The recovery of caproic acid from the aqueous stream is of practical relevance due to its market value and the stringent environmental standards. The profound success of reactive extraction has been noted in the recov...The recovery of caproic acid from the aqueous stream is of practical relevance due to its market value and the stringent environmental standards. The profound success of reactive extraction has been noted in the recovery of carboxylic acids from dilute solutions. In present paper, equilibrium of caproic acid with aliquat-336, a quaternary amine compound, in xylene and Methyl Isobutyl Ketone (MIBK) has been studied. Equilibrium parameters such as distribution coefficients, loading ratio, degree of extraction and equilibrium complexation constants have been presented. The data obtained is useful in understanding the equilibrium characteristics and efficient design of the recovery process of caproic acid by reactive extraction.展开更多
BACKGROUND Intrahepatic cholestasis of pregnancy(ICP)is a pregnancy-specific liver condition that typically arises in the middle and late stages of pregnancy.Short-chain fatty acids(SCFAs),prominent metabolites of the...BACKGROUND Intrahepatic cholestasis of pregnancy(ICP)is a pregnancy-specific liver condition that typically arises in the middle and late stages of pregnancy.Short-chain fatty acids(SCFAs),prominent metabolites of the gut microbiota,have significant connections with various pregnancy complications,and some SCFAs hold potential for treating such complications.However,the metabolic profile of SCFAs in patients with ICP remains unclear.AIM To investigate the metabolic profiles and differences in SCFAs present in the maternal and cord blood of patients with ICP and determine the clinical significance of these findings.METHODS Maternal serum and cord blood samples were collected from both patients with ICP(ICP group)and normal pregnant women(NP group).Targeted metabolomics was used to assess the SCFA levels in these samples.RESULTS Significant differences in maternal SCFAs were observed between the ICP and NP groups.Most SCFAs exhibited a consistent declining trend in cord blood samples from the ICP group,mirroring the pattern seen in maternal serum.Correlation analysis revealed a positive correlation between maternal serum SCFAs and cord blood SCFAs[r(Pearson)=0.88,P=7.93e-95].In both maternal serum and cord blood,acetic and caproic acids were identified as key metabolites contributing to the differences in SCFAs between the two groups(variable importance for the projection>1).Receiver operating characteristic analysis demonstrated that multiple SCFAs in maternal blood have excellent diagnostic capabilities for ICP,with caproic acid exhibiting the highest diagnostic efficacy(area under the curve=0.97).CONCLUSION Compared with the NP group,significant alterations were observed in the SCFAs of maternal serum and cord blood in the ICP group,although they displayed distinct patterns of change.Furthermore,the SCFA levels in maternal serum and cord blood were significantly positively correlated.Notably,certain maternal serum SCFAs,specifically caproic and acetic acids,demonstrated excellent diagnostic efficiency for ICP.展开更多
浓香型白酒酿造过程中离不开窖泥中微生物的相互作用,本项目利用宏基因组高通量测序监测人工窖泥中微生物变化。该窖泥采用优质塘泥培养,通过两轮(各三个排次)的发酵产酒,发现窖泥己酸含量从3.86 g/L下降到1.33 g/L,基酒中己酸乙酯含量...浓香型白酒酿造过程中离不开窖泥中微生物的相互作用,本项目利用宏基因组高通量测序监测人工窖泥中微生物变化。该窖泥采用优质塘泥培养,通过两轮(各三个排次)的发酵产酒,发现窖泥己酸含量从3.86 g/L下降到1.33 g/L,基酒中己酸乙酯含量降低60%左右,同时,乳酸乙酯含量升高85%以上;窖泥取样提取宏基因组高通量测序,通过Illumina Miseq对16s r DNA V3+V4区进行测序分析,未发酵窖泥中含有大量的Fimicutes(厚壁菌门)占据优势地位,发酵49 d菌群比例占比较均衡,发酵两轮后菌群比例变化较大,发酵两轮后由于大量的Lactobacillus(耐酸乳酸杆菌)繁殖以及Clostridium(梭菌属)等产己酸微生物含量的降低,抑制了产己酸微生物的生长,导致乳酸大量堆积,窖池pH降低,窖泥板结,碳酸盐等堆积造成窖池老化,可以通过人工老窖泥菌群变化监测判断窖泥质量。展开更多
浓香型白酒的主体香味物质是己酸乙酯,己酸菌是重要的功能菌种。该研究从优质窖泥中分离获得了高产己酸菌株,命名为JZZ,该菌是革兰氏阳性杆菌,经形态学和16S r DNA序列鉴定,属于克氏梭菌(Clostridium kluyveri)。通过培养条件考察,该己...浓香型白酒的主体香味物质是己酸乙酯,己酸菌是重要的功能菌种。该研究从优质窖泥中分离获得了高产己酸菌株,命名为JZZ,该菌是革兰氏阳性杆菌,经形态学和16S r DNA序列鉴定,属于克氏梭菌(Clostridium kluyveri)。通过培养条件考察,该己酸菌最适培养条件为接种量5%,装样量90%,培养温度37℃,p H值为6.5。在最适培养条件下,菌株JZZ的己酸产量均可达到4.36 mg/m L。展开更多
文摘The recovery of caproic acid from the aqueous stream is of practical relevance due to its market value and the stringent environmental standards. The profound success of reactive extraction has been noted in the recovery of carboxylic acids from dilute solutions. In present paper, equilibrium of caproic acid with aliquat-336, a quaternary amine compound, in xylene and Methyl Isobutyl Ketone (MIBK) has been studied. Equilibrium parameters such as distribution coefficients, loading ratio, degree of extraction and equilibrium complexation constants have been presented. The data obtained is useful in understanding the equilibrium characteristics and efficient design of the recovery process of caproic acid by reactive extraction.
基金Supported by The Medical and Health Research Project of Zhejiang Province,No.2023KY1105the Traditional Chinese Medical and Health Research Project of Zhejiang Province,No.2022ZB328.
文摘BACKGROUND Intrahepatic cholestasis of pregnancy(ICP)is a pregnancy-specific liver condition that typically arises in the middle and late stages of pregnancy.Short-chain fatty acids(SCFAs),prominent metabolites of the gut microbiota,have significant connections with various pregnancy complications,and some SCFAs hold potential for treating such complications.However,the metabolic profile of SCFAs in patients with ICP remains unclear.AIM To investigate the metabolic profiles and differences in SCFAs present in the maternal and cord blood of patients with ICP and determine the clinical significance of these findings.METHODS Maternal serum and cord blood samples were collected from both patients with ICP(ICP group)and normal pregnant women(NP group).Targeted metabolomics was used to assess the SCFA levels in these samples.RESULTS Significant differences in maternal SCFAs were observed between the ICP and NP groups.Most SCFAs exhibited a consistent declining trend in cord blood samples from the ICP group,mirroring the pattern seen in maternal serum.Correlation analysis revealed a positive correlation between maternal serum SCFAs and cord blood SCFAs[r(Pearson)=0.88,P=7.93e-95].In both maternal serum and cord blood,acetic and caproic acids were identified as key metabolites contributing to the differences in SCFAs between the two groups(variable importance for the projection>1).Receiver operating characteristic analysis demonstrated that multiple SCFAs in maternal blood have excellent diagnostic capabilities for ICP,with caproic acid exhibiting the highest diagnostic efficacy(area under the curve=0.97).CONCLUSION Compared with the NP group,significant alterations were observed in the SCFAs of maternal serum and cord blood in the ICP group,although they displayed distinct patterns of change.Furthermore,the SCFA levels in maternal serum and cord blood were significantly positively correlated.Notably,certain maternal serum SCFAs,specifically caproic and acetic acids,demonstrated excellent diagnostic efficiency for ICP.
文摘浓香型白酒酿造过程中离不开窖泥中微生物的相互作用,本项目利用宏基因组高通量测序监测人工窖泥中微生物变化。该窖泥采用优质塘泥培养,通过两轮(各三个排次)的发酵产酒,发现窖泥己酸含量从3.86 g/L下降到1.33 g/L,基酒中己酸乙酯含量降低60%左右,同时,乳酸乙酯含量升高85%以上;窖泥取样提取宏基因组高通量测序,通过Illumina Miseq对16s r DNA V3+V4区进行测序分析,未发酵窖泥中含有大量的Fimicutes(厚壁菌门)占据优势地位,发酵49 d菌群比例占比较均衡,发酵两轮后菌群比例变化较大,发酵两轮后由于大量的Lactobacillus(耐酸乳酸杆菌)繁殖以及Clostridium(梭菌属)等产己酸微生物含量的降低,抑制了产己酸微生物的生长,导致乳酸大量堆积,窖池pH降低,窖泥板结,碳酸盐等堆积造成窖池老化,可以通过人工老窖泥菌群变化监测判断窖泥质量。
