To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlus...To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and brain.展开更多
AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the ...AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the presence of sulfuric acid to determine astragaloside in pig serum. The reaction was allowed to procced on a 60℃ water bath for 20 min. RESULTS The reaction product of astrogaloside and anisic acid has excitation and emission maxima at 320 and 387 nm, respectively. The linear range of determination is from 0 2 to 40 μg·mL -1 . The detection limit is 0 02 μg·mL -1 . The recoveries of samples are between 98 65% and 102 4%. CONCLUSION The proposed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine several kinds of samples successfully.展开更多
基金National Natural Science Foundation of China(Grant No.81373973,81573872,81873228)Science and Technology Planning Project of Guangdong Province(Grant No.2017KZDXM018).
文摘To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction(BYHWD)with different dosages of Astragalus(Yiqi groups and Huoxue groups)applicating in rats with middle cerebral artery occlusion(MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus(3.09,6.17,12.34 g/Kg)and Huoxue goups(2.32 g/Kg)when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were(88.43±3.82,69.18±0.11)min,MRT were(138.56±4.83,113.62±2.42)min,and AUC0-twere(28488.35±4800.32,140614.80±23954.05)ng/mL min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were(82.16±1.78,67.08±3.69)min,and MRT were(127.95±2.70,116.58±4.13),AUC0-t were(48619.25±6745.75,159026.00±15003.33)ng/mL min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were(80.29±1.12,69.69±0.87)min and MRT was(128.79±1.46,118.78±4.56)min AUC0-twere(109942.90±13101.83,189417.90±22311.00)ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and brain.
文摘AIM To develop a new determination method of astrogaloside in anisic acid sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the presence of sulfuric acid to determine astragaloside in pig serum. The reaction was allowed to procced on a 60℃ water bath for 20 min. RESULTS The reaction product of astrogaloside and anisic acid has excitation and emission maxima at 320 and 387 nm, respectively. The linear range of determination is from 0 2 to 40 μg·mL -1 . The detection limit is 0 02 μg·mL -1 . The recoveries of samples are between 98 65% and 102 4%. CONCLUSION The proposed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine several kinds of samples successfully.
