Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaust...Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaustraldehydes A-E(3-7),and three tetra-nor ones ganoaustrenoic acids A-C(8-10).The chemical structures along with the absolute configurations were determined by extensive spectroscopic analysis of 1D&2D NMR and HRESIMS data.The postulated biosynthesis pathways of these compounds were proposed.Ganoaustraldehydes A(3)and B(4)showed moderate inhibition against nitric oxide production in RAW264.7 macrophage cells with the respec-tive IC_(50) values of 32.5,34.2μM(the IC_(50) of positive control pyrrolidine dithiocarbamate was 20.0μM).展开更多
Aurantiadioic acids A(1)and B(2),two new furan-containing polyketides,and aurantoic acid A(3),a new natural product,were isolated from the liquid fermentation of the sika deer dung-derived actinomycete Actinocorallia ...Aurantiadioic acids A(1)and B(2),two new furan-containing polyketides,and aurantoic acid A(3),a new natural product,were isolated from the liquid fermentation of the sika deer dung-derived actinomycete Actinocorallia aurantiaca.The structures of the new compounds were established by extensive spectroscopic methods,including 1D&2D NMR,HRESIMS spectroscopic analysis.The absolute configuration of 3 was assigned by comparison of the specific optical rotations with the reported derivatives.Biological activity evaluations suggested that compounds 1-3 showed weak inhibition on NO production in the murine monocytic RAW 264.7 macrophages with IC_(50)values of 35.8,41.8,45.2μM,respectively.Compound 3 showed weak inhibition on influenza A virus(A/PuertoRico/8/1934,H1N1)with an EC_(50)value of 35.9μM,and a selective index higher than 13.3.展开更多
目的探讨慢性间歇性低压低氧(CIHH)对大鼠胸主动脉环舒张活动的影响及其一氧化氮相关机制。方法成年雄性SD大鼠80只,随机分为对照组(CN组)和慢性间歇性低压低氧组(CIHH组),每组40只。CIHH组给予模拟海拔5 000 m(PB=404 mm Hg,PO2=84 mm ...目的探讨慢性间歇性低压低氧(CIHH)对大鼠胸主动脉环舒张活动的影响及其一氧化氮相关机制。方法成年雄性SD大鼠80只,随机分为对照组(CN组)和慢性间歇性低压低氧组(CIHH组),每组40只。CIHH组给予模拟海拔5 000 m(PB=404 mm Hg,PO2=84 mm Hg)的低压低氧处理,6 h/d,共28 d。对照组处于常压常氧环境,平行饲养。应用离体血管环灌流记录胸主动脉的舒缩活动;采用Western blotting法检测胸主动脉组织中eNOS和PI3K的表达水平。结果与CN组相比,CIHH组乙酰胆碱引起的胸主动脉舒张明显增强(P<0.05),胸主动脉组织中eNOS的表达增多(P<0.05);M EK阻断剂PD98059孵育,对CN组和CIHH组无影响;PI3K阻断剂LY294002孵育,可阻断CIHH组胸主动脉舒张增强和eNOS表达增多(P<0.05);且CIHH组胸主动脉组织中PI3K的表达升高(P<0.05)。结论 CIHH处理可通过PI3K途径活化血管内皮eNOS,增强乙酰胆碱诱导的大鼠胸主动脉舒张。展开更多
基金National Natural Science Foundation of China (grant numbers 21961142008,22177138) for fundings.
文摘Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaustraldehydes A-E(3-7),and three tetra-nor ones ganoaustrenoic acids A-C(8-10).The chemical structures along with the absolute configurations were determined by extensive spectroscopic analysis of 1D&2D NMR and HRESIMS data.The postulated biosynthesis pathways of these compounds were proposed.Ganoaustraldehydes A(3)and B(4)showed moderate inhibition against nitric oxide production in RAW264.7 macrophage cells with the respec-tive IC_(50) values of 32.5,34.2μM(the IC_(50) of positive control pyrrolidine dithiocarbamate was 20.0μM).
基金This work was financially supported by National Natural Science Foundation of China(Grant No.81773590).The authors thank Analytical&Measuring Centre,South-Central University for Nationalities,for the NMR measurements.
文摘Aurantiadioic acids A(1)and B(2),two new furan-containing polyketides,and aurantoic acid A(3),a new natural product,were isolated from the liquid fermentation of the sika deer dung-derived actinomycete Actinocorallia aurantiaca.The structures of the new compounds were established by extensive spectroscopic methods,including 1D&2D NMR,HRESIMS spectroscopic analysis.The absolute configuration of 3 was assigned by comparison of the specific optical rotations with the reported derivatives.Biological activity evaluations suggested that compounds 1-3 showed weak inhibition on NO production in the murine monocytic RAW 264.7 macrophages with IC_(50)values of 35.8,41.8,45.2μM,respectively.Compound 3 showed weak inhibition on influenza A virus(A/PuertoRico/8/1934,H1N1)with an EC_(50)value of 35.9μM,and a selective index higher than 13.3.
