Bloom’s taxonomy is widely used in educational research to categorize the cognitive skills required to answer exam questions.For this study,we analyzed how students categorize exam questions(high-level question or lo...Bloom’s taxonomy is widely used in educational research to categorize the cognitive skills required to answer exam questions.For this study,we analyzed how students categorize exam questions(high-level question or low-level question,)gathered data as to their rationale for categorization,and compared their categorizations to those of experts.We found that students consistently rank high-level questions incorrectly.We analyzed student reasons for their categorizations,and found that for many of the incorrectly categorized questions the students referred to reasons related to Cognitive Load Theory.This shows that cognitive load prevents students from accurately assessing the cognitive level of an exam question.Thus,extra cognitive load in exam questions may prevent those questions from accurately measuring the skills and knowledge of the student.This points to the need for instructors to eliminate cognitive load from their exams.展开更多
Assessment using Bloom’s taxonomy levels has evolved in a variety of contexts and uses.In the era of the COVID-19 pandemic,which necessitates use of online assessment,the need for teachers to use digital-based taxono...Assessment using Bloom’s taxonomy levels has evolved in a variety of contexts and uses.In the era of the COVID-19 pandemic,which necessitates use of online assessment,the need for teachers to use digital-based taxonomy skills or Bloom’s Digital Taxonomy(BDT)has increased even more.However,the existing studies on validity and reliability of BDT items are limited.To overcome this limitation,this study aims to test whether BDT has good psychometric characteristics as a teacher’s self-assessment tool using the Rasch model analysis and to investigate the pattern of BDT usage in teaching and learning.By using a quantitative online survey design,this study involves six levels of BDT,namely,Remembering,Understanding,Applying,Analyzing,Evaluating,and Creating.The questionnaire was developed and validated by two experts prior to administration.A stratified random sampling technique was conducted on 774 secondary teachers from five geographical zones in Malaysia,and the Rasch model was analyzed using WINSTEPS 3.71 software.The performances of items improved by Rasch psychometric assessment including the application of BDT among teachers.The hierarchy level was also assessed through graphical analysis,including the Wright map and bubble chart,to demonstrate the powerful performance of the Rasch model analysis in investigating item quality and reliability.Overall,these empirically validated items using the Rasch model could advance the academic knowledge of BDT for future assessment and promote the Rasch calibration in an educational setting.展开更多
While prokaryotes play key roles in nutrient cycling and energy flow during Phaeocystis globosa blooms,the information on the spatial and diel temporal distribution of the prokaryotic community during Phaeocystis bloo...While prokaryotes play key roles in nutrient cycling and energy flow during Phaeocystis globosa blooms,the information on the spatial and diel temporal distribution of the prokaryotic community during Phaeocystis blooms remains scarce.In January 2019,we used high-throughput sequencing of the 16S rRNA gene to explore the spatial and diel variations of particle-attached(PA)and free-living(FL)prokaryotic communities during the blooming phase of P.globosa in Beibu Gulf,Guangxi,China.The results suggested a significant spatial variation pattern in the horizontal distribution of prokaryotic communities,while there was no significant difference in the vertical direction.Both spatial distance and environmental variables shaped the horizontal distribution of the prokaryotic community structure,while environmental variables,particularly the abundance of P.globosa colony and Chl a,showed more significant influence and were closely related to the structure and variation of the prokaryotic community.Strong vertical mixing of the water column disrupted the vertical structure heterogeneity of the prokaryotic community in winter.There were significant differences in the diel samples of PA prokaryotic communities,but not in the FL prokaryotic communities.Nitrate,ammonium and the abundance of P.globosa colony were the key environmental variables impacting the diel variations of prokaryotic communities over the sampling period.The present study provided valuable information to depict the spatial-temporal variations of the microbial community and its association with environmental parameters during P.globosa bloom in the tropical gulf.展开更多
The majority of recent studies on prevention and control of childhood obesity focus on methods for achieving weight loss. In contrast, the Health at Every Size (HAES) paradigm fosters improved health behaviors for peo...The majority of recent studies on prevention and control of childhood obesity focus on methods for achieving weight loss. In contrast, the Health at Every Size (HAES) paradigm fosters improved health behaviors for people of all sizes by emphasizing natural diversity of body type and attention to social, emotional, and spiritual factors in addition to physical ones. This study examined introduction of the HAES paradigm to student teachers using different communication models while moving across the learning domains of Bloom’s Taxonomy. The qualitative research tested a face-to-face verbal communication model and a Computer-Mediated Communication (CMC) module and its blog. It was also based upon reflection diaries recorded by participants using both models. Participants included Druze, Jewish, and Bedouin students at a teacher training program in northern Israel. The student teachers succeeded in developing the HAES concepts further while demonstrating the higher domain levels across Bloom’s Taxonomy. These higher levels emerged when engaging with theoretical concepts and practical dilemmas relating to the HAES concepts of health promotion, body image, well-being, and self-worth. Challenging the appreciation of HAES using different communication models can be translated into diverse contexts of Health Education Practices.展开更多
In this paper, Law of Conservation of Mass （＂The Law＂）, one content standard of junior middle school chemistry, was decomposed based on The Revision of Bloom＇s Taxonomy of Educational Objectives （＂The Revision...In this paper, Law of Conservation of Mass （＂The Law＂）, one content standard of junior middle school chemistry, was decomposed based on The Revision of Bloom＇s Taxonomy of Educational Objectives （＂The Revision＂） and placed in the classification table. In order to understand the teaching objectives more, the relationship between the related activities was studied. Teachers can design the teaching plans based on teaching objective, and determine different evaluations method according to different types of teaching objectives. It will raise students more cognitive activities and promote targeted objectives teaching.展开更多
This project is a study of Bloom’s taxonomy in mathematics. Two groups of 7th Grade students were used to carry out the study, each undertaking a different set of tests at three different levels;the standard traditio...This project is a study of Bloom’s taxonomy in mathematics. Two groups of 7th Grade students were used to carry out the study, each undertaking a different set of tests at three different levels;the standard traditional test format and the new test based on Bloom’s taxonomy format. The study was aimed at comparing the differences in the outcomes between the two test formats based on the mathematics performances of the students. This study shows how Bloom’s taxonomy can be utilized to develop a clear methodology for creating a test direction when compiling reports and in conducting an experimental assessment of students’ performance.展开更多
Bloom＇s syndrome is a rare disease that is related to an increased risk of developing malignant neoplasm. The patient reported was followed up with several hospital staff when she was diagnosed with gastric cancer, b...Bloom＇s syndrome is a rare disease that is related to an increased risk of developing malignant neoplasm. The patient reported was followed up with several hospital staff when she was diagnosed with gastric cancer, but unfortunately, she had already peritoneal implants in moment of the surgery approach. It has been reported in the literature that patients with this syndrome present greater toxicity to the chemotherapeutic treatment, however due to the rarity of the disease, it is not known exactly how the adjustment of these drugs should be performed and which is the better protocol to use. Palliative chemotherapy was proposed, but after receiving one dose of the initially treatment, she developed severe and limiting toxicity.展开更多
Cyanobacterial harmful algal blooms are a major threat to freshwater eco-systems globally. To deal with this threat, researches into the cyanobacteria bloom in fresh water lakes and rivers have been carried out all ov...Cyanobacterial harmful algal blooms are a major threat to freshwater eco-systems globally. To deal with this threat, researches into the cyanobacteria bloom in fresh water lakes and rivers have been carried out all over the world. This review presents an overlook of studies on cyanobacteria blooms. Conventional studies mainly focus on investigating the environmental factors influencing the blooms, with their limitation in lack of viewing the microbial community structures. Metagenomics study provides insight into the internal community structure of the cyanobacteria at the blooming, and there are researchers reported that sequence data was a better predictor than environmental factors. This further manifests the significance of the metagenomic study. However, large number of the latter appears to be confined only to present snapshoot of the microbial community diversity and structure. This type of investigation has been valuable and important, whilst an effort to integrate and coordinate the conventional approaches that largely focus on the environmental factors control, and the Metagenomics approaches that reveals the microbial community structure and diversity, implemented through machine learning techniques, for a holistic and more comprehensive insight into the cause and control of Cyanobacteria blooms, appear to be a trend and challenge of the study of this field.展开更多
For many control systems in real life, impulses and delays are intrinsic phenomena that do not modify their controllability. So we conjecture that under certain conditions the abrupt changes and delays as perturbation...For many control systems in real life, impulses and delays are intrinsic phenomena that do not modify their controllability. So we conjecture that under certain conditions the abrupt changes and delays as perturbations of a system do not destroy its controllability. There are many practical examples of impulsive control systems with delays, such as a chemical reactor system, a financial system with two state variables, the amount of money in a market and the savings rate of a central bank, and the growth of a population diffusing throughout its habitat modeled by a reaction-diffusion equation. In this paper we apply the Rothe’s Fixed Point Theorem to prove the interior approximate controllability of the following Benjamin Bona-Mohany(BBM) type equation with impulses and delay where and are constants, Ω is a domain in , ω is an open non-empty subset of Ω , denotes the characteristic function of the set ω , the distributed control , are continuous functions and the nonlinear functions are smooth enough functions satisfying some additional conditions.展开更多
This paper presents the improvements on equipment and process technologies of Baosteel EAF Bloom Caster Production Line.By using the new VOD equipment,super low carbon super 13Cr was successfully made;By applying and ...This paper presents the improvements on equipment and process technologies of Baosteel EAF Bloom Caster Production Line.By using the new VOD equipment,super low carbon super 13Cr was successfully made;By applying and developing the Soft Reduction technology,very good results were achieved on high quality steel grades such as tire cord steel,bear steel,hi-alloy steel;the ladle Anti-Slag Detector is normally applied,based on the detail analysis during the performance test period,the Detector gives out slag entrapment alarms 7-9 s average before slag flows into tundish.By optimizing production control points of each key process,great achievements are realized on high quality steel grades such as tire cord steel,bear steel,hi-alloy steel and hi-S/Al steel,etc.Some achievements and technical indices are presented,too.展开更多
Based on B.S.Bloom's Taxonomy for affective learning and teaching,an overview of how to apply affective teaching into English teaching is demonstrated.It is argued that to cultivate students'affective characte...Based on B.S.Bloom's Taxonomy for affective learning and teaching,an overview of how to apply affective teaching into English teaching is demonstrated.It is argued that to cultivate students'affective characteristics should be an indispensable objective in college English teaching.展开更多
Online English learning as an outcome of the rapid development of the Internet has got a wider and wider market in China. However, problems of varieties have also occurred along its way. People never stop thinking of ...Online English learning as an outcome of the rapid development of the Internet has got a wider and wider market in China. However, problems of varieties have also occurred along its way. People never stop thinking of better strategies either in designing online course wares or tutorials to help smooth the learning process. My experience as a tutor is that interaction of affective domain and higher levels of cognitive domain of Bloom＇s Taxonomy plays an important role in face-to-face tutorials of online English learning.展开更多
This study aimed to develop an educational model that integrates three elements: knowledge, skills, and attitudes—developing the educational model proposed in the previous Paper I—and to widely investigate and chara...This study aimed to develop an educational model that integrates three elements: knowledge, skills, and attitudes—developing the educational model proposed in the previous Paper I—and to widely investigate and characterize previous learning-related models. The basic educational model proposed here is my seven-step process model of rehabilitation practice. Knowledge consists of four aspects: 1) clinical, 2) psychological, 3) environmental, and 4) disability;skills consist of two steps: 5) identifying intervention points and 6) setting feasible goals;and attitudes 7) of communicating and sharing policies and paths with patients, families, and other professionals. This constitutes the process of rehabilitation practice, and a framework that integrates the three elements is developed here. This study focuses on integrating knowledge, skills, and attitudes into what Bloom described as “the integration of instruction and assessment” so that learners and instructors can reconcile them. Therefore, a typology that explains each other for advancing and deepening individual skills is adopted. In Bloom’s original taxonomy of educational goals, the cognitive domain has five layers in the pyramid of knowledge;the psychomotor domain of Simpson’s has seven layers, and Bloom’s affective domain is represented by five in another pyramid. In addition, the above seven layers of the process model and the seven layers of the skill level of the Dreyfus model were brought together. The integration of the above five typologies becomes a useful educational evaluation model when the relationships are clarified.展开更多
This study aimed to develop an educational model that integrates three elements: knowledge, skills, and attitudes—developing the educational model proposed in the previous Paper I—and to widely investigate and chara...This study aimed to develop an educational model that integrates three elements: knowledge, skills, and attitudes—developing the educational model proposed in the previous Paper I—and to widely investigate and characterize previous learning-related models. The basic educational model proposed here is my seven-step process model of rehabilitation practice. Knowledge consists of four aspects: 1) clinical, 2) psychological, 3) environmental, and 4) disability;skills consist of two steps: 5) identifying intervention points and 6) setting feasible goals;and attitudes 7) of communicating and sharing policies and paths with patients, families, and other professionals. This constitutes the process of rehabilitation practice, and a framework that integrates the three elements is developed here. This study focuses on integrating knowledge, skills, and attitudes into what Bloom described as “the integration of instruction and assessment” so that learners and instructors can reconcile them. Therefore, a typology that explains each other for advancing and deepening individual skills is adopted. In Bloom’s original taxonomy of educational goals, the cognitive domain has five layers in the pyramid of knowledge;the psychomotor domain of Simpson’s has seven layers, and Bloom’s affective domain is represented by five in another pyramid. In addition, the above seven layers of the process model and the seven layers of the skill level of the Dreyfus model were brought together. The integration of the above five typologies becomes a useful educational evaluation model when the relationships are clarified.展开更多
Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacte...Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacterium B5 was isolated from activated sludge. On the basis of analysis of its physiological characteristics and 16S rDNA gene sequence, it was identified as Bacillusfusiformis. Its algaelysing characteristics on Microcystis aeruginosa, Chlorella and Scenedesmus were tested. The results showed that： （1） the algicidal bacterium B5 is a Gram-negative bacterium. The 16S rDNA nucleotide sequence homology of strain B5 with 2 strains of B. fusiformis reached 99.86%, so B5 was identified as B. fusiformis; （2） the algal-lysing effects of the algicidal bacterium B5 on M. aeruginosa, Chlorella and Scenedesrnus were pronounced. The initial bacterial and algal cell densities strongly influence the removal rates of chlorophyll-a. The greater the initial bacterial cell density, the faster the degradation of chlorophyll-a. The greater the initial algal cell density, the slower the degradation of chlorophyll-a. When the bacterial cell density was 3.6 × 10^7 cells/ml, nearly 90% of chlorophyll-a was removed. When the chlorophyll-a concentration was less than 550 μg/L, about 70% was removed; （3） the strain B5 lysed algae by secreting metabolites and these metabolites could bear heat treatment.展开更多
Over the past decades,jellyfish occurred increasingly and abundantly in coastal areas worldwide.Usually,biomass of jellyfish,especially when they bloom,can be determined by visual counting.However,tiny individuals of ...Over the past decades,jellyfish occurred increasingly and abundantly in coastal areas worldwide.Usually,biomass of jellyfish,especially when they bloom,can be determined by visual counting.However,tiny individuals of jellyfish(e.g.,planulae,polyps,and ephyrae)are difficult to detect in the field.In this study,species-specific quantitative real-time PCR assays(qPCR)(SYBR Green I)targeting the mitochondrial 16S rDNA(mt-16S rDNA)of jellyfish were developed and were used to estimate the distribution and seasonal fluctuations of four jellyfish species(Nemopilema nomurai,Cyanea nozakii,Rhopilema esculentum,and Aurelia coerulea)in Jiaozhou Bay(JZB),China in 2013.The mt-16S rDNA of A.coerulea and N.nomurai was detected in most of the survey months and it peaked in July(1.03×10^(4) copies/L)and September(1.08×10^(6) copies/L),respectively.The mt-16S rDNA of C.nozakii occurred from August to October only with monthly mean values of 7.18-46.17 copies/L and was mainly located from the middle part to the outer part of the bay.The mt-16S rDNA of R.esculentum was the least abundant among the four species and was detected in only one sample(B2 station in March),with a value of 88.49 copies/L.The Spearman correlation test revealed that phytoplankton biomass was significantly and positively correlated with the mt-16S rDNA abundance of A.coerulea(R=0.37,P<0.01)and negatively with the mt-16S rDNA of N.nomurai(R=-0.36,P<0.01).The qPCR assay will enable the identification and quantification of jellyfish species in their whole life history and can be used as an approach in combination of the traditional jellyfish survey.展开更多
The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individ...The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individuals, such as planulae. To solve this problem, we developed a real-time PCR assay （SYBR Green I） to identify planulae in both cultured and natural seawater samples. Species-specific primers targeting Aurelia sp.1 mitochondrial 16S rDNA （mr 16S rDNA） regions were designed. Using a calibration curve constructed with plasmids containing the Aurelia sp. 1 mt 16S rDNA fragment and a standard curve for planulae, the absolute number of mt 16S rDNA copies per planula was determined and from that the total number ofplanulae per sample was estimated. For the field samples, a 100-fold dilution of the sample DNA combined with a final concentration of 0.2 μg/μL BSA in the PCR reaction mixture was used to remove real- time PCR inhibitors. Samples collected in Jiaozhou Bay from July to September 2012 were subsequently analyzed using this assay. Peak Aurelia sp.1 planula abundance occurred in July 2012 at stations near Hongdao Island and Qingdao offshore; abundances were very low in August and September. The real-time PCR assay （SYBR Green I） developed here negates the need for traditional microscopic identification, which is laborious and time-consuming, and can detect and quantify jellyfish planulae in field plankton samples rapidly and specifically.展开更多
文摘Bloom’s taxonomy is widely used in educational research to categorize the cognitive skills required to answer exam questions.For this study,we analyzed how students categorize exam questions(high-level question or low-level question,)gathered data as to their rationale for categorization,and compared their categorizations to those of experts.We found that students consistently rank high-level questions incorrectly.We analyzed student reasons for their categorizations,and found that for many of the incorrectly categorized questions the students referred to reasons related to Cognitive Load Theory.This shows that cognitive load prevents students from accurately assessing the cognitive level of an exam question.Thus,extra cognitive load in exam questions may prevent those questions from accurately measuring the skills and knowledge of the student.This points to the need for instructors to eliminate cognitive load from their exams.
基金funded by the Ministry of Higher Education(MOHE),Malaysia under the Fundamental Research Grant Scheme(FRGS)(FRGS/1/2018/SSI09/UKM/02/1)the Faculty of Education,University Kebangsaan Malaysia(UKM),Geran Galakan Penyelidik Muda(GGPM-2017-088).
文摘Assessment using Bloom’s taxonomy levels has evolved in a variety of contexts and uses.In the era of the COVID-19 pandemic,which necessitates use of online assessment,the need for teachers to use digital-based taxonomy skills or Bloom’s Digital Taxonomy(BDT)has increased even more.However,the existing studies on validity and reliability of BDT items are limited.To overcome this limitation,this study aims to test whether BDT has good psychometric characteristics as a teacher’s self-assessment tool using the Rasch model analysis and to investigate the pattern of BDT usage in teaching and learning.By using a quantitative online survey design,this study involves six levels of BDT,namely,Remembering,Understanding,Applying,Analyzing,Evaluating,and Creating.The questionnaire was developed and validated by two experts prior to administration.A stratified random sampling technique was conducted on 774 secondary teachers from five geographical zones in Malaysia,and the Rasch model was analyzed using WINSTEPS 3.71 software.The performances of items improved by Rasch psychometric assessment including the application of BDT among teachers.The hierarchy level was also assessed through graphical analysis,including the Wright map and bubble chart,to demonstrate the powerful performance of the Rasch model analysis in investigating item quality and reliability.Overall,these empirically validated items using the Rasch model could advance the academic knowledge of BDT for future assessment and promote the Rasch calibration in an educational setting.
基金The National Key Research and Development Program of the Ministry of Science and Technology,China,under contract No.2017YFC1404300the Scientific and Technological Innovation Project of the Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2016ASKJ02the Aoshan Talents Program of the Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2015ASTP.
文摘While prokaryotes play key roles in nutrient cycling and energy flow during Phaeocystis globosa blooms,the information on the spatial and diel temporal distribution of the prokaryotic community during Phaeocystis blooms remains scarce.In January 2019,we used high-throughput sequencing of the 16S rRNA gene to explore the spatial and diel variations of particle-attached(PA)and free-living(FL)prokaryotic communities during the blooming phase of P.globosa in Beibu Gulf,Guangxi,China.The results suggested a significant spatial variation pattern in the horizontal distribution of prokaryotic communities,while there was no significant difference in the vertical direction.Both spatial distance and environmental variables shaped the horizontal distribution of the prokaryotic community structure,while environmental variables,particularly the abundance of P.globosa colony and Chl a,showed more significant influence and were closely related to the structure and variation of the prokaryotic community.Strong vertical mixing of the water column disrupted the vertical structure heterogeneity of the prokaryotic community in winter.There were significant differences in the diel samples of PA prokaryotic communities,but not in the FL prokaryotic communities.Nitrate,ammonium and the abundance of P.globosa colony were the key environmental variables impacting the diel variations of prokaryotic communities over the sampling period.The present study provided valuable information to depict the spatial-temporal variations of the microbial community and its association with environmental parameters during P.globosa bloom in the tropical gulf.
文摘The majority of recent studies on prevention and control of childhood obesity focus on methods for achieving weight loss. In contrast, the Health at Every Size (HAES) paradigm fosters improved health behaviors for people of all sizes by emphasizing natural diversity of body type and attention to social, emotional, and spiritual factors in addition to physical ones. This study examined introduction of the HAES paradigm to student teachers using different communication models while moving across the learning domains of Bloom’s Taxonomy. The qualitative research tested a face-to-face verbal communication model and a Computer-Mediated Communication (CMC) module and its blog. It was also based upon reflection diaries recorded by participants using both models. Participants included Druze, Jewish, and Bedouin students at a teacher training program in northern Israel. The student teachers succeeded in developing the HAES concepts further while demonstrating the higher domain levels across Bloom’s Taxonomy. These higher levels emerged when engaging with theoretical concepts and practical dilemmas relating to the HAES concepts of health promotion, body image, well-being, and self-worth. Challenging the appreciation of HAES using different communication models can be translated into diverse contexts of Health Education Practices.
文摘In this paper, Law of Conservation of Mass （＂The Law＂）, one content standard of junior middle school chemistry, was decomposed based on The Revision of Bloom＇s Taxonomy of Educational Objectives （＂The Revision＂） and placed in the classification table. In order to understand the teaching objectives more, the relationship between the related activities was studied. Teachers can design the teaching plans based on teaching objective, and determine different evaluations method according to different types of teaching objectives. It will raise students more cognitive activities and promote targeted objectives teaching.
文摘This project is a study of Bloom’s taxonomy in mathematics. Two groups of 7th Grade students were used to carry out the study, each undertaking a different set of tests at three different levels;the standard traditional test format and the new test based on Bloom’s taxonomy format. The study was aimed at comparing the differences in the outcomes between the two test formats based on the mathematics performances of the students. This study shows how Bloom’s taxonomy can be utilized to develop a clear methodology for creating a test direction when compiling reports and in conducting an experimental assessment of students’ performance.
文摘Bloom＇s syndrome is a rare disease that is related to an increased risk of developing malignant neoplasm. The patient reported was followed up with several hospital staff when she was diagnosed with gastric cancer, but unfortunately, she had already peritoneal implants in moment of the surgery approach. It has been reported in the literature that patients with this syndrome present greater toxicity to the chemotherapeutic treatment, however due to the rarity of the disease, it is not known exactly how the adjustment of these drugs should be performed and which is the better protocol to use. Palliative chemotherapy was proposed, but after receiving one dose of the initially treatment, she developed severe and limiting toxicity.
文摘Cyanobacterial harmful algal blooms are a major threat to freshwater eco-systems globally. To deal with this threat, researches into the cyanobacteria bloom in fresh water lakes and rivers have been carried out all over the world. This review presents an overlook of studies on cyanobacteria blooms. Conventional studies mainly focus on investigating the environmental factors influencing the blooms, with their limitation in lack of viewing the microbial community structures. Metagenomics study provides insight into the internal community structure of the cyanobacteria at the blooming, and there are researchers reported that sequence data was a better predictor than environmental factors. This further manifests the significance of the metagenomic study. However, large number of the latter appears to be confined only to present snapshoot of the microbial community diversity and structure. This type of investigation has been valuable and important, whilst an effort to integrate and coordinate the conventional approaches that largely focus on the environmental factors control, and the Metagenomics approaches that reveals the microbial community structure and diversity, implemented through machine learning techniques, for a holistic and more comprehensive insight into the cause and control of Cyanobacteria blooms, appear to be a trend and challenge of the study of this field.
文摘For many control systems in real life, impulses and delays are intrinsic phenomena that do not modify their controllability. So we conjecture that under certain conditions the abrupt changes and delays as perturbations of a system do not destroy its controllability. There are many practical examples of impulsive control systems with delays, such as a chemical reactor system, a financial system with two state variables, the amount of money in a market and the savings rate of a central bank, and the growth of a population diffusing throughout its habitat modeled by a reaction-diffusion equation. In this paper we apply the Rothe’s Fixed Point Theorem to prove the interior approximate controllability of the following Benjamin Bona-Mohany(BBM) type equation with impulses and delay where and are constants, Ω is a domain in , ω is an open non-empty subset of Ω , denotes the characteristic function of the set ω , the distributed control , are continuous functions and the nonlinear functions are smooth enough functions satisfying some additional conditions.
文摘This paper presents the improvements on equipment and process technologies of Baosteel EAF Bloom Caster Production Line.By using the new VOD equipment,super low carbon super 13Cr was successfully made;By applying and developing the Soft Reduction technology,very good results were achieved on high quality steel grades such as tire cord steel,bear steel,hi-alloy steel;the ladle Anti-Slag Detector is normally applied,based on the detail analysis during the performance test period,the Detector gives out slag entrapment alarms 7-9 s average before slag flows into tundish.By optimizing production control points of each key process,great achievements are realized on high quality steel grades such as tire cord steel,bear steel,hi-alloy steel and hi-S/Al steel,etc.Some achievements and technical indices are presented,too.
文摘Based on B.S.Bloom's Taxonomy for affective learning and teaching,an overview of how to apply affective teaching into English teaching is demonstrated.It is argued that to cultivate students'affective characteristics should be an indispensable objective in college English teaching.
文摘Online English learning as an outcome of the rapid development of the Internet has got a wider and wider market in China. However, problems of varieties have also occurred along its way. People never stop thinking of better strategies either in designing online course wares or tutorials to help smooth the learning process. My experience as a tutor is that interaction of affective domain and higher levels of cognitive domain of Bloom＇s Taxonomy plays an important role in face-to-face tutorials of online English learning.
文摘This study aimed to develop an educational model that integrates three elements: knowledge, skills, and attitudes—developing the educational model proposed in the previous Paper I—and to widely investigate and characterize previous learning-related models. The basic educational model proposed here is my seven-step process model of rehabilitation practice. Knowledge consists of four aspects: 1) clinical, 2) psychological, 3) environmental, and 4) disability;skills consist of two steps: 5) identifying intervention points and 6) setting feasible goals;and attitudes 7) of communicating and sharing policies and paths with patients, families, and other professionals. This constitutes the process of rehabilitation practice, and a framework that integrates the three elements is developed here. This study focuses on integrating knowledge, skills, and attitudes into what Bloom described as “the integration of instruction and assessment” so that learners and instructors can reconcile them. Therefore, a typology that explains each other for advancing and deepening individual skills is adopted. In Bloom’s original taxonomy of educational goals, the cognitive domain has five layers in the pyramid of knowledge;the psychomotor domain of Simpson’s has seven layers, and Bloom’s affective domain is represented by five in another pyramid. In addition, the above seven layers of the process model and the seven layers of the skill level of the Dreyfus model were brought together. The integration of the above five typologies becomes a useful educational evaluation model when the relationships are clarified.
文摘This study aimed to develop an educational model that integrates three elements: knowledge, skills, and attitudes—developing the educational model proposed in the previous Paper I—and to widely investigate and characterize previous learning-related models. The basic educational model proposed here is my seven-step process model of rehabilitation practice. Knowledge consists of four aspects: 1) clinical, 2) psychological, 3) environmental, and 4) disability;skills consist of two steps: 5) identifying intervention points and 6) setting feasible goals;and attitudes 7) of communicating and sharing policies and paths with patients, families, and other professionals. This constitutes the process of rehabilitation practice, and a framework that integrates the three elements is developed here. This study focuses on integrating knowledge, skills, and attitudes into what Bloom described as “the integration of instruction and assessment” so that learners and instructors can reconcile them. Therefore, a typology that explains each other for advancing and deepening individual skills is adopted. In Bloom’s original taxonomy of educational goals, the cognitive domain has five layers in the pyramid of knowledge;the psychomotor domain of Simpson’s has seven layers, and Bloom’s affective domain is represented by five in another pyramid. In addition, the above seven layers of the process model and the seven layers of the skill level of the Dreyfus model were brought together. The integration of the above five typologies becomes a useful educational evaluation model when the relationships are clarified.
基金Project supported by the Special Funds for Doctor's Station of University(No.20060246024)Young Fund of Fudan University,and the Shanghai Tongji Gao Tingyao Environmental Science and Technology Developmem Fundation
文摘Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacterium B5 was isolated from activated sludge. On the basis of analysis of its physiological characteristics and 16S rDNA gene sequence, it was identified as Bacillusfusiformis. Its algaelysing characteristics on Microcystis aeruginosa, Chlorella and Scenedesmus were tested. The results showed that： （1） the algicidal bacterium B5 is a Gram-negative bacterium. The 16S rDNA nucleotide sequence homology of strain B5 with 2 strains of B. fusiformis reached 99.86%, so B5 was identified as B. fusiformis; （2） the algal-lysing effects of the algicidal bacterium B5 on M. aeruginosa, Chlorella and Scenedesrnus were pronounced. The initial bacterial and algal cell densities strongly influence the removal rates of chlorophyll-a. The greater the initial bacterial cell density, the faster the degradation of chlorophyll-a. The greater the initial algal cell density, the slower the degradation of chlorophyll-a. When the bacterial cell density was 3.6 × 10^7 cells/ml, nearly 90% of chlorophyll-a was removed. When the chlorophyll-a concentration was less than 550 μg/L, about 70% was removed; （3） the strain B5 lysed algae by secreting metabolites and these metabolites could bear heat treatment.
基金Supported by the National Key Research and Development Program of China(Nos.2017YFC1404404,2017YFC1404402)the National Natural Science Foundation of China(No.41906112)the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology(No.2016ASKJ02)。
文摘Over the past decades,jellyfish occurred increasingly and abundantly in coastal areas worldwide.Usually,biomass of jellyfish,especially when they bloom,can be determined by visual counting.However,tiny individuals of jellyfish(e.g.,planulae,polyps,and ephyrae)are difficult to detect in the field.In this study,species-specific quantitative real-time PCR assays(qPCR)(SYBR Green I)targeting the mitochondrial 16S rDNA(mt-16S rDNA)of jellyfish were developed and were used to estimate the distribution and seasonal fluctuations of four jellyfish species(Nemopilema nomurai,Cyanea nozakii,Rhopilema esculentum,and Aurelia coerulea)in Jiaozhou Bay(JZB),China in 2013.The mt-16S rDNA of A.coerulea and N.nomurai was detected in most of the survey months and it peaked in July(1.03×10^(4) copies/L)and September(1.08×10^(6) copies/L),respectively.The mt-16S rDNA of C.nozakii occurred from August to October only with monthly mean values of 7.18-46.17 copies/L and was mainly located from the middle part to the outer part of the bay.The mt-16S rDNA of R.esculentum was the least abundant among the four species and was detected in only one sample(B2 station in March),with a value of 88.49 copies/L.The Spearman correlation test revealed that phytoplankton biomass was significantly and positively correlated with the mt-16S rDNA abundance of A.coerulea(R=0.37,P<0.01)and negatively with the mt-16S rDNA of N.nomurai(R=-0.36,P<0.01).The qPCR assay will enable the identification and quantification of jellyfish species in their whole life history and can be used as an approach in combination of the traditional jellyfish survey.
基金Supported by the National Basic Research Program of China(973 Program)(No.2011CB403602)the National Natural Science Foundation of China(No.41076085)the National Special Research Fund for Non-Profit Marine Sector(No.201205031)
文摘The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individuals, such as planulae. To solve this problem, we developed a real-time PCR assay （SYBR Green I） to identify planulae in both cultured and natural seawater samples. Species-specific primers targeting Aurelia sp.1 mitochondrial 16S rDNA （mr 16S rDNA） regions were designed. Using a calibration curve constructed with plasmids containing the Aurelia sp. 1 mt 16S rDNA fragment and a standard curve for planulae, the absolute number of mt 16S rDNA copies per planula was determined and from that the total number ofplanulae per sample was estimated. For the field samples, a 100-fold dilution of the sample DNA combined with a final concentration of 0.2 μg/μL BSA in the PCR reaction mixture was used to remove real- time PCR inhibitors. Samples collected in Jiaozhou Bay from July to September 2012 were subsequently analyzed using this assay. Peak Aurelia sp.1 planula abundance occurred in July 2012 at stations near Hongdao Island and Qingdao offshore; abundances were very low in August and September. The real-time PCR assay （SYBR Green I） developed here negates the need for traditional microscopic identification, which is laborious and time-consuming, and can detect and quantify jellyfish planulae in field plankton samples rapidly and specifically.
