应用免疫组织化学和生物统计分析相结合的方法,对异翅负蝗Atractomorp haheteroptera Bei Bienko配子发生过程中c-kit特异表达特点和动态进行研究。结果表明,（1）精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均...应用免疫组织化学和生物统计分析相结合的方法,对异翅负蝗Atractomorp haheteroptera Bei Bienko配子发生过程中c-kit特异表达特点和动态进行研究。结果表明,（1）精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;（2）卵子发生过程中,第1-6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失;（3）此外,滤泡细胞、输卵管和受精囊的腺细胞中有c-kit蛋白颗粒的存在;（4）从c-kit蛋白季节动态变化看,精子发生和卵子发生中的阳性表达都随着时间的延续出现下降的态势。因此,c-kit蛋白的特异性表达提示该蛋白参与配子发生过程的阶段性调控,具有重要的生理作用。展开更多
. This study investigated the expression of human telomerase reverse transcriptase (hTERT) and c-kit in a cohort of serous ovarian carcinomas by immunohistochemistry with regard to outcome and clinicopathologic variab.... This study investigated the expression of human telomerase reverse transcriptase (hTERT) and c-kit in a cohort of serous ovarian carcinomas by immunohistochemistry with regard to outcome and clinicopathologic variables. Methods. Formalin-fixed, paraffin-embedded archival tissue sections of 10 benign serous cystadenomas, 10 serous neoplasms of low malignant potential (LMP), and 41 serous ovarian carcinomas were immunostained with antibodies to hTERT and c- kit. Immunostaining was scored with regard to quantity and intensity of positively stained cells as negative or weak, moderate, and strong. Mitotic activity was determined as mitotic figures per 10 high power fields. Results. hTERT expression was negative in serous cystadenomas; 70% of LMP showed strong nuclear immunoreactivity. In serous carcinomas, nuclear and sometimes cytoplasmic immunoreactivity was observed; 14% of cases were scored as negative, 42% as moderate, and 44% as strong. hTERT immunoreactivity increased with grade (P < 0.0192) and mitotic activity (P = 0.0018), but not with FIGO stage (P = 0.2752), and was related with outcome (P = 0.0477). No c-kit immunoreactivity was observed in serous cystadenomas and LMP; 27% of serous carcinomas were negative, 46% showed moderate, and 27% strong immunostaining. ckit immunoreactivity was positively correlated with grade (P = 0.0008) and FIGO stage (P = 0.0247), but not with mitotic activity (P = 0.1433) and outcome (P = 0.1145); however, c-kit expression was positively related with poor outcome in FIGO II and III stages (P = 0.0105). Conclusions. hTERT and ckit are frequently up-regulated in serous ovarian carcinomas; c-kit immunoexpression may serve as a marker of aggressive behavior in high stage tumors.展开更多
文摘应用免疫组织化学和生物统计分析相结合的方法,对异翅负蝗Atractomorp haheteroptera Bei Bienko配子发生过程中c-kit特异表达特点和动态进行研究。结果表明,（1）精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;（2）卵子发生过程中,第1-6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失;（3）此外,滤泡细胞、输卵管和受精囊的腺细胞中有c-kit蛋白颗粒的存在;（4）从c-kit蛋白季节动态变化看,精子发生和卵子发生中的阳性表达都随着时间的延续出现下降的态势。因此,c-kit蛋白的特异性表达提示该蛋白参与配子发生过程的阶段性调控,具有重要的生理作用。
文摘. This study investigated the expression of human telomerase reverse transcriptase (hTERT) and c-kit in a cohort of serous ovarian carcinomas by immunohistochemistry with regard to outcome and clinicopathologic variables. Methods. Formalin-fixed, paraffin-embedded archival tissue sections of 10 benign serous cystadenomas, 10 serous neoplasms of low malignant potential (LMP), and 41 serous ovarian carcinomas were immunostained with antibodies to hTERT and c- kit. Immunostaining was scored with regard to quantity and intensity of positively stained cells as negative or weak, moderate, and strong. Mitotic activity was determined as mitotic figures per 10 high power fields. Results. hTERT expression was negative in serous cystadenomas; 70% of LMP showed strong nuclear immunoreactivity. In serous carcinomas, nuclear and sometimes cytoplasmic immunoreactivity was observed; 14% of cases were scored as negative, 42% as moderate, and 44% as strong. hTERT immunoreactivity increased with grade (P < 0.0192) and mitotic activity (P = 0.0018), but not with FIGO stage (P = 0.2752), and was related with outcome (P = 0.0477). No c-kit immunoreactivity was observed in serous cystadenomas and LMP; 27% of serous carcinomas were negative, 46% showed moderate, and 27% strong immunostaining. ckit immunoreactivity was positively correlated with grade (P = 0.0008) and FIGO stage (P = 0.0247), but not with mitotic activity (P = 0.1433) and outcome (P = 0.1145); however, c-kit expression was positively related with poor outcome in FIGO II and III stages (P = 0.0105). Conclusions. hTERT and ckit are frequently up-regulated in serous ovarian carcinomas; c-kit immunoexpression may serve as a marker of aggressive behavior in high stage tumors.
