The online car-hailing industry, which provides the right of use, has a certain impact on the traditional automobile market, but there is no unified theory on whether it has a positive impact or a negative impact. Bas...The online car-hailing industry, which provides the right of use, has a certain impact on the traditional automobile market, but there is no unified theory on whether it has a positive impact or a negative impact. Based on 362 consumer questionnaire data, this study builds a structural equation model to discuss the driving factors of residents’ choice of online car-hailing and whether the development of online car-hailing will have a certain substitution impact on the sales of private cars. From the perspective of consumers’ purchase intention, the research results show that consumers’ price consciousness, convenience consciousness, environmental protection consciousness and possession tendency will affect their choice of travel mode, and the use of online car-hailing is positively correlated with consumers’ willingness to replace private car ownership with online car-hailing.展开更多
目的分离纯化碧根果致敏原Car i 1,并对其结构进行表征鉴定。方法以新鲜碧根果果仁为原料,通过粉碎、脱脂、浸提、粗分级、凝胶过滤层析,对碧根果致敏原蛋白Car i 1进行分离纯化。结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、液相色谱-串...目的分离纯化碧根果致敏原Car i 1,并对其结构进行表征鉴定。方法以新鲜碧根果果仁为原料,通过粉碎、脱脂、浸提、粗分级、凝胶过滤层析,对碧根果致敏原蛋白Car i 1进行分离纯化。结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、液相色谱-串联质谱法和免疫印迹法3种方法对Cari1进行鉴定,并通过圆二色谱仪与紫外分光光度计表征其二、三级结构。结果本方法纯化获得碧根果致敏原Cari1,单轮制备量可达5 mg以上,且纯度大于95%,蛋白质高级结构未被破坏,能够被全部3名碧根果过敏患者的血清准确识别。结论该纯化方法技术路线简单、设备要求低且单次制备量高,总得率可达65%,操作便捷,为碧根果致敏原Car i 1的相关研究奠定了物质基础。展开更多
2024年3月6日,浙江大学药学院顾臻教授、李洪军研究员联合浙江大学医学院附属第一医院赵鹏主任医师团队在《自然·材料》(Nature Materials)上发表了最新研究成果“Lyophilized lymph nodes for improved delivery of chimeric anti...2024年3月6日,浙江大学药学院顾臻教授、李洪军研究员联合浙江大学医学院附属第一医院赵鹏主任医师团队在《自然·材料》(Nature Materials)上发表了最新研究成果“Lyophilized lymph nodes for improved delivery of chimeric antigen receptor T cells”(DOI:10.1038/s41563-024-01825-z)。该研究提出一种肌体组织工程化递药策略,使用冷冻干燥技术保留患者来源淋巴结的基质结构和关键免疫调节因子存储和递送CAR-T细胞,增强CAR-T细胞的杀伤活性和持久性。展开更多
儿童肿瘤包括血液系统恶性肿瘤、中枢神经系统肿瘤和颅外实体肿瘤。在过去40年中,通过多种疗法,儿童肿瘤的治疗取得了巨大进步[1-2]。然而,在部分复发难治肉瘤、高危神经母细胞瘤及中枢系统肿瘤中,无有效治愈手段,预后差。嵌合抗原受体...儿童肿瘤包括血液系统恶性肿瘤、中枢神经系统肿瘤和颅外实体肿瘤。在过去40年中,通过多种疗法,儿童肿瘤的治疗取得了巨大进步[1-2]。然而,在部分复发难治肉瘤、高危神经母细胞瘤及中枢系统肿瘤中,无有效治愈手段,预后差。嵌合抗原受体T细胞(chimeric antigen receptor T cells,CAR-T)治疗是通过体外改造T细胞,使其能特异性识别和杀伤肿瘤细胞,从而成为治疗肿瘤的一种新技术。目前,CAR-T治疗相关的临床试验正在广泛开展,并且在血液系统恶性肿瘤方面取得令人鼓舞的结果,CAR-T疗法也被尝试用于治疗各种实体肿瘤。然而,由于实体肿瘤特殊的肿瘤异质性和免疫抑制性微环境等问题,CAR-T细胞疗法在实体瘤中的应用仍面临重大挑战[3]。展开更多
Rolling stock manufacturers are finding structural solutions to reduce power required by the vehicles,and the lightweight design of the car body represents a possible solution.Optimization processes and innovative mat...Rolling stock manufacturers are finding structural solutions to reduce power required by the vehicles,and the lightweight design of the car body represents a possible solution.Optimization processes and innovative materials can be combined in order to achieve this goal.In this framework,we propose the redesign and optimization process of the car body roof for a light rail vehicle,introducing a sandwich structure.Bonded joint was used as a fastening system.The project was carried out on a single car of a modern tram platform.This preliminary numerical work was developed in two main steps:redesign of the car body structure and optimization of the innovated system.Objective of the process was the mass reduction of the whole metallic structure,while the constraint condition was imposed on the first frequency of vibration of the system.The effect of introducing a sandwich panel within the roof assembly was evaluated,focusing on the mechanical and dynamic performances of the whole car body.A mass saving of 63%on the optimized components was achieved,corresponding to a 7.6%if compared to the complete car body shell.In addition,a positive increasing of 17.7%on the first frequency of vibration was observed.Encouraging results have been achieved in terms of weight reduction and mechanical behaviour of the innovated car body.展开更多
文摘The online car-hailing industry, which provides the right of use, has a certain impact on the traditional automobile market, but there is no unified theory on whether it has a positive impact or a negative impact. Based on 362 consumer questionnaire data, this study builds a structural equation model to discuss the driving factors of residents’ choice of online car-hailing and whether the development of online car-hailing will have a certain substitution impact on the sales of private cars. From the perspective of consumers’ purchase intention, the research results show that consumers’ price consciousness, convenience consciousness, environmental protection consciousness and possession tendency will affect their choice of travel mode, and the use of online car-hailing is positively correlated with consumers’ willingness to replace private car ownership with online car-hailing.
文摘目的分离纯化碧根果致敏原Car i 1,并对其结构进行表征鉴定。方法以新鲜碧根果果仁为原料,通过粉碎、脱脂、浸提、粗分级、凝胶过滤层析,对碧根果致敏原蛋白Car i 1进行分离纯化。结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、液相色谱-串联质谱法和免疫印迹法3种方法对Cari1进行鉴定,并通过圆二色谱仪与紫外分光光度计表征其二、三级结构。结果本方法纯化获得碧根果致敏原Cari1,单轮制备量可达5 mg以上,且纯度大于95%,蛋白质高级结构未被破坏,能够被全部3名碧根果过敏患者的血清准确识别。结论该纯化方法技术路线简单、设备要求低且单次制备量高,总得率可达65%,操作便捷,为碧根果致敏原Car i 1的相关研究奠定了物质基础。
文摘2024年3月6日,浙江大学药学院顾臻教授、李洪军研究员联合浙江大学医学院附属第一医院赵鹏主任医师团队在《自然·材料》(Nature Materials)上发表了最新研究成果“Lyophilized lymph nodes for improved delivery of chimeric antigen receptor T cells”(DOI:10.1038/s41563-024-01825-z)。该研究提出一种肌体组织工程化递药策略,使用冷冻干燥技术保留患者来源淋巴结的基质结构和关键免疫调节因子存储和递送CAR-T细胞,增强CAR-T细胞的杀伤活性和持久性。
文摘儿童肿瘤包括血液系统恶性肿瘤、中枢神经系统肿瘤和颅外实体肿瘤。在过去40年中,通过多种疗法,儿童肿瘤的治疗取得了巨大进步[1-2]。然而,在部分复发难治肉瘤、高危神经母细胞瘤及中枢系统肿瘤中,无有效治愈手段,预后差。嵌合抗原受体T细胞(chimeric antigen receptor T cells,CAR-T)治疗是通过体外改造T细胞,使其能特异性识别和杀伤肿瘤细胞,从而成为治疗肿瘤的一种新技术。目前,CAR-T治疗相关的临床试验正在广泛开展,并且在血液系统恶性肿瘤方面取得令人鼓舞的结果,CAR-T疗法也被尝试用于治疗各种实体肿瘤。然而,由于实体肿瘤特殊的肿瘤异质性和免疫抑制性微环境等问题,CAR-T细胞疗法在实体瘤中的应用仍面临重大挑战[3]。
文摘Rolling stock manufacturers are finding structural solutions to reduce power required by the vehicles,and the lightweight design of the car body represents a possible solution.Optimization processes and innovative materials can be combined in order to achieve this goal.In this framework,we propose the redesign and optimization process of the car body roof for a light rail vehicle,introducing a sandwich structure.Bonded joint was used as a fastening system.The project was carried out on a single car of a modern tram platform.This preliminary numerical work was developed in two main steps:redesign of the car body structure and optimization of the innovated system.Objective of the process was the mass reduction of the whole metallic structure,while the constraint condition was imposed on the first frequency of vibration of the system.The effect of introducing a sandwich panel within the roof assembly was evaluated,focusing on the mechanical and dynamic performances of the whole car body.A mass saving of 63%on the optimized components was achieved,corresponding to a 7.6%if compared to the complete car body shell.In addition,a positive increasing of 17.7%on the first frequency of vibration was observed.Encouraging results have been achieved in terms of weight reduction and mechanical behaviour of the innovated car body.
