期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichoderma viride endochitinase gene 被引量:1
1
作者 YU Ya ZHANG Lei +8 位作者 LIAN Wei-ran XU Feng-feng LI Shuang-tao XIANG Juan ZHANG Guo-zhen HU Zan-min ZHAO Bing REN Shu-xin GUO Yang-dong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第3期430-437,共8页
A endochitinase gene(Tch) from the fungus Trichoderma viride was introduced into broccoli(Brassica oleracea var. italica) by Agrobacterium-mediated transformation. Sixty-eight putative transformants were obtained ... A endochitinase gene(Tch) from the fungus Trichoderma viride was introduced into broccoli(Brassica oleracea var. italica) by Agrobacterium-mediated transformation. Sixty-eight putative transformants were obtained and the presence of the Tch gene was confirmed by both PCR and Southern blot analysis. RT-PCR analysis showed an accumulation of the transcript encoding the endochitinase protein in the transgenic plants. Using real-time quantitative PCR, the expression profiling of endochitinase gene was analyzed. Primary transformants and selfed progeny were examined for expression of the endochitinase using a fluorometric assay and for their resistance to the pathogenic fungi Botrytis cinerea and Rhizoctonia solani. The endochitinase activities in T0 in vitro plants, T0 mature plants and T1 mature plants were correlated with leaf lesions, and the transgenic line T618 had high endochitinse activities of 102.68, 114.53 and 120.27 nmol L–1 MU min–1 mg–1 protein in the three kinds of plants, respectively. The endochitinase activity showed a positive correlation with the resistance to the pathogens. Most transgenic T0 broccoli had increased resistance to the pathogens of B. cinerea and R. solani in leaf assays and this resistance was confirmed to be inheritable. These findings suggested that expression of the Tch gene from T. viride could enhance resistance to pathogenic fungi in Brassica species. 展开更多
关键词 botrytis cinerea broccoli endochitinase gene genetic transformation rhizoctonia solani
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部