[Objective] This study presents a method for mass rearing of Aphidius gifuensis, a dominant endoparasitoid of Myzus persicae on tobacco in Southwest China. [Method] The tobacco cultivar Honghuadajinyuan(Nicotiana taba...[Objective] This study presents a method for mass rearing of Aphidius gifuensis, a dominant endoparasitoid of Myzus persicae on tobacco in Southwest China. [Method] The tobacco cultivar Honghuadajinyuan(Nicotiana tabacum) was used as the host plant and M. persicae was the host insect. In a greenhouse, tobacco seedlings were reared in plastic trays. The seedlings at three-true-leaf stage were inoculated with two to three aphids per plant using aphid source with a parasitism rate of 47% ±3.9%. [Result] By this inoculation method, the aphids and parasites were simultaneously inoculated on host plants. After approximately 25 d of rearing, we were able to produce 82.5±5.17 aphid mummies per tobacco seedling. A total of 445 500 aphid mummies were produced in one greenhouse(36 rearing trays per greenhouse) during an approximately 50-day rearing period. The emergence rate was 93.4% ±2.76%, and 54% of the mummies were females. [Conclusion] The demonstrated technological feasibility of using tobacco seedlings for the mass rearing of A. gifuensis increases the potential for the biological control of M. persicae.展开更多
The prevention and control effects of Aphidius gifuensis against Myzus persicae in four major tobacco planting villages and towns in Shuicheng County were investigated from 2015 to 2016. The results showed that the pa...The prevention and control effects of Aphidius gifuensis against Myzus persicae in four major tobacco planting villages and towns in Shuicheng County were investigated from 2015 to 2016. The results showed that the parasitic rate of M. persicae before A. gifuensis release was 0. 85% in field,and that at 20 d after A. gifuensis release was 59. 49%. The incidence rate of aphid-transmitted virus disease was controlled at 0. 62%. The release of A. gifuensis increased its natural population quantity in field,and had obvious direct control effects against M. persicae. Meantime,it indirectly controlled the occurrence of aphid-transmitted virus disease to some extent.展开更多
Aphidius gifuensis Ashmead is an economically important aphid parasitoid,particularly for pest Myzus persicae.The aim of the study was to feed M.persicae using a formulated artificial medium instead of tobacco to main...Aphidius gifuensis Ashmead is an economically important aphid parasitoid,particularly for pest Myzus persicae.The aim of the study was to feed M.persicae using a formulated artificial medium instead of tobacco to maintain parasitoid A.gifuensis in winter.The development,survival and reproduction of M.persicae fed with four artificial media(AM1-AM4),as well as the parasitism,emergence and longevity of A.gifuensis when reared on F3 M.persicae fed with AMI,were assessed.Over 15 days of the study,the survival rates of A.gifuensis for the AM1 and AM2 treatments were significantly greater than those for the AM3 and AM4 treatments.The highest fecundity of F,A.gifuensis females was observed on AM1 feeding.No differences were noted in survival rate,development or nymph production between F:and F,M.persicae fed with AM1.No differences were recorded in the number of mummies,parasitism rate,adult Iongevity or proportion of A.gifuensis females between Treatment I(4.gifuensis parasitizing AM1-reared M.persicae)and the control.However,the emergence rate of Treatment I was lower than that of the control.The rearing of.A.gifuensis on M.persicae fed with an artificial medium was found to be a feasible proposition.This could save time,space and cost compared with traditional production methods.展开更多
基金Supported by Yunnan Provincial Tobacco Corporation(09YN015,2012YN14)
文摘[Objective] This study presents a method for mass rearing of Aphidius gifuensis, a dominant endoparasitoid of Myzus persicae on tobacco in Southwest China. [Method] The tobacco cultivar Honghuadajinyuan(Nicotiana tabacum) was used as the host plant and M. persicae was the host insect. In a greenhouse, tobacco seedlings were reared in plastic trays. The seedlings at three-true-leaf stage were inoculated with two to three aphids per plant using aphid source with a parasitism rate of 47% ±3.9%. [Result] By this inoculation method, the aphids and parasites were simultaneously inoculated on host plants. After approximately 25 d of rearing, we were able to produce 82.5±5.17 aphid mummies per tobacco seedling. A total of 445 500 aphid mummies were produced in one greenhouse(36 rearing trays per greenhouse) during an approximately 50-day rearing period. The emergence rate was 93.4% ±2.76%, and 54% of the mummies were females. [Conclusion] The demonstrated technological feasibility of using tobacco seedlings for the mass rearing of A. gifuensis increases the potential for the biological control of M. persicae.
文摘The prevention and control effects of Aphidius gifuensis against Myzus persicae in four major tobacco planting villages and towns in Shuicheng County were investigated from 2015 to 2016. The results showed that the parasitic rate of M. persicae before A. gifuensis release was 0. 85% in field,and that at 20 d after A. gifuensis release was 59. 49%. The incidence rate of aphid-transmitted virus disease was controlled at 0. 62%. The release of A. gifuensis increased its natural population quantity in field,and had obvious direct control effects against M. persicae. Meantime,it indirectly controlled the occurrence of aphid-transmitted virus disease to some extent.
文摘Aphidius gifuensis Ashmead is an economically important aphid parasitoid,particularly for pest Myzus persicae.The aim of the study was to feed M.persicae using a formulated artificial medium instead of tobacco to maintain parasitoid A.gifuensis in winter.The development,survival and reproduction of M.persicae fed with four artificial media(AM1-AM4),as well as the parasitism,emergence and longevity of A.gifuensis when reared on F3 M.persicae fed with AMI,were assessed.Over 15 days of the study,the survival rates of A.gifuensis for the AM1 and AM2 treatments were significantly greater than those for the AM3 and AM4 treatments.The highest fecundity of F,A.gifuensis females was observed on AM1 feeding.No differences were noted in survival rate,development or nymph production between F:and F,M.persicae fed with AM1.No differences were recorded in the number of mummies,parasitism rate,adult Iongevity or proportion of A.gifuensis females between Treatment I(4.gifuensis parasitizing AM1-reared M.persicae)and the control.However,the emergence rate of Treatment I was lower than that of the control.The rearing of.A.gifuensis on M.persicae fed with an artificial medium was found to be a feasible proposition.This could save time,space and cost compared with traditional production methods.
