Plant of Bambusa vulgaris is used in traditional medicine practice for the management of many pathologies including hypertension. The effect of the aqueous extract of the leaves of Bambusa vulgaris on blood pressure w...Plant of Bambusa vulgaris is used in traditional medicine practice for the management of many pathologies including hypertension. The effect of the aqueous extract of the leaves of Bambusa vulgaris on blood pressure was evaluated in normotensive male rabbits. Blood pressure was measured by the invasive method. Thus, aqueous extract of Bambusa vulgaris leaves had been injected intravenously at doses ranging from 1 mg/kg to 30 mg/kg in rabbits anesthetized with thiopental. The effects of this extract on blood pressure were also evaluated in rabbits having previously received different doses of atropine. The aqueous extract of Bambusa vulgaris leaves induced a dose-dependent hypotension which is not canceled in the presence of atropine. The results obtained show that the extract has blood pressure lowering effect which may be mediated by muscarinic-type cholinergic receptors.展开更多
Plants have evolved constitutive and inductive defense mechanisms to protect themselves from herbivorous insects.Metabolites in plants are thought to be involved in response to herbivores.Bambusa emeiensis is an impor...Plants have evolved constitutive and inductive defense mechanisms to protect themselves from herbivorous insects.Metabolites in plants are thought to be involved in response to herbivores.Bambusa emeiensis is an important bamboo species widely distributed in Southwest China.It is easy to attract herbivores such as Cyrtotrachelus buqueti.Through the detection and analysis of metabolites in B.emeiensis metabolomic data,35 differential metabolites(biomarkers)were finally identified from 206 detection peaks,mainly primary metabolites.Subsequently,we conducted an association analysis between 35 biomarkers that annotated to be involved in 71 metabolic pathways in the metabolome and 324 differentially expressed genes(DEGs)in 207 gene expression pathways distributed in the transcriptome of B.emeiensis after feeding by C.buqueti.We also discussed the relationship between the changes in gene expression levels and metabolite content variation.A total of 47 integrated pathways containing the corresponding DEGs and biomarkers were screened out,with the amino acid synthesis pathway(ko01230)containing the most DEGs and biomarkers.In these integrated pathways,the changes in biomarkers content and the expression levels of the corresponding genes were generally consistent.For example,the increase in tryptophan content was accompanied by an increase in the expression of the corresponding catalase in the tryptophan synthesis pathway.Similar to this was glucose and trehalose in carbohydrate metabolism.Therefore,this study further deepened our understanding of the defense mechanism of B.emeiensis against bamboo pests and provided new insights for the prevention and control of bamboo diseases and pests.展开更多
Dwindling supplies of bamboo resources and the increase in the demand for bamboo ha</span></span><span><span><span style="font-family:"">ve</span></span></sp...Dwindling supplies of bamboo resources and the increase in the demand for bamboo ha</span></span><span><span><span style="font-family:"">ve</span></span></span><span><span><span style="font-family:""> led to the need for better propagation techniques. This is the only way to ensure the sustainability of the bamboo resource base and improve livelihoods. Thus, this study was carried out to examine the different growth parameters of culm cuttings of <i>Bambusa vulgaris</i> in propagation beds with a substrate consisting of dark soil, manure (compost) and sand in a ratio of 1:1:1. Data on different growth parameters was collected on a daily basis over a three week period and analyzed using appropriate descriptive and inferential statistics. It was found that the different growth parameters (diameter, height, leaves and sheaths) of culm cuttings of <i>B. vulgaris</i> evolved in like manner in three propagation beds. Descriptive statistics for growth parameters of culm cuttings of <i>B. vulgaris</i> showed little or no differences across three shelter/propagation beds (shelter bed 1, shelter bed 2 and shelter bed 3) with 1.4675 ± 1.6172 cm, 93 ± 112 cm, 22 ± 30 leaves, and 4 ± 6 sheaths being the mean diameter, height, number of leaves and number of sheaths for all three propagation beds, respectively. ANOVA test showed no statistically significant variation (<i>p</i> > 0.05) between growth parameters of culm cuttings of <i>B. vulgaris</i> across the three propagation beds, implying that the different growth parameters were relatively similar. Pearson correlation showed the existence of direct and inverse relationships between the different growth parameters of culm cuttings of bamboo. The existence of these direct and inverse relationships could be attributed to the rapid growth rate of bamboo, the substrate used, and the species of bamboo used (<i>B. vulgaris</i>). The study recommends more large-scale propagation projects of this bamboo species in order to ensure the sustainability of the bamboo resource base and improve livelihoods of bamboo dependent rural communities.展开更多
Bambusa vulgaris Schrad ex wendl is a widely cultivated bamboo species in rural Bangladesh for its versatile uses. The vegetative propagation becomes the only viable alternative for this species because B. vulgaris do...Bambusa vulgaris Schrad ex wendl is a widely cultivated bamboo species in rural Bangladesh for its versatile uses. The vegetative propagation becomes the only viable alternative for this species because B. vulgaris does not set seed after sparse flowering, which makes seedling progenies unavailable. A low-cost propagation trial was conducted to explore the clonal propagation techniques for the species with two types of small branch cuttings, nodal leafy cuttings and tip cuttings. The cuttings, after treating with 0, 0.1%, 0.4%, and 0.8% IBA solutions, were kept in non-mist propagator to let them to root for assessing the rooting ability. The cuttings were rooted in four weeks and were allowed to grow in the polybags for 10 months under nursery condition to assess their steckling capacity. The study reveals that both types of branch cuttings are able to develop roots, shoots, to survive and to form rhizome under the nursery condition. Rooting ability of the cuttings was significantly enhanced by the application of rooting hormone - IBA. The highest rooting percentage in nodal leafy cuttings and the tip cuttings (56.67% and 51.0%, respectively) were observed in 0.8% IBA treatment, followed by 0.4% IBA and the lowest (34.3% and 30.0%, respectively) was in control. The highest number of root developed per cutting (9.77 and 8.33 in nodal leafy cuttings and the tip cuttings, respectively) was also obtained from the cuttings treated with 0.8% IBA solution, followed 0.4% IBA treatment and the lowest (3.1 and 2.1, respectively) was in the cuttings without treatment. However, the length of the longest root varied significantly neither with the cutting types nor the concentrations of IBA solution. Survival percentage of the stecklings in nursery condition was significantly enhanced by IBA.展开更多
Fermentation of the succulent bamboo shoots of Bambusa balcooa Roxb. resulted in an enrichment of phytosterols from 0.12% to 0.62% dry weight as compared to that of the fresh unfermented samples. The bacterial strains...Fermentation of the succulent bamboo shoots of Bambusa balcooa Roxb. resulted in an enrichment of phytosterols from 0.12% to 0.62% dry weight as compared to that of the fresh unfermented samples. The bacterial strains responsible for higher accumulation of phytosterols during fermentation of the bamboo shoots have been isolated and further extraction and purification of the crude phytosterols (isolated from the fermented samples) were done by TLC, UV, NMR, IR and Mass spectral analysis. The isolated phytosterols (β-sitos-terols) were then subjected to microbial transformation which yielded a considerable amount of androsta-1, 4-diene-3,17-dione (ADD) in the incubation mixture in presence of metabolic inhibitors (α, α＇-dipyridyl and sodium arsenate).展开更多
Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species.In the present inv...Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species.In the present investigation the nodal segment explants of Bambusa tulda Roxb collected in different seasons and treated with various concentrations of HgCl_2 showed significant variation in aseptic culture establishment and bud break.The rainy season(July–August) recorded with highest of 78% aseptic culture establishment whereas autumn recorded with lowest 46%.Summer and winter seasons emerged to be the best period, registering>60% in vitro bud break.On the other hand, the autumn season had the lowest value for bud break,i.e.42%.Among different doses of sterilizing agent tried,HgCl_2 0.1% found to be suitable for maximum aseptic culture establishment(66%) as well as bud break(59%).However, among the interactions, summer season and the dose of 0.1% HgCl_2 exhibited maximum of 73% response for both aseptic culture establishment and bud break.MS medium(liquid) enriched with 5.0 μM BA+5.0 μM Kn[Kinetin(N6-Furfuryladenine)] with additional supplementation of 100 μM glutamine+0.1 μM IAA supported a maximum in vitro shoot multiplication of 4.75 fold.The proliferated shoots were successfully rooted on MS medium(liquid) supplemented 40 μM coumarin.The plantlets transferred to the polythene bags showed 98% survival.展开更多
[Objective] The purpose was to provide a reference for crossbreeding of grass carp. [Method]We conducted hybridization between Ctenopharyngodon idellus( ♀) and Elopichthys bambusa(♂) by artificial methods. The proce...[Objective] The purpose was to provide a reference for crossbreeding of grass carp. [Method]We conducted hybridization between Ctenopharyngodon idellus( ♀) and Elopichthys bambusa(♂) by artificial methods. The process of embryo and postembryonic development were observed and recorded. [Result]The fertilization rate,hatching rate and survival rate of the hybrid F1were( 75. 8 ± 6. 2) %,( 41. 9 ± 8. 2) % and( 9. 3 ± 3. 7) %,respectively. At the water temperature of 20. 1- 21. 6℃,the larvae was hatched for about 34 h and 25 min after fertilization. The whole length of newly-hatched larvae of the hybrid F1was( 5. 8 ± 0. 12) mm. The larvae could feed rotifer and unicellular algae after 3 or 4 days hatching. Postembryonic development of the hybrid at the formation of scales,lasted for 65 days when the young was( 74. 0 ± 2. 1) mm in whole length. [Conclusion]The embryonic development of hybrid F1 was intermediate to their parents,the form of the hybrid F1 was similar to that of their female line of grass carp. The growth rate of larva was faster than that of grass carp and similar to that of their paternal fish.展开更多
The influence of seasonal variation, indole-3-butyric acid (IBA) and type of cuttings was examined on induction and growth of adventi- tious roots in Bambusa nutans Wall. and Bambusa tulda Roxb. Single- node culm and ...The influence of seasonal variation, indole-3-butyric acid (IBA) and type of cuttings was examined on induction and growth of adventi- tious roots in Bambusa nutans Wall. and Bambusa tulda Roxb. Single- node culm and culm-branch cuttings from the mature culms were pro- vided with immersion treatment for 24 h of either water (control) or 2 mM IBA in four different seasons, i.e., spring (mid February), summer (mid May), rainy (mid July), and winter (mid November) and maintained for two months in the mist chamber at the relative humidity of (70±5)% and the temperature of (30±2)oC. In B. nutans, adventitious rooting oc- curred in both types of cuttings in all the seasons with the best rooting in the summer season i.e., May (88% in culm cuttings) and the least in winter. On the contrary, adventitious rooting was recorded only in culm cuttings in spring and summer season in B. tulda. IBA treatment signifi- cantly enhanced rooting, root number and root length; registering 14 to 17 times improvement over control in the best rooting season. Three factor- interactions (season × cutting type × IBA treatment) were signifi- cant for rooting in B. nutans and all characteristics, except sprouting in B. tulda. Thus, single-node culm and culm-branch cuttings in B. nutans and culm cuttings in B. tulda treated with 2 mM IBA during spring (February) to summer (May) season are recommended for their clonal multiplication.展开更多
Biochemical changes associated with flowering in Bambusa arundinacea Linn and Bambusa nutans Wall.ex Munro were analyzed.Gregarious flowering was initiated in natural areas and plantations of B.arundinacea in late 201...Biochemical changes associated with flowering in Bambusa arundinacea Linn and Bambusa nutans Wall.ex Munro were analyzed.Gregarious flowering was initiated in natural areas and plantations of B.arundinacea in late 2014 and reached full bloom in early 2015,whereas sporadic flowering recorded during 2014–2015 in vegetatively propagated plants of B.nutans.Leaf and nodal shoot samples from flowering culms/shoots and nonflowering culms/shoots were collected in February 2015 at the initiation of fruit filling,then analyzed and compared for soluble sugars,phenols and peroxidase activity.In both species sugars were higher in leaves of flowering culms/shoots and lower in nodal shoots of flowering culms/shoots compared to the nonflowering.Phenols were lower in leaves and nodal shoots of flowering B.arundinacea,but higher in leaves and decreases in nodal shoots of B.nutans.Peroxidase activity increases in leaves and nodal shoots of B.arundinacea after flowering but increases in nodal shoots and decreases in leaves of B.nutans.展开更多
An investigation was conducted using ^(45)Ca as a radioactive tracer to evaluate calcium antagonistic effects of several extracts from Bambusa Rigida in living rats.The relationship between the flavonoid and saccharid...An investigation was conducted using ^(45)Ca as a radioactive tracer to evaluate calcium antagonistic effects of several extracts from Bambusa Rigida in living rats.The relationship between the flavonoid and saccharide contents of Bambusa Rigida and calcium antagonistic effects were also analyzed.The protective effects of the alkali extracts of Bambusa Rigida on myocardial ischemia were investigated in living rats.The results indicated that the alkali extracts of Bambusa Rigida had a prominent influence on Ca^(2+) influx and efflux in the isolated rat aorta and heart,as they could obviously block ^(45)Ca entering into cells and stimulate efflux of intracellular Ca^(2+).Moreover,the alkali extracts of Bambusa Rigida had favorable protective effects on myocardial ischemia induced either by isoproterenoi injection (ISO) or by the ligation of coronary artery.These results implied that the Bambusa Rigida had attractive potential for the treatment of heart,cerebrovascular and other diseases.However,the conclusion that whether the flavonoid or saccharide in Bambusa Rigida affected the calcium antagonistic effects and Ca^(2+) channels or not was hard to make within the results of the investigation.展开更多
[Objective]This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa♂. [Method]By using vertical polyacrylamide gel electrophor...[Objective]This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa♂. [Method]By using vertical polyacrylamide gel electrophoresis technology,three isozymes(LDH,EST,MDH) in nine tissues(heart,brain,eyes,liver,kidney,spleen,fin,muscle,plasma) of F1 hybrids of C. idellus ♀× E. bambusa ♂ were analyzed; differences in isozyme and proteins between F1 hybrids and parents were compared. [Result]Three isozymes in F1 hybrids of C. idellus ♀× E. bambusa ♂ exhibited varying degrees of tissue-specificity; isozymes and proteins in F1 hybrids were significantly different from the parents. [Conclusion]These nine differences could be used as indicators to identify F1 hybrids and parents.展开更多
文摘Plant of Bambusa vulgaris is used in traditional medicine practice for the management of many pathologies including hypertension. The effect of the aqueous extract of the leaves of Bambusa vulgaris on blood pressure was evaluated in normotensive male rabbits. Blood pressure was measured by the invasive method. Thus, aqueous extract of Bambusa vulgaris leaves had been injected intravenously at doses ranging from 1 mg/kg to 30 mg/kg in rabbits anesthetized with thiopental. The effects of this extract on blood pressure were also evaluated in rabbits having previously received different doses of atropine. The aqueous extract of Bambusa vulgaris leaves induced a dose-dependent hypotension which is not canceled in the presence of atropine. The results obtained show that the extract has blood pressure lowering effect which may be mediated by muscarinic-type cholinergic receptors.
基金Sichuan Science and Technology Program(2019YFG0139).
文摘Plants have evolved constitutive and inductive defense mechanisms to protect themselves from herbivorous insects.Metabolites in plants are thought to be involved in response to herbivores.Bambusa emeiensis is an important bamboo species widely distributed in Southwest China.It is easy to attract herbivores such as Cyrtotrachelus buqueti.Through the detection and analysis of metabolites in B.emeiensis metabolomic data,35 differential metabolites(biomarkers)were finally identified from 206 detection peaks,mainly primary metabolites.Subsequently,we conducted an association analysis between 35 biomarkers that annotated to be involved in 71 metabolic pathways in the metabolome and 324 differentially expressed genes(DEGs)in 207 gene expression pathways distributed in the transcriptome of B.emeiensis after feeding by C.buqueti.We also discussed the relationship between the changes in gene expression levels and metabolite content variation.A total of 47 integrated pathways containing the corresponding DEGs and biomarkers were screened out,with the amino acid synthesis pathway(ko01230)containing the most DEGs and biomarkers.In these integrated pathways,the changes in biomarkers content and the expression levels of the corresponding genes were generally consistent.For example,the increase in tryptophan content was accompanied by an increase in the expression of the corresponding catalase in the tryptophan synthesis pathway.Similar to this was glucose and trehalose in carbohydrate metabolism.Therefore,this study further deepened our understanding of the defense mechanism of B.emeiensis against bamboo pests and provided new insights for the prevention and control of bamboo diseases and pests.
基金supported by the National Key Research&Development Program of China(Grant No.2016YFD0600901)the National Natural Science Foundation of China(Grant No.31000294)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Dwindling supplies of bamboo resources and the increase in the demand for bamboo ha</span></span><span><span><span style="font-family:"">ve</span></span></span><span><span><span style="font-family:""> led to the need for better propagation techniques. This is the only way to ensure the sustainability of the bamboo resource base and improve livelihoods. Thus, this study was carried out to examine the different growth parameters of culm cuttings of <i>Bambusa vulgaris</i> in propagation beds with a substrate consisting of dark soil, manure (compost) and sand in a ratio of 1:1:1. Data on different growth parameters was collected on a daily basis over a three week period and analyzed using appropriate descriptive and inferential statistics. It was found that the different growth parameters (diameter, height, leaves and sheaths) of culm cuttings of <i>B. vulgaris</i> evolved in like manner in three propagation beds. Descriptive statistics for growth parameters of culm cuttings of <i>B. vulgaris</i> showed little or no differences across three shelter/propagation beds (shelter bed 1, shelter bed 2 and shelter bed 3) with 1.4675 ± 1.6172 cm, 93 ± 112 cm, 22 ± 30 leaves, and 4 ± 6 sheaths being the mean diameter, height, number of leaves and number of sheaths for all three propagation beds, respectively. ANOVA test showed no statistically significant variation (<i>p</i> > 0.05) between growth parameters of culm cuttings of <i>B. vulgaris</i> across the three propagation beds, implying that the different growth parameters were relatively similar. Pearson correlation showed the existence of direct and inverse relationships between the different growth parameters of culm cuttings of bamboo. The existence of these direct and inverse relationships could be attributed to the rapid growth rate of bamboo, the substrate used, and the species of bamboo used (<i>B. vulgaris</i>). The study recommends more large-scale propagation projects of this bamboo species in order to ensure the sustainability of the bamboo resource base and improve livelihoods of bamboo dependent rural communities.
文摘Bambusa vulgaris Schrad ex wendl is a widely cultivated bamboo species in rural Bangladesh for its versatile uses. The vegetative propagation becomes the only viable alternative for this species because B. vulgaris does not set seed after sparse flowering, which makes seedling progenies unavailable. A low-cost propagation trial was conducted to explore the clonal propagation techniques for the species with two types of small branch cuttings, nodal leafy cuttings and tip cuttings. The cuttings, after treating with 0, 0.1%, 0.4%, and 0.8% IBA solutions, were kept in non-mist propagator to let them to root for assessing the rooting ability. The cuttings were rooted in four weeks and were allowed to grow in the polybags for 10 months under nursery condition to assess their steckling capacity. The study reveals that both types of branch cuttings are able to develop roots, shoots, to survive and to form rhizome under the nursery condition. Rooting ability of the cuttings was significantly enhanced by the application of rooting hormone - IBA. The highest rooting percentage in nodal leafy cuttings and the tip cuttings (56.67% and 51.0%, respectively) were observed in 0.8% IBA treatment, followed by 0.4% IBA and the lowest (34.3% and 30.0%, respectively) was in control. The highest number of root developed per cutting (9.77 and 8.33 in nodal leafy cuttings and the tip cuttings, respectively) was also obtained from the cuttings treated with 0.8% IBA solution, followed 0.4% IBA treatment and the lowest (3.1 and 2.1, respectively) was in the cuttings without treatment. However, the length of the longest root varied significantly neither with the cutting types nor the concentrations of IBA solution. Survival percentage of the stecklings in nursery condition was significantly enhanced by IBA.
文摘Fermentation of the succulent bamboo shoots of Bambusa balcooa Roxb. resulted in an enrichment of phytosterols from 0.12% to 0.62% dry weight as compared to that of the fresh unfermented samples. The bacterial strains responsible for higher accumulation of phytosterols during fermentation of the bamboo shoots have been isolated and further extraction and purification of the crude phytosterols (isolated from the fermented samples) were done by TLC, UV, NMR, IR and Mass spectral analysis. The isolated phytosterols (β-sitos-terols) were then subjected to microbial transformation which yielded a considerable amount of androsta-1, 4-diene-3,17-dione (ADD) in the incubation mixture in presence of metabolic inhibitors (α, α＇-dipyridyl and sodium arsenate).
基金supported by Project of Commercial production of quality planting material of bamboo species(No.213/2014/Gen-1(NBM)(3)
文摘Season and concentration of sterilizing agents play a significant role for establishment of aseptic in vitro shoot cultures and sprouting of nodal explants from field growing culms of bamboo species.In the present investigation the nodal segment explants of Bambusa tulda Roxb collected in different seasons and treated with various concentrations of HgCl_2 showed significant variation in aseptic culture establishment and bud break.The rainy season(July–August) recorded with highest of 78% aseptic culture establishment whereas autumn recorded with lowest 46%.Summer and winter seasons emerged to be the best period, registering>60% in vitro bud break.On the other hand, the autumn season had the lowest value for bud break,i.e.42%.Among different doses of sterilizing agent tried,HgCl_2 0.1% found to be suitable for maximum aseptic culture establishment(66%) as well as bud break(59%).However, among the interactions, summer season and the dose of 0.1% HgCl_2 exhibited maximum of 73% response for both aseptic culture establishment and bud break.MS medium(liquid) enriched with 5.0 μM BA+5.0 μM Kn[Kinetin(N6-Furfuryladenine)] with additional supplementation of 100 μM glutamine+0.1 μM IAA supported a maximum in vitro shoot multiplication of 4.75 fold.The proliferated shoots were successfully rooted on MS medium(liquid) supplemented 40 μM coumarin.The plantlets transferred to the polythene bags showed 98% survival.
基金Supported by Wuhan Science and Technology Plan Projects(2013021001010464)
文摘[Objective] The purpose was to provide a reference for crossbreeding of grass carp. [Method]We conducted hybridization between Ctenopharyngodon idellus( ♀) and Elopichthys bambusa(♂) by artificial methods. The process of embryo and postembryonic development were observed and recorded. [Result]The fertilization rate,hatching rate and survival rate of the hybrid F1were( 75. 8 ± 6. 2) %,( 41. 9 ± 8. 2) % and( 9. 3 ± 3. 7) %,respectively. At the water temperature of 20. 1- 21. 6℃,the larvae was hatched for about 34 h and 25 min after fertilization. The whole length of newly-hatched larvae of the hybrid F1was( 5. 8 ± 0. 12) mm. The larvae could feed rotifer and unicellular algae after 3 or 4 days hatching. Postembryonic development of the hybrid at the formation of scales,lasted for 65 days when the young was( 74. 0 ± 2. 1) mm in whole length. [Conclusion]The embryonic development of hybrid F1 was intermediate to their parents,the form of the hybrid F1 was similar to that of their female line of grass carp. The growth rate of larva was faster than that of grass carp and similar to that of their paternal fish.
基金funded by Department of Biotechnology, Government of India, New Delhi(Sanction No.102/IFD/San 2632/2003-2004)
文摘The influence of seasonal variation, indole-3-butyric acid (IBA) and type of cuttings was examined on induction and growth of adventi- tious roots in Bambusa nutans Wall. and Bambusa tulda Roxb. Single- node culm and culm-branch cuttings from the mature culms were pro- vided with immersion treatment for 24 h of either water (control) or 2 mM IBA in four different seasons, i.e., spring (mid February), summer (mid May), rainy (mid July), and winter (mid November) and maintained for two months in the mist chamber at the relative humidity of (70±5)% and the temperature of (30±2)oC. In B. nutans, adventitious rooting oc- curred in both types of cuttings in all the seasons with the best rooting in the summer season i.e., May (88% in culm cuttings) and the least in winter. On the contrary, adventitious rooting was recorded only in culm cuttings in spring and summer season in B. tulda. IBA treatment signifi- cantly enhanced rooting, root number and root length; registering 14 to 17 times improvement over control in the best rooting season. Three factor- interactions (season × cutting type × IBA treatment) were signifi- cant for rooting in B. nutans and all characteristics, except sprouting in B. tulda. Thus, single-node culm and culm-branch cuttings in B. nutans and culm cuttings in B. tulda treated with 2 mM IBA during spring (February) to summer (May) season are recommended for their clonal multiplication.
基金carried out from research grant of Indian Council of Forestry Research and Education(ICFRE),Dehradun
文摘Biochemical changes associated with flowering in Bambusa arundinacea Linn and Bambusa nutans Wall.ex Munro were analyzed.Gregarious flowering was initiated in natural areas and plantations of B.arundinacea in late 2014 and reached full bloom in early 2015,whereas sporadic flowering recorded during 2014–2015 in vegetatively propagated plants of B.nutans.Leaf and nodal shoot samples from flowering culms/shoots and nonflowering culms/shoots were collected in February 2015 at the initiation of fruit filling,then analyzed and compared for soluble sugars,phenols and peroxidase activity.In both species sugars were higher in leaves of flowering culms/shoots and lower in nodal shoots of flowering culms/shoots compared to the nonflowering.Phenols were lower in leaves and nodal shoots of flowering B.arundinacea,but higher in leaves and decreases in nodal shoots of B.nutans.Peroxidase activity increases in leaves and nodal shoots of B.arundinacea after flowering but increases in nodal shoots and decreases in leaves of B.nutans.
基金National Natural Science Foundation of China (Grant No.10175044).
文摘An investigation was conducted using ^(45)Ca as a radioactive tracer to evaluate calcium antagonistic effects of several extracts from Bambusa Rigida in living rats.The relationship between the flavonoid and saccharide contents of Bambusa Rigida and calcium antagonistic effects were also analyzed.The protective effects of the alkali extracts of Bambusa Rigida on myocardial ischemia were investigated in living rats.The results indicated that the alkali extracts of Bambusa Rigida had a prominent influence on Ca^(2+) influx and efflux in the isolated rat aorta and heart,as they could obviously block ^(45)Ca entering into cells and stimulate efflux of intracellular Ca^(2+).Moreover,the alkali extracts of Bambusa Rigida had favorable protective effects on myocardial ischemia induced either by isoproterenoi injection (ISO) or by the ligation of coronary artery.These results implied that the Bambusa Rigida had attractive potential for the treatment of heart,cerebrovascular and other diseases.However,the conclusion that whether the flavonoid or saccharide in Bambusa Rigida affected the calcium antagonistic effects and Ca^(2+) channels or not was hard to make within the results of the investigation.
基金Supported by Project of Project of Science and Technology of Wuhan City(2013021001010464)
文摘[Objective]This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa♂. [Method]By using vertical polyacrylamide gel electrophoresis technology,three isozymes(LDH,EST,MDH) in nine tissues(heart,brain,eyes,liver,kidney,spleen,fin,muscle,plasma) of F1 hybrids of C. idellus ♀× E. bambusa ♂ were analyzed; differences in isozyme and proteins between F1 hybrids and parents were compared. [Result]Three isozymes in F1 hybrids of C. idellus ♀× E. bambusa ♂ exhibited varying degrees of tissue-specificity; isozymes and proteins in F1 hybrids were significantly different from the parents. [Conclusion]These nine differences could be used as indicators to identify F1 hybrids and parents.
