范化应用。
