[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc ...[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc × Landrace × Yorkshire)with the body weight of(30.5±0.8)kg were randomly divided into five groups,three replicates each group,six pigs each replicate.The padding of five groups were:50%rice husk+50%sawdust(control group),100%spent mushroom substrate of Pleurotus eryngii(100%spent mushroom substrate group),15%rice husk+15%sawdust+70%spent mushroom substrate(70%spent mushroom substrate group),25%rice husk+25%sawdust+50%spent mushroom substrate(50%spent mushroom substrate group),35%rice husk+35%sawdust+30%spent mushroom substrate(30%spent mushroom substrate group).The pre-trial lasted 7 d,and the formal trial lasted 90 d.[Result]Compared with the control group,the number of Escherichia col in 100%,70%and 50%spent mushroom substrate groups decreased by 35.4%,24.0%and 20.8%,respectively(P<0.05);the number of salmonella successively decreased by 29.4%,19.3%and 15.2%,respectively(P<0.05);the number of lactobacillus successively increased by 21.7%,15.9%and 12.2%,respectively(P<0.05);and the number of bifidobacteria successively increased by 20.2%,16.7%and 11.8%,respectively(P<0.05).Compared with the control group,the total serum protein content in 100%,70%,50%and 30%spent mushroom substrate groups increased by 30.6%,33.0%,28.0%and 18.4%,respectively(P<0.05);and the serum urea nitrogen content decreased by 32.4%,34.5%,33.9%and 22.0%,respectively(P<0.05).There was no significant difference in serum albumin,aspartate aminotransferase(AST)and alanine aminotransferase(ALT)between treatment groups and control group.The IgA,IgG,IgM and IL-6 levels in serum of each treatment group were significantly different from those of the control group(P<0.05).There was no significant difference in IgA,IgG,IgM and IL-6 levels among 100%,70%and 50%spent mushroom substrate groups.[Conclusion]It improves the balance of intestinal microecological flora of growing-finishing pigs and enhances the immune performance of pigs by using spent mushroom substrate of P.eryngii as the fermentation bed padding.15%rice husk+15%sawdust+70%spent mushroom substrate is the optimal proportion of spent mushroom substrate for growing-finishing pigs.展开更多
To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentrati...To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentration of which is 250mg / m 3 while another group of mice are exposed to room air only served as control.The blood , alveolar lavaged fluid and lungs of the mice are collected and examined after exposed for 7 , 14and 28d , respectively.The serum analysis shows that glucose ( Glu ) values with 7dexposure , alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities with 28dinhalation , and total bile acid ( TBA ) values with 7 , 28d as well as the creatine kinase ( CK ) levels with 28dexposure are significantly different from those of controls ( P< 0.05 ) .The pathological findings exhibit that more TiO 2 nanotubes are found in the interstitium of pulmonary alveoli with the experimental time prolonged.The results suggest that TiO 2 nanotubes do produce adverse responses to the lung and serum biochemical indexes of mice.Moreover , the responses become severer with the exposed-time prolonged.展开更多
To investigate the seasonal changes in physiological and biochemical indexes of Tibetan sheep in Hongyuan area,we measured the physiological and biochemical indexes of Tibetan sheep at four different seasons. The resu...To investigate the seasonal changes in physiological and biochemical indexes of Tibetan sheep in Hongyuan area,we measured the physiological and biochemical indexes of Tibetan sheep at four different seasons. The results showed that the body temperature,respiration and heart rate of Tibetan sheep at four seasons were 39. 18- 39. 60 ℃,27. 20- 49. 04 times / min,91. 87- 106. 25 times / min,respectively. The indexes of WBC,MCV,MCH and MCHC in autumn and winter were significantly higher than those in spring and summer( P < 0. 01),while the indexes of RBC,HCT and RDW-CV in spring and summer were significantly higher than those in autumn and winter( P < 0. 01). The PLT did not vary greatly in spring,summer or autumn,but were all extremely higher than that in winter( P< 0. 01). The HGB maintained stable in the four seasons. The indexes of TP,ALB and GLO in summer and autumn were extremely higher than those in winter and spring( P < 0. 01),while the indexes of ALP and PCHE in summer and autumn were extremely lower than those in winter and spring( P < 0. 01). The indexes of AST,ALT,LDH,GLU,CHOL and CA maintained stable in all seasons.展开更多
The researches on the correlation between TCM syndromes of coronary heart disease and blood biochemical indexes from Jan.2011 to Jul.2020 were reviewed,in order to explore the association of different TCM syndromes of...The researches on the correlation between TCM syndromes of coronary heart disease and blood biochemical indexes from Jan.2011 to Jul.2020 were reviewed,in order to explore the association of different TCM syndromes of coronary heart disease with blood biochemical indexes,such as blood lipid metabolism,inflammatory factors,coagulation-fibrinolysis system,blood rheology and vascular endothelial function,which was hoped to provide a guidance on the clinical combined application of TCM syndrome differentiation and biochemical indexes of coronary heart disease,so as to promote the objectification and standardization of TCM syndrome differentiation.展开更多
BACKGROUND: At present, it is believed that the important causes of cerebral infarction are the disorders of lipid metabolism and endothelial function, and the outcomes of clinical treatment can be improved by regulat...BACKGROUND: At present, it is believed that the important causes of cerebral infarction are the disorders of lipid metabolism and endothelial function, and the outcomes of clinical treatment can be improved by regulating serum lipids and antiinflammation, etc. OBJECTIVE: To observe the effect of simvastatin, inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, on the levels of serum lipids, serum enzymic indexes and inflammatory metabolic indexes in patients with cerebral infarction. DESIGN: A comparative observation. SETTING: Department of Geriatrics, Longquanshan Hospital of Liuzhou City. PARTICIPANTS: Forty-eight patients with acute cerebral infarction were selected from the Department of Geriatrics of Longquanshan Hospital of Liuzhou from March 2004 to February 2006, including 24 males and 24 females, the mean age was (54±12) years, average disease course was (10.0±4.5) days. They were all accorded with the diagnostic standard for cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1999, and cerebral hemorrhage was excluded by cranial CT scanning. The 48 patients were randomly divided into control group (n =24) and treatment group (n =24). Informed consents were obtained from all the participants. METHODS: ① All the patients were treated according to the symptoms, besides those in the treatment group were given simvastatin (Harbin Pharm. Group Sanjing Pharmaceutical Shareholding, Co.,Ltd., No. H20010454; Batch number: 20040218; 5 mg/tablet). The initial dosage was 10 mg per day for 4 weeks, and then increased to 30 mg per day for another 4 weeks. ② Before treatment and within 1 week after treatment, the total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), creatine kinase and C reactive protein in serum were determined with Beckman-cx7 automatic biochemical analytical apparatus in both groups. ③ The differences of intergroup and intragroup data were compared with the independent-samples t test and paired samples t test. MAIN OUTCOME MEASURES: Changes of total cholesterol, triglyceride, HDL-C, LDL-C, AST, creatine kinase and C reactive protein before and treatment in both groups. RESULTS: All the 48 patients with cerebral infarction were involved in the analysis of results. ① Changes of serum lipids: The levels of serum lipids were close between the two groups before treatment (P > 0.05). After treatment, the HDL-C level in the treatment group was obviously higher than that in the control group and that before treatment [(1.34±0.12), (0.92±0.33), (0.93±0.21) mmol/L, t =7.922, 11.699, P < 0.01], and the levels of total cholesterol, triglyceride and LDL-C were obviously lower than those in the control group and those before treatment (t =2.780-7.591, P < 0.01). ② Changes of serum enzymic indexes and C reactive protein in serum: The levels of enzymes and C reactive protein in serum had no obvious differences between the two groups before treatment (P > 0.05). The levels of AST, creatine kinase and C reactive protein in serum after treatment were obviously lower than those before treatment in both groups (t =7.259-17.996, P < 0.01). The levels of levels of creatine kinase and C reactive protein in serum after treatment in the treatment group were obviously lower than those in the control group [(3.061±0.522) μkat/L, (4.6±3.1) mg/L; (4.348±0.580) μkat/L, (12.3±4.8) mg/L, t =7.910, 6.463, P < 0.01]. CONCLUSION: Compared with common treatment according to symptoms, the additional administration of simvastatin is better for improving the serum lipids, serum enzymic indexes of patients with cerebral infarction at acute period, and benefit for repairing their inflammatory damages.展开更多
This experiment was conducted to study the effects of dietary alfalfa flavonoids extraction supplemental level on growth performance, organ development and blood biochemical indexes of Yangzhou geese at the age of 28 ...This experiment was conducted to study the effects of dietary alfalfa flavonoids extraction supplemental level on growth performance, organ development and blood biochemical indexes of Yangzhou geese at the age of 28 to 70 days. Two hundred and forty 21-day-old healthy male geese with similar body weight were randomly distributed into 4 groups with 6 replicates per group and 10 geese per replicate. Geese in the control group were fed a basal diet and the others in the experimental groups(groups 1,2, and 3) were fed experimental diets supplemented with 150, 300 and 450 mg/kg alfalfa flavonoids extraction(the concentration of it was 81%), respectively. The experiment had 7 days for pre-test and 42 days for formal test.The results showed that the final body weight and average daily intake of group 2 were significantly higher than those of other groups(P < 0.05). The average daily gain of group 2 was significantly higher than that in the control group and group 1(P < 0.05). There was no significant difference in feed-to-gain ratio between each group(P > 0.05). Pre-slaughter live weight, carcass weight, slaughter rate, semieviscerated weight, semi-eviscerated rate, eviscerated weight, eviscerated rate, leg muscle weight and leg muscle rate had no significant difference between each group(P > 0.05). The breast muscle weight and ratio of each test group were significantly higher than those in the control group(P < 0.05) and the group2 was the best. The abdominal fat weight and ratio in the group 1 were significantly higher than those in the control group and group 3(P < 0.05) and the tibia weight in the group 2 was significantly higher than that in the control group and group 1(P < 0.05); There were no significant differences in heart weight,liver weight and the gland stomach weight among all groups(P > 0.05). Spleen weight in test groups was significantly higher than that in the control group(P < 0.05). The bursa weight and muscular stomach weight in the group 2 were significantly higher than those in the control group and group 1(P < 0.05). In serum, total cholesterol, triglycerides, low-density lipoprotein and urea nitrogen in the group 2 were significantly lower comparing with those in the control group(P < 0.05). High-density lipoprotein in the group 2 was significantly higher than that in other groups(P < 0.05). There were no significant differences in total serum protein, albumin, globulin and albumin/globulin among all groups(P > 0.05). Alanine aminotransferase and aspartate transaminase(AST) in groups 2 and 3 were higher than those in the group1 and control group but not obvious(P > 0.05) and alkaline phosphatase(ALP) in groups 1 and 2 was higher than that in the control group and group 3(P > 0.05). It is concluded that alfalfa flavonoids extraction added in dietary feed improve the growth performance, organ development and blood biochemical indexes of Yangzhou geese. It is concluded that 300 mg/kg supplemental level of the dietary alfalfa flavonoids extraction is optimal in this experiment.展开更多
Objective:To investigate whether the specific traditional Chinese medicine(TCM)constitution of individuals can be defined by certain biological indexes instead of answering the questionnaire,and to explore the possibi...Objective:To investigate whether the specific traditional Chinese medicine(TCM)constitution of individuals can be defined by certain biological indexes instead of answering the questionnaire,and to explore the possibility of discriminating nine TCM constitutions from each other simultaneously using biological indexes.Methods:Blood and urine samples from 152 individuals with nine TCM constitutions were collected,and the related biological indexes were analyzed combining ANOVA,multiple comparison,discriminant analysis,and support vector machine.Results:We found that 4 out of 24 blood routine indexes,7 out of 10 urine routine indexes,and 12 out of 32 biochemical indexes showed differences among the constitutions.High-sensitivity C-reactive protein,apolipoprotein A1,and alkaline phosphatase were potential candidates for screening out individuals with unbalanced constitutions.Combining uric acid,high-density lipoprotein,apolipoprotein A1,creatine kinase,total protein,aspartate aminotransferase,total bile acid,dehydrogenase,sodium,and calcium levels had the potential to directly distinguish the nine TCM constitutions from each other.Among these indexes,the highest ratio of discriminant analysis between two constitutions was 95.5%,while the lowest was 66.1%.Conclusion:Our results suggest that some biochemical and urine indexes are related to various TCM constitutions,and thus they have the potential to be used for TCM constitution classification.展开更多
More than 90% of HIV-positive children come from mother-to-child transmission, so mother-to-child interdiction is the main measure to prevent AIDS in children. Relevant data show that the incidence of mother-to-child ...More than 90% of HIV-positive children come from mother-to-child transmission, so mother-to-child interdiction is the main measure to prevent AIDS in children. Relevant data show that the incidence of mother-to-child transmission of HIV usually fluctuates between 15% and 50% without intervention. Standardized and effective comprehensive intervention can reduce the transmission rate of mother to child to less than 1%. At present, it is believed that mother-to-child transmission of AIDS can be blocked by comprehensive intervention, and its mechanism has been clearly studied. Combined with highly effective antiviral treatment, safe labor and artificial feeding, the mother to child transmission rate can be reduced to less than 1%. However, due to the effect of drugs on mother-to-child transmission of AIDS may lead to some changes in the main biochemical indicators of mother-to-child, there is no systematic analysis of the viral load, T lymphocyte subsets and major biochemical indicators of HIV/AIDS pregnant women before and after maternal-to-child transmission. In this study, the viral load, T lymphocyte subsets and major biochemical indicators of HIV/AIDS pregnant women before and after maternal-infant blockade were dynamically analyzed. It is hoped that this study will help to observe the basic physical fitness and disease development of pregnant women with HIV/AIDS during pregnancy, to provide a strong basis for the treatment and evaluation of maternal-infant blockade of HIV/AIDS pregnant women, and to establish a complete set of laboratory indicators to understand the body status of pregnant women at all stages to minimize the probability of fetal transmission, which will make good economic and social benefits.展开更多
BACKGROUND Mucinous cystic neoplasm(MCN)of the pancreas is characterized by mucinproducing columnar epithelium and dense ovarian-type stroma and at risk for malignant transformation.Early diagnosis and treatment of MC...BACKGROUND Mucinous cystic neoplasm(MCN)of the pancreas is characterized by mucinproducing columnar epithelium and dense ovarian-type stroma and at risk for malignant transformation.Early diagnosis and treatment of MCN are particularly important.AIM To investigate the clinical characteristics of and management strategies for pancreatic mucinous cystadenoma(MCA)and mucinous cystadenocarcinoma(MCC).METHODS The clinical and pathological data of 82 patients with pancreatic MCA and MCC who underwent surgical resection at our department between April 2015 and March 2019 were retrospectively analyzed.RESULTS Of the 82 patients included in this study,70 had MCA and 12 had MCC.Tumor size of MCC was larger than that of MCA(P=0.049).Age and serum levels of tumor markers carcinoembryonic antigen(CEA),carbohydrate antigen(CA)19-9,and CA12-5 were significantly higher in MCC than in MCA patients(P=0.005,0.026,and 0.037,respectively).MCA tumor size was positively correlated with serum CA19-9 levels(r=0.389,P=0.001).Compared with MCC,MCA had a higher minimally invasive surgery rate(P=0.014).In the MCA group,the rate of major complications was 5.7%and that of clinically relevant pancreatic fistula was 8.6%;the corresponding rates in the MCC group were 16.7%and 16.7%,respectively.CONCLUSION Tumor size,age,and serum CEA,CA19-9,and CA12-5 levels may contribute to management of patients with MCN.Surgical resection is the primary treatment modality for MCC and MCA.展开更多
Objective:To analyze the clinical characteristics and chest CT imaging characteristics of patients with confirmed COVID-19(COVID-19)and patients with suspected COVID-19.Methods:The study time span was from February 20...Objective:To analyze the clinical characteristics and chest CT imaging characteristics of patients with confirmed COVID-19(COVID-19)and patients with suspected COVID-19.Methods:The study time span was from February 2020 to May 2020.The case samples were selected from 72 patients with confirmed covid-19 and suspected covid-19 diagnosed and treated by The First People’s Hospital of Yinchuan and Yinchuan Temporary Emergency Hospital,including 38 patients with confirmed covid-19 and 34 patients with suspected covid-19.All patients underwent laboratory examination and chest CT examination,and the specific examination results were compared and analyzed.Results:There were significant differences in number of white blood cell,percentage of lymphocytes,creatine kinase and erythrocyte sedimentation rate between confirmed and suspected COVID-19 patients(P<0.05).The CT imaging characteristics of COVID-19 patients were compared with those of suspected COVID-19 patients.The lesions of COVID-19 patients were mostly characterized by mixed ground glass density and pure ground glass density.There were vascular thickening and interstitial thickness increase,and accompanied by bronchiectasis or air bronchogram.The distribution of lesions was mostly subpleural without pleural effusion.The lesion area of suspected COVID-19 patients mostly showed solid density and mixed ground glass density.The lesion was distributed along bronchovascular and pleural effusion was observed.Conclusion:There are some differences in biochemical indexes and chest CT images between confirmed and suspected covid-19 patients,which can be used for differential diagnosis.展开更多
Melatonin(MLT)is a hormonal substance found in many organisms and can improve plant stress resistance.In this study,the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under differ...Melatonin(MLT)is a hormonal substance found in many organisms and can improve plant stress resistance.In this study,the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl2 at the two-leaf stage.The growth,physiological and biochemical responses of the seedlings and the expression of cadmium(Cd)-related genes under exogenous melatonin(MLT)treatment were assessed.The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species(ROS)production and removal,resulting in ROS accumulation,membrane lipid peroxidation,and impaired growth and development.Following the application of exogenous MLT to rice seedlings,increases in plant biomass including both underground and above-ground areas were observed.MLT also scavenged the inhibition of superoxide dismutase(SOD)and peroxidase(POD)in a concentration dependent manner in response to Cd stress.Catalase(CAT)activity and malondialdehyde(MDA)expression also decreased following MLT treatment.Amongst the six Cd-related genes assessed,five genes were downregulated and one was up-regulated in response to MLT treatment.Taken together,these data demonstrate that MLT improves the resilience of rice seedlings at the biochemical,physiological,and molecular levels,and diminishes the damage caused by Cd stress.展开更多
Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four...Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four flying saucer athletes in Zhejiang province were selected and randomly divided into training group and control group,15 in each group.Peripheral venous blood of each group was taken before and after the experiment.The HSP72 content of lymphocyte,blood routine and biochemical indexes were measured respectively.Results:There was significant difference in white blood cell in training group count before and after the experiment(P<0.05).The expression of HSP72 and the white blood cell in training group was higher than that control group(P<0.05).Conclusions:Organism could be affected and turned to stress state due to training under high temperature.It is necessary to provide the safeguard for the flying saucer athletes under high temperature in order to prevent the damage.展开更多
基金Supported by Animal Medicine Specialized Group Program of Beijing Characteristic High Level Backbone Specialized Group Project(PXM2020-157102-000060)General Project of Science and Technology Plan of Beijing Municipal Education Commission(KM201512448004)+1 种基金Scientific and Technological Capacity Enhancement Project of"Vegetable Basket"New Production and Business Entity of Beijing Municipal Agricultural Commission(20150203-5)Doctoral Fund of Beijing Vocational College of Agriculture(XY-BS-15-01).
文摘[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc × Landrace × Yorkshire)with the body weight of(30.5±0.8)kg were randomly divided into five groups,three replicates each group,six pigs each replicate.The padding of five groups were:50%rice husk+50%sawdust(control group),100%spent mushroom substrate of Pleurotus eryngii(100%spent mushroom substrate group),15%rice husk+15%sawdust+70%spent mushroom substrate(70%spent mushroom substrate group),25%rice husk+25%sawdust+50%spent mushroom substrate(50%spent mushroom substrate group),35%rice husk+35%sawdust+30%spent mushroom substrate(30%spent mushroom substrate group).The pre-trial lasted 7 d,and the formal trial lasted 90 d.[Result]Compared with the control group,the number of Escherichia col in 100%,70%and 50%spent mushroom substrate groups decreased by 35.4%,24.0%and 20.8%,respectively(P<0.05);the number of salmonella successively decreased by 29.4%,19.3%and 15.2%,respectively(P<0.05);the number of lactobacillus successively increased by 21.7%,15.9%and 12.2%,respectively(P<0.05);and the number of bifidobacteria successively increased by 20.2%,16.7%and 11.8%,respectively(P<0.05).Compared with the control group,the total serum protein content in 100%,70%,50%and 30%spent mushroom substrate groups increased by 30.6%,33.0%,28.0%and 18.4%,respectively(P<0.05);and the serum urea nitrogen content decreased by 32.4%,34.5%,33.9%and 22.0%,respectively(P<0.05).There was no significant difference in serum albumin,aspartate aminotransferase(AST)and alanine aminotransferase(ALT)between treatment groups and control group.The IgA,IgG,IgM and IL-6 levels in serum of each treatment group were significantly different from those of the control group(P<0.05).There was no significant difference in IgA,IgG,IgM and IL-6 levels among 100%,70%and 50%spent mushroom substrate groups.[Conclusion]It improves the balance of intestinal microecological flora of growing-finishing pigs and enhances the immune performance of pigs by using spent mushroom substrate of P.eryngii as the fermentation bed padding.15%rice husk+15%sawdust+70%spent mushroom substrate is the optimal proportion of spent mushroom substrate for growing-finishing pigs.
基金Supported by the Ford University Research Projectthe "Peak of Six Major Human Resources Plan" of Jiangsu Province
文摘To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentration of which is 250mg / m 3 while another group of mice are exposed to room air only served as control.The blood , alveolar lavaged fluid and lungs of the mice are collected and examined after exposed for 7 , 14and 28d , respectively.The serum analysis shows that glucose ( Glu ) values with 7dexposure , alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities with 28dinhalation , and total bile acid ( TBA ) values with 7 , 28d as well as the creatine kinase ( CK ) levels with 28dexposure are significantly different from those of controls ( P< 0.05 ) .The pathological findings exhibit that more TiO 2 nanotubes are found in the interstitium of pulmonary alveoli with the experimental time prolonged.The results suggest that TiO 2 nanotubes do produce adverse responses to the lung and serum biochemical indexes of mice.Moreover , the responses become severer with the exposed-time prolonged.
基金Supported by Achievement Transformation Project of Science & Technology Department of Sichuan Province "Hybridization Improvement and Demonstration Promotion of Tibetan Sheep with White Suffolk Sheep"
文摘To investigate the seasonal changes in physiological and biochemical indexes of Tibetan sheep in Hongyuan area,we measured the physiological and biochemical indexes of Tibetan sheep at four different seasons. The results showed that the body temperature,respiration and heart rate of Tibetan sheep at four seasons were 39. 18- 39. 60 ℃,27. 20- 49. 04 times / min,91. 87- 106. 25 times / min,respectively. The indexes of WBC,MCV,MCH and MCHC in autumn and winter were significantly higher than those in spring and summer( P < 0. 01),while the indexes of RBC,HCT and RDW-CV in spring and summer were significantly higher than those in autumn and winter( P < 0. 01). The PLT did not vary greatly in spring,summer or autumn,but were all extremely higher than that in winter( P< 0. 01). The HGB maintained stable in the four seasons. The indexes of TP,ALB and GLO in summer and autumn were extremely higher than those in winter and spring( P < 0. 01),while the indexes of ALP and PCHE in summer and autumn were extremely lower than those in winter and spring( P < 0. 01). The indexes of AST,ALT,LDH,GLU,CHOL and CA maintained stable in all seasons.
基金General Project of National Natural Science Foundation of China(No.81774260)。
文摘The researches on the correlation between TCM syndromes of coronary heart disease and blood biochemical indexes from Jan.2011 to Jul.2020 were reviewed,in order to explore the association of different TCM syndromes of coronary heart disease with blood biochemical indexes,such as blood lipid metabolism,inflammatory factors,coagulation-fibrinolysis system,blood rheology and vascular endothelial function,which was hoped to provide a guidance on the clinical combined application of TCM syndrome differentiation and biochemical indexes of coronary heart disease,so as to promote the objectification and standardization of TCM syndrome differentiation.
文摘BACKGROUND: At present, it is believed that the important causes of cerebral infarction are the disorders of lipid metabolism and endothelial function, and the outcomes of clinical treatment can be improved by regulating serum lipids and antiinflammation, etc. OBJECTIVE: To observe the effect of simvastatin, inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, on the levels of serum lipids, serum enzymic indexes and inflammatory metabolic indexes in patients with cerebral infarction. DESIGN: A comparative observation. SETTING: Department of Geriatrics, Longquanshan Hospital of Liuzhou City. PARTICIPANTS: Forty-eight patients with acute cerebral infarction were selected from the Department of Geriatrics of Longquanshan Hospital of Liuzhou from March 2004 to February 2006, including 24 males and 24 females, the mean age was (54±12) years, average disease course was (10.0±4.5) days. They were all accorded with the diagnostic standard for cerebral infarction set by the Fourth National Academic Meeting for Cerebrovascular Disease in 1999, and cerebral hemorrhage was excluded by cranial CT scanning. The 48 patients were randomly divided into control group (n =24) and treatment group (n =24). Informed consents were obtained from all the participants. METHODS: ① All the patients were treated according to the symptoms, besides those in the treatment group were given simvastatin (Harbin Pharm. Group Sanjing Pharmaceutical Shareholding, Co.,Ltd., No. H20010454; Batch number: 20040218; 5 mg/tablet). The initial dosage was 10 mg per day for 4 weeks, and then increased to 30 mg per day for another 4 weeks. ② Before treatment and within 1 week after treatment, the total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), creatine kinase and C reactive protein in serum were determined with Beckman-cx7 automatic biochemical analytical apparatus in both groups. ③ The differences of intergroup and intragroup data were compared with the independent-samples t test and paired samples t test. MAIN OUTCOME MEASURES: Changes of total cholesterol, triglyceride, HDL-C, LDL-C, AST, creatine kinase and C reactive protein before and treatment in both groups. RESULTS: All the 48 patients with cerebral infarction were involved in the analysis of results. ① Changes of serum lipids: The levels of serum lipids were close between the two groups before treatment (P > 0.05). After treatment, the HDL-C level in the treatment group was obviously higher than that in the control group and that before treatment [(1.34±0.12), (0.92±0.33), (0.93±0.21) mmol/L, t =7.922, 11.699, P < 0.01], and the levels of total cholesterol, triglyceride and LDL-C were obviously lower than those in the control group and those before treatment (t =2.780-7.591, P < 0.01). ② Changes of serum enzymic indexes and C reactive protein in serum: The levels of enzymes and C reactive protein in serum had no obvious differences between the two groups before treatment (P > 0.05). The levels of AST, creatine kinase and C reactive protein in serum after treatment were obviously lower than those before treatment in both groups (t =7.259-17.996, P < 0.01). The levels of levels of creatine kinase and C reactive protein in serum after treatment in the treatment group were obviously lower than those in the control group [(3.061±0.522) μkat/L, (4.6±3.1) mg/L; (4.348±0.580) μkat/L, (12.3±4.8) mg/L, t =7.910, 6.463, P < 0.01]. CONCLUSION: Compared with common treatment according to symptoms, the additional administration of simvastatin is better for improving the serum lipids, serum enzymic indexes of patients with cerebral infarction at acute period, and benefit for repairing their inflammatory damages.
文摘This experiment was conducted to study the effects of dietary alfalfa flavonoids extraction supplemental level on growth performance, organ development and blood biochemical indexes of Yangzhou geese at the age of 28 to 70 days. Two hundred and forty 21-day-old healthy male geese with similar body weight were randomly distributed into 4 groups with 6 replicates per group and 10 geese per replicate. Geese in the control group were fed a basal diet and the others in the experimental groups(groups 1,2, and 3) were fed experimental diets supplemented with 150, 300 and 450 mg/kg alfalfa flavonoids extraction(the concentration of it was 81%), respectively. The experiment had 7 days for pre-test and 42 days for formal test.The results showed that the final body weight and average daily intake of group 2 were significantly higher than those of other groups(P < 0.05). The average daily gain of group 2 was significantly higher than that in the control group and group 1(P < 0.05). There was no significant difference in feed-to-gain ratio between each group(P > 0.05). Pre-slaughter live weight, carcass weight, slaughter rate, semieviscerated weight, semi-eviscerated rate, eviscerated weight, eviscerated rate, leg muscle weight and leg muscle rate had no significant difference between each group(P > 0.05). The breast muscle weight and ratio of each test group were significantly higher than those in the control group(P < 0.05) and the group2 was the best. The abdominal fat weight and ratio in the group 1 were significantly higher than those in the control group and group 3(P < 0.05) and the tibia weight in the group 2 was significantly higher than that in the control group and group 1(P < 0.05); There were no significant differences in heart weight,liver weight and the gland stomach weight among all groups(P > 0.05). Spleen weight in test groups was significantly higher than that in the control group(P < 0.05). The bursa weight and muscular stomach weight in the group 2 were significantly higher than those in the control group and group 1(P < 0.05). In serum, total cholesterol, triglycerides, low-density lipoprotein and urea nitrogen in the group 2 were significantly lower comparing with those in the control group(P < 0.05). High-density lipoprotein in the group 2 was significantly higher than that in other groups(P < 0.05). There were no significant differences in total serum protein, albumin, globulin and albumin/globulin among all groups(P > 0.05). Alanine aminotransferase and aspartate transaminase(AST) in groups 2 and 3 were higher than those in the group1 and control group but not obvious(P > 0.05) and alkaline phosphatase(ALP) in groups 1 and 2 was higher than that in the control group and group 3(P > 0.05). It is concluded that alfalfa flavonoids extraction added in dietary feed improve the growth performance, organ development and blood biochemical indexes of Yangzhou geese. It is concluded that 300 mg/kg supplemental level of the dietary alfalfa flavonoids extraction is optimal in this experiment.
基金supported by the National Key Research and Development Project (2019YFC1710104)the National Natural Science Foundation of China (81430099)+1 种基金the International Cooperation and Exchanges (2014DFA32950)the Fundamental Research Funds for the Central Universities (2020-JYB-XJSJJ-026)
文摘Objective:To investigate whether the specific traditional Chinese medicine(TCM)constitution of individuals can be defined by certain biological indexes instead of answering the questionnaire,and to explore the possibility of discriminating nine TCM constitutions from each other simultaneously using biological indexes.Methods:Blood and urine samples from 152 individuals with nine TCM constitutions were collected,and the related biological indexes were analyzed combining ANOVA,multiple comparison,discriminant analysis,and support vector machine.Results:We found that 4 out of 24 blood routine indexes,7 out of 10 urine routine indexes,and 12 out of 32 biochemical indexes showed differences among the constitutions.High-sensitivity C-reactive protein,apolipoprotein A1,and alkaline phosphatase were potential candidates for screening out individuals with unbalanced constitutions.Combining uric acid,high-density lipoprotein,apolipoprotein A1,creatine kinase,total protein,aspartate aminotransferase,total bile acid,dehydrogenase,sodium,and calcium levels had the potential to directly distinguish the nine TCM constitutions from each other.Among these indexes,the highest ratio of discriminant analysis between two constitutions was 95.5%,while the lowest was 66.1%.Conclusion:Our results suggest that some biochemical and urine indexes are related to various TCM constitutions,and thus they have the potential to be used for TCM constitution classification.
文摘More than 90% of HIV-positive children come from mother-to-child transmission, so mother-to-child interdiction is the main measure to prevent AIDS in children. Relevant data show that the incidence of mother-to-child transmission of HIV usually fluctuates between 15% and 50% without intervention. Standardized and effective comprehensive intervention can reduce the transmission rate of mother to child to less than 1%. At present, it is believed that mother-to-child transmission of AIDS can be blocked by comprehensive intervention, and its mechanism has been clearly studied. Combined with highly effective antiviral treatment, safe labor and artificial feeding, the mother to child transmission rate can be reduced to less than 1%. However, due to the effect of drugs on mother-to-child transmission of AIDS may lead to some changes in the main biochemical indicators of mother-to-child, there is no systematic analysis of the viral load, T lymphocyte subsets and major biochemical indicators of HIV/AIDS pregnant women before and after maternal-to-child transmission. In this study, the viral load, T lymphocyte subsets and major biochemical indicators of HIV/AIDS pregnant women before and after maternal-infant blockade were dynamically analyzed. It is hoped that this study will help to observe the basic physical fitness and disease development of pregnant women with HIV/AIDS during pregnancy, to provide a strong basis for the treatment and evaluation of maternal-infant blockade of HIV/AIDS pregnant women, and to establish a complete set of laboratory indicators to understand the body status of pregnant women at all stages to minimize the probability of fetal transmission, which will make good economic and social benefits.
文摘BACKGROUND Mucinous cystic neoplasm(MCN)of the pancreas is characterized by mucinproducing columnar epithelium and dense ovarian-type stroma and at risk for malignant transformation.Early diagnosis and treatment of MCN are particularly important.AIM To investigate the clinical characteristics of and management strategies for pancreatic mucinous cystadenoma(MCA)and mucinous cystadenocarcinoma(MCC).METHODS The clinical and pathological data of 82 patients with pancreatic MCA and MCC who underwent surgical resection at our department between April 2015 and March 2019 were retrospectively analyzed.RESULTS Of the 82 patients included in this study,70 had MCA and 12 had MCC.Tumor size of MCC was larger than that of MCA(P=0.049).Age and serum levels of tumor markers carcinoembryonic antigen(CEA),carbohydrate antigen(CA)19-9,and CA12-5 were significantly higher in MCC than in MCA patients(P=0.005,0.026,and 0.037,respectively).MCA tumor size was positively correlated with serum CA19-9 levels(r=0.389,P=0.001).Compared with MCC,MCA had a higher minimally invasive surgery rate(P=0.014).In the MCA group,the rate of major complications was 5.7%and that of clinically relevant pancreatic fistula was 8.6%;the corresponding rates in the MCC group were 16.7%and 16.7%,respectively.CONCLUSION Tumor size,age,and serum CEA,CA19-9,and CA12-5 levels may contribute to management of patients with MCN.Surgical resection is the primary treatment modality for MCC and MCA.
基金Science and Technology Support of Key R&D Plan of Ningxia Autonomous Region“Novel Coronavirus Pneumonia Prevention and Control”special Project(Project No.2020BEG03057,2020BEG03058)。
文摘Objective:To analyze the clinical characteristics and chest CT imaging characteristics of patients with confirmed COVID-19(COVID-19)and patients with suspected COVID-19.Methods:The study time span was from February 2020 to May 2020.The case samples were selected from 72 patients with confirmed covid-19 and suspected covid-19 diagnosed and treated by The First People’s Hospital of Yinchuan and Yinchuan Temporary Emergency Hospital,including 38 patients with confirmed covid-19 and 34 patients with suspected covid-19.All patients underwent laboratory examination and chest CT examination,and the specific examination results were compared and analyzed.Results:There were significant differences in number of white blood cell,percentage of lymphocytes,creatine kinase and erythrocyte sedimentation rate between confirmed and suspected COVID-19 patients(P<0.05).The CT imaging characteristics of COVID-19 patients were compared with those of suspected COVID-19 patients.The lesions of COVID-19 patients were mostly characterized by mixed ground glass density and pure ground glass density.There were vascular thickening and interstitial thickness increase,and accompanied by bronchiectasis or air bronchogram.The distribution of lesions was mostly subpleural without pleural effusion.The lesion area of suspected COVID-19 patients mostly showed solid density and mixed ground glass density.The lesion was distributed along bronchovascular and pleural effusion was observed.Conclusion:There are some differences in biochemical indexes and chest CT images between confirmed and suspected covid-19 patients,which can be used for differential diagnosis.
基金supported by Hangzhou Scientific and Technological Program(20170432B03)the National Key Technology Research and Development Program(2015BAD01B02)the National Natural Science Foundation of China(31661143006).
文摘Melatonin(MLT)is a hormonal substance found in many organisms and can improve plant stress resistance.In this study,the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl2 at the two-leaf stage.The growth,physiological and biochemical responses of the seedlings and the expression of cadmium(Cd)-related genes under exogenous melatonin(MLT)treatment were assessed.The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species(ROS)production and removal,resulting in ROS accumulation,membrane lipid peroxidation,and impaired growth and development.Following the application of exogenous MLT to rice seedlings,increases in plant biomass including both underground and above-ground areas were observed.MLT also scavenged the inhibition of superoxide dismutase(SOD)and peroxidase(POD)in a concentration dependent manner in response to Cd stress.Catalase(CAT)activity and malondialdehyde(MDA)expression also decreased following MLT treatment.Amongst the six Cd-related genes assessed,five genes were downregulated and one was up-regulated in response to MLT treatment.Taken together,these data demonstrate that MLT improves the resilience of rice seedlings at the biochemical,physiological,and molecular levels,and diminishes the damage caused by Cd stress.
基金Key Program of Zhejiang Sports Bureau(2017(397)-09).
文摘Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four flying saucer athletes in Zhejiang province were selected and randomly divided into training group and control group,15 in each group.Peripheral venous blood of each group was taken before and after the experiment.The HSP72 content of lymphocyte,blood routine and biochemical indexes were measured respectively.Results:There was significant difference in white blood cell in training group count before and after the experiment(P<0.05).The expression of HSP72 and the white blood cell in training group was higher than that control group(P<0.05).Conclusions:Organism could be affected and turned to stress state due to training under high temperature.It is necessary to provide the safeguard for the flying saucer athletes under high temperature in order to prevent the damage.
