Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to ...Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to the health,social well-being,and economic conditions of millions of people worldwide.Therefore,there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission.Quantum dots(QDs)are typical fluorescence nanomaterials with high quantum yield,broad absorbance range,narrow and size-dependent emission,and good stability.QDs-based nanotechnology has been found to be effective method with rapid response,easy operation,high sensitivity,and good specificity,and has been widely applied for the detection of different viruses.However,until now,no systematic and critical review has been published on this important research area.Hence,in this review,we aim to provide a comprehensive coverage of various QDs-based virus detection methods.The fundamental investigations have been reviewed,including information related to the synthesis and biofunctionalization of QDs,QDs-based viral nucleic acid detection strategies,and QDs-based immunoassays.The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.展开更多
Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）,...Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）, and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement （CPC） containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34＋ cells which were reprogrammed by a single episomal vector, iPSCs were cultured to form embryoid bodies （EBs）, and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC： RGD （Arg-Gly-Asp） peptides, fibronectin （Fn）, fibronectin-like engineered polymer protein （FEPP）, extracellular matrix Geltrex, and platelet concentrate, iPSC-MSCs were seeded on five biofunctionalized CPCs： CPC-RGD, CPC-Fn, CPC- FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and coUagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering, iPSC- MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/ orthopedic repairs.展开更多
Osteonecrosis is a common orthopedic disease in clinic,resulting in joint collapse if no appropriate treatment is performed in time.Core decompression is a general treatment modality for early osteonecrosis.However,ef...Osteonecrosis is a common orthopedic disease in clinic,resulting in joint collapse if no appropriate treatment is performed in time.Core decompression is a general treatment modality for early osteonecrosis.However,effective bone regeneration in the necrotic area is still a significant challenge.This study developed a biofunctionalized composite scaffold(PLGA/nHA30VEGF)for osteonecrosis therapy through potentiation of osteoconduction,angiogenesis,and a favorable metabolic microenvironment.The composite scaffold had a porosity of 87.7%and compressive strength of 8.9 MPa.PLGA/nHA30VEGF had an average pore size of 227.6μm and a water contact angle of 56.5◦with a sustained release profile of vascular endothelial growth factor(VEGF).After the implantation of PLGA/nHA30VEGF,various osteogenic and angiogenic biomarkers were upregulated by 2-9 fold compared with no treatment.Additionally,the metabolomic and lipidomic profiling studies demonstrated that PLGA/nHA30VEGF effectively regulated the multiple metabolites and more than 20 inordinate metabolic pathways in osteonecrosis.The excellent performances reveal that the biofunctionalized composite scaffold provides an advanced adjuvant therapy modality for osteonecrosis.展开更多
Reactive oxygen species （ROS） can be caused by mechanical, thermal, infectious, and chemical stimuli, and their negative effects on the health of humans and other animals are of considerable concern. The nuclear fac...Reactive oxygen species （ROS） can be caused by mechanical, thermal, infectious, and chemical stimuli, and their negative effects on the health of humans and other animals are of considerable concern. The nuclear factor （erythroid-derived 2）-like 2/Kelch-like ECH-associated protein 1 （Nrf2/Keap1） system plays a major role in maintaining the balance between the production and elimination of ROS via the regulation of a series of detoxifying and antioxidant enzyme gene expressions by means of the antioxidant response element （ARE）. Dietary phytochemicals, which are generally found in vegetables, fruits, grains, and herbs, have been reported to have health benefits and to improve the growth performance and meat quality of farm animals through the regulation of Nrf2-mediated phase II enzymes in a variety of ways. However, the enormous quantity of somewhat chaotic data that is available on the effects of phytochemicals needs to be properly classified according to the functions or mechanisms of phytochemicals, in this review, we first introduce the antioxidant properties of phytochemicals and their relation to the Nrf2/Keap1 system. We then summarize the effects of phytochemicals on the growth performance, meat quality, and intestinal microbiota of farm animals via targeting the Nrf2/Keap1 system. These exhaustive data contribute to better illuminate the underlying biofunctional properties of phytochemicals in farm animals.展开更多
Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and...Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and an improved prognosis.Innovative design and advanced biointerface engineering have promoted the development of various nanocarriers for optimized drug delivery.Keeping in mind the biological framework of the tumormicroenvironment,biomembrane-camouflaged nanoplatforms have been a research focus,reflecting their superiority in cancer targeting.In this review,we summarize the development of various biomimetic cell membrane-camouflaged nanoplatforms for cancertargeted drug delivery,which are classified according to the membranes fromdifferent cells.The challenges and opportunities of the advanced biointerface engineering drug delivery nanosystems in cancer therapy are discussed.展开更多
Bone marrow-derived mesenchymal stem cells(BMSCs)play a critical role in the osseointegration of bone and orthopedic implant.However,osseointegration between the Ti-based implants and the surrounding bone tissue must ...Bone marrow-derived mesenchymal stem cells(BMSCs)play a critical role in the osseointegration of bone and orthopedic implant.However,osseointegration between the Ti-based implants and the surrounding bone tissue must be improved due to titanium’s inherent defects.Surface modification stands out as a versatile technique to create instructive biomaterials that can actively direct stem cell fate.Here,we summarize the current approaches to promoting BMSC osteogenesis on the surface of titanium and its alloys.We will highlight the utilization of the unique properties of titanium and its alloys in promoting tissue regeneration,and discuss recent advances in understanding their role in regenerative medicine.We aim to provide a systematic and comprehensive review of approaches to promoting BMSC osteogenesis on the orthopedic implant surface.展开更多
Cordyceps is treasured entomopathogenic fungi that have been used as antitumor,immunomodulating,antioxidant,and pro-sexual agent.Cordyceps,also called DongChongXiaCao in Chinese,Yartsa Gunbu(Tibetan),means winter worm...Cordyceps is treasured entomopathogenic fungi that have been used as antitumor,immunomodulating,antioxidant,and pro-sexual agent.Cordyceps,also called DongChongXiaCao in Chinese,Yartsa Gunbu(Tibetan),means winter worm-summer grass.Natural Cordyceps sinensis with parasitic hosts is difficult to be collected and the recent findings on its potential pharmacological functions,resulted in skyrocketing prices.Therefore,finding a mass-production method or an alternative for C.sinensis products is a top-priority task.In this review,we describe current status of Cordyceps research and its recent developments in Taiwan.The content and pharmacological activities of four major industrial species of Cordyceps(C.sinensis,Cordyceps militaris,Cordyceps cicadae and Cordyceps sobolifera)used in Taiwan,were reviewed.Moreover,we highlighted the effect of using different methods of fermentation and production on the morphology and chemical content of Cordyceps sp.Finally,we summarized the bottle-necks and challenges facing Cordyceps research as well as we proposed future road map for Cordyceps industry in Taiwan.展开更多
In this paper diamond like carbon (DLC) /stainless steel biofunctionally gradient coatings have been prepared with two target magnetron cosputtering to solve the poor adhesion of DLC having excellent haemocompatibilit...In this paper diamond like carbon (DLC) /stainless steel biofunctionally gradient coatings have been prepared with two target magnetron cosputtering to solve the poor adhesion of DLC having excellent haemocompatibility to implant stainless steel having high strength and toughness.It has been Known from the experimental results that the adhesion of DLC coated to implant stainless steel have been significantly improved with the gradient interlayers,and biofuntionally gradient materials having excellent haemocompatibilty,strength and toughness have been prepared successively.展开更多
Mesoporous bioactive glasses(MBGs),which belong to the category of modern porous nanomaterials,have garnered significant attention due to their impressive biological activities,appealing physicochemical properties,and...Mesoporous bioactive glasses(MBGs),which belong to the category of modern porous nanomaterials,have garnered significant attention due to their impressive biological activities,appealing physicochemical properties,and desirable morphological features.They hold immense potential for utilization in diverse fields,including adsorption,separation,catalysis,bioengineering,and medicine.Despite possessing interior porous structures,excellent morphological characteristics,and superior biocompatibility,primitive MBGs face challenges related to weak encapsulation efficiency,drug loading,and mechanical strength when applied in biomedical fields.It is important to note that the advantageous attributes of MBGs can be effectively preserved by incorporating supramolecular assemblies,miscellaneous metal species,and their conjugates into the material surfaces or intrinsic mesoporous networks.The innovative advancements in these modified colloidal inorganic nanocarriers inspire researchers to explore novel applications,such as stimuli-responsive drug delivery,with exceptional in-vivo performances.In view of the above,we outline the fabrication process of calcium-silicon-phosphorus based MBGs,followed by discussions on their significant progress in various engineered strategies involving surface functionalization,nanostructures,and network modification.Furthermore,we emphasize the recent advancements in the textural and physicochemical properties of MBGs,along with their theranostic potentials in multiple cancerous and non-cancerous diseases.Lastly,we recapitulate compelling viewpoints,with specific considerations given from bench to bedside.展开更多
Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of m...Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of metallic implants for high-performance biofunctions.Calcium phosphates(CaPs)are the major inorganic component of bone tissues,and thus owning inherent biocompatibility and osseointegration properties.As such,they have been widely used in clinical orthopedics and dentistry.The new emergence of surface functionalization on metallic implants with CaP coatings shows promise for a combination of mechanical properties from metals and various biofunctions from CaPs.This review provides a brief summary of state-of-art of surface biofunctionalization on implantable metals by CaP coatings.We first glance over different types of CaPs with their coating methods and in vitro and in vivo performances,and then give insight into the representative biofunctions,i.e.osteointegration,corrosion resistance and biodegradation control,and antibacterial property,provided by CaP coatings for metallic implant materials.展开更多
The first decade of the 21st century has been labeled as "the sensing decade". The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and el...The first decade of the 21st century has been labeled as "the sensing decade". The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and electronic biosensors. A lot of works have fo- cused on the biofunctionalization of different nanomaterials, such as metal nanoparticles, semiconductor nanoparticles and carbon nanostructures, by physical adsorption, electrostatic binding, specific recognition or covalent coupling. These biofunc- tionalized nanomaterials can be used as catalysts, electronic conductors, optical emitters, carriers or tracers to obtain the ampli- fied detection signal and the stabilized recognition probes or biosensing interface. The designed signal amplification strategies have greatly promoted the development of stable, specific, selective and sensitive biosensors in different fields. This review in- troduces some novel principles and detection strategies in the area of biosensing, based on functional nanomaterials. The gen- eral methods for biofunctionalization of nanomaterials with biomolecules and their biosensing application in immunoassay of protein, DNA detection, carbohydrate analysis and cytosensing are also described.展开更多
Cell-material and cell-cell interactions represent two crucial aspects of the regulation of cell behavior.In the present study,poly(L-glutamic acid)(PLG)hydrogels were prepared by catalyst-free click crosslinking via ...Cell-material and cell-cell interactions represent two crucial aspects of the regulation of cell behavior.In the present study,poly(L-glutamic acid)(PLG)hydrogels were prepared by catalyst-free click crosslinking via a strain-promoted azide-alkyne cycloaddition(SPAAC)reaction between azido-grafted PLG(PLG-N3)and azadibenzocyclooctyne-grafted PLG(PLG-ADIBO).The bioactive peptides c(RGDfK)and N-cadherin mimetic peptide(N-Cad)were both conjugated to the PLG hydrogel(denoted PLG+RGD/N-Cad)in order to regulate cell-material and cell-cell interactions.Gelation time and storage modulus of the hydrogels were tunable through variations in the concentration of polypeptide precursors.The hydrogels degraded gradually in the presence of proteinases.The viability of bone marrow mesenchymal stem cells(BMSCs)was maintained when cultured with extracts of the hydrogels or encapsulated within the hydrogels.Degradation was observed within 10 weeks following the subcutaneous injection of hydrogel solution in rats,displaying excellent histocompatibility in vivo.The introduction of RGD into the PLG hydrogel promoted the adhesion of BMSCs onto the hydrogels.Moreover,when encapsulated within the PLG+RGD/NCad hydrogel,BMSCs secreted cartilage-specific matrix,in addition to chondrogenic gene and protein expression being significantly enhanced in comparison with BMSCs encapsulated in hydrogels without N-Cad modification.These findings suggest that these biodegradable,bioactive polypeptide hydrogels have great potential for use in 3D cell culture and in cartilage tissue engineering.展开更多
The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modificatio...The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modifications with multiple biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties are required.However,the functions of conventional bioactive coatings are limited.Thus novel biofunctional magnesium(Mg)coatings are believed to be promising candidates for surface modification of implant materials for use in bone tissue repair.By physical vapour deposition,many previous researchers have deposited Mg coatings with high purity and granular microstructure on titanium alloys,polyetheretherketone,steels,Mg alloys and silicon.It was found that the Mg coatings with high-purity could considerably control the degradation rate in the initial stage of Mg alloy implantation,which is the most important problem for the application of Mg alloy implants.In addition,Mg coating on titanium(Ti)implant materials has been extensively studied both in vitro and in vivo,and the results indicated that their corrosion behaviour and biocompatibility are promising.Mg coatings continuously release Mg ions during the degradation process,and the alkaline environment caused by Mg degradation has obvious antibacterial effects.Meanwhile,the Mg coating has beneficial effects on osteogenesis and osseointegration,and increases the new bone-regenerating ability.Mg coatings also exhibit favourable osteogenic and angiogenic properties in vitro and increased long-term bone formation and early vascularization in vivo.Inhibitory effects of Mg coatings on osteoclasts have also been proven,which play a great role in osteoporotic patients.In addition,in order to obtain more biofunctions,other alloying elements such as copper have been added to the Mg coatings.Thus,Mg-coated Ti acquired biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties.These novel multi-functional Mg coatings are expected to significantly enhance the long-term safety of bone implants for the benefit of patients.This paper gives a brief review of studies of the microstructure,degradation behaviours and biofunctions of Mg coatings,and directions for future research are also proposed.展开更多
基金supported by National Key Research and Development Program of China(2021YFA0910900)the National Natural Science Foundation of China(32222044,22104147)+5 种基金Shenzhen Municipal Science and Technology Innovation Council(RCYX20210609103823046)Youth Innovation Promotion Association CAS(2021359)Natural Science Foundation of Guangdong(2020A1515111130)Guangdong Provincial Key Laboratory of Synthetic Genomics(2019B030301006)Shenzhen Science and Technology Program(KQTD20180413181837372)Shenzhen Outstanding Talents Training Fund.
文摘Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to the health,social well-being,and economic conditions of millions of people worldwide.Therefore,there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission.Quantum dots(QDs)are typical fluorescence nanomaterials with high quantum yield,broad absorbance range,narrow and size-dependent emission,and good stability.QDs-based nanotechnology has been found to be effective method with rapid response,easy operation,high sensitivity,and good specificity,and has been widely applied for the detection of different viruses.However,until now,no systematic and critical review has been published on this important research area.Hence,in this review,we aim to provide a comprehensive coverage of various QDs-based virus detection methods.The fundamental investigations have been reviewed,including information related to the synthesis and biofunctionalization of QDs,QDs-based viral nucleic acid detection strategies,and QDs-based immunoassays.The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.
基金supported by NIH R01 DE14190(HX),R21 DE22625(HX)and R01 HL-073781(LC)the University of Maryland School of Dentistry startup fund(HX)
文摘Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）, and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement （CPC） containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34＋ cells which were reprogrammed by a single episomal vector, iPSCs were cultured to form embryoid bodies （EBs）, and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC： RGD （Arg-Gly-Asp） peptides, fibronectin （Fn）, fibronectin-like engineered polymer protein （FEPP）, extracellular matrix Geltrex, and platelet concentrate, iPSC-MSCs were seeded on five biofunctionalized CPCs： CPC-RGD, CPC-Fn, CPC- FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and coUagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering, iPSC- MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/ orthopedic repairs.
基金This study was financially supported by the National Natural Science Foundation of China(Grant Nos.52022095,51973216,51873207,51803006,and 51833010)the Provincial Health Specific Project of Jilin Province(Grant Nos.JLSWSRCZX2020-0021,2018SCZ018,and SCZSY201710)+3 种基金the Specific Project for Health Research Talents of Jilin Province(Grant No.2019SCZ025)the Science and Technology Development Program of Jilin Province(Grant Nos.20200404182YY,20200201478JC,and 20190303154SF)the Youth Innovation Promotion Association of Chinese Academy of Sciences(Grant No.2019230)and the State Key Laboratory of Advanced Technology for Materials Synthesis and Processing(Wuhan University of Technology)(Grant No.2020-KF-5).In addition,the authors are grateful to Wuhan Metware Biotechnology Co.,Ltd,Wuhan,P.R.China,for the assistance in metabolomic analysis and Dr.Di Li from the First Hospital of Jilin University,P.R.China,for the valuable discussion.
文摘Osteonecrosis is a common orthopedic disease in clinic,resulting in joint collapse if no appropriate treatment is performed in time.Core decompression is a general treatment modality for early osteonecrosis.However,effective bone regeneration in the necrotic area is still a significant challenge.This study developed a biofunctionalized composite scaffold(PLGA/nHA30VEGF)for osteonecrosis therapy through potentiation of osteoconduction,angiogenesis,and a favorable metabolic microenvironment.The composite scaffold had a porosity of 87.7%and compressive strength of 8.9 MPa.PLGA/nHA30VEGF had an average pore size of 227.6μm and a water contact angle of 56.5◦with a sustained release profile of vascular endothelial growth factor(VEGF).After the implantation of PLGA/nHA30VEGF,various osteogenic and angiogenic biomarkers were upregulated by 2-9 fold compared with no treatment.Additionally,the metabolomic and lipidomic profiling studies demonstrated that PLGA/nHA30VEGF effectively regulated the multiple metabolites and more than 20 inordinate metabolic pathways in osteonecrosis.The excellent performances reveal that the biofunctionalized composite scaffold provides an advanced adjuvant therapy modality for osteonecrosis.
基金This work was financially supported by funds from the Core Research Program 1515 of Hunan Agricultural University, the National Natural Science Foundation of China （31101268）, and Scholar Research of Kagoshima University of Japan （for De-Xing Hou）.
文摘Reactive oxygen species （ROS） can be caused by mechanical, thermal, infectious, and chemical stimuli, and their negative effects on the health of humans and other animals are of considerable concern. The nuclear factor （erythroid-derived 2）-like 2/Kelch-like ECH-associated protein 1 （Nrf2/Keap1） system plays a major role in maintaining the balance between the production and elimination of ROS via the regulation of a series of detoxifying and antioxidant enzyme gene expressions by means of the antioxidant response element （ARE）. Dietary phytochemicals, which are generally found in vegetables, fruits, grains, and herbs, have been reported to have health benefits and to improve the growth performance and meat quality of farm animals through the regulation of Nrf2-mediated phase II enzymes in a variety of ways. However, the enormous quantity of somewhat chaotic data that is available on the effects of phytochemicals needs to be properly classified according to the functions or mechanisms of phytochemicals, in this review, we first introduce the antioxidant properties of phytochemicals and their relation to the Nrf2/Keap1 system. We then summarize the effects of phytochemicals on the growth performance, meat quality, and intestinal microbiota of farm animals via targeting the Nrf2/Keap1 system. These exhaustive data contribute to better illuminate the underlying biofunctional properties of phytochemicals in farm animals.
基金Financially supported by the National Natural Science Foundation of China(Grant Nos.51973216,51873207,51803006,51673190,51603204,51673187,and 51520105004)the Science and Technology Development Program of Jilin Province(Grant Nos.20190201068JC,20170101102JC,and 20160414047GH)+2 种基金the Medical and Health Program of Jilin Province(Grant No.20190304047YY)the Youth Talents Promotion Project of Jilin Province(Grant No.181909)and the Youth Innovation Promotion Association of Chinese Academy of Sciences(Grant No.2019005).
文摘Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and an improved prognosis.Innovative design and advanced biointerface engineering have promoted the development of various nanocarriers for optimized drug delivery.Keeping in mind the biological framework of the tumormicroenvironment,biomembrane-camouflaged nanoplatforms have been a research focus,reflecting their superiority in cancer targeting.In this review,we summarize the development of various biomimetic cell membrane-camouflaged nanoplatforms for cancertargeted drug delivery,which are classified according to the membranes fromdifferent cells.The challenges and opportunities of the advanced biointerface engineering drug delivery nanosystems in cancer therapy are discussed.
基金Supported by Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support,No.20161423.
文摘Bone marrow-derived mesenchymal stem cells(BMSCs)play a critical role in the osseointegration of bone and orthopedic implant.However,osseointegration between the Ti-based implants and the surrounding bone tissue must be improved due to titanium’s inherent defects.Surface modification stands out as a versatile technique to create instructive biomaterials that can actively direct stem cell fate.Here,we summarize the current approaches to promoting BMSC osteogenesis on the surface of titanium and its alloys.We will highlight the utilization of the unique properties of titanium and its alloys in promoting tissue regeneration,and discuss recent advances in understanding their role in regenerative medicine.We aim to provide a systematic and comprehensive review of approaches to promoting BMSC osteogenesis on the orthopedic implant surface.
文摘Cordyceps is treasured entomopathogenic fungi that have been used as antitumor,immunomodulating,antioxidant,and pro-sexual agent.Cordyceps,also called DongChongXiaCao in Chinese,Yartsa Gunbu(Tibetan),means winter worm-summer grass.Natural Cordyceps sinensis with parasitic hosts is difficult to be collected and the recent findings on its potential pharmacological functions,resulted in skyrocketing prices.Therefore,finding a mass-production method or an alternative for C.sinensis products is a top-priority task.In this review,we describe current status of Cordyceps research and its recent developments in Taiwan.The content and pharmacological activities of four major industrial species of Cordyceps(C.sinensis,Cordyceps militaris,Cordyceps cicadae and Cordyceps sobolifera)used in Taiwan,were reviewed.Moreover,we highlighted the effect of using different methods of fermentation and production on the morphology and chemical content of Cordyceps sp.Finally,we summarized the bottle-necks and challenges facing Cordyceps research as well as we proposed future road map for Cordyceps industry in Taiwan.
基金The project is supported by Natural Science Foundation of China
文摘In this paper diamond like carbon (DLC) /stainless steel biofunctionally gradient coatings have been prepared with two target magnetron cosputtering to solve the poor adhesion of DLC having excellent haemocompatibility to implant stainless steel having high strength and toughness.It has been Known from the experimental results that the adhesion of DLC coated to implant stainless steel have been significantly improved with the gradient interlayers,and biofuntionally gradient materials having excellent haemocompatibilty,strength and toughness have been prepared successively.
基金funded by National Natural Science Foundation of China,grant 81701020National Natural Science Foundation of China,grant 82071081+1 种基金Shanghai Municipal Health and Family Planning Commission,grant 201740035China Postdoctoral Science Foundation,grant 2023M742318。
文摘Mesoporous bioactive glasses(MBGs),which belong to the category of modern porous nanomaterials,have garnered significant attention due to their impressive biological activities,appealing physicochemical properties,and desirable morphological features.They hold immense potential for utilization in diverse fields,including adsorption,separation,catalysis,bioengineering,and medicine.Despite possessing interior porous structures,excellent morphological characteristics,and superior biocompatibility,primitive MBGs face challenges related to weak encapsulation efficiency,drug loading,and mechanical strength when applied in biomedical fields.It is important to note that the advantageous attributes of MBGs can be effectively preserved by incorporating supramolecular assemblies,miscellaneous metal species,and their conjugates into the material surfaces or intrinsic mesoporous networks.The innovative advancements in these modified colloidal inorganic nanocarriers inspire researchers to explore novel applications,such as stimuli-responsive drug delivery,with exceptional in-vivo performances.In view of the above,we outline the fabrication process of calcium-silicon-phosphorus based MBGs,followed by discussions on their significant progress in various engineered strategies involving surface functionalization,nanostructures,and network modification.Furthermore,we emphasize the recent advancements in the textural and physicochemical properties of MBGs,along with their theranostic potentials in multiple cancerous and non-cancerous diseases.Lastly,we recapitulate compelling viewpoints,with specific considerations given from bench to bedside.
基金supported by National Institutes of Health[Grant number R01HL140562]。
文摘Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of metallic implants for high-performance biofunctions.Calcium phosphates(CaPs)are the major inorganic component of bone tissues,and thus owning inherent biocompatibility and osseointegration properties.As such,they have been widely used in clinical orthopedics and dentistry.The new emergence of surface functionalization on metallic implants with CaP coatings shows promise for a combination of mechanical properties from metals and various biofunctions from CaPs.This review provides a brief summary of state-of-art of surface biofunctionalization on implantable metals by CaP coatings.We first glance over different types of CaPs with their coating methods and in vitro and in vivo performances,and then give insight into the representative biofunctions,i.e.osteointegration,corrosion resistance and biodegradation control,and antibacterial property,provided by CaP coatings for metallic implant materials.
基金the National Basic Research Program of China (2010CB732400)the National Natural Science Foundation of China (20821063 & 20875044)the Natural Science Foundation of Jiangsu (BK2008014)
文摘The first decade of the 21st century has been labeled as "the sensing decade". The functional nanomaterials offer excellent platforms for fabrication of sensitive biosensing devices, including optical and electronic biosensors. A lot of works have fo- cused on the biofunctionalization of different nanomaterials, such as metal nanoparticles, semiconductor nanoparticles and carbon nanostructures, by physical adsorption, electrostatic binding, specific recognition or covalent coupling. These biofunc- tionalized nanomaterials can be used as catalysts, electronic conductors, optical emitters, carriers or tracers to obtain the ampli- fied detection signal and the stabilized recognition probes or biosensing interface. The designed signal amplification strategies have greatly promoted the development of stable, specific, selective and sensitive biosensors in different fields. This review in- troduces some novel principles and detection strategies in the area of biosensing, based on functional nanomaterials. The gen- eral methods for biofunctionalization of nanomaterials with biomolecules and their biosensing application in immunoassay of protein, DNA detection, carbohydrate analysis and cytosensing are also described.
基金supported by the National Natural Science Foundation of China(51973218,51622307,21574127,51520105004)the Youth Innovation Promotion Association,Chinese Academy of Sciences。
文摘Cell-material and cell-cell interactions represent two crucial aspects of the regulation of cell behavior.In the present study,poly(L-glutamic acid)(PLG)hydrogels were prepared by catalyst-free click crosslinking via a strain-promoted azide-alkyne cycloaddition(SPAAC)reaction between azido-grafted PLG(PLG-N3)and azadibenzocyclooctyne-grafted PLG(PLG-ADIBO).The bioactive peptides c(RGDfK)and N-cadherin mimetic peptide(N-Cad)were both conjugated to the PLG hydrogel(denoted PLG+RGD/N-Cad)in order to regulate cell-material and cell-cell interactions.Gelation time and storage modulus of the hydrogels were tunable through variations in the concentration of polypeptide precursors.The hydrogels degraded gradually in the presence of proteinases.The viability of bone marrow mesenchymal stem cells(BMSCs)was maintained when cultured with extracts of the hydrogels or encapsulated within the hydrogels.Degradation was observed within 10 weeks following the subcutaneous injection of hydrogel solution in rats,displaying excellent histocompatibility in vivo.The introduction of RGD into the PLG hydrogel promoted the adhesion of BMSCs onto the hydrogels.Moreover,when encapsulated within the PLG+RGD/NCad hydrogel,BMSCs secreted cartilage-specific matrix,in addition to chondrogenic gene and protein expression being significantly enhanced in comparison with BMSCs encapsulated in hydrogels without N-Cad modification.These findings suggest that these biodegradable,bioactive polypeptide hydrogels have great potential for use in 3D cell culture and in cartilage tissue engineering.
基金supported by the National Key Research and Development Program of China(Nos.2016YFC1101804,2016YFC1100604)National Natural Science Foundation of China(Nos.51971222,51631009)+2 种基金Natural Science Foundation of Liaoning Province of China(No.2019-MS-326)Dongguan Innovative Research Team Program of China(No.2020607234007)China Postdoctoral Science Foundation(No.2021M690494).
文摘The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the clinic.To achieve high osseointegration,surface modifications with multiple biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties are required.However,the functions of conventional bioactive coatings are limited.Thus novel biofunctional magnesium(Mg)coatings are believed to be promising candidates for surface modification of implant materials for use in bone tissue repair.By physical vapour deposition,many previous researchers have deposited Mg coatings with high purity and granular microstructure on titanium alloys,polyetheretherketone,steels,Mg alloys and silicon.It was found that the Mg coatings with high-purity could considerably control the degradation rate in the initial stage of Mg alloy implantation,which is the most important problem for the application of Mg alloy implants.In addition,Mg coating on titanium(Ti)implant materials has been extensively studied both in vitro and in vivo,and the results indicated that their corrosion behaviour and biocompatibility are promising.Mg coatings continuously release Mg ions during the degradation process,and the alkaline environment caused by Mg degradation has obvious antibacterial effects.Meanwhile,the Mg coating has beneficial effects on osteogenesis and osseointegration,and increases the new bone-regenerating ability.Mg coatings also exhibit favourable osteogenic and angiogenic properties in vitro and increased long-term bone formation and early vascularization in vivo.Inhibitory effects of Mg coatings on osteoclasts have also been proven,which play a great role in osteoporotic patients.In addition,in order to obtain more biofunctions,other alloying elements such as copper have been added to the Mg coatings.Thus,Mg-coated Ti acquired biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties.These novel multi-functional Mg coatings are expected to significantly enhance the long-term safety of bone implants for the benefit of patients.This paper gives a brief review of studies of the microstructure,degradation behaviours and biofunctions of Mg coatings,and directions for future research are also proposed.
