[Objective] The purpose of this experiment was to reveal the sequence and characteristics of Trachidermus fasciatus beta-actin gene.[Method] Using total RNA of muscle in T.fasciatus as template,three cDNA fragments of...[Objective] The purpose of this experiment was to reveal the sequence and characteristics of Trachidermus fasciatus beta-actin gene.[Method] Using total RNA of muscle in T.fasciatus as template,three cDNA fragments of beta-actin gene in T.fasciatus were amplified by RT-PCR,5＇-RACE and 3＇-RACE.[Result] A full-length of 1905 bp beta-actin cDNA sequence in T.fasciatus,which includes a 1128 bp length open reading frame encoding a 375-amino acid peptide,was obtained.Sequence alignment of nucleotide and amino acid sequence revealed that T.fasciatus beta-actin shared high homology with that of Epinephelus coioides,Rachycentron canadum,Chrysophrys auratus and of relatively low homology with mammalian and bird.The phylogenetic analysis showed that T.fasciatus beta-actin had closest relationship with Epinephelus coioides.And RT-PCR analysis suggested that the beta-actin gene expressed in four tissues,i.e.,muscle,liver,intestine and brain.[Conclusion] The full length sequence of beta-actin gene with high conservation in T.fasciatus was obtained for the first time.展开更多
基金Supported by Natural Science Research Program of Universities in Jiangsu Province(09KJD240001)Agricultural Science and Technology R&D Program of Suzhou City(SNG0913)~~
文摘[Objective] The purpose of this experiment was to reveal the sequence and characteristics of Trachidermus fasciatus beta-actin gene.[Method] Using total RNA of muscle in T.fasciatus as template,three cDNA fragments of beta-actin gene in T.fasciatus were amplified by RT-PCR,5＇-RACE and 3＇-RACE.[Result] A full-length of 1905 bp beta-actin cDNA sequence in T.fasciatus,which includes a 1128 bp length open reading frame encoding a 375-amino acid peptide,was obtained.Sequence alignment of nucleotide and amino acid sequence revealed that T.fasciatus beta-actin shared high homology with that of Epinephelus coioides,Rachycentron canadum,Chrysophrys auratus and of relatively low homology with mammalian and bird.The phylogenetic analysis showed that T.fasciatus beta-actin had closest relationship with Epinephelus coioides.And RT-PCR analysis suggested that the beta-actin gene expressed in four tissues,i.e.,muscle,liver,intestine and brain.[Conclusion] The full length sequence of beta-actin gene with high conservation in T.fasciatus was obtained for the first time.
