Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of ...Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.展开更多
[Objectives]To investigate the acute toxicity of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract to mice.[Methods]The medium lethal dose(LD50)in mice could not be determined,so the ma...[Objectives]To investigate the acute toxicity of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract to mice.[Methods]The medium lethal dose(LD50)in mice could not be determined,so the maximum dose method was used for experiment.Mice were not fed but allowed to drink water for 16 h before the experiment.There were 74 SPF Kunming Mice,half male and half female,and it was divided into extract group and blank control group.The toxicity and death were recorded after intragastric administration and regular close observation.If any mice died,the dead mice were dissected.The changes of heart,liver,spleen,lung,kidney,brain,stomach,small intestine and other organs were observed with the naked eye.At the end of the 14 d observation period,the surviving mice in each group were killed and dissected.The gross pathological changes of the main organs were observed with the naked eye according to the same method mentioned above.[Results]There were no obvious toxic symptoms and no death in the mice.[Conclusions]The maximum doses of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract in mice were 1.61,1.71,1.65 and 1.79 g/L,respectively.Thus it was found that the quarterly tests of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract suggested that the medicine was safe.展开更多
[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extr...[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extraction method,and Soxhlet extraction method were used to extract total alkaloids,and the total alkaloids of Abrus Cantoniensis Hance were measured at 238 nm wavelength. [Results] The extraction rate of ethanol extraction method was 14. 76 mg/100 g; the extraction rate of ultrasonic extraction method was 56. 85 mg/100 g; the extraction rate of acid water extraction method was 56. 63 mg/100 g; the extraction rate of Soxhlet extraction method was 58. 19 mg/100 g.[Conclusions]Results indicate that Soxhlet extraction method can obtain the highest extraction rate.展开更多
Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medic...Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups:negative control group[with Mueller-Hinton broth(MHB)],positive control group(with 75%ethanol),blank group(with MHB)and test group(with the ethyl acetate part of Abrus cantoniensis).The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration(MIC).Time-kill curve experiments,scanning electron microscopy,the content of DNA,RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S.aureus.The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University(approval No.XJTULAC2016-412)on January 22,2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S.aureus with an inhibition zone diameter of 16.4mm and MIC value of 0.5mg/mL.The general activity range of the ethyl acetate part,determined using a time-killing curve,was found to be 0.5mg/mL to 40mg/mL(MIC to 80MIC).Changes in the scanning electron microscopy images and of DNA,RNA and proteins of S.aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures,which results in protoplasm leakage,and eventually bacterial cell death.展开更多
基金This work was supported by National Natural Science Foundation of China(No.81360684)Guangxi Key Research and Development Plan Project(Gui Ke AB18221095)+3 种基金Teaching Teacher Training Project from Youjiang Medical University for Nationalities-National Teaching Teacher Training Project(You Hospital Zi[2018]No.98)High-level Talent Research Projects from Youjiang Medical University For Nationalities(No.01002018079)China National and Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599022)China Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599073).
文摘Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.
基金Supported by Construction Project of China State Administration of Traditional Chinese Medicine on Key Discipline of Traditional Chinese Medicine—Chemistry of Traditional Chinese Medicine during the"Twelfth Five-Year Plan"Period[Guo Zhong Yi Yao Ren Jiao Fa(2012)32]Key R&D Project in Guangxi,China(GuiKe AB18221095)+1 种基金Guangxi Natural Science Foundation of China(2017GXNSFAA198031)2017 College Students’Innovation and Entrepreneurship Training Program in Guangxi Zhuang Autonomous Region,China(201710599055).
文摘[Objectives]To investigate the acute toxicity of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract to mice.[Methods]The medium lethal dose(LD50)in mice could not be determined,so the maximum dose method was used for experiment.Mice were not fed but allowed to drink water for 16 h before the experiment.There were 74 SPF Kunming Mice,half male and half female,and it was divided into extract group and blank control group.The toxicity and death were recorded after intragastric administration and regular close observation.If any mice died,the dead mice were dissected.The changes of heart,liver,spleen,lung,kidney,brain,stomach,small intestine and other organs were observed with the naked eye.At the end of the 14 d observation period,the surviving mice in each group were killed and dissected.The gross pathological changes of the main organs were observed with the naked eye according to the same method mentioned above.[Results]There were no obvious toxic symptoms and no death in the mice.[Conclusions]The maximum doses of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract in mice were 1.61,1.71,1.65 and 1.79 g/L,respectively.Thus it was found that the quarterly tests of petroleum ether extract,n-butanol extract,ethyl acetate extract and water extract suggested that the medicine was safe.
基金Supported by the 12th Five-Year TCM Key Discipline Chinese Medicine Chemistry Construction Program of State Administration of Traditional Chinese Medicine,China(Guo Zhong Yi Yao Ren Jiao Fa[2012]32)Natural Science Foundation Project of Guangxi(2013GXNSFAA019240)+2 种基金Key Discipline Chinese Medicine Chemistry Construction Program of Guangxi,China(Gui Jiao Ke Yan[2013]16)Student’s Platform for Innovation and Entrepreneurship Training Program of Guangxi in 2015(201510599037)Innovation and Entrepreneurship Ethnical Medicine Teaching Team Program of Guangxi Zhuang Autonomous Region,China(Gui Jiao Gao Jiao[2015]93 & Gui Jiao Gao Jiao[2016]6)
文摘[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extraction method,and Soxhlet extraction method were used to extract total alkaloids,and the total alkaloids of Abrus Cantoniensis Hance were measured at 238 nm wavelength. [Results] The extraction rate of ethanol extraction method was 14. 76 mg/100 g; the extraction rate of ultrasonic extraction method was 56. 85 mg/100 g; the extraction rate of acid water extraction method was 56. 63 mg/100 g; the extraction rate of Soxhlet extraction method was 58. 19 mg/100 g.[Conclusions]Results indicate that Soxhlet extraction method can obtain the highest extraction rate.
基金the National Key Research&Development Program of China,No.2018YFC0910601(to XX)the National Natural Science Foundation of China,Nos.81701853(to QZ)and 62007026(to HX)the Natural Science Basic Research Plan in Shaanxi Province of China,Nos.2019JQ-519(to XX)and 2019JQ-201(to HX).
文摘Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups:negative control group[with Mueller-Hinton broth(MHB)],positive control group(with 75%ethanol),blank group(with MHB)and test group(with the ethyl acetate part of Abrus cantoniensis).The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration(MIC).Time-kill curve experiments,scanning electron microscopy,the content of DNA,RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S.aureus.The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University(approval No.XJTULAC2016-412)on January 22,2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S.aureus with an inhibition zone diameter of 16.4mm and MIC value of 0.5mg/mL.The general activity range of the ethyl acetate part,determined using a time-killing curve,was found to be 0.5mg/mL to 40mg/mL(MIC to 80MIC).Changes in the scanning electron microscopy images and of DNA,RNA and proteins of S.aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures,which results in protoplasm leakage,and eventually bacterial cell death.
