Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical functions.However,the diversity of plant materials makes the plant derived ELN study challenging.More efforts are sti...Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical functions.However,the diversity of plant materials makes the plant derived ELN study challenging.More efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human health.In this study,a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs(B-ELNs),and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing.A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue,and their potential cross-kingdom human gene targets were further predicted.Results showed that size exclusion chromatography was effective for B-ELN isolation.The most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 family.Target gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system,immune system and infectious diseases.展开更多
The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal di...The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal distribution,physicochemical properties,F3′5′H domain,conserved motifs,cis-acting elements,and intron/exon compositions were analyzed.The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites.The results of qRT-PCR showed that VcF3′5′Hs(especially VcF3′5′H4)were highly expressed at the ripening stage.Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum.Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin.This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.展开更多
To improve heating uniformity and anthocyanin content of the blueberry pulp under microwave heating,the intermittent variable power microwave heating technology was introduced in the study.The effects of technology pa...To improve heating uniformity and anthocyanin content of the blueberry pulp under microwave heating,the intermittent variable power microwave heating technology was introduced in the study.The effects of technology parameters in terms of high microwave intensity heating time,intermittent time,low microwave intensity and low microwave intensity heating time on the blueberry pulp quality parameters(heating uniformity,average moisture content,the highest temperature and anthocyanin content)were investigated by using the response surface method.The results showed that the longer heating time under different microwave intensities resulted in the poorer heating uniformity.The intermittent stage promoted heat and mass transfer within the pulp and reduced the temperature difference and moisture gradient within the pulp,which enhanced desired uniformity of temperature and moisture distribution before entering the low microwave intensity heating stage.Therefore,the longer the intermittent time,the greater the heating uniformity.The optimal parameters were developed as high microwave intensity of 4 W·g^(-1),high microwave intensity heating time of 9.86 min,intermittent time of 10 min,low microwave intensity of 2.2 W·g^(-1)and low microwave intensity heating time of 6 min.This research might provide guidance for microwave heating berry fruits.展开更多
As the most common and serious disease of blueberry,blueberry canker has caused huge economic losses to the blueberry planting industry.This paper summarized the pathogenic bacteria,symptoms,regularities and preventio...As the most common and serious disease of blueberry,blueberry canker has caused huge economic losses to the blueberry planting industry.This paper summarized the pathogenic bacteria,symptoms,regularities and prevention methods of blueberry canker.展开更多
AIM:To investigate the effects of blueberry on hepatic fibrosis and NF-E2-related factor 2(Nrf2) transcription factor in rats.METHODS:Forty-five male Sprague-Dawley rats were randomly divided into control group(A);CCl...AIM:To investigate the effects of blueberry on hepatic fibrosis and NF-E2-related factor 2(Nrf2) transcription factor in rats.METHODS:Forty-five male Sprague-Dawley rats were randomly divided into control group(A);CCl4-induced hepatic fibrosis group(B);blueberry prevention group(C);Dan-shao-hua-xian capsule(DSHX) prevention group(D);and blueberry + DSHX prevention group(E).Liver fibrosis was induced in rats by subcutaneous injection of CCl4 and a high-lipid/low-protein diet for 8 wk(except the control group).The level of hyaluronic acid(HA) and alanine aminotransferase(ALT) in serum was examined.The activity of superoxide dismutase(SOD),glutathione-S-transferase(GST) and malondialdehyde(MDA) in liver homogenates was determined.The degree of hepatic fibrosis was evaluated by hematoxylin and eosin and Masson staining.Expression of Nrf2 and NADPH quinone oxidoreductase 1(Nqo1) was detected by real-time reversed transcribed-polymerase chain reaction,immunohistochemical techniques,and western blotting.RESULTS:Compared with group B,liver indices,levels of serum HA and ALT of groups C,D and E were reduced(liver indices:0.038 ± 0.008,0.036 ± 0.007,0.036 ± 0.005 vs 0.054 ± 0.009,P<0.05;HA:502.33 ± 110.57 ng/mL,524.25 ± 255.42 ng/mL,499.25 ± 198.10 ng/mL vs 828.50 ± 237.83 ng/mL,P<0.05;ALT:149.44 ± 16.51 U/L,136.88 ± 10.07 U/L,127.38 ± 11.03 U/L vs 203.25 ± 31.62 U/L,P<0.05),and SOD level was significantly higher,but MDA level was lower,in liver homogenates(SOD:1.36 ± 0.09 U/mg,1.42 ± 0.13 U/mg,1.50 ± 0.15 U/mg vs 1.08 ± 0.19 U/mg,P<0.05;MDA:0.294 ± 0.026 nmol/mg,0.285 ± 0.025 nmol/mg,0.284 ± 0.028 nmol/mg vs 0.335 ± 0.056 nmol/mg,P<0.05).Meanwhile,the stage of hepatic fibrosis was significantly weakened(P<0.05).Compared with group A,the activity of GST liver homogenates and expression levels of Nrf2 and Nqo1 in group B were elevated(P<0.05).The expression level of Nrf2 and Nqo1 in groups C,D,and E were increased as compared with group B,but the difference was not significant.CONCLUSION:Blueberry has preventive and protective effects on CCl4-induced hepatic fibrosis by reducing hepatocyte injury and lipid peroxidation.However,these effects may not be related to the activation of Nrf2 during long-term of CCl4.展开更多
BACKGROUND:Conventional drugs used in the treatment and prevention of liver diseases often have side effects,therefore research into natural substances are of significance.This study examined the effects of blueberry ...BACKGROUND:Conventional drugs used in the treatment and prevention of liver diseases often have side effects,therefore research into natural substances are of significance.This study examined the effects of blueberry on liver protection and cellular immune functions. METHODS:To determine the effects of blueberry on liver protective function,male mice were orally administered blueberry(0.6 g/10 g)or normal saline for 21 days.Hepatic RNA was extracted by Trizol reagent,and the expression of Nrf2,HO-1,and Nqo1 was determined by real-time RT- PCR.Superoxide dismutase(SOD)and malondialdehyde (MDA)in liver homogenate were determined,and liver index was measured.To assess the effects of blueberry on cellular immune function,male mice received blueberry(0.4,0.6,or 0.8 g/10 g)for 35 days,and the percentages of CD3 + ,CD4 + ,and CD8 + T lymphocyte subgroups in peripheral blood were detected by flow cytometry,the index of the thymus and spleen was measured,and lymphocyte proliferation in the spleen was determined by MTT assay. RESULTS:Blueberry treatment significantly increased the expression of Nrf2,HO-1,and Nqo1,the important antioxidant components in the liver.Hepatic SOD in the blueberry group was higher and MDA was lower than that in the control group (P【0.05).Blueberry also increased the index of the spleen and enhanced the proliferation of lymphocytes of the spleen (P【0.05).The percentages of the CD3 + and CD4 + T lymphocyte subsets and the CD4 + /CD8 + ratio were also increased by blueberry(P【0.05). CONCLUSIONS:Blueberry induces expression of Nrf2,HO-1,and Nqo1,which can protect hepatocytes from oxidative stress. In addition,blueberry can modulate T-cell function in mice.展开更多
In order to select severe overwintering abilitied blueberry cultivars for cold resistance of annual branches, promoting breeding high quality cultivars, a study had been conducted into field-planting shoots overwinter...In order to select severe overwintering abilitied blueberry cultivars for cold resistance of annual branches, promoting breeding high quality cultivars, a study had been conducted into field-planting shoots overwintering ability and the relative conductivity, MDA content, SOD of 7 different kinds of blueberries under artificial cooling process. According to survey results, under field conditions, Different cultivars of blueberries showed significantly difference, ranging from 56.67% of Bluegold to 12.80% of Darrow. With the temperature decreasing, changes of relative conductivity, MDA content and SOD in annual branches had a strong regularity and the hardiness of these cultivars is: Northland > Chippewa > Coville > Bluecroup > Darrow > Bluegold > Powderblue.展开更多
HCC(Hepatocellular Carcinoma)is a critical health issue worldwide.Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside(M3G)can regulate the progression of HCC.In this study,feces samples ...HCC(Hepatocellular Carcinoma)is a critical health issue worldwide.Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside(M3G)can regulate the progression of HCC.In this study,feces samples from the same batch of mice were collected to explore the regulatory mechanism of M3G on intestinal microbiota and microbial TCA cycle metabolism KEGG pathway in HCC mice based on 16S rRNA sequencing and metagenomics.Our results showed that blueberry M3G increased the microbial diversity and regulated the structure of intestinal microbiota in mice,such as increasing the abundance of Clostridia(butyric acid-producing bacteria),Oscillospira and Ruminococcus,and reducing the abundance of pathogenic Erysipelotrichi.Compared with the group of liver cancer and 5-fluorouracil,blueberry M3G significantly regulated microbial TCA cycle KEGG pathway via improving the expression of key proteins(porA,DLAT,aceE,PC and OGDH).Additionally,we found which the abundance of Muribaculum intestinale increased by blueberry M3G may be an important factor affecting the microbial TCA cycle KEGG pathway via the pearson correlation(R)analysis of protein and microbiota.Taken together,these results demonstrate that the blueberry M3G has the potential to be an intestinal microbiota regulator and an adjuvant to HCC therapy.展开更多
With high nutrition and health value,blueberry receives much concern in recent years. Blueberry experts in the world are cultivating new blueberry varieties. They have come up with constructive theoretical support and...With high nutrition and health value,blueberry receives much concern in recent years. Blueberry experts in the world are cultivating new blueberry varieties. They have come up with constructive theoretical support and planting technology researches and are constantly devoted to expanding blueberry planting latitudes. This paper elaborated researches of blueberry at home and abroad,current development situation of blueberry in China and Guangdong Province,analyzed strengths of planting blueberry in Guangdong. Besides,it came up with recommendations for development of blueberry industry. Finally,it discussed development prospects of blueberry planting in Guangdong.展开更多
The adaptation of blueberry to higher soil p H is poor. To explore the effect and probable mechanism of the adaptation of grafted blueberry( Vaccinium spp.) saplings to the soil environment with higher p H value. We t...The adaptation of blueberry to higher soil p H is poor. To explore the effect and probable mechanism of the adaptation of grafted blueberry( Vaccinium spp.) saplings to the soil environment with higher p H value. We took three types of saplings,( i) own-rooted Wufanshu( V. bracteatum),an economical shrub belonging to the same genus and family with blueberry,also known as oriental blueberry,( ii) own-rooted‘Sharpblue',a southern highbush blueberry( V. corymbosum) cultivar,and( iii) grafted-bluebedrry as experimental materials,which were grown in quartz sand medium for 90 d,irrigated with nutrient solution of three levels of p H values,respectively,i. e. p H 4. 6,p H 5. 8 and p H7. 0. Results showed that root development,shoot growth and dry biomass accumulation of grafted blueberry were significantly better than those of own-rooted blueberry grown in higher p H medium; compared with own-rooted blueberry saplings,leaf chlorophyll content,chlorophyll a/b ratio,chlorophyll fluorescence chemical efficiency and photosynthetic efficiency of grafted blueberry saplings grown in higher p H medium were all significantly improved. Results also showed that mineral element content of leaves,especially Mg and Fe elemental content of grafted blueberry was significantly higher than that of own-rooted blueberry saplings grown in higher p H media. Grafting-propagation of blueberry saplings taking Wufanshu as rootstock significantly enhanced the adaptation of blueberry to soil environment with higher p H value.展开更多
A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue...A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.展开更多
By using the meteorological data,GIS data and blueberry production data of Nanchong and the surrounding areas in Nanchong from 1991 to 2022,the climate ecological suitability of blueberry was analyzed based on the gro...By using the meteorological data,GIS data and blueberry production data of Nanchong and the surrounding areas in Nanchong from 1991 to 2022,the climate ecological suitability of blueberry was analyzed based on the growth habit of blueberry,and the restrictive and high-impact factors were selected as zoning indicators to carry out climate ecological zoning.The results show that Nanchong has a humid climate,and the annual average temperature in most parts is 15.0-17.8℃;the cold temperature duration in winter is more than 770 h,and the average maximum temperature in July is more than 30.0℃,so it is suitable for planting southern high-bush blueberry,rabbit-eye blueberry and a hybrid of northern and southern high-bush blueberry.Sunshine,high temperature in midsummer,daily temperature range at the fruiting stage and terrain slope are the main limiting or high-impact ecological factors of blueberry production in Nanchong,among which the adverse effect of insufficient sunshine is the largest.The meteorological disasters affecting blueberry production in Nanchong mainly include rainy weather,rainstorm and flood,high temperature and drought.High temperature and heavy heat damage in midsummer in areas at an altitude below 400 m have a great impact on the production of late-maturing blueberry.High temperature and summer drought are often accompanied in the southeast,which will aggravate the adverse effects.The suitable area of blueberry in Nanchong is mainly distributed in the shallow hilly area and the low-mountain deep hilly and gentle slope area at an altitude of 350-700 m.The less suitable area is mainly distributed in the flat dam area of shallow hill-valley at an altitude of below 350 m,the low-mountain area at an altitude of above 700 m,and other hilly areas with a slope of 15-30°.The unsuitable area is mainly distributed in hills with slope>30°,some sporadic shallow hills and flat dams at an altitude of below 300 m,river banks,water wetland buffer zones,etc.In order to make full use of the climate resources and the advantages of blueberry varieties,early-and medium-maturing southern high-bush varieties should be mainly planted in the low-altitude area below 400 m in Nanchong,and medium-and late-maturing varieties should be mainly planted in the medium-altitude area above 400 m.展开更多
Deactivation of polyphenol oxidase(PPO)in natural products is essential for downstream processing of functional molecules used as food or food additives,particularly those served as antioxidants.In the present work,we...Deactivation of polyphenol oxidase(PPO)in natural products is essential for downstream processing of functional molecules used as food or food additives,particularly those served as antioxidants.In the present work,we identified two proteins with PPO activity from lowbush blueberry using ammonium sulphate precipitation and chromatography procedures.Deactivation of these proteins was studied using aqueous solutions of ethanol of different concentrations.The PPO activity was recovered after ethanol removal for the protein samples previously soaked in a lowconcentration ethanol solution.A complete and unrecoverable deactivation of the proteins was achieved using ethanol with concentration over 70%(v/v),as manifested by the significant changes in circular dichroism(CD)and fluorescence spectroscopy measurements.Based on these findings,we propose a new extraction process for blueberry anthocyanin,in which an ethanol shock,i.e.soaking blueberry fruit in a 70%(v/v)ethanol solution for 1 h,is implemented before subsequent procedures.This new process increases the anthocyanin yield by 55%in comparison to that without the ethanol shock.展开更多
Corn starch,glycerol and agroindustrial residues were used to produce films by casting.By-products from juice processing,blueberry and jaboticaba in powder with different particle sizes were added in the filmogenic ma...Corn starch,glycerol and agroindustrial residues were used to produce films by casting.By-products from juice processing,blueberry and jaboticaba in powder with different particle sizes were added in the filmogenic matrix to evaluate its potential as a colorimetric indicator.Blueberry and jaboticaba peels are commonly discarded although contain high amount of important compounds as anthocyanins.These compounds have the ability to color change after immersion in different pH values,demonstrating its potential for the intelligent packaging development.Analyses were performed in a colorimeter after films immersion in different buffer solutions.Visual color changes were perceived;reddish and bluish color in acidic and basic pH values,respectively.Independently of the particle size,theΔE* values were greater than 5,showing visually perceptible change to the human eye.The results indicate the potential of use of these residues as a pH indicator for the development of renewable and biodegradable sensor of food deterioration.展开更多
Blueberry is a kind of wild berry with high nutrition values. It contains a great amount of anthocyanin, organic acid and fiber, which significantly affects sensory evaluation, pH value, texture profile analysis (TPA)...Blueberry is a kind of wild berry with high nutrition values. It contains a great amount of anthocyanin, organic acid and fiber, which significantly affects sensory evaluation, pH value, texture profile analysis (TPA) and microstructure. This study determined the effect of blueberry pulp on spreadable processed cheese through examining pH value, TPA, microstructure and color-difference. The results showed that increased blueberry content lowered pH value, remarkably transformed microstructure and TPA (P<0.05). Overloaded fiber in blueberry ruined the network structure of casein gel, thus 10% of blueberry performed better, more than that additive volume, sensory evaluation was worsen and gel structure was spoiled gradually. The blueberry sample lowered L*, b* and slightly increased a*, resulting in blueberry samples presented light amethyst, while sterilizing samples presented dark pink because of the lost of anthocyanin.展开更多
In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave p...In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave power, initial moisture content, puffing time and mass were employed as experimental factors. The pressure, temperature, amount of water vapor and puffing properties in terms of the volume, hardness and brittleness were employed as target parameters. The higher microwave intensity and initial moisture content resulted in the more amount of water vapor in the snacks and more holes in the appropriate puffing time. The shape and structure of the snacks tended to be stable with the removal of moisture.展开更多
The root system plays an important role in the growth and development of blueberry.The aim of this study was to assess the impacts of different fertilizers on the root growth and root–yield relationship of blueberry ...The root system plays an important role in the growth and development of blueberry.The aim of this study was to assess the impacts of different fertilizers on the root growth and root–yield relationship of blueberry to provide insight into the regulation of root growth and fruit yield by fertilizing from the perspective of the root system.Rabbiteye blueberry variety‘Britewell’as the test material,and six fertilizers,including BF,OR,CF,SF,HF,and RT were used in single-factor fertilization experiments to analyze the effects of different fertilizer treatments on the root morphology,root distribution,and fruit yield of blueberry.Fertilization overall increased the root length density and root surface area in most soil layers,and the RT treatment significantly increased the total root length density and total root surface area 98.6%and 98.5%,respectively,compared with a control lacking fertilizer.In addition,the effect of fertilization on the blueberry root system was mainly observed in the 0–20 cm layer.Fruit yield was positively correlated with total root length density and total root surface area,and negatively correlated with average root diameter.In summary,the SF and RT treatments increased the morphological indexes of the root system,particularly in the shallow soil layers,leading to an increase in blueberry fruit yield.展开更多
The protective effects of blueberry anthocyanin extracts against damage induced by extremely lowfrequency electromagnetic field(ELF-EMF)were investigated in a rat model.Wistar rats were exposed to ELF-EMF with or with...The protective effects of blueberry anthocyanin extracts against damage induced by extremely lowfrequency electromagnetic field(ELF-EMF)were investigated in a rat model.Wistar rats were exposed to ELF-EMF with or without the administration of blueberry anthocyanin extracts(50,100,and 200 mg/kg per day intragastrically once a day)for 30 days.Blueberry anthocyanin extracts supplementation inhibited the decrease in Nissl substance levels,cell membrane integrity,and mitochondrial membrane potential induced by ELF-EMF;prevented the increase in nitric oxide,malondialdehyde,and Ca2+concentrations;suppressed superoxide dismutase and glutathione depletion;and enhanced the cognitive ability of the rats exposed to ELF-EMF.The protective effects of blueberry anthocyanin extracts against hippocampal neuron injury caused by ELF-EMF were dose-dependent.These results demonstrated that blueberry anthocyanin extracts suppress hippocampal neuron injury caused by ELF-EMF by inhibiting cell membrane damage and oxidative stress pathways,and suggested that blueberry anthocyanin treatment potentially prevents hippocampal neuron injury.展开更多
[Objective] The paper was to clear the control efficiency of drug painting on different pruning wounds and different pruning lengths against secondary infection of blueberry twig blight. [Method] With self-made cream,...[Objective] The paper was to clear the control efficiency of drug painting on different pruning wounds and different pruning lengths against secondary infection of blueberry twig blight. [Method] With self-made cream,heal wound and 10 times 45% lime sulfur as materials,the control effects against blueberry twig blight by different lengths of pruning and painting different creams were studied. [Result] The probability of infection was lower when the pruning wounds were farther way from the diseased branches. Pruning branches at 15 cm away from infected symptoms and painting wounds with self-made cream received the best effort. The control efficacy of painting self-made cream was 100% in the first year,and 85. 71% in the following year. The control efficacy of painting heal wound was 55% in the first year,and 22. 63% in the following year. The control efficacy of painting lime sulfur was 42. 07% in the first year,and 30. 56% in the following year. [Conclusion] Pruning branches at 15 cm away from infected symptoms and painting wounds with self-made cream could reduce the secondary infection of newborn branches.展开更多
About 60% of blueberry (Vaccinium corymbosum) plantations in Argentina are located in the provinces of Entre Ríos and Buenos Aires. Alternaria tenuissima and Pestalotiopsis guepinii have been reported as pathogen...About 60% of blueberry (Vaccinium corymbosum) plantations in Argentina are located in the provinces of Entre Ríos and Buenos Aires. Alternaria tenuissima and Pestalotiopsis guepinii have been reported as pathogens of blueberry, causing leaf spots and branch cankers. The aims of this research were to estimate the prevalence of these microorganisms on leaves and fruits taken from crops located in Entre Ríos and Buenos Aires, as well as to differentiate leaf symptoms after target inoculations with each pathogen individually and in mixtures. Both fungi were present in blueberry fields from 2010 to 2013. A. tenuissima was the most prevalent pathogen, as most of the symptoms detected in the fields had been caused by this species. As a result of inoculations on cv. O’Neal, injured tissues showed symptoms before undamaged ones. Leaf symptoms caused by A. tenuissima differed from those caused by P. guepinii because of their predominant reddish color and the absence of drop-off of the central part of the lesions. When inoculated in a mixture, incubation period on leaves was intermediate between the registered for individual inoculations. The leaves showed reddishbrown spots typical of A. tenuissima and dark brown spots typical of P. guepinii, both with red margins. Blight, defoliation and canker symptoms caused in each case were undistinguishable.展开更多
基金supported by the National Natural Science Foundation of China(31701561)。
文摘Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical functions.However,the diversity of plant materials makes the plant derived ELN study challenging.More efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human health.In this study,a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs(B-ELNs),and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing.A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue,and their potential cross-kingdom human gene targets were further predicted.Results showed that size exclusion chromatography was effective for B-ELN isolation.The most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 family.Target gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system,immune system and infectious diseases.
基金funded by the National Natural Science Foundation of China(Grant No.31760205)Guangdong Basic and Applied Basic Research Foundation of Guangdong Province-Yuehui Joint Foundation(Grant No.2022A1515111095)+1 种基金the Professor and Doctoral Research Project(Grant No.2022JB021)in Huizhou Universitythe Project of the Department of Education of Guangdong Province(Grant No.2018KTSCX214).
文摘The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal distribution,physicochemical properties,F3′5′H domain,conserved motifs,cis-acting elements,and intron/exon compositions were analyzed.The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites.The results of qRT-PCR showed that VcF3′5′Hs(especially VcF3′5′H4)were highly expressed at the ripening stage.Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum.Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin.This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.
基金Supported by the National Natural Science Foundation of China(32072352)。
文摘To improve heating uniformity and anthocyanin content of the blueberry pulp under microwave heating,the intermittent variable power microwave heating technology was introduced in the study.The effects of technology parameters in terms of high microwave intensity heating time,intermittent time,low microwave intensity and low microwave intensity heating time on the blueberry pulp quality parameters(heating uniformity,average moisture content,the highest temperature and anthocyanin content)were investigated by using the response surface method.The results showed that the longer heating time under different microwave intensities resulted in the poorer heating uniformity.The intermittent stage promoted heat and mass transfer within the pulp and reduced the temperature difference and moisture gradient within the pulp,which enhanced desired uniformity of temperature and moisture distribution before entering the low microwave intensity heating stage.Therefore,the longer the intermittent time,the greater the heating uniformity.The optimal parameters were developed as high microwave intensity of 4 W·g^(-1),high microwave intensity heating time of 9.86 min,intermittent time of 10 min,low microwave intensity of 2.2 W·g^(-1)and low microwave intensity heating time of 6 min.This research might provide guidance for microwave heating berry fruits.
基金Key Technical Research Project of Jilin Provincial Department of Science and Technology(20190301043NY)Jilin Provincial Innovation and Entrepreneurship Talent Funding Project(2020036)Forestry Science and Technology Innovation Platform Operation Subsidy Project(2020132032)。
文摘As the most common and serious disease of blueberry,blueberry canker has caused huge economic losses to the blueberry planting industry.This paper summarized the pathogenic bacteria,symptoms,regularities and prevention methods of blueberry canker.
基金Supported by A grant from Foundation of High Level Talented Specialists of Guizhou Province,China,No. TZJF-200850a grant from Foundation of the Program for Tackling Key Problems of Guizhou Science and Technology Department,China,No. 2010GZ97666
文摘AIM:To investigate the effects of blueberry on hepatic fibrosis and NF-E2-related factor 2(Nrf2) transcription factor in rats.METHODS:Forty-five male Sprague-Dawley rats were randomly divided into control group(A);CCl4-induced hepatic fibrosis group(B);blueberry prevention group(C);Dan-shao-hua-xian capsule(DSHX) prevention group(D);and blueberry + DSHX prevention group(E).Liver fibrosis was induced in rats by subcutaneous injection of CCl4 and a high-lipid/low-protein diet for 8 wk(except the control group).The level of hyaluronic acid(HA) and alanine aminotransferase(ALT) in serum was examined.The activity of superoxide dismutase(SOD),glutathione-S-transferase(GST) and malondialdehyde(MDA) in liver homogenates was determined.The degree of hepatic fibrosis was evaluated by hematoxylin and eosin and Masson staining.Expression of Nrf2 and NADPH quinone oxidoreductase 1(Nqo1) was detected by real-time reversed transcribed-polymerase chain reaction,immunohistochemical techniques,and western blotting.RESULTS:Compared with group B,liver indices,levels of serum HA and ALT of groups C,D and E were reduced(liver indices:0.038 ± 0.008,0.036 ± 0.007,0.036 ± 0.005 vs 0.054 ± 0.009,P<0.05;HA:502.33 ± 110.57 ng/mL,524.25 ± 255.42 ng/mL,499.25 ± 198.10 ng/mL vs 828.50 ± 237.83 ng/mL,P<0.05;ALT:149.44 ± 16.51 U/L,136.88 ± 10.07 U/L,127.38 ± 11.03 U/L vs 203.25 ± 31.62 U/L,P<0.05),and SOD level was significantly higher,but MDA level was lower,in liver homogenates(SOD:1.36 ± 0.09 U/mg,1.42 ± 0.13 U/mg,1.50 ± 0.15 U/mg vs 1.08 ± 0.19 U/mg,P<0.05;MDA:0.294 ± 0.026 nmol/mg,0.285 ± 0.025 nmol/mg,0.284 ± 0.028 nmol/mg vs 0.335 ± 0.056 nmol/mg,P<0.05).Meanwhile,the stage of hepatic fibrosis was significantly weakened(P<0.05).Compared with group A,the activity of GST liver homogenates and expression levels of Nrf2 and Nqo1 in group B were elevated(P<0.05).The expression level of Nrf2 and Nqo1 in groups C,D,and E were increased as compared with group B,but the difference was not significant.CONCLUSION:Blueberry has preventive and protective effects on CCl4-induced hepatic fibrosis by reducing hepatocyte injury and lipid peroxidation.However,these effects may not be related to the activation of Nrf2 during long-term of CCl4.
文摘BACKGROUND:Conventional drugs used in the treatment and prevention of liver diseases often have side effects,therefore research into natural substances are of significance.This study examined the effects of blueberry on liver protection and cellular immune functions. METHODS:To determine the effects of blueberry on liver protective function,male mice were orally administered blueberry(0.6 g/10 g)or normal saline for 21 days.Hepatic RNA was extracted by Trizol reagent,and the expression of Nrf2,HO-1,and Nqo1 was determined by real-time RT- PCR.Superoxide dismutase(SOD)and malondialdehyde (MDA)in liver homogenate were determined,and liver index was measured.To assess the effects of blueberry on cellular immune function,male mice received blueberry(0.4,0.6,or 0.8 g/10 g)for 35 days,and the percentages of CD3 + ,CD4 + ,and CD8 + T lymphocyte subgroups in peripheral blood were detected by flow cytometry,the index of the thymus and spleen was measured,and lymphocyte proliferation in the spleen was determined by MTT assay. RESULTS:Blueberry treatment significantly increased the expression of Nrf2,HO-1,and Nqo1,the important antioxidant components in the liver.Hepatic SOD in the blueberry group was higher and MDA was lower than that in the control group (P【0.05).Blueberry also increased the index of the spleen and enhanced the proliferation of lymphocytes of the spleen (P【0.05).The percentages of the CD3 + and CD4 + T lymphocyte subsets and the CD4 + /CD8 + ratio were also increased by blueberry(P【0.05). CONCLUSIONS:Blueberry induces expression of Nrf2,HO-1,and Nqo1,which can protect hepatocytes from oxidative stress. In addition,blueberry can modulate T-cell function in mice.
文摘In order to select severe overwintering abilitied blueberry cultivars for cold resistance of annual branches, promoting breeding high quality cultivars, a study had been conducted into field-planting shoots overwintering ability and the relative conductivity, MDA content, SOD of 7 different kinds of blueberries under artificial cooling process. According to survey results, under field conditions, Different cultivars of blueberries showed significantly difference, ranging from 56.67% of Bluegold to 12.80% of Darrow. With the temperature decreasing, changes of relative conductivity, MDA content and SOD in annual branches had a strong regularity and the hardiness of these cultivars is: Northland > Chippewa > Coville > Bluecroup > Darrow > Bluegold > Powderblue.
基金supported by the National Natural Science Foundation of China(31972090)LiaoNing Revitalization Talents Program(XLYC1807127)+3 种基金Liaoning BaiQianWan Talents Program(2018-B-21)Tianzhu Mountain Scholar Support Project(2018)the Innovative Talent Support Program for Institution of Higher Learning of Liaoning Province(LR2017038)the project of“double hundred”for major scientific and technological achievements transformating of Shenyang Science and Technology Bureau(Z19-3-012).
文摘HCC(Hepatocellular Carcinoma)is a critical health issue worldwide.Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside(M3G)can regulate the progression of HCC.In this study,feces samples from the same batch of mice were collected to explore the regulatory mechanism of M3G on intestinal microbiota and microbial TCA cycle metabolism KEGG pathway in HCC mice based on 16S rRNA sequencing and metagenomics.Our results showed that blueberry M3G increased the microbial diversity and regulated the structure of intestinal microbiota in mice,such as increasing the abundance of Clostridia(butyric acid-producing bacteria),Oscillospira and Ruminococcus,and reducing the abundance of pathogenic Erysipelotrichi.Compared with the group of liver cancer and 5-fluorouracil,blueberry M3G significantly regulated microbial TCA cycle KEGG pathway via improving the expression of key proteins(porA,DLAT,aceE,PC and OGDH).Additionally,we found which the abundance of Muribaculum intestinale increased by blueberry M3G may be an important factor affecting the microbial TCA cycle KEGG pathway via the pearson correlation(R)analysis of protein and microbiota.Taken together,these results demonstrate that the blueberry M3G has the potential to be an intestinal microbiota regulator and an adjuvant to HCC therapy.
基金Supported by National Science and Technology Project of the Ministry of Science and Technology in the Twelfth Five-Year Plan Period(2012BAD22B0504)Special Project on the Integration of Industry,Education and Research of Guangdong Province(2013B090200065)Science and Technology Planning Project of Guangdong Province(2012B050500009)
文摘With high nutrition and health value,blueberry receives much concern in recent years. Blueberry experts in the world are cultivating new blueberry varieties. They have come up with constructive theoretical support and planting technology researches and are constantly devoted to expanding blueberry planting latitudes. This paper elaborated researches of blueberry at home and abroad,current development situation of blueberry in China and Guangdong Province,analyzed strengths of planting blueberry in Guangdong. Besides,it came up with recommendations for development of blueberry industry. Finally,it discussed development prospects of blueberry planting in Guangdong.
基金Supported by National Natural Science Foundation(31270674)Important Innovation Research Project of Zhaoqing University(CQ201607)Science and Technology Project of Liyang Tianmu Lake Longtan Eco-Technology Valley Co.,Ltd
文摘The adaptation of blueberry to higher soil p H is poor. To explore the effect and probable mechanism of the adaptation of grafted blueberry( Vaccinium spp.) saplings to the soil environment with higher p H value. We took three types of saplings,( i) own-rooted Wufanshu( V. bracteatum),an economical shrub belonging to the same genus and family with blueberry,also known as oriental blueberry,( ii) own-rooted‘Sharpblue',a southern highbush blueberry( V. corymbosum) cultivar,and( iii) grafted-bluebedrry as experimental materials,which were grown in quartz sand medium for 90 d,irrigated with nutrient solution of three levels of p H values,respectively,i. e. p H 4. 6,p H 5. 8 and p H7. 0. Results showed that root development,shoot growth and dry biomass accumulation of grafted blueberry were significantly better than those of own-rooted blueberry grown in higher p H medium; compared with own-rooted blueberry saplings,leaf chlorophyll content,chlorophyll a/b ratio,chlorophyll fluorescence chemical efficiency and photosynthetic efficiency of grafted blueberry saplings grown in higher p H medium were all significantly improved. Results also showed that mineral element content of leaves,especially Mg and Fe elemental content of grafted blueberry was significantly higher than that of own-rooted blueberry saplings grown in higher p H media. Grafting-propagation of blueberry saplings taking Wufanshu as rootstock significantly enhanced the adaptation of blueberry to soil environment with higher p H value.
文摘A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.
基金Supported by the Foundation of Scientific and Technological Development of Meteorological Administration/Heavy Rain and Drought-Flood Disasters in Plateau and Basin Key Laboratory of Sichuan Province(Key Laboratory of Sichuan Province-2018-Key-05-03)the Special Project for Applied Technology Research and Development in Nanchong City(21YFZJ0027)。
文摘By using the meteorological data,GIS data and blueberry production data of Nanchong and the surrounding areas in Nanchong from 1991 to 2022,the climate ecological suitability of blueberry was analyzed based on the growth habit of blueberry,and the restrictive and high-impact factors were selected as zoning indicators to carry out climate ecological zoning.The results show that Nanchong has a humid climate,and the annual average temperature in most parts is 15.0-17.8℃;the cold temperature duration in winter is more than 770 h,and the average maximum temperature in July is more than 30.0℃,so it is suitable for planting southern high-bush blueberry,rabbit-eye blueberry and a hybrid of northern and southern high-bush blueberry.Sunshine,high temperature in midsummer,daily temperature range at the fruiting stage and terrain slope are the main limiting or high-impact ecological factors of blueberry production in Nanchong,among which the adverse effect of insufficient sunshine is the largest.The meteorological disasters affecting blueberry production in Nanchong mainly include rainy weather,rainstorm and flood,high temperature and drought.High temperature and heavy heat damage in midsummer in areas at an altitude below 400 m have a great impact on the production of late-maturing blueberry.High temperature and summer drought are often accompanied in the southeast,which will aggravate the adverse effects.The suitable area of blueberry in Nanchong is mainly distributed in the shallow hilly area and the low-mountain deep hilly and gentle slope area at an altitude of 350-700 m.The less suitable area is mainly distributed in the flat dam area of shallow hill-valley at an altitude of below 350 m,the low-mountain area at an altitude of above 700 m,and other hilly areas with a slope of 15-30°.The unsuitable area is mainly distributed in hills with slope>30°,some sporadic shallow hills and flat dams at an altitude of below 300 m,river banks,water wetland buffer zones,etc.In order to make full use of the climate resources and the advantages of blueberry varieties,early-and medium-maturing southern high-bush varieties should be mainly planted in the low-altitude area below 400 m in Nanchong,and medium-and late-maturing varieties should be mainly planted in the medium-altitude area above 400 m.
基金supported by Xinjiang Tianjianhemu Biotech Co.Ltd under project contract No.20172000941.
文摘Deactivation of polyphenol oxidase(PPO)in natural products is essential for downstream processing of functional molecules used as food or food additives,particularly those served as antioxidants.In the present work,we identified two proteins with PPO activity from lowbush blueberry using ammonium sulphate precipitation and chromatography procedures.Deactivation of these proteins was studied using aqueous solutions of ethanol of different concentrations.The PPO activity was recovered after ethanol removal for the protein samples previously soaked in a lowconcentration ethanol solution.A complete and unrecoverable deactivation of the proteins was achieved using ethanol with concentration over 70%(v/v),as manifested by the significant changes in circular dichroism(CD)and fluorescence spectroscopy measurements.Based on these findings,we propose a new extraction process for blueberry anthocyanin,in which an ethanol shock,i.e.soaking blueberry fruit in a 70%(v/v)ethanol solution for 1 h,is implemented before subsequent procedures.This new process increases the anthocyanin yield by 55%in comparison to that without the ethanol shock.
文摘Corn starch,glycerol and agroindustrial residues were used to produce films by casting.By-products from juice processing,blueberry and jaboticaba in powder with different particle sizes were added in the filmogenic matrix to evaluate its potential as a colorimetric indicator.Blueberry and jaboticaba peels are commonly discarded although contain high amount of important compounds as anthocyanins.These compounds have the ability to color change after immersion in different pH values,demonstrating its potential for the intelligent packaging development.Analyses were performed in a colorimeter after films immersion in different buffer solutions.Visual color changes were perceived;reddish and bluish color in acidic and basic pH values,respectively.Independently of the particle size,theΔE* values were greater than 5,showing visually perceptible change to the human eye.The results indicate the potential of use of these residues as a pH indicator for the development of renewable and biodegradable sensor of food deterioration.
基金Supported by Scientific Research Fund of Northeast Agricultural University
文摘Blueberry is a kind of wild berry with high nutrition values. It contains a great amount of anthocyanin, organic acid and fiber, which significantly affects sensory evaluation, pH value, texture profile analysis (TPA) and microstructure. This study determined the effect of blueberry pulp on spreadable processed cheese through examining pH value, TPA, microstructure and color-difference. The results showed that increased blueberry content lowered pH value, remarkably transformed microstructure and TPA (P<0.05). Overloaded fiber in blueberry ruined the network structure of casein gel, thus 10% of blueberry performed better, more than that additive volume, sensory evaluation was worsen and gel structure was spoiled gradually. The blueberry sample lowered L*, b* and slightly increased a*, resulting in blueberry samples presented light amethyst, while sterilizing samples presented dark pink because of the lost of anthocyanin.
基金Supported by National Natural Science Foundation of China(31571848)
文摘In order to reveal the mechanism of microwave puffing berry snack, a center combination experiment method was employed to explore the puffing process of blueberry snacks. Blueberry as the raw material, the microwave power, initial moisture content, puffing time and mass were employed as experimental factors. The pressure, temperature, amount of water vapor and puffing properties in terms of the volume, hardness and brittleness were employed as target parameters. The higher microwave intensity and initial moisture content resulted in the more amount of water vapor in the snacks and more holes in the appropriate puffing time. The shape and structure of the snacks tended to be stable with the removal of moisture.
基金the National Natural Science Foundation of China,Grant No.“31760205”.
文摘The root system plays an important role in the growth and development of blueberry.The aim of this study was to assess the impacts of different fertilizers on the root growth and root–yield relationship of blueberry to provide insight into the regulation of root growth and fruit yield by fertilizing from the perspective of the root system.Rabbiteye blueberry variety‘Britewell’as the test material,and six fertilizers,including BF,OR,CF,SF,HF,and RT were used in single-factor fertilization experiments to analyze the effects of different fertilizer treatments on the root morphology,root distribution,and fruit yield of blueberry.Fertilization overall increased the root length density and root surface area in most soil layers,and the RT treatment significantly increased the total root length density and total root surface area 98.6%and 98.5%,respectively,compared with a control lacking fertilizer.In addition,the effect of fertilization on the blueberry root system was mainly observed in the 0–20 cm layer.Fruit yield was positively correlated with total root length density and total root surface area,and negatively correlated with average root diameter.In summary,the SF and RT treatments increased the morphological indexes of the root system,particularly in the shallow soil layers,leading to an increase in blueberry fruit yield.
基金supported by the Natural Science Foundation Key Program of Liaoning Province(20170540803)the Liaoning Provincial Department of Education Project(LSNJC201911).
文摘The protective effects of blueberry anthocyanin extracts against damage induced by extremely lowfrequency electromagnetic field(ELF-EMF)were investigated in a rat model.Wistar rats were exposed to ELF-EMF with or without the administration of blueberry anthocyanin extracts(50,100,and 200 mg/kg per day intragastrically once a day)for 30 days.Blueberry anthocyanin extracts supplementation inhibited the decrease in Nissl substance levels,cell membrane integrity,and mitochondrial membrane potential induced by ELF-EMF;prevented the increase in nitric oxide,malondialdehyde,and Ca2+concentrations;suppressed superoxide dismutase and glutathione depletion;and enhanced the cognitive ability of the rats exposed to ELF-EMF.The protective effects of blueberry anthocyanin extracts against hippocampal neuron injury caused by ELF-EMF were dose-dependent.These results demonstrated that blueberry anthocyanin extracts suppress hippocampal neuron injury caused by ELF-EMF by inhibiting cell membrane damage and oxidative stress pathways,and suggested that blueberry anthocyanin treatment potentially prevents hippocampal neuron injury.
基金Supported by Special Fund for Scientific Research (Forestry) in the Public Interest(201204402)National Spark Program(2014GA741014)
文摘[Objective] The paper was to clear the control efficiency of drug painting on different pruning wounds and different pruning lengths against secondary infection of blueberry twig blight. [Method] With self-made cream,heal wound and 10 times 45% lime sulfur as materials,the control effects against blueberry twig blight by different lengths of pruning and painting different creams were studied. [Result] The probability of infection was lower when the pruning wounds were farther way from the diseased branches. Pruning branches at 15 cm away from infected symptoms and painting wounds with self-made cream received the best effort. The control efficacy of painting self-made cream was 100% in the first year,and 85. 71% in the following year. The control efficacy of painting heal wound was 55% in the first year,and 22. 63% in the following year. The control efficacy of painting lime sulfur was 42. 07% in the first year,and 30. 56% in the following year. [Conclusion] Pruning branches at 15 cm away from infected symptoms and painting wounds with self-made cream could reduce the secondary infection of newborn branches.
文摘About 60% of blueberry (Vaccinium corymbosum) plantations in Argentina are located in the provinces of Entre Ríos and Buenos Aires. Alternaria tenuissima and Pestalotiopsis guepinii have been reported as pathogens of blueberry, causing leaf spots and branch cankers. The aims of this research were to estimate the prevalence of these microorganisms on leaves and fruits taken from crops located in Entre Ríos and Buenos Aires, as well as to differentiate leaf symptoms after target inoculations with each pathogen individually and in mixtures. Both fungi were present in blueberry fields from 2010 to 2013. A. tenuissima was the most prevalent pathogen, as most of the symptoms detected in the fields had been caused by this species. As a result of inoculations on cv. O’Neal, injured tissues showed symptoms before undamaged ones. Leaf symptoms caused by A. tenuissima differed from those caused by P. guepinii because of their predominant reddish color and the absence of drop-off of the central part of the lesions. When inoculated in a mixture, incubation period on leaves was intermediate between the registered for individual inoculations. The leaves showed reddishbrown spots typical of A. tenuissima and dark brown spots typical of P. guepinii, both with red margins. Blight, defoliation and canker symptoms caused in each case were undistinguishable.
