Objective:To verify through a scientific mean the therapeutic use of the plant as an anti-tussive agent,by traditional medicine healers and also aid in the search for new drugs developments from plants.Methods:The roo...Objective:To verify through a scientific mean the therapeutic use of the plant as an anti-tussive agent,by traditional medicine healers and also aid in the search for new drugs developments from plants.Methods:The roots of Triclisia dictyophylla were investigated for their antitussive properties.The plant was uprooted in the month of June 2003 at Igbodo,Delta State,Nigeria and was taken to the university of Nigeria Nsukka for taxonomy. The roots were chopped,ground and immersed in pure drinking water for 24 hours.After filtration, extraction was carried out using a Rotary evaporator,preliminary phytochemistry and acute toxicity studies were carried out.Antitussive study was carried out using a total of 42 young rats of average weight of 72.2 g.The rats were housed in standard animal house of the university and were allowed access to feeds and water but, were fasted for 12 hours prior to commencement of experiment.Specific and appropriate dosage of the crude extract and Codeine re - dissolved in water were administered orally 30 minutes prior to induction of cough. Cough was induced by exposing the animals to Sulphure dioxide gas for 3- minutes.Coughing was taken as number of Head-nods per minute,Stethoscope aided audible sounds and,or tears secretion.Percentage cough inhibition for crude extract and,or Codeine treated rats were compared with reference to control animals.Results were subjected to statistical analysis using SPSS 13.0.Results:A 10.2%extraction yield was got from a starting root initial weight of 320 g.The preliminary phytochemistry of the aqueous root extract revealed the presence of alkaloids,saponins,flavonoids,proteins,reducing sugars,steroids,resins fats/oils and glycosides. The Median lethal dose(LD_(50)) based on Lorke's 1983 method was 548 mg/kg The aqueous root extract at concentrations of 10 mg/kg,50 mg/kg,100 mg/kg and 200 mg/kg orally administered,inhibited cough in rats induced by sulphure dioxide gas by 16.67%,33.33%,50.00%and 83.33%respectively.While Codeine phosphate,a standard antitussive agent,at oral concentrations of 10 mg/kg and 20 mg/kg inhibited cough in rats induced by sulphure dioxide gas by 33.33%and 60.67%respectively.Conclusion:Earlier works by some authors had led to isolation of Morhinian Alkaloids from Triclisia dictyophylla thus most probably linking its mechanism of antitussive activity to that likable of Morphine.This study justifies the use of the plant in treatment of cough by Traditional Medicine Healers.展开更多
Objective:To evaluate the antiasthmatic and antitussive properties of the aqueous leaf extract of Bryophyilum pinnatum {B.pinnatum)(BP) Lam.Methods:Ovalbiunin-sensilized guinea pigs which were treated with BP for 21 c...Objective:To evaluate the antiasthmatic and antitussive properties of the aqueous leaf extract of Bryophyilum pinnatum {B.pinnatum)(BP) Lam.Methods:Ovalbiunin-sensilized guinea pigs which were treated with BP for 21 consecutive days were exposed to 0.2%histamine aerosol in a glass chamber.Mucus viscosity,white blood cell and lymphocyte counts and tracheal wall morphometry were measured.Bouts of cough were counted pre and post acute exposure of extract-treated(X7 d) guinea pigs to 7.5%citric acid aerosol in a chamber.Phenol red expectoration was estimated in mice after 7 d of daily administration of BP.Results:Doses of 200 and 400 mg/kg/day(×21 d) BP significantly increased the time for guinea pigs to experience preconvulsive dyspnoea.BP and salbutamol(0.5 mg/kg/day×21 d) reduced mucus viscosity in the sensitized group to values comparable with controls.White blood cell,lymphocyte counts and tracheal morphometry were not significantly altered.Both doses of BP also significantly reduced the bouts of cough but only 400 mg/kg/day significantly inhibited the amount of phenol red secreted.Conclusions:BP has demonstrated antiasthmatic and antitussive properties in these rodent models.These properties may underscore its use in Nigerian ethnomedicine.展开更多
The title compound 9 S,9 aS-neotuberostemonine(1) was isolated from the 95% ethanol extract of the roots of Stemona tuberosa. The crystal structure of 1, C_922)H_(33)NO_4, was determined by single-crystal X-ray diffra...The title compound 9 S,9 aS-neotuberostemonine(1) was isolated from the 95% ethanol extract of the roots of Stemona tuberosa. The crystal structure of 1, C_922)H_(33)NO_4, was determined by single-crystal X-ray diffraction analysis. The crystal belongs to orthorhombic system, space group P212121, with a = 9.0115(11), b = 10.612(4), c = 22.074(3) ?, V = 2110.9(8) ?~3, Z = 4, Mr = 375.49, Dc = 1.182 g/cm3, λ= 0.71079 ?, μ = 0.080 cm-1, F(000) = 816, S = 1.019, R = 0.0579 and w R = 0.1358. A total of 3109 unique reflections were collected, of which 2902 were observed(I > 2σ(I)). The absolute configuration of 1 could be assigned by referring to the conserved configuration of the methyl groups at C(13) and C(20). In the solid state, the molecules were linked into a chain along the a-axis through weak hydrogen bond C(11)–H(11 A)…O(2). Compound 1 shows significant inhibition of cough by 24%, 44% and 65% at doses of 50, 100 and 150 mg/kg, respectively.展开更多
[Objectives]This study was conducted to investigate the antitussive and anti-inflammatory effects of Miao medicine Aspidistra caespitosa C.Pei.[Methods]A mouse cough model was made by the SO 2 cough induction method,a...[Objectives]This study was conducted to investigate the antitussive and anti-inflammatory effects of Miao medicine Aspidistra caespitosa C.Pei.[Methods]A mouse cough model was made by the SO 2 cough induction method,and the antitussive effects of different extraction fractions of A.caespitosa were observed.The inflammation models of acute inflammatory ear swelling in mice caused by xylene,granuloma in mice caused by cotton balls,and footpad swelling in mice caused by carrageenan were made,and the different extraction fractions were used to observe the effects of different extraction fractions on mouse ear swelling,granuloma and footpad swelling.And the levels of interleukin 6(IL-6)and tumor necrosis factor(TNF-a)in the serum of different groups of mice were detected to screen out the extraction fractions of A.caespitosa with good antitussive and anti-inflammatory activity.[Results]Antitussive experiments on mice showed that the water layer extraction fraction,ethyl acetate extraction fraction and petroleum ether extraction fraction of Miao medicine A.caespitosa could prolong the incubation period of cough in mice and reduce the number of coughs within 2 min to varying degrees,and its different extraction fractions all had different inhibitory effects on mouse ear swelling,granuloma and footpad swelling.Compared with the blank control group,the different extraction fractions could reduce IL-6,TNF-a and other indicators to different degrees.[Conclusions]Ethyl acetate extraction fraction of A.caespitosa has good antitussive and anti-inflammatory effects.展开更多
文摘Objective:To verify through a scientific mean the therapeutic use of the plant as an anti-tussive agent,by traditional medicine healers and also aid in the search for new drugs developments from plants.Methods:The roots of Triclisia dictyophylla were investigated for their antitussive properties.The plant was uprooted in the month of June 2003 at Igbodo,Delta State,Nigeria and was taken to the university of Nigeria Nsukka for taxonomy. The roots were chopped,ground and immersed in pure drinking water for 24 hours.After filtration, extraction was carried out using a Rotary evaporator,preliminary phytochemistry and acute toxicity studies were carried out.Antitussive study was carried out using a total of 42 young rats of average weight of 72.2 g.The rats were housed in standard animal house of the university and were allowed access to feeds and water but, were fasted for 12 hours prior to commencement of experiment.Specific and appropriate dosage of the crude extract and Codeine re - dissolved in water were administered orally 30 minutes prior to induction of cough. Cough was induced by exposing the animals to Sulphure dioxide gas for 3- minutes.Coughing was taken as number of Head-nods per minute,Stethoscope aided audible sounds and,or tears secretion.Percentage cough inhibition for crude extract and,or Codeine treated rats were compared with reference to control animals.Results were subjected to statistical analysis using SPSS 13.0.Results:A 10.2%extraction yield was got from a starting root initial weight of 320 g.The preliminary phytochemistry of the aqueous root extract revealed the presence of alkaloids,saponins,flavonoids,proteins,reducing sugars,steroids,resins fats/oils and glycosides. The Median lethal dose(LD_(50)) based on Lorke's 1983 method was 548 mg/kg The aqueous root extract at concentrations of 10 mg/kg,50 mg/kg,100 mg/kg and 200 mg/kg orally administered,inhibited cough in rats induced by sulphure dioxide gas by 16.67%,33.33%,50.00%and 83.33%respectively.While Codeine phosphate,a standard antitussive agent,at oral concentrations of 10 mg/kg and 20 mg/kg inhibited cough in rats induced by sulphure dioxide gas by 33.33%and 60.67%respectively.Conclusion:Earlier works by some authors had led to isolation of Morhinian Alkaloids from Triclisia dictyophylla thus most probably linking its mechanism of antitussive activity to that likable of Morphine.This study justifies the use of the plant in treatment of cough by Traditional Medicine Healers.
文摘Objective:To evaluate the antiasthmatic and antitussive properties of the aqueous leaf extract of Bryophyilum pinnatum {B.pinnatum)(BP) Lam.Methods:Ovalbiunin-sensilized guinea pigs which were treated with BP for 21 consecutive days were exposed to 0.2%histamine aerosol in a glass chamber.Mucus viscosity,white blood cell and lymphocyte counts and tracheal wall morphometry were measured.Bouts of cough were counted pre and post acute exposure of extract-treated(X7 d) guinea pigs to 7.5%citric acid aerosol in a chamber.Phenol red expectoration was estimated in mice after 7 d of daily administration of BP.Results:Doses of 200 and 400 mg/kg/day(×21 d) BP significantly increased the time for guinea pigs to experience preconvulsive dyspnoea.BP and salbutamol(0.5 mg/kg/day×21 d) reduced mucus viscosity in the sensitized group to values comparable with controls.White blood cell,lymphocyte counts and tracheal morphometry were not significantly altered.Both doses of BP also significantly reduced the bouts of cough but only 400 mg/kg/day significantly inhibited the amount of phenol red secreted.Conclusions:BP has demonstrated antiasthmatic and antitussive properties in these rodent models.These properties may underscore its use in Nigerian ethnomedicine.
基金supported by Guangdong Key Scientific Project(2013A022100029)Zhongshan Scientific Scheme(2017B1134)
文摘The title compound 9 S,9 aS-neotuberostemonine(1) was isolated from the 95% ethanol extract of the roots of Stemona tuberosa. The crystal structure of 1, C_922)H_(33)NO_4, was determined by single-crystal X-ray diffraction analysis. The crystal belongs to orthorhombic system, space group P212121, with a = 9.0115(11), b = 10.612(4), c = 22.074(3) ?, V = 2110.9(8) ?~3, Z = 4, Mr = 375.49, Dc = 1.182 g/cm3, λ= 0.71079 ?, μ = 0.080 cm-1, F(000) = 816, S = 1.019, R = 0.0579 and w R = 0.1358. A total of 3109 unique reflections were collected, of which 2902 were observed(I > 2σ(I)). The absolute configuration of 1 could be assigned by referring to the conserved configuration of the methyl groups at C(13) and C(20). In the solid state, the molecules were linked into a chain along the a-axis through weak hydrogen bond C(11)–H(11 A)…O(2). Compound 1 shows significant inhibition of cough by 24%, 44% and 65% at doses of 50, 100 and 150 mg/kg, respectively.
基金Scientific and Technological Research Project of Traditional Chinese Medicine and Ethnic Medicine of Guizhou Administration of Traditional Chinese Medicine(QZYY2017-116)Scientific Research Project of Guizhou University of Traditional Chinese Medicine(GZYKYN[2017]28).
文摘[Objectives]This study was conducted to investigate the antitussive and anti-inflammatory effects of Miao medicine Aspidistra caespitosa C.Pei.[Methods]A mouse cough model was made by the SO 2 cough induction method,and the antitussive effects of different extraction fractions of A.caespitosa were observed.The inflammation models of acute inflammatory ear swelling in mice caused by xylene,granuloma in mice caused by cotton balls,and footpad swelling in mice caused by carrageenan were made,and the different extraction fractions were used to observe the effects of different extraction fractions on mouse ear swelling,granuloma and footpad swelling.And the levels of interleukin 6(IL-6)and tumor necrosis factor(TNF-a)in the serum of different groups of mice were detected to screen out the extraction fractions of A.caespitosa with good antitussive and anti-inflammatory activity.[Results]Antitussive experiments on mice showed that the water layer extraction fraction,ethyl acetate extraction fraction and petroleum ether extraction fraction of Miao medicine A.caespitosa could prolong the incubation period of cough in mice and reduce the number of coughs within 2 min to varying degrees,and its different extraction fractions all had different inhibitory effects on mouse ear swelling,granuloma and footpad swelling.Compared with the blank control group,the different extraction fractions could reduce IL-6,TNF-a and other indicators to different degrees.[Conclusions]Ethyl acetate extraction fraction of A.caespitosa has good antitussive and anti-inflammatory effects.
