The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the FMVZ-BUAP Gen...The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the FMVZ-BUAP Genetics and Reproduction Laboratory. 5 stallions were used. Each sample contained 1 × 10<sup>6</sup> sperm, the PRRS virus strain was ATCC-VR-2332 (0, 10<sup>2</sup>, 10<sup>4</sup> and 10<sup>6</sup> copies of RNA/mL in triplicate), it was observed daily at the CASA;Hamilton Thorne<sup>®</sup>. Cells with MT (P < 0.05) on days 1, 3, 5, 7 and 10 of evaluation with 201 ± 7.3, 167 ± 10.1, 165 ± 14.6, 134 ± 8.2 and 120 ± 8.8, respectively. The % MP between control and virus concentrations (P ≥ 0.05). The LCV on day 1 and 7 PI at 10X<sup>2</sup> and 10X<sup>6</sup> (P < 0.05) vs control. In the Correlation Matrix, where it is observed that there is a correlation between VSL and VAP, VSL and VCL, VCL and ALH, VAP with ALH. There is a correlation of VSL and ALH, STR and ALH. In this study there were (P ≤ 0.01) in the VCL, in the concentrations (10<sup>2</sup>) 162.81 ± 10.65 and (10<sup>6</sup>) 177.12 ± 5.77 vs 193.04 ± 4.62 of control. This indicates that altering these parameters would be related to fertility and the PRRS virus affects the LCV. Regarding the VSL, it was observed that the sperm infected with viruses 10<sup>2</sup>, 10<sup>4</sup> and 10<sup>6</sup> of 48.00 ± 3.38, 49.88 ± 1.83 and 50.55 ± 2.24 Vs. 56.66 ± 1.68 of control respectively, the control would have greater possibilities of fertilizing the oocyte. In this study, it was found (P ≤ 0.01) in the VAP with 102 of 77.26 ± 5.16, 10<sup>4</sup> with 83.35 ± 2.41 and 10<sup>6</sup> with 81.29 ± 3.14 vs the control with 90.56 ± 2.07. Regarding the ALH there is (P < 0.05) a 10<sup>4</sup> with 8.70 ± .26 and 10<sup>6</sup> with 9.64 ± 0.23 vs control 8.50 ± 0.27. The presence of different concentrations of PRRSV in boar semen induces changes in different types of sperm motility. Infection of ejaculates with the PRRS virus affects sperm motility on days 1, 3, 5, 7, and 10 post-infections.展开更多
Objective:To determine the prevalence of bacteriospermia,the bacterial load,and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operat...Objective:To determine the prevalence of bacteriospermia,the bacterial load,and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operators.Methods:Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte,Philippines and were subjected to standard bacteriological culture and identification,including a survey of potentially associated factors.Prevalence and bacterial count were determined accordingly,while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering technique.Results:One hundred percent contamination with a bacterial count of(2.01±0.38)×10^(3) CFU/mL was observed.At least 73.33%of the samples were positive for Bacillus spp.,while other identified isolates included Enterobacter spp.,Staphylococcus spp.,E.coli,Pseudomonas spp.,Citrobacter spp.,and Klebsiella spp.Conclusions:Despite the high prevalence of bacteriospermia,the bacterial count is low.Nevertheless,on-farm practices on boar health and management,semen collection,and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation.展开更多
Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that ...Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments,which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm(i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction.Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm.However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor(PFE) than in good(GFE) freezability ejaculates in both pre-frozen(1.00 INT·mm^2± 0.14 INT·mm^2 vs.0.72 INT·mm^2± 0.15 INT·mm^2;P < 0.05) and post-thawed(0.96 INT·mm^2± 0.20 INT·mm^2 vs. 70 INT·mm^2± 0.19 INT·mm^2;P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species(ROS) production, and high membrane lipid disorder postthaw(P < 0.05).Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm.展开更多
Economic losses caused by wild boars(Sus scrofa)continue to increase in the Three Gorges Reservoir Area.We selected 206 farmers in Wuqiaohe Basin as survey respondents using random stratified sampling.The distribution...Economic losses caused by wild boars(Sus scrofa)continue to increase in the Three Gorges Reservoir Area.We selected 206 farmers in Wuqiaohe Basin as survey respondents using random stratified sampling.The distribution and impacts of wild boars were examined using a questionnaire survey and spatial analysis.The results showed that:(1)The density of farmer settlements is low and most peasants’households are located on the forest edge.Due to large-scale labor migration,a high proportion of farmland(25.85%)has been abandoned and an even higher proportion of farmland(40.48%)has been returned to forest.(2)The probability of wild boar damage to peasant households was 67.96%.Yields of corn and sweet potato decreased by 24.87%and 28.24%,respectively,with a total economic loss of approximately 2,590,100 RMB/yr in the Wuqiaohe basin.Sixty-five percent of the affected cultivatedlands were located within approximately 150 m of the forest edges.(3)The wild boar damage coefficient was significantly and negatively correlated with the distance between the peasants’household and the forest edge,the density of the settlements,and the area of cultivated land.The wild boar damage coefficient shows a significant positive correlation with the area of farmland returned to forest.(4)The damage activity caused by wild boars began to occur7-8 years ago,and the destruction has been more frequently and seriously observed in the last 4-5 years.(5)We make suggestions for wild boar control and management measures including developing a compensation policy.The compensation standard is related to the land area returning from farmland to forests and the actual cultivated land area.展开更多
[Objectives]This study was conducted to compare and analyze the accuracy of computer-aided sperm analysis(CASA)and manual method for detecting the quality of fresh boar semen at room temperature.[Methods]Statistical m...[Objectives]This study was conducted to compare and analyze the accuracy of computer-aided sperm analysis(CASA)and manual method for detecting the quality of fresh boar semen at room temperature.[Methods]Statistical methods such as analysis of variance,ZB score and Z score were used to compare the accuracy of five different brands of CASA systems and manual method to detect the vitality and density of fresh boar semen at room temperature.[Results]After setting the parameters of five CASA systems the same as follows:VCL(curvilinear velocity),VSL(straight-line velocity)≥5μm/s,STR(straightness)=VSL(straight-line velocity)/VAP(average path velocity)≥25%,the sperm motility of six parts of boar semen was tested at normal temperature using different brands of special fixed-volume slides with a uniform chamber height[(20±2)μm].There were no significant differences in sperm vitality detected by the five CASA systems(P>0.05).The ZB scores of the vitality obtained by observers or instrument engineers who did not have a job certificate from the quality inspection department showed that the results of three observers or instrument engineers were unsatisfactory(∣ZB∣>3),but there were no significant differences in vitality between the CASA systems and the inspector with a job certificate(P>0.05).Regarding sperm density detection,when the sperm density was less than 280×106/ml,there were no significant differences between the results displayed by the instruments and the results of the manual hemocytometer counting(P>0.05).[Conclusions]The accuracy of the CASA systems set to uniform parameters was consistent with the accuracy of the visual vitality obtained by an inspector with a job certificate.When the semen was diluted with 3%NaCl solution to a sperm density<280×10^6/ml,the sperm density detected by the CASA systems was consistent in reliability with that obtained by the hemocytometer detection.The CASA systems are faster and more efficient and objective than manual detection,and have the advantages of strong operability and easy promotion.展开更多
Hybrid wild boar husbandry is an important component of livestock production in Northeast China.However,the current disease situation of these animals is largely unknown due to a lack of disease surveillance.The prese...Hybrid wild boar husbandry is an important component of livestock production in Northeast China.However,the current disease situation of these animals is largely unknown due to a lack of disease surveillance.The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China.Between September 2015 to December 2016,169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin,Inner Mongolia and Heilongjiang provinces.ELISA detected serum antibodies against classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),pseudorabies virus(PRV),porcine circovirus type 2(PCV2)and Japanese encephalitis virus(JEV),but not against African swine fever virus(ASFV),with PCV2 having the highest seropositive rate(87.2–100%in different farms).RT-PCR or PCR performed on the processed samples detected only PCV2,with 33.1%(56/169)of blood samples and 32.8%(20/61)of spleen samples being positive,respectively,indicating widespread PCV2 infection in hybrid wild boars.Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a,PCV2b and PCV2d,with nucleotide and deduced amino acid homologies of 88.5–100%and 88.1–100%,respectively.展开更多
Background: The aim of the study was to analyze the lifetime and culling reasons for boars used in insemination centers(AI centers).Methods: The data collected from 355 culled boars from 1998 to 2013 included: age at ...Background: The aim of the study was to analyze the lifetime and culling reasons for boars used in insemination centers(AI centers).Methods: The data collected from 355 culled boars from 1998 to 2013 included: age at start of semen collection,boar herd life, culling reason, daily gain and lean meat content, and number of ejaculates not meeting sales requirements after dilution. Culling reasons were divided into 7 groups: low semen value(LSV), low or lack of libido(LL), leg problems(LP), infectious diseases(ID), old age(OA), reduced demand for semen from the given boar(RD),and others(OT).Results: The most common culling reasons for boars were LSV(23.7%) and RD(22.5%). It was observed that the lowest daily gains were noted in boars culled due to OA. Boars culled due to OA and RD were maintained in production for the longest time(over 1000 d), for LSV and ID retention was about 700 d, and due to LL below 400 d. The survival probability was over 0.9 until 1.5 yr, and just over 0.2 until 4 yr. The highest relative frequency was observed in the 36 ^(th) and 42 ^(nd) mo of life(over 16%). Hazard risk analysis revealed a more than 10 times higher risk of culling in the case of LL, ID or OT, in comparison to OA.Conclusions: The results can be used as a direct point of reference for the identification of emerging problems in AI boar exploitation and the development of an appropriate culling policy in AI centers.展开更多
Toll-like receptor(TLR)4 plays an important role in the innate immune system and has been involved in resistance/susceptibility to a number of diseases as revealed by studies in human and other domestic animals.Wild b...Toll-like receptor(TLR)4 plays an important role in the innate immune system and has been involved in resistance/susceptibility to a number of diseases as revealed by studies in human and other domestic animals.Wild boar survives in natural environment without artificial interference and may be different from domestic pig in innate immune system.Here,the complete coding sequence of TLR4 and TLR4A was cloned in wild boar,and two other alternative splicing variants,TLR4B and TLR4C,were obtained.Compared to the counterpart from domestic pig(Gen Bank No.AJ628065),there were five SNPs,c.510T>C,c.960A>G,c.962A>G,c.1605T>G and c.1824A>G,in the coding sequence of wild boar TLR4A gene.TLR4 gene was expressed in all the tissues from wild boar studied with the most abundance in spleen tissue,and m RNA level was significantly lower in spleen from wild boar than that from Min pig.The allele distribution was significantly different at polymorphic loci c.962G>A and c.1027C>A(p<0.01)between wild boar and Min pig.The results would contribute to understand the innate immune system in wild boar.展开更多
To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents(Diluen...To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents(DiluentⅠ, DiluentⅡ and Diluent Ⅲ) and three boar breed semens(Yorkshire, Landrace and Duroc) were utilized. The sperm motility, effective survival time, survival index, catalase(CAT), the total anti-oxidative capacity(T-AOC) and malondialdehyde(MDA) levels were evaluated. The results showed that there were significant interaction effects between diluents and breeds on the boar sperm motility(P<0.001), survival time(P<0.001), CAT levels(P<0.001) and T-AOC levels(P<0.001), but neither effects nor interaction effects between diluents and breeds on survival index(P>0.05). All of the parameters varied significantly with the increase of the storage time(P<0.001). The survival time increased 12.9% in Yorkshire boar semen diluted with Diluent Ⅲ than with DiluentⅡ, while the survival time increased 6.6% in Landrace boar semen diluted with DiluentⅡ than with Diluent Ⅲ. Both CAT and T-AOC levels were significantly positive correlated with sperm motility in all the three boar breeds(P<0.001), while MDA levels were significantly negative correlated with sperm motility(P<0.001). These results indicated that Diluent Ⅲ and DiluentⅡ were the optimal commercial diluents for Yorkshire and Landrace boar semen stored at 17℃, respectively.展开更多
Background: Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main...Background: Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility.Objectives: To examine chilling injury and the number and type of bacteria in boar semen stored at 5 ℃ in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions.Material and methods: Boar ejaculates were extended with AndroStar~? Premium, stored at 17 ℃ with and at 5 ℃ without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study.Results: Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher(P < 0.05) up until 72 h in sperm stored at 5 ℃ compared to 17 ℃ but did not differ after 144 h.After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 ℃, bacteria counts were below 103 CFU/mL and the bacteria spectrum was similar to that of raw semen. In 88% of 34 boars,cooled semen fulfilled the requirements for insemination. Fertility was high and did not differ(P > 0.05) between sow groups inseminated with semen stored antibiotic-free at 5 ℃ and semen stored at 17 ℃ with antibiotics.Conclusion: Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable.展开更多
Wild boar is one of the most important beast resources.It plays an important role in the maintenance of biological diversity.The genetic resources of wild boar can not only protect the genetic resources,but also impro...Wild boar is one of the most important beast resources.It plays an important role in the maintenance of biological diversity.The genetic resources of wild boar can not only protect the genetic resources,but also improve the formation of new breeds in pigs.This paper summarized the advance on the main biological characteristics of wild boars,evolutionary origin between wild boars and domesticated pigs,and development and utilization of wild boars aimed to provide further insight into wild boar's genetic research and its resource protection.展开更多
Objective:To characterize the semen of three genetic types of boars(local,improved and Large White)reared in Benin.Methods:Semen of local,improved and Large White boars reared in Benin were collected using the gloved ...Objective:To characterize the semen of three genetic types of boars(local,improved and Large White)reared in Benin.Methods:Semen of local,improved and Large White boars reared in Benin were collected using the gloved hand method and analyzed to determine volume,pH,concentration,mobility,motility,and morphology.The effect of the genetic type of boar on semen characteristics was aslo studied.Results:Duration of ejaculation and semen volume of Large White boar were significantly higher than those of local and improved boars(P<0.05).The semen of improved boars had a higher motility score than that of Large White and local boars(P<0.001).The semen of local boars was more concentrated in the spermatozoa than that of improved and Large White boars(P<0.05).The proportion of spermatozoa of improved boars with normal morphology(93.6%)was significantly higher than that of local(82.2%)and Large White boars(81.6%)(P<0.001).The proportion of spermatozoa with folded tails in the semen of Large White boars(9.2%)was significantly higher than that observed in improved(1.8%)and local(5.0%)boars(P<0.001).The proportion of spermatozoa with proximal cytoplasmic droplets in semen of improved boars(2.7%)was significantly lower than that in Large White(6.8%)and local(9.7%)boars(P<0.001).The local(1.5%)and Large White boars(1.1%)showed more spermatozoa with distal cytoplasmic droplets in their semen compared to the improved boars(0.4%).Conclusions:The semen characteristics of pigs reared in Benin vary from one genetic type to another.Each genetic type has a strong point.The Large White boar produces larger semen,the local boar produces more concentrated semen and the improved boar produces spermatozoa that are morphologically better.The semen of these three genetic types can be used in artificial insemination but the improved boar's semen is more recommended.展开更多
The identification of superior sire is largely dependent on the semen quality evaluations, currently through computer-aided sperm class analyser (CASA). Immunoassays present a viable method for analysis of proteins as...The identification of superior sire is largely dependent on the semen quality evaluations, currently through computer-aided sperm class analyser (CASA). Immunoassays present a viable method for analysis of proteins associated with fertility, such as osteopontin. We have targeted osteopontin at an upstream epitope at position 249 - 267 amino acids and a downstream epitope at position 59 - 74 amino acids of full osteopontin protein in raw, BTS, Kobidil, Tris and Citrate liquid preserved Large white and Kolbroek boar semen. The Genetool analysis software was used to quantify the amount of osteopontin detected. Three boars per breed were used and experiments were repeated five times. Our results revealed that the downstream 15 amino acid epitope was more sensitive to osteopontin antibody. Between total motility and osteopontin, Kolbroek boar semen preserved in Kobidil, Citrate, BTS and Tris extenders revealed positive correlations of 0.564, 0.471, -0.960 and 0.330, respectively. Large white boars semen showed correlations of -0.043, 0.655, 0.778, and 0.202 Kobidil, Citrate, BTS and Tris, respectively. For rapid motility and osteopontin, Kolbroek boar semen revealed positive correlations of 0.842, 0.601, 0.164 and 0.202, respectively while large white boars semen showed correlations of -0.909, -0.144, -0.210, and 0.089, respectively. Only BTS and Kobidil showed high negative correlations between osteopontin and viability in Kolbroek (-0.975) and Large white (-0.746) boar semen, respectively. No significant correlations with osteopontin were observed when Tris extender was used in both breeds. In conclusion, Kolbroek boar semen requires osteopontin in Kobodil extender while large white boar semen requires osteopontin in BTS extender.展开更多
Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of...Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of the fatty acid source, level of inclusion and breed of boars tested. Three groups of 87 boars each, from two genetic lines (PIC 337 and PIC 800), were assigned to treatment based on age, mean sperm production (previous 12 weeks), and body condition score. All boars received a corn-soybean meal diet with a commercial fish oil supplement providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/boar/day of docosahexaenoic acid (DHA) as a preconditioning diet. On 10-Aug., 2020, the DHA source was changed to a stabilized starch imbedded source of refined fish oil (Salmate®), providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">test diet. Two additional levels providing 2.38 and 2.94</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA were fed for a 9 week pretreatment period and during the test period. Salmate® fed at 2.38</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA resulted in a reduction in the number of rejected ejaculates (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.045) by 7.5% and 6.4% compared to the lowest and highest inclusion rates, respectively. There w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> no treatment</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> by genetic line interactions. A retrospective study of semen production and quality of 77 boars on the Salmate® diet containing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d DHA was done to compare to the original source of DHA at the same inclusion level. There were no differences in semen quality parameters between the 2 lipid sources. Ejaculate volume increased from 177.9 ml to 233.4 ml (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) and total sperm cells per ejaculate increased from 69.7 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> to 82.0 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) due to substitution of Salmate®. Adding Salmate® at 2.38 g/b/d resulted in a lower number of rejected ejaculates per boar by 7.5% and 6.4% vs. 1.83 and 2.94 g/b/d, respectively, and boars fed Salmate® at 1.83 g/b/d produced 17% more doses than the competing product.展开更多
This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 a...This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.展开更多
After the famous“Rainforest Trilogy”such as Monkey Cup and Elephants,Zhang Guixing,a Malaysian Chinese writer in Taiwan,recently published another Nanyang story Boar Crossing the River,in the background of anti-Japa...After the famous“Rainforest Trilogy”such as Monkey Cup and Elephants,Zhang Guixing,a Malaysian Chinese writer in Taiwan,recently published another Nanyang story Boar Crossing the River,in the background of anti-Japanese War,focusing on the massacres and resistance of the Chinese in Krokop,a Malaysian Chinese village in Borneo from 1941 to 1945 according to a list of“Committee for the Relief of Refugees of the Motherland”.In this novel,Zhang Guixing changes the accustomed narrative mode of individual family history,expands the description to the group images of the Chinese in Krokop,highlights their patriotic sacrifice,and saves the reality of the slaughter of the compatriots by magic means such as the myths and legends of Borneo and masks in children’s imagination.It is also noteworthy that the novel talks about the relationship between individual freedom and national identity in depth,and describes the tragic fate of cross-national love in the period of national opposition.These nation-state discourses in Boar Crossing the River are Zhang Guixing’s biggest transitions when missing in the literary world for 17 years,which indicate a landmark progress in his spiritual history.展开更多
Sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane;the acrosome reaction (AR) is an exocytotic event induced by calcium influx, e...Sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane;the acrosome reaction (AR) is an exocytotic event induced by calcium influx, enabling the spermatozoa to penetrate the zona pellucida. These processes can be achieved only if the spermatozoa have good viability;indeed, determination of sperm viability is used for the assessment of semen quality. Membrane integrity and mitochondrial activity are important viability parameters of spermatozoa and fluorescent techniques based on membrane permeability to dyes have been developed to determine these parameters. The aim of this work was to determine the viability of boar sperm (fresh, one hour of capacitation induction and 20 min of AR induction) by flow cytometry using propidium iodide (PI) (1.25 μg/mL) and rhodamine 123 (R123) (0.20 μg/mL). Aliquots of 5 × 105 sperm were incubated with each fluorochrome separately and simultaneously for 10 or 20 min, respectively, at 38℃. The proportion of labeled spermatozoa and their fluorescence intensities were measured using a flow cytometer. The fluorescence index (FI) with PI gradually increased during the incubation and we found significant differences between all the groups. With R123, the FI increased in the capacitated sperm but decreased in the acrosome-reacted sperm, with significant differences between the fresh and capacitated spermatozoa. Our results suggest that the increase in the R123 fluorescence intensity in capacitated spermatozoa is due to changes in the mitochondrial membrane activity because the spermatozoa experienced changes in membrane fluidity and flagellar activation during capacitation. The use of fluorochromes and flow cytometry is a good tool for monitoring many markers of sperm function. Although capacitation and AR processes have been well studied, there is still much information to be elucidated with regard to these complex processes.展开更多
Background: Follicle-stimulating hormone(FSH) is a gonadotropin synthesized and secreted by the pituitary gland.FSH stimulates follicle development and maturation in females. It also plays an important role in spermat...Background: Follicle-stimulating hormone(FSH) is a gonadotropin synthesized and secreted by the pituitary gland.FSH stimulates follicle development and maturation in females. It also plays an important role in spermatogenesis in males, including humans and mice. However, the effects of FSH on male pigs are largely unknown. In this study,we generated transgenic pigs to investigate the effects of FSHα/β overexpression on reproductive traits in boars.Results: After five transgenic F_0 founders were crossed with wide-type pigs, 193 F_1 animals were obtained. Of these,96 were confirmed as transgenic. FSHα and FSHβ m RNAs were detected only in pituitary tissue. Transgenic boars exhibited significantly higher levels of FSHα and FSHβ m RNA, serum FSH, and serum testosterone, compared to ful-sib non-transgenic boars. Significant increases in testis weight, vas deferens diameter, seminiferous tubule diameter,and the number of Leydig cel s were observed, suggesting that the exogenous FSHα/β affects reproductive traits. Final y,transgenic and non-transgenic boars had similar growth performance and biochemical profiles.Conclusions: Pituitary-specific overexpression of FSHα/β genes is likely to impact reproductive traits positively, as indicated by enhancements in serum testosterone level, testis weight, the development of vas deferens, seminiferous tubules, and Leydig cells in transgenic boars. A high level of serum FSH induces secretion of serum testosterone,possibly by boosting the number of Leydig cells, which presumably increases the libido and the frequency of sexual activity in transgenic boars. Our study provides a preliminary foundation for the genetic improvement of reproductive traits in male pigs.展开更多
To explore the correlation of evaluation methods on boar semen quality,the motility,PI staining,hypotonic swelling test,Rh123 staining and FITC- PNA staining of boar sperm were checked in the study,and its correlation...To explore the correlation of evaluation methods on boar semen quality,the motility,PI staining,hypotonic swelling test,Rh123 staining and FITC- PNA staining of boar sperm were checked in the study,and its correlation with IVF was statistically calculated. The results showed that various indicators had strong linear correlation with each other,and the correlation degree between acrosome integrity and IVF rate was the highest,indicating that acrosome integrity was superior to other indicators in predicting IVF rate.展开更多
文摘The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the FMVZ-BUAP Genetics and Reproduction Laboratory. 5 stallions were used. Each sample contained 1 × 10<sup>6</sup> sperm, the PRRS virus strain was ATCC-VR-2332 (0, 10<sup>2</sup>, 10<sup>4</sup> and 10<sup>6</sup> copies of RNA/mL in triplicate), it was observed daily at the CASA;Hamilton Thorne<sup>®</sup>. Cells with MT (P < 0.05) on days 1, 3, 5, 7 and 10 of evaluation with 201 ± 7.3, 167 ± 10.1, 165 ± 14.6, 134 ± 8.2 and 120 ± 8.8, respectively. The % MP between control and virus concentrations (P ≥ 0.05). The LCV on day 1 and 7 PI at 10X<sup>2</sup> and 10X<sup>6</sup> (P < 0.05) vs control. In the Correlation Matrix, where it is observed that there is a correlation between VSL and VAP, VSL and VCL, VCL and ALH, VAP with ALH. There is a correlation of VSL and ALH, STR and ALH. In this study there were (P ≤ 0.01) in the VCL, in the concentrations (10<sup>2</sup>) 162.81 ± 10.65 and (10<sup>6</sup>) 177.12 ± 5.77 vs 193.04 ± 4.62 of control. This indicates that altering these parameters would be related to fertility and the PRRS virus affects the LCV. Regarding the VSL, it was observed that the sperm infected with viruses 10<sup>2</sup>, 10<sup>4</sup> and 10<sup>6</sup> of 48.00 ± 3.38, 49.88 ± 1.83 and 50.55 ± 2.24 Vs. 56.66 ± 1.68 of control respectively, the control would have greater possibilities of fertilizing the oocyte. In this study, it was found (P ≤ 0.01) in the VAP with 102 of 77.26 ± 5.16, 10<sup>4</sup> with 83.35 ± 2.41 and 10<sup>6</sup> with 81.29 ± 3.14 vs the control with 90.56 ± 2.07. Regarding the ALH there is (P < 0.05) a 10<sup>4</sup> with 8.70 ± .26 and 10<sup>6</sup> with 9.64 ± 0.23 vs control 8.50 ± 0.27. The presence of different concentrations of PRRSV in boar semen induces changes in different types of sperm motility. Infection of ejaculates with the PRRS virus affects sperm motility on days 1, 3, 5, 7, and 10 post-infections.
基金funded by the DOST-Philippine Council for Agriculture,Aquatic and Natural Resources Research and Development(PCAARRD)through the Visayas State University(Project Code:20201050-1.93)。
文摘Objective:To determine the prevalence of bacteriospermia,the bacterial load,and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operators.Methods:Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte,Philippines and were subjected to standard bacteriological culture and identification,including a survey of potentially associated factors.Prevalence and bacterial count were determined accordingly,while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering technique.Results:One hundred percent contamination with a bacterial count of(2.01±0.38)×10^(3) CFU/mL was observed.At least 73.33%of the samples were positive for Bacillus spp.,while other identified isolates included Enterobacter spp.,Staphylococcus spp.,E.coli,Pseudomonas spp.,Citrobacter spp.,and Klebsiella spp.Conclusions:Despite the high prevalence of bacteriospermia,the bacterial count is low.Nevertheless,on-farm practices on boar health and management,semen collection,and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation.
基金support from Ministry of Science,Innovation and Universities,Spain(Grants:RYC-2014-15581,AGL2017–88329-R and FJCI-2017-31689)European Commission(H2020-MSCA-IF-79212)
文摘Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments,which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm(i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction.Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm.However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor(PFE) than in good(GFE) freezability ejaculates in both pre-frozen(1.00 INT·mm^2± 0.14 INT·mm^2 vs.0.72 INT·mm^2± 0.15 INT·mm^2;P < 0.05) and post-thawed(0.96 INT·mm^2± 0.20 INT·mm^2 vs. 70 INT·mm^2± 0.19 INT·mm^2;P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species(ROS) production, and high membrane lipid disorder postthaw(P < 0.05).Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm.
基金supported by the 135 strategic program of the institute of mountain hazards and environment, CAS (NO. SDS-135-1703)national natural science foundation of China (No. 41401664)
文摘Economic losses caused by wild boars(Sus scrofa)continue to increase in the Three Gorges Reservoir Area.We selected 206 farmers in Wuqiaohe Basin as survey respondents using random stratified sampling.The distribution and impacts of wild boars were examined using a questionnaire survey and spatial analysis.The results showed that:(1)The density of farmer settlements is low and most peasants’households are located on the forest edge.Due to large-scale labor migration,a high proportion of farmland(25.85%)has been abandoned and an even higher proportion of farmland(40.48%)has been returned to forest.(2)The probability of wild boar damage to peasant households was 67.96%.Yields of corn and sweet potato decreased by 24.87%and 28.24%,respectively,with a total economic loss of approximately 2,590,100 RMB/yr in the Wuqiaohe basin.Sixty-five percent of the affected cultivatedlands were located within approximately 150 m of the forest edges.(3)The wild boar damage coefficient was significantly and negatively correlated with the distance between the peasants’household and the forest edge,the density of the settlements,and the area of cultivated land.The wild boar damage coefficient shows a significant positive correlation with the area of farmland returned to forest.(4)The damage activity caused by wild boars began to occur7-8 years ago,and the destruction has been more frequently and seriously observed in the last 4-5 years.(5)We make suggestions for wild boar control and management measures including developing a compensation policy.The compensation standard is related to the land area returning from farmland to forests and the actual cultivated land area.
文摘[Objectives]This study was conducted to compare and analyze the accuracy of computer-aided sperm analysis(CASA)and manual method for detecting the quality of fresh boar semen at room temperature.[Methods]Statistical methods such as analysis of variance,ZB score and Z score were used to compare the accuracy of five different brands of CASA systems and manual method to detect the vitality and density of fresh boar semen at room temperature.[Results]After setting the parameters of five CASA systems the same as follows:VCL(curvilinear velocity),VSL(straight-line velocity)≥5μm/s,STR(straightness)=VSL(straight-line velocity)/VAP(average path velocity)≥25%,the sperm motility of six parts of boar semen was tested at normal temperature using different brands of special fixed-volume slides with a uniform chamber height[(20±2)μm].There were no significant differences in sperm vitality detected by the five CASA systems(P>0.05).The ZB scores of the vitality obtained by observers or instrument engineers who did not have a job certificate from the quality inspection department showed that the results of three observers or instrument engineers were unsatisfactory(∣ZB∣>3),but there were no significant differences in vitality between the CASA systems and the inspector with a job certificate(P>0.05).Regarding sperm density detection,when the sperm density was less than 280×106/ml,there were no significant differences between the results displayed by the instruments and the results of the manual hemocytometer counting(P>0.05).[Conclusions]The accuracy of the CASA systems set to uniform parameters was consistent with the accuracy of the visual vitality obtained by an inspector with a job certificate.When the semen was diluted with 3%NaCl solution to a sperm density<280×10^6/ml,the sperm density detected by the CASA systems was consistent in reliability with that obtained by the hemocytometer detection.The CASA systems are faster and more efficient and objective than manual detection,and have the advantages of strong operability and easy promotion.
基金supported by the National Key Research and Development Program of China (2016YFC1201600)Wildlife Borne Infectious Diseases Monitoring Project of the State Forestry Administration (SFA) of China
文摘Hybrid wild boar husbandry is an important component of livestock production in Northeast China.However,the current disease situation of these animals is largely unknown due to a lack of disease surveillance.The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China.Between September 2015 to December 2016,169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin,Inner Mongolia and Heilongjiang provinces.ELISA detected serum antibodies against classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),pseudorabies virus(PRV),porcine circovirus type 2(PCV2)and Japanese encephalitis virus(JEV),but not against African swine fever virus(ASFV),with PCV2 having the highest seropositive rate(87.2–100%in different farms).RT-PCR or PCR performed on the processed samples detected only PCV2,with 33.1%(56/169)of blood samples and 32.8%(20/61)of spleen samples being positive,respectively,indicating widespread PCV2 infection in hybrid wild boars.Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a,PCV2b and PCV2d,with nucleotide and deduced amino acid homologies of 88.5–100%and 88.1–100%,respectively.
基金financed by Wroclaw University of Environmental and Life Sciences,statutory project no.2014
文摘Background: The aim of the study was to analyze the lifetime and culling reasons for boars used in insemination centers(AI centers).Methods: The data collected from 355 culled boars from 1998 to 2013 included: age at start of semen collection,boar herd life, culling reason, daily gain and lean meat content, and number of ejaculates not meeting sales requirements after dilution. Culling reasons were divided into 7 groups: low semen value(LSV), low or lack of libido(LL), leg problems(LP), infectious diseases(ID), old age(OA), reduced demand for semen from the given boar(RD),and others(OT).Results: The most common culling reasons for boars were LSV(23.7%) and RD(22.5%). It was observed that the lowest daily gains were noted in boars culled due to OA. Boars culled due to OA and RD were maintained in production for the longest time(over 1000 d), for LSV and ID retention was about 700 d, and due to LL below 400 d. The survival probability was over 0.9 until 1.5 yr, and just over 0.2 until 4 yr. The highest relative frequency was observed in the 36 ^(th) and 42 ^(nd) mo of life(over 16%). Hazard risk analysis revealed a more than 10 times higher risk of culling in the case of LL, ID or OT, in comparison to OA.Conclusions: The results can be used as a direct point of reference for the identification of emerging problems in AI boar exploitation and the development of an appropriate culling policy in AI centers.
基金Supported by the National Natural Science Foundation of China(31072007)China Agriculture Research System(CARS-36)
文摘Toll-like receptor(TLR)4 plays an important role in the innate immune system and has been involved in resistance/susceptibility to a number of diseases as revealed by studies in human and other domestic animals.Wild boar survives in natural environment without artificial interference and may be different from domestic pig in innate immune system.Here,the complete coding sequence of TLR4 and TLR4A was cloned in wild boar,and two other alternative splicing variants,TLR4B and TLR4C,were obtained.Compared to the counterpart from domestic pig(Gen Bank No.AJ628065),there were five SNPs,c.510T>C,c.960A>G,c.962A>G,c.1605T>G and c.1824A>G,in the coding sequence of wild boar TLR4A gene.TLR4 gene was expressed in all the tissues from wild boar studied with the most abundance in spleen tissue,and m RNA level was significantly lower in spleen from wild boar than that from Min pig.The allele distribution was significantly different at polymorphic loci c.962G>A and c.1027C>A(p<0.01)between wild boar and Min pig.The results would contribute to understand the innate immune system in wild boar.
基金Supported by the National Swine Industry Technology System(CARS-36)the Scientifc and Technological Project of Yangling Demonstration Zone(2014NY-22)
文摘To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents(DiluentⅠ, DiluentⅡ and Diluent Ⅲ) and three boar breed semens(Yorkshire, Landrace and Duroc) were utilized. The sperm motility, effective survival time, survival index, catalase(CAT), the total anti-oxidative capacity(T-AOC) and malondialdehyde(MDA) levels were evaluated. The results showed that there were significant interaction effects between diluents and breeds on the boar sperm motility(P<0.001), survival time(P<0.001), CAT levels(P<0.001) and T-AOC levels(P<0.001), but neither effects nor interaction effects between diluents and breeds on survival index(P>0.05). All of the parameters varied significantly with the increase of the storage time(P<0.001). The survival time increased 12.9% in Yorkshire boar semen diluted with Diluent Ⅲ than with DiluentⅡ, while the survival time increased 6.6% in Landrace boar semen diluted with DiluentⅡ than with Diluent Ⅲ. Both CAT and T-AOC levels were significantly positive correlated with sperm motility in all the three boar breeds(P<0.001), while MDA levels were significantly negative correlated with sperm motility(P<0.001). These results indicated that Diluent Ⅲ and DiluentⅡ were the optimal commercial diluents for Yorkshire and Landrace boar semen stored at 17℃, respectively.
基金supported by the Association for Bioeconomy Research (FBF e.V., Germany)by the Rentenbank-Germany’s development agency for agribusiness+3 种基金by the BLE–Germany’s Federal Office for Agriculture and Food (AMIKOS 28-RZ-3.054, 28-RZ-3.051)by the CAPES and DAAD(57390778PROBAL 88887.185883/2018-00)Open Access funding enabled and organized by Projekt DEAL。
文摘Background: Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility.Objectives: To examine chilling injury and the number and type of bacteria in boar semen stored at 5 ℃ in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions.Material and methods: Boar ejaculates were extended with AndroStar~? Premium, stored at 17 ℃ with and at 5 ℃ without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study.Results: Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher(P < 0.05) up until 72 h in sperm stored at 5 ℃ compared to 17 ℃ but did not differ after 144 h.After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 ℃, bacteria counts were below 103 CFU/mL and the bacteria spectrum was similar to that of raw semen. In 88% of 34 boars,cooled semen fulfilled the requirements for insemination. Fertility was high and did not differ(P > 0.05) between sow groups inseminated with semen stored antibiotic-free at 5 ℃ and semen stored at 17 ℃ with antibiotics.Conclusion: Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable.
文摘Wild boar is one of the most important beast resources.It plays an important role in the maintenance of biological diversity.The genetic resources of wild boar can not only protect the genetic resources,but also improve the formation of new breeds in pigs.This paper summarized the advance on the main biological characteristics of wild boars,evolutionary origin between wild boars and domesticated pigs,and development and utilization of wild boars aimed to provide further insight into wild boar's genetic research and its resource protection.
基金supported by the“Académie de Recherche et d’Enseignement Supérieur(ARES)de la Commission de la Coopération au Développement(CUD)”of Belgium for its support to the realization of this work through Research and Development Project(PRD)titled“Professionalisation of swine value chain actors in the Ouémé and Plateau regions,Benin.”
文摘Objective:To characterize the semen of three genetic types of boars(local,improved and Large White)reared in Benin.Methods:Semen of local,improved and Large White boars reared in Benin were collected using the gloved hand method and analyzed to determine volume,pH,concentration,mobility,motility,and morphology.The effect of the genetic type of boar on semen characteristics was aslo studied.Results:Duration of ejaculation and semen volume of Large White boar were significantly higher than those of local and improved boars(P<0.05).The semen of improved boars had a higher motility score than that of Large White and local boars(P<0.001).The semen of local boars was more concentrated in the spermatozoa than that of improved and Large White boars(P<0.05).The proportion of spermatozoa of improved boars with normal morphology(93.6%)was significantly higher than that of local(82.2%)and Large White boars(81.6%)(P<0.001).The proportion of spermatozoa with folded tails in the semen of Large White boars(9.2%)was significantly higher than that observed in improved(1.8%)and local(5.0%)boars(P<0.001).The proportion of spermatozoa with proximal cytoplasmic droplets in semen of improved boars(2.7%)was significantly lower than that in Large White(6.8%)and local(9.7%)boars(P<0.001).The local(1.5%)and Large White boars(1.1%)showed more spermatozoa with distal cytoplasmic droplets in their semen compared to the improved boars(0.4%).Conclusions:The semen characteristics of pigs reared in Benin vary from one genetic type to another.Each genetic type has a strong point.The Large White boar produces larger semen,the local boar produces more concentrated semen and the improved boar produces spermatozoa that are morphologically better.The semen of these three genetic types can be used in artificial insemination but the improved boar's semen is more recommended.
文摘The identification of superior sire is largely dependent on the semen quality evaluations, currently through computer-aided sperm class analyser (CASA). Immunoassays present a viable method for analysis of proteins associated with fertility, such as osteopontin. We have targeted osteopontin at an upstream epitope at position 249 - 267 amino acids and a downstream epitope at position 59 - 74 amino acids of full osteopontin protein in raw, BTS, Kobidil, Tris and Citrate liquid preserved Large white and Kolbroek boar semen. The Genetool analysis software was used to quantify the amount of osteopontin detected. Three boars per breed were used and experiments were repeated five times. Our results revealed that the downstream 15 amino acid epitope was more sensitive to osteopontin antibody. Between total motility and osteopontin, Kolbroek boar semen preserved in Kobidil, Citrate, BTS and Tris extenders revealed positive correlations of 0.564, 0.471, -0.960 and 0.330, respectively. Large white boars semen showed correlations of -0.043, 0.655, 0.778, and 0.202 Kobidil, Citrate, BTS and Tris, respectively. For rapid motility and osteopontin, Kolbroek boar semen revealed positive correlations of 0.842, 0.601, 0.164 and 0.202, respectively while large white boars semen showed correlations of -0.909, -0.144, -0.210, and 0.089, respectively. Only BTS and Kobidil showed high negative correlations between osteopontin and viability in Kolbroek (-0.975) and Large white (-0.746) boar semen, respectively. No significant correlations with osteopontin were observed when Tris extender was used in both breeds. In conclusion, Kolbroek boar semen requires osteopontin in Kobodil extender while large white boar semen requires osteopontin in BTS extender.
文摘Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of the fatty acid source, level of inclusion and breed of boars tested. Three groups of 87 boars each, from two genetic lines (PIC 337 and PIC 800), were assigned to treatment based on age, mean sperm production (previous 12 weeks), and body condition score. All boars received a corn-soybean meal diet with a commercial fish oil supplement providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/boar/day of docosahexaenoic acid (DHA) as a preconditioning diet. On 10-Aug., 2020, the DHA source was changed to a stabilized starch imbedded source of refined fish oil (Salmate®), providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">test diet. Two additional levels providing 2.38 and 2.94</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA were fed for a 9 week pretreatment period and during the test period. Salmate® fed at 2.38</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA resulted in a reduction in the number of rejected ejaculates (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.045) by 7.5% and 6.4% compared to the lowest and highest inclusion rates, respectively. There w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> no treatment</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> by genetic line interactions. A retrospective study of semen production and quality of 77 boars on the Salmate® diet containing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d DHA was done to compare to the original source of DHA at the same inclusion level. There were no differences in semen quality parameters between the 2 lipid sources. Ejaculate volume increased from 177.9 ml to 233.4 ml (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) and total sperm cells per ejaculate increased from 69.7 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> to 82.0 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) due to substitution of Salmate®. Adding Salmate® at 2.38 g/b/d resulted in a lower number of rejected ejaculates per boar by 7.5% and 6.4% vs. 1.83 and 2.94 g/b/d, respectively, and boars fed Salmate® at 1.83 g/b/d produced 17% more doses than the competing product.
文摘This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.
文摘After the famous“Rainforest Trilogy”such as Monkey Cup and Elephants,Zhang Guixing,a Malaysian Chinese writer in Taiwan,recently published another Nanyang story Boar Crossing the River,in the background of anti-Japanese War,focusing on the massacres and resistance of the Chinese in Krokop,a Malaysian Chinese village in Borneo from 1941 to 1945 according to a list of“Committee for the Relief of Refugees of the Motherland”.In this novel,Zhang Guixing changes the accustomed narrative mode of individual family history,expands the description to the group images of the Chinese in Krokop,highlights their patriotic sacrifice,and saves the reality of the slaughter of the compatriots by magic means such as the myths and legends of Borneo and masks in children’s imagination.It is also noteworthy that the novel talks about the relationship between individual freedom and national identity in depth,and describes the tragic fate of cross-national love in the period of national opposition.These nation-state discourses in Boar Crossing the River are Zhang Guixing’s biggest transitions when missing in the literary world for 17 years,which indicate a landmark progress in his spiritual history.
基金partially supported by the CONACYT(Mexico)grant 0105961/10110/194/09.
文摘Sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane;the acrosome reaction (AR) is an exocytotic event induced by calcium influx, enabling the spermatozoa to penetrate the zona pellucida. These processes can be achieved only if the spermatozoa have good viability;indeed, determination of sperm viability is used for the assessment of semen quality. Membrane integrity and mitochondrial activity are important viability parameters of spermatozoa and fluorescent techniques based on membrane permeability to dyes have been developed to determine these parameters. The aim of this work was to determine the viability of boar sperm (fresh, one hour of capacitation induction and 20 min of AR induction) by flow cytometry using propidium iodide (PI) (1.25 μg/mL) and rhodamine 123 (R123) (0.20 μg/mL). Aliquots of 5 × 105 sperm were incubated with each fluorochrome separately and simultaneously for 10 or 20 min, respectively, at 38℃. The proportion of labeled spermatozoa and their fluorescence intensities were measured using a flow cytometer. The fluorescence index (FI) with PI gradually increased during the incubation and we found significant differences between all the groups. With R123, the FI increased in the capacitated sperm but decreased in the acrosome-reacted sperm, with significant differences between the fresh and capacitated spermatozoa. Our results suggest that the increase in the R123 fluorescence intensity in capacitated spermatozoa is due to changes in the mitochondrial membrane activity because the spermatozoa experienced changes in membrane fluidity and flagellar activation during capacitation. The use of fluorochromes and flow cytometry is a good tool for monitoring many markers of sperm function. Although capacitation and AR processes have been well studied, there is still much information to be elucidated with regard to these complex processes.
基金Funding for this study was provided by National Basic Research Program of China(973 Program,Grant 2014CB138501)the National Transgenic Animal Breeding Grand Project(2014ZX08006–005)the Program for Changjiang Scholars and Innovation Research Teams in the University(IRT_15R62)
文摘Background: Follicle-stimulating hormone(FSH) is a gonadotropin synthesized and secreted by the pituitary gland.FSH stimulates follicle development and maturation in females. It also plays an important role in spermatogenesis in males, including humans and mice. However, the effects of FSH on male pigs are largely unknown. In this study,we generated transgenic pigs to investigate the effects of FSHα/β overexpression on reproductive traits in boars.Results: After five transgenic F_0 founders were crossed with wide-type pigs, 193 F_1 animals were obtained. Of these,96 were confirmed as transgenic. FSHα and FSHβ m RNAs were detected only in pituitary tissue. Transgenic boars exhibited significantly higher levels of FSHα and FSHβ m RNA, serum FSH, and serum testosterone, compared to ful-sib non-transgenic boars. Significant increases in testis weight, vas deferens diameter, seminiferous tubule diameter,and the number of Leydig cel s were observed, suggesting that the exogenous FSHα/β affects reproductive traits. Final y,transgenic and non-transgenic boars had similar growth performance and biochemical profiles.Conclusions: Pituitary-specific overexpression of FSHα/β genes is likely to impact reproductive traits positively, as indicated by enhancements in serum testosterone level, testis weight, the development of vas deferens, seminiferous tubules, and Leydig cells in transgenic boars. A high level of serum FSH induces secretion of serum testosterone,possibly by boosting the number of Leydig cells, which presumably increases the libido and the frequency of sexual activity in transgenic boars. Our study provides a preliminary foundation for the genetic improvement of reproductive traits in male pigs.
基金Supported by Natural Science Foundation of Jiangsu Province(BK2008589)"Blue Project" of Universities in Jiangsu Province[SJS(2010)No.27]Research Cooperation Projects of Wuxi City
文摘To explore the correlation of evaluation methods on boar semen quality,the motility,PI staining,hypotonic swelling test,Rh123 staining and FITC- PNA staining of boar sperm were checked in the study,and its correlation with IVF was statistically calculated. The results showed that various indicators had strong linear correlation with each other,and the correlation degree between acrosome integrity and IVF rate was the highest,indicating that acrosome integrity was superior to other indicators in predicting IVF rate.
