Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owi...Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation.Herein,by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer,we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP.This nanoprobe reveals a very low fluorescence intensity because of the excellent quenching efficiency of 2D Cu-MOF nanosheets.The presence of ATP was capable to dissociate the FAM-aptamer from Cu-MOF nanosheets and resulted in an intense fluorescence signal.The Cu-MOFs/aptamer nanoprobe enables highly sensitive and selective measurement of ATP level ranging from 10μM to 800μM with a detection limit of 4.24μM.This nanosystem also further realized in situ detection of the undulation of ATP trigged by drug stimulation,depending on the selective delivery of the nanoprobe and attractive capability of resisting nonspecific displacement.The constructed nanoprobe may supply a potential platform in clinical diagnostics and biological studies.展开更多
Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and i...Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites.PLL-based polymers and copolymers,till date,have been extensively explored in the contexts such as antibacterial agents,gene/drug/protein delivery systems,bio-sensing,bio-imaging,and tissue engineering.This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade.The review first describes the synthesis of PLL and its derivatives,followed by the main text of their recent biomedical applications and translational studies.Finally,the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed.展开更多
A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and...A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and up to 700-fold enhancement in two-photon photoluminescence.Huge enhancement in two-photon photoluminescence allows achieving a detection limit of as low as 0.0062 mg/kg,which is better than the conventional colorimetric methods.This two-photon sensing scheme has a broad dynamic range and displays excellent selectivity in detection of cartap against over other ten kinds of commonly used insecticides.展开更多
We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription ti...We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription times previously reported.This was achieved using a new dopant material,diphenyl disulphide,which was found to enable a fast,positive refractive index change using a low ultraviolet dose.These changes were investigated and found to arise from photodissociation of the diphenyl disulphide molecule and subsequent molecular reorganization.We demonstrate that gratings inscribed in these fibres can exhibit at least a 15 times higher sensitivity than silica glass fibre,despite their quick inscription times.As a demonstration of the sensitivity,we selected a highly stringent situation,namely,the monitoring of a human heartbeat and respiratory functions.These findings could permit the inscription of fibre Bragg gratings during the fibre drawing process for mass production,allowing costeffective,single-use,in vivo sensors among other potential uses.展开更多
基金supported in part by the National Natural Science Foundation of China(21974125 and 21605038)the National 111 Project of China(D20003)the Collaborative Innovation Project of Zhengzhou(Zhengzhou University)(Grant No.18XTZX12002)
文摘Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation.Herein,by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer,we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP.This nanoprobe reveals a very low fluorescence intensity because of the excellent quenching efficiency of 2D Cu-MOF nanosheets.The presence of ATP was capable to dissociate the FAM-aptamer from Cu-MOF nanosheets and resulted in an intense fluorescence signal.The Cu-MOFs/aptamer nanoprobe enables highly sensitive and selective measurement of ATP level ranging from 10μM to 800μM with a detection limit of 4.24μM.This nanosystem also further realized in situ detection of the undulation of ATP trigged by drug stimulation,depending on the selective delivery of the nanoprobe and attractive capability of resisting nonspecific displacement.The constructed nanoprobe may supply a potential platform in clinical diagnostics and biological studies.
基金This work was financially supported by the National Natural Science Foundation of China,China(No.81803467)2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant,Hong Kong(2020LKSFG18B,2020LKSFG02E)the grant for Key Disciplinary Project of Clinical Medicine under the Guangdong High-Level University Development Program,Guangdong,China(002-18120314,002-18120311).
文摘Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites.PLL-based polymers and copolymers,till date,have been extensively explored in the contexts such as antibacterial agents,gene/drug/protein delivery systems,bio-sensing,bio-imaging,and tissue engineering.This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade.The review first describes the synthesis of PLL and its derivatives,followed by the main text of their recent biomedical applications and translational studies.Finally,the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed.
基金the Ministry of Education, Singapore (R-143-000-607-112)the National Research Foundation, Prime Minister’s Office, Singapore under its Competitive Research Program (NRF-CRP10-2012-04)Medium Sized Centre Programme
文摘A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and up to 700-fold enhancement in two-photon photoluminescence.Huge enhancement in two-photon photoluminescence allows achieving a detection limit of as low as 0.0062 mg/kg,which is better than the conventional colorimetric methods.This two-photon sensing scheme has a broad dynamic range and displays excellent selectivity in detection of cartap against over other ten kinds of commonly used insecticides.
基金supported by the General Research Fund Project(n1 PolyU 152207/15E)PolyU Central Grant project 1-ZVGBsupport from the Hong Kong Research Grants Council through the Hong Kong PhD Fellowship Scheme.
文摘We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription times previously reported.This was achieved using a new dopant material,diphenyl disulphide,which was found to enable a fast,positive refractive index change using a low ultraviolet dose.These changes were investigated and found to arise from photodissociation of the diphenyl disulphide molecule and subsequent molecular reorganization.We demonstrate that gratings inscribed in these fibres can exhibit at least a 15 times higher sensitivity than silica glass fibre,despite their quick inscription times.As a demonstration of the sensitivity,we selected a highly stringent situation,namely,the monitoring of a human heartbeat and respiratory functions.These findings could permit the inscription of fibre Bragg gratings during the fibre drawing process for mass production,allowing costeffective,single-use,in vivo sensors among other potential uses.
