Changes of 5-hydroxytryptamine(5-HT)and 5-hydroxyindoleacetic acid(5-HIAA)levels in brain cortex and serum were studied using spectrofluorometer follow-ing brain injury in rats.Cyproheptadine,a receptor antagonist of ...Changes of 5-hydroxytryptamine(5-HT)and 5-hydroxyindoleacetic acid(5-HIAA)levels in brain cortex and serum were studied using spectrofluorometer follow-ing brain injury in rats.Cyproheptadine,a receptor antagonist of 5-HT,was employedand its effect on alterations of 5-HT and 5-HIAA contents in brain cortex and serum af-ter brain injury was investigated.The results showed that after brain injury levels of5-HT and 5-HIAA in the brain cortex increased markedly,The increase of 5-HT in thebrain cortex reached its peak at 48h after brain injury,and its value was 4.29±0.44μg/g,which was 4.8 times of control value.The increase of 5-HIAA in the brain cortexreached its peak at 24h after brain injury,and its value was 5.48±0.41μg/g,which was5.8times of control value.The change of 5-HT in the serum was not significant,but thelevel of 5-HIAA in the serum was increased significantly.Cyproheptadine could reducelevels of 5-HT and 5-HIAA in this experiment.The value of 5-HT in the brain cortexdecreased to 1.32±0.09μg/g,and the value of 5-HIAA decreased to 1.36±0.10μg/g.Thelevel of 5-HIAA in the serum was also reduced significantly.It was 0.27±0.03μg/ml.展开更多
Experiments were performed on 25 rats. Putative serotonergic antagonist, Cyproheptadine(Cyp), was applied microiontophoretically on the nucleus parafascicularis of the thalamus (Pf) andthe effect of Cyp on Pf nocicept...Experiments were performed on 25 rats. Putative serotonergic antagonist, Cyproheptadine(Cyp), was applied microiontophoretically on the nucleus parafascicularis of the thalamus (Pf) andthe effect of Cyp on Pf nociceptive neuronal discharge was observed. The result suggested that Cypcan increase the spontaneous and evoked discharge rates of Pf nociceptive neuron, and that Cypmay antagonize the inhibitory effect of endogenous serotonin on the spontaneous and evokeddischarges of Pf nociceptive neurons.展开更多
Like electroacupuncture (EA),electrical stimulation of the dorsal raphe nucle-us (DRN)also inhibits the cAMP induced cortical epileptic waves and the EA inducedinhibition can be abolished or markedly reduced by iv cyp...Like electroacupuncture (EA),electrical stimulation of the dorsal raphe nucle-us (DRN)also inhibits the cAMP induced cortical epileptic waves and the EA inducedinhibition can be abolished or markedly reduced by iv cyproheptadine (CYP) or destruc-tion of DRN by electrolysis.The results suggest that 5-HT neurons in the DRN mayplay an important role in the EA inhibition of the generation of cAMP induced elec-trocorticogram (ECoG) epileptic waves.展开更多
BACKGROUND Serotonin syndrome(SS)is an underdiagnosed drug-induced clinical syndrome resulting from the excess intrasynaptic concentration of serotonin.Very limited information is available about chronic SS.AIM To eva...BACKGROUND Serotonin syndrome(SS)is an underdiagnosed drug-induced clinical syndrome resulting from the excess intrasynaptic concentration of serotonin.Very limited information is available about chronic SS.AIM To evaluate the epidemiological,clinical,and other aspects of the insidious onset SS.METHODS We retrospectively evaluated 14 consecutive adult patients(>18 years)who had complaints for more than 6 wk at the time of consultation and met the Hunter criteria for SS.RESULTS The mean age was 41.1 years(range:21-61 years),with a male preponderance(64%).Although tremors were observed in all patients,this was a presenting complaint in only 43%of patients.Generalized body pain,insomnia,and restlessness were common presenting features(50%each).Other common clinical features were stiffness of the limbs(43%),diaphoresis(43%),gait disturbances(36%),bowel disturbances(36%),dizziness(29%),sexual dysfunctions(21%),incoordination(14%),and fatigue(14%)The mean duration of symptoms before the diagnosis of SS was 13.5±5.8 wk(range:6-24 wk).Amitriptyline was the most common drug(n=6,43%),followed by tramadol(n=5,36%)and sodium valproate(n=5,36%).All patients received cyproheptadine,a 5-hydroxytryptamine2A antagonist,as treatment and noted an excellent response CONCLUSION This study represents the largest study on chronic SS.We suggest that patients receiving serotonergic drugs should be physically examined for the presence of SS upon the development of new symptoms.展开更多
文摘Changes of 5-hydroxytryptamine(5-HT)and 5-hydroxyindoleacetic acid(5-HIAA)levels in brain cortex and serum were studied using spectrofluorometer follow-ing brain injury in rats.Cyproheptadine,a receptor antagonist of 5-HT,was employedand its effect on alterations of 5-HT and 5-HIAA contents in brain cortex and serum af-ter brain injury was investigated.The results showed that after brain injury levels of5-HT and 5-HIAA in the brain cortex increased markedly,The increase of 5-HT in thebrain cortex reached its peak at 48h after brain injury,and its value was 4.29±0.44μg/g,which was 4.8 times of control value.The increase of 5-HIAA in the brain cortexreached its peak at 24h after brain injury,and its value was 5.48±0.41μg/g,which was5.8times of control value.The change of 5-HT in the serum was not significant,but thelevel of 5-HIAA in the serum was increased significantly.Cyproheptadine could reducelevels of 5-HT and 5-HIAA in this experiment.The value of 5-HT in the brain cortexdecreased to 1.32±0.09μg/g,and the value of 5-HIAA decreased to 1.36±0.10μg/g.Thelevel of 5-HIAA in the serum was also reduced significantly.It was 0.27±0.03μg/ml.
文摘Experiments were performed on 25 rats. Putative serotonergic antagonist, Cyproheptadine(Cyp), was applied microiontophoretically on the nucleus parafascicularis of the thalamus (Pf) andthe effect of Cyp on Pf nociceptive neuronal discharge was observed. The result suggested that Cypcan increase the spontaneous and evoked discharge rates of Pf nociceptive neuron, and that Cypmay antagonize the inhibitory effect of endogenous serotonin on the spontaneous and evokeddischarges of Pf nociceptive neurons.
文摘Like electroacupuncture (EA),electrical stimulation of the dorsal raphe nucle-us (DRN)also inhibits the cAMP induced cortical epileptic waves and the EA inducedinhibition can be abolished or markedly reduced by iv cyproheptadine (CYP) or destruc-tion of DRN by electrolysis.The results suggest that 5-HT neurons in the DRN mayplay an important role in the EA inhibition of the generation of cAMP induced elec-trocorticogram (ECoG) epileptic waves.
文摘BACKGROUND Serotonin syndrome(SS)is an underdiagnosed drug-induced clinical syndrome resulting from the excess intrasynaptic concentration of serotonin.Very limited information is available about chronic SS.AIM To evaluate the epidemiological,clinical,and other aspects of the insidious onset SS.METHODS We retrospectively evaluated 14 consecutive adult patients(>18 years)who had complaints for more than 6 wk at the time of consultation and met the Hunter criteria for SS.RESULTS The mean age was 41.1 years(range:21-61 years),with a male preponderance(64%).Although tremors were observed in all patients,this was a presenting complaint in only 43%of patients.Generalized body pain,insomnia,and restlessness were common presenting features(50%each).Other common clinical features were stiffness of the limbs(43%),diaphoresis(43%),gait disturbances(36%),bowel disturbances(36%),dizziness(29%),sexual dysfunctions(21%),incoordination(14%),and fatigue(14%)The mean duration of symptoms before the diagnosis of SS was 13.5±5.8 wk(range:6-24 wk).Amitriptyline was the most common drug(n=6,43%),followed by tramadol(n=5,36%)and sodium valproate(n=5,36%).All patients received cyproheptadine,a 5-hydroxytryptamine2A antagonist,as treatment and noted an excellent response CONCLUSION This study represents the largest study on chronic SS.We suggest that patients receiving serotonergic drugs should be physically examined for the presence of SS upon the development of new symptoms.
