Endophytes,as crucial components of plant microbial communities,significantly contribute to enhancing the absorption of nutrients such as nitrogen and phosphorus by their hosts,promote plant growth,and degrade pathoge...Endophytes,as crucial components of plant microbial communities,significantly contribute to enhancing the absorption of nutrients such as nitrogen and phosphorus by their hosts,promote plant growth,and degrade pathogenic fungal mycelia.In this study,an experiment was conducted in August 2022 to explore the growth-promoting potential of endophytic bacterial strains isolated from two medical plant species,Thymus altaicus and Salvia deserta,using a series of screening media.Plant samples of Thymus altaicus and Salvia deserta were collected from Zhaosu County and Habahe County in Xinjiang Uygur Autonomous Region,China,in July 2021.Additionally,the inhibitory effects of endophytic bacterial strains on the four pathogenic fungi(Fusarium oxysporum,Fulvia fulva,Alternaria solani,and Valsa mali)were determined through the plate confrontation method.A total of 80 endophytic bacterial strains were isolated from Thymus altaicus,while a total of 60 endophytic bacterial strains were isolated from Salvia deserta.The endophytic bacterial strains from both Thymus altaicus and Salvia deserta exhibited plant growth-promoting properties.Specifically,the strains of Bacillus sp.TR002,Bacillus sp.TR005,Microbacterium sp.TSB5,and Rhodococcus sp.TR013 demonstrated strong cellulase-producing activity,siderophore-producing activity,phosphate solubilization activity,and nitrogen-fixing activity,respectively.Out of 140 endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta,104 strains displayed anti-fungal activity against Fulvia fulva,Alternaria solani,Fusarium oxysporum,and Valsa mali.Furthermore,the strains of Bacillus sp.TR005,Bacillus sp.TS003,and Bacillus sp.TSB7 exhibited robust inhibition rates against all the four pathogenic fungi.In conclusion,the endophytic bacterial strains from Thymus altaicus and Salvia deserta possess both plant growth-promoting and anti-fungal properties,making them promising candidates for future development as growth-promoting agents and biocontrol tools for plant diseases.展开更多
Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were c...Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use.The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi(Aspergillus funigaius),yeast(Candida albicans),gram-negative(Acirelobacter haumannii and Pseudornnruis aeruginosa)and gram-positive bacteria(Staphglococcus aureus).The activities were tested at three different concentrations of 1.00,0.10 and 0.01 mg/mL.The data was analysed using Gene data Screener program.Results:The measured antimicrobial activities indicated that out of the 58 plant extracts,15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity.Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities.For instance,Eucommia ulmoides,Pohgonum cuspidcrtum,Poria cocas and Uncaria rhineophylla showed activity against both bacterial and fungal strains,indicating their broad spectrum of activity.Conclusions:The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities.It is interesting to note that the findings from the current study are consistent with the traditional use.A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature.Hence.the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship.This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.展开更多
The anti-fungal activities of synthesized pyridinyl-pyrazole-carbox amide derivatives 1a^1c were investigated via theoretical parameters. The obtained results in present paper were compared with the reported experimen...The anti-fungal activities of synthesized pyridinyl-pyrazole-carbox amide derivatives 1a^1c were investigated via theoretical parameters. The obtained results in present paper were compared with the reported experimental results. Experimental results show that derivative 1a has the highest and derivative 1c the lowest anti-fungal activity. The interactions of derivatives 1a^1c with Ge-nanotube（6, 6） were investigated, and also quantum molecular descriptors of derivatives 1a^1c were calculated. Results show that, derivatives 1a^1c can interact with Ge-nanotube（6, 6） effectively, and their adsorptions were exothermic and possible from the theoretical viewpoint. The absolute ΔEad and ΔGad values of derivative 1a on Ge-nanotube（6, 6） were higher than the absolute ΔEad and ΔGad values of 1 b and 1c. Derivative 1a has the highest absolute μ, ω and ΔN values. The theoretical and experimental trends of anti-fungal activity of derivatives 1a^1c were similar, successfully.展开更多
Dermatophytes were earlier reported to respond well to anti-fungal agents;however, an upsurge in resistance with the high cost of these agents increased the use of medicinal plants for treatment. This study investigat...Dermatophytes were earlier reported to respond well to anti-fungal agents;however, an upsurge in resistance with the high cost of these agents increased the use of medicinal plants for treatment. This study investigated the sensitivity pattern of dermatophytes to oral anti-fungal drugs and aqueous leaf extract of the plant, <em>Acacia nilotica</em>. The extract was tested against seven strains of dermatophytes <em>Arthroderma otae</em>, <em>Trichophyton interdigitale</em>, <em>Trichophyton mentagrophyte</em>, <em>Microsporum ferrugineum</em>, <em>Arthroderma vespertilii</em>, <em>Arthroderma quadrifidum</em>, and <em>Arthroderma multifidum</em>, previously isolated from diabetic patients. The minimum inhibitory and fungicidal concentrations of the plant extracts and the standard antifungal agents were evaluated using modifications of the broth macro dilution method of the National Committee for Clinical Laboratory Standards M38-A2 protocol. There was a significant difference in the Minimum Inhibitory concentrations (MIC) of the dermatophytes to the three antifungal drugs tested (p < 0.001). The dermatophytes were mostly susceptible to itraconazole followed by Nystatin. All the dermatophytes tested were resistant to griseofulvin. <em>Acacia nilotica</em> had an inhibitory effect on all the dermatophytes tested, and showed anti-fungal activity in a dose-dependent relationship between 0.625 - 1.25 mg/ml. Though the inhibitions of the dermatophytes were significantly higher with the standard anti-fungal drugs as compared to the plant extract (p < 0.001);however, the dermatophyte, <em>Arthroderma quadrifidum</em>, which was resistant to all the anti-fungal drugs, had the highest inhibition with <em>A. nilotica</em>. Some circulating dermatophyte strains in Nigeria are griseofulvin and/or itraconazole resistant which may influence the spread of infection and <em>A. nilotica</em> aqueous leaf extract showed a strong anti-dermatophytic activity.展开更多
Microbial interactions between filamentous fungi and yeast are still not fully understood.To evaluate a potential anti-fungal activity of a filamentous fungus while highlighting metabolomic changes,co-cultures between...Microbial interactions between filamentous fungi and yeast are still not fully understood.To evaluate a potential anti-fungal activity of a filamentous fungus while highlighting metabolomic changes,co-cultures between an endophytic strain of Cophinforma mamane(CM)and Candida albicans(CA)were performed.The liquid cultures were incubated under static conditions and metabolite alterations during the course were investigated by ultra-performance liquid chromatography-tandem mass spectrophotometry(UPLC-MS/MS).Results were analyzed using MS-DIAL,MS-FINDER,METLIN,Xcalibur,SciFinder,and MetaboAnalyst metabolomics platforms.The metabolites associated with catabolic processes,including the metabolism of branched-chain amino acids,carnitine,and phospholipids were upregulated both in the mono and co-cultures,indicating fungal adaptability to environmental stress.Several metab-olites,including C20 sphinganine 1-phosphate,myo-inositol,farnesol,gamma-undecalactone,folinic acid,palmitoleic acid,and MG(12:/0:0/0:0)were not produced by CA during co-culture with CM,demonstrating the antifungal mecha-nism of CM.Our results highlight the crucial roles of metabolomics studies to provide essential information regarding the antifungal mechanism of C.mamane against C.albicans,especially when the lost/undetected metabolites are involved in fungal survival and pathogenicity.展开更多
Highly efficient silver halide nanoparticles (AgX, X = Cl, Br NP’s) were successfully synthesized by facile and template-free direct-precipitation method using potassium chloride, potassium bromide and silver nitrate...Highly efficient silver halide nanoparticles (AgX, X = Cl, Br NP’s) were successfully synthesized by facile and template-free direct-precipitation method using potassium chloride, potassium bromide and silver nitrate as reactive sources. The as-prepared AgX NP’s were characterized by FTIR, thermogravimetric analysis, XRD, EDX and HRTEM. The antimicrobial susceptibilities against two Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus) and four Gram-negative bacteria (Salmonella typhi, Escherichia coli, P. aeruginosa and Klebsiella pneumoniae) in addition to five fungi (Aspergillus flavus, A. carbonarus, Penicillium verrucosum, Fusarium verticelloides and A. niger) were tested by the disk diffusion technique. The antibacterial and antifungal results suggest that the prepared AgX NP’s show high activity against the tested organisms compared to tetracycline and Nystatin taken as standard drugs. As an application, the use of the prepared AgX NP’s as photo catalyst for the decontamination of malathion as VX chemical warfare agent (CWA) stimulant from water sample was extensively studied.展开更多
Highly efficient silver halide nanoparticles (AgX, X = Cl, Br NP’s) were successfully synthesized by facile and template-free direct-precipitation method using potassium chloride, potassium bromide and silver nitrate...Highly efficient silver halide nanoparticles (AgX, X = Cl, Br NP’s) were successfully synthesized by facile and template-free direct-precipitation method using potassium chloride, potassium bromide and silver nitrate as reactive sources. The as-prepared AgX NP’s were characterized by FTIR, thermogravimetric analysis, XRD, EDX and HRTEM. The antimicrobial susceptibilities against two Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus) and four Gram-negative bacteria (Salmonella typhi, Escherichia coli, P. aeruginosa and Klebsiella pneumoniae) in addition to five fungi (Aspergillus flavus, A. carbonarus, Penicillium verrucosum, Fusarium verticelloides and A. niger) were tested by the disk diffusion technique. The antibacterial and antifungal results suggest that the prepared AgX NP’s show high activity against the tested organisms compared to tetracycline and Nystatin taken as standard drugs. As an application, the use of the prepared AgX NP’s as photo catalyst for the decontamination of malathion as VX chemical warfare agent (CWA) stimulant from water sample was extensively studied.展开更多
基金financially supported by the Third Xinjiang Comprehensive Scientific Expedition (2022xjkk020605)the Xinjiang Uygur Autonomous Region Regional Coordinated Innovation Project (Shanghai Cooperation Organization Science and Technology Partnership Program) (2020E01047)supported by the Introduction Project of High-level Talents in Xinjiang Uygur Autonomous Region, China
文摘Endophytes,as crucial components of plant microbial communities,significantly contribute to enhancing the absorption of nutrients such as nitrogen and phosphorus by their hosts,promote plant growth,and degrade pathogenic fungal mycelia.In this study,an experiment was conducted in August 2022 to explore the growth-promoting potential of endophytic bacterial strains isolated from two medical plant species,Thymus altaicus and Salvia deserta,using a series of screening media.Plant samples of Thymus altaicus and Salvia deserta were collected from Zhaosu County and Habahe County in Xinjiang Uygur Autonomous Region,China,in July 2021.Additionally,the inhibitory effects of endophytic bacterial strains on the four pathogenic fungi(Fusarium oxysporum,Fulvia fulva,Alternaria solani,and Valsa mali)were determined through the plate confrontation method.A total of 80 endophytic bacterial strains were isolated from Thymus altaicus,while a total of 60 endophytic bacterial strains were isolated from Salvia deserta.The endophytic bacterial strains from both Thymus altaicus and Salvia deserta exhibited plant growth-promoting properties.Specifically,the strains of Bacillus sp.TR002,Bacillus sp.TR005,Microbacterium sp.TSB5,and Rhodococcus sp.TR013 demonstrated strong cellulase-producing activity,siderophore-producing activity,phosphate solubilization activity,and nitrogen-fixing activity,respectively.Out of 140 endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta,104 strains displayed anti-fungal activity against Fulvia fulva,Alternaria solani,Fusarium oxysporum,and Valsa mali.Furthermore,the strains of Bacillus sp.TR005,Bacillus sp.TS003,and Bacillus sp.TSB7 exhibited robust inhibition rates against all the four pathogenic fungi.In conclusion,the endophytic bacterial strains from Thymus altaicus and Salvia deserta possess both plant growth-promoting and anti-fungal properties,making them promising candidates for future development as growth-promoting agents and biocontrol tools for plant diseases.
基金funded by the University of Western Sydney and the Fundacion MEDINAa public-private partnership of Merck Sharp&Dohme de Espana S.A./Universidad de Granada/Junta de Andalucia
文摘Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use.The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi(Aspergillus funigaius),yeast(Candida albicans),gram-negative(Acirelobacter haumannii and Pseudornnruis aeruginosa)and gram-positive bacteria(Staphglococcus aureus).The activities were tested at three different concentrations of 1.00,0.10 and 0.01 mg/mL.The data was analysed using Gene data Screener program.Results:The measured antimicrobial activities indicated that out of the 58 plant extracts,15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity.Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities.For instance,Eucommia ulmoides,Pohgonum cuspidcrtum,Poria cocas and Uncaria rhineophylla showed activity against both bacterial and fungal strains,indicating their broad spectrum of activity.Conclusions:The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities.It is interesting to note that the findings from the current study are consistent with the traditional use.A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature.Hence.the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship.This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.
文摘The anti-fungal activities of synthesized pyridinyl-pyrazole-carbox amide derivatives 1a^1c were investigated via theoretical parameters. The obtained results in present paper were compared with the reported experimental results. Experimental results show that derivative 1a has the highest and derivative 1c the lowest anti-fungal activity. The interactions of derivatives 1a^1c with Ge-nanotube（6, 6） were investigated, and also quantum molecular descriptors of derivatives 1a^1c were calculated. Results show that, derivatives 1a^1c can interact with Ge-nanotube（6, 6） effectively, and their adsorptions were exothermic and possible from the theoretical viewpoint. The absolute ΔEad and ΔGad values of derivative 1a on Ge-nanotube（6, 6） were higher than the absolute ΔEad and ΔGad values of 1 b and 1c. Derivative 1a has the highest absolute μ, ω and ΔN values. The theoretical and experimental trends of anti-fungal activity of derivatives 1a^1c were similar, successfully.
文摘Dermatophytes were earlier reported to respond well to anti-fungal agents;however, an upsurge in resistance with the high cost of these agents increased the use of medicinal plants for treatment. This study investigated the sensitivity pattern of dermatophytes to oral anti-fungal drugs and aqueous leaf extract of the plant, <em>Acacia nilotica</em>. The extract was tested against seven strains of dermatophytes <em>Arthroderma otae</em>, <em>Trichophyton interdigitale</em>, <em>Trichophyton mentagrophyte</em>, <em>Microsporum ferrugineum</em>, <em>Arthroderma vespertilii</em>, <em>Arthroderma quadrifidum</em>, and <em>Arthroderma multifidum</em>, previously isolated from diabetic patients. The minimum inhibitory and fungicidal concentrations of the plant extracts and the standard antifungal agents were evaluated using modifications of the broth macro dilution method of the National Committee for Clinical Laboratory Standards M38-A2 protocol. There was a significant difference in the Minimum Inhibitory concentrations (MIC) of the dermatophytes to the three antifungal drugs tested (p < 0.001). The dermatophytes were mostly susceptible to itraconazole followed by Nystatin. All the dermatophytes tested were resistant to griseofulvin. <em>Acacia nilotica</em> had an inhibitory effect on all the dermatophytes tested, and showed anti-fungal activity in a dose-dependent relationship between 0.625 - 1.25 mg/ml. Though the inhibitions of the dermatophytes were significantly higher with the standard anti-fungal drugs as compared to the plant extract (p < 0.001);however, the dermatophyte, <em>Arthroderma quadrifidum</em>, which was resistant to all the anti-fungal drugs, had the highest inhibition with <em>A. nilotica</em>. Some circulating dermatophyte strains in Nigeria are griseofulvin and/or itraconazole resistant which may influence the spread of infection and <em>A. nilotica</em> aqueous leaf extract showed a strong anti-dermatophytic activity.
文摘Microbial interactions between filamentous fungi and yeast are still not fully understood.To evaluate a potential anti-fungal activity of a filamentous fungus while highlighting metabolomic changes,co-cultures between an endophytic strain of Cophinforma mamane(CM)and Candida albicans(CA)were performed.The liquid cultures were incubated under static conditions and metabolite alterations during the course were investigated by ultra-performance liquid chromatography-tandem mass spectrophotometry(UPLC-MS/MS).Results were analyzed using MS-DIAL,MS-FINDER,METLIN,Xcalibur,SciFinder,and MetaboAnalyst metabolomics platforms.The metabolites associated with catabolic processes,including the metabolism of branched-chain amino acids,carnitine,and phospholipids were upregulated both in the mono and co-cultures,indicating fungal adaptability to environmental stress.Several metab-olites,including C20 sphinganine 1-phosphate,myo-inositol,farnesol,gamma-undecalactone,folinic acid,palmitoleic acid,and MG(12:/0:0/0:0)were not produced by CA during co-culture with CM,demonstrating the antifungal mecha-nism of CM.Our results highlight the crucial roles of metabolomics studies to provide essential information regarding the antifungal mechanism of C.mamane against C.albicans,especially when the lost/undetected metabolites are involved in fungal survival and pathogenicity.
文摘Highly efficient silver halide nanoparticles (AgX, X = Cl, Br NP’s) were successfully synthesized by facile and template-free direct-precipitation method using potassium chloride, potassium bromide and silver nitrate as reactive sources. The as-prepared AgX NP’s were characterized by FTIR, thermogravimetric analysis, XRD, EDX and HRTEM. The antimicrobial susceptibilities against two Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus) and four Gram-negative bacteria (Salmonella typhi, Escherichia coli, P. aeruginosa and Klebsiella pneumoniae) in addition to five fungi (Aspergillus flavus, A. carbonarus, Penicillium verrucosum, Fusarium verticelloides and A. niger) were tested by the disk diffusion technique. The antibacterial and antifungal results suggest that the prepared AgX NP’s show high activity against the tested organisms compared to tetracycline and Nystatin taken as standard drugs. As an application, the use of the prepared AgX NP’s as photo catalyst for the decontamination of malathion as VX chemical warfare agent (CWA) stimulant from water sample was extensively studied.
文摘Highly efficient silver halide nanoparticles (AgX, X = Cl, Br NP’s) were successfully synthesized by facile and template-free direct-precipitation method using potassium chloride, potassium bromide and silver nitrate as reactive sources. The as-prepared AgX NP’s were characterized by FTIR, thermogravimetric analysis, XRD, EDX and HRTEM. The antimicrobial susceptibilities against two Gram-positive bacteria (Bacillus cereus, Staphylococcus aureus) and four Gram-negative bacteria (Salmonella typhi, Escherichia coli, P. aeruginosa and Klebsiella pneumoniae) in addition to five fungi (Aspergillus flavus, A. carbonarus, Penicillium verrucosum, Fusarium verticelloides and A. niger) were tested by the disk diffusion technique. The antibacterial and antifungal results suggest that the prepared AgX NP’s show high activity against the tested organisms compared to tetracycline and Nystatin taken as standard drugs. As an application, the use of the prepared AgX NP’s as photo catalyst for the decontamination of malathion as VX chemical warfare agent (CWA) stimulant from water sample was extensively studied.
