●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in spe...●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing eye-related diseases for further studies.展开更多
采用二维聚丙烯酰胺凝胶电泳(2D-PAGE)分析妊娠早期胚胎绒毛和子宫蜕膜组织细胞的蛋白质组。结果发现一个等电点约6.6、分子量约38kDa的蛋白质点在蜕膜组织中表达明显下调,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内...采用二维聚丙烯酰胺凝胶电泳(2D-PAGE)分析妊娠早期胚胎绒毛和子宫蜕膜组织细胞的蛋白质组。结果发现一个等电点约6.6、分子量约38kDa的蛋白质点在蜕膜组织中表达明显下调,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质量指纹谱(peptide mass fingerprint,PMF)和数据库搜索鉴定此蛋白质点为膜联蛋白-A1(ANXA1)。进一步采用RT-PCR、Western印迹和免疫组织化学技术分析了ANXA1在胚胎绒毛和子宫蜕膜组织细胞中的表达情况,证实了ANXA1 mRNA水平及其蛋白质在蜕膜组织细胞中呈现低水平表达,实验结果提示ANXA1可能在滋养细胞浸润和胎盘形成过程中发挥重要作用。展开更多
基金Supported by Regional Science Foundation Project of the National Natural Science Foundation of China(No.82060827,No.82260891)The Key Discipline of Universities in the“14th Five-Year Plan”Autonomous Region-Traditional Chinese Medicine at Xinjiang Medical University.
文摘●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing eye-related diseases for further studies.
文摘采用二维聚丙烯酰胺凝胶电泳(2D-PAGE)分析妊娠早期胚胎绒毛和子宫蜕膜组织细胞的蛋白质组。结果发现一个等电点约6.6、分子量约38kDa的蛋白质点在蜕膜组织中表达明显下调,经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质量指纹谱(peptide mass fingerprint,PMF)和数据库搜索鉴定此蛋白质点为膜联蛋白-A1(ANXA1)。进一步采用RT-PCR、Western印迹和免疫组织化学技术分析了ANXA1在胚胎绒毛和子宫蜕膜组织细胞中的表达情况,证实了ANXA1 mRNA水平及其蛋白质在蜕膜组织细胞中呈现低水平表达,实验结果提示ANXA1可能在滋养细胞浸润和胎盘形成过程中发挥重要作用。
