Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit ...Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration.However,the regulatory network of C.humilis in anthocyanin biosynthesis is still unclear.In this study,the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species.Yeast one-hybrid,dual-luciferase assays,and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C.humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS(MYB-binding elements).ChMYB1 interacted with ChbHLH42and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT.In addition,abscisic acid(ABA)treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C.humilis fruit.Interestingly,ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42.Furthermore,ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42.Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C.humilis fruit,deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops.展开更多
Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(NSF))were obtained from Chinese dwarf cherry(Cerasus humilis)fruits.All of them were branched low ...Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(NSF))were obtained from Chinese dwarf cherry(Cerasus humilis)fruits.All of them were branched low methoxylated pectins with an amorphous or partially nanocrystalline nature and eight neutral monosaccharides(arabinose and galactose were most abundant).WSF,CSF and NSF had a degree of methylation(DM)of 35.82%,14.85%and 7.13%,uronic acid(UA)content of 76.02%,83.71%and 69.01%,and total protein content of 2.4%,2.1%and 8.8%,respectively.Their molecular weights were 340.31,330.16 and 141.31 kg/mol,respectively(analyzed by gel permeation chromatography(GPC)).WSF,CSF and NSF exhibited good rheological,thermal,emulsifying,emulsion-stabilizing,water-adsorbing,oil-binding,cholesterol-binding and antioxidant properties.NSF had the highest emulsifying,emulsion stabilizing,water-/oil-/cholesterol-binding and antioxidant capacities,followed by CSF.NSF had the highest viscosity(406.77 m Pa·s),flowability,and resistance to heat-induced changes/damage,which may be related to its lowest polydispersity index,DM and UA content and highest protein content.The three pectin fractions with desirable characteristics can be used as food additives/ingredients and dietary supplements.展开更多
Cerasus humilis is a woody shrub with increasing economic importance. Due to its high oil content in seed with desired fatty acid(FA) composition, C. humilis has been considered as a potential woody oilseed crop in Ch...Cerasus humilis is a woody shrub with increasing economic importance. Due to its high oil content in seed with desired fatty acid(FA) composition, C. humilis has been considered as a potential woody oilseed crop in China. However, FAs accumulation and related molecular mechanisms of FA biosynthesis in C. humilis seed have not been elucidated well. In this study, oil contents and FA compositions of developing C. humilis seed were analyzed. The total oil content in mature seed reached 48.7% while unsaturated FA concentration reached a high level of 96.2%. Three c DNA libraries of C. humilis developing seed were constructed at the beginning and 2 fast oil-accumulation stages, followed by Illumina sequencing with the platform of HiSeq^TM 2000. Differentially expressed unigenes(DEGs) were identified during the respective seed development stages to investigate transcription dynamics. Among DEGs, 82 unigenes were identified as being closely involved in de novo FA and triacylglycerol(TAG) biosynthesis. Surprisingly, among DEGs involved in TAG biosynthesis, expression of unigenes encoding GPATs(glycerol-3-phosphate acyltransferase) were relatively low and unigenes encoding LPAATs(lysophosphatidic acid acyltransferase) were highly expressed, suggesting that LPAAT contributed more in storage lipids metabolism in C. humilis seed. Genes encoding DGAT2(diacylgycerol acyltransferase2) were the most highly expressed while expression of DGAT1 was very low, suggesting DGAT2 was the dominant DGAT which catalyzed TAG biosynthesis. To verify these results,10 unigenes were selected and their expression patterns were analyzed by quantitative RTPCR. These data provided comprehensive information for understanding the molecular mechanism of FA and TAG biosynthesis in C. humilis seed.展开更多
【目的】探究欧李Dof(DNA-binding with one zinc finger)锌指蛋白在非生物胁迫条件下的功能,为ChDof基因的研究与利用奠定基础。【方法】基于欧李全基因组和转录组数据对Dof基因家族进行鉴定,并利用实时定量PCR分析Dof基因在非生物胁...【目的】探究欧李Dof(DNA-binding with one zinc finger)锌指蛋白在非生物胁迫条件下的功能,为ChDof基因的研究与利用奠定基础。【方法】基于欧李全基因组和转录组数据对Dof基因家族进行鉴定,并利用实时定量PCR分析Dof基因在非生物胁迫下的表达情况。【结果】欧李共有23个Dof基因家族成员,其编码区CDS序列长度为226~515 bp,相对分子质量为24227.52~55368.21,理论等电点为4.78~9.36,且其在欧李的8条染色体上都有分布。在构建欧李和拟南芥的系统进化树中发现,欧李共分为9个亚组,同一亚族成员中的基因结构和蛋白保守基序均具有较高的相似性,且欧李Dof基因家族成员主要分布在D1亚组中。在对其亚细胞定位时发现,绝大多数ChDof基因主要分布于细胞核中。对欧李Dof家族成员启动子区域顺式调控元件的预测结果表明,其启动子区存在着至少1个激素调节及逆境胁迫反应元件。qRT-PCR分析结果表明,绝大多数的ChDof基因在盐、高温和低温胁迫下的表达量均上调,而ChDof11、ChDof12、ChDof17基因在盐、渗透(PEG)、高温和低温4种胁迫下的表达量均上升,ChDof03基因对高盐胁迫的反应最敏感,ChDof01基因对低温胁迫的响应最敏感,ChDof11基因在渗透、高温和低温胁迫下的表达量均有极显著的提高,而ChDof7和ChDof08基因在4种胁迫诱导下的表达量均降低。【结论】共鉴定出23个Dof家族成员,欧李ChDof11、ChDof12、ChDof17这3个基因在逆境胁迫中均有控制功能,ChDof7和ChDof08这2个基因则在逆境胁迫中均起负调节作用。Dof基因在欧李对逆境胁迫的响应中发挥着重要作用。展开更多
基金funded by the National Natural Science Foundation of China(Grant No.32171737)the Innovation Project of State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University)(Grant No.2021A04)+1 种基金Natural Science Foundation of Heilongjiang Province(Grant No.LH2021C007)the Fundamental Research Funds for the Central Universities(Grant No.2572021DX07)。
文摘Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration.However,the regulatory network of C.humilis in anthocyanin biosynthesis is still unclear.In this study,the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species.Yeast one-hybrid,dual-luciferase assays,and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C.humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS(MYB-binding elements).ChMYB1 interacted with ChbHLH42and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT.In addition,abscisic acid(ABA)treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C.humilis fruit.Interestingly,ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42.Furthermore,ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42.Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C.humilis fruit,deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops.
文摘Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(NSF))were obtained from Chinese dwarf cherry(Cerasus humilis)fruits.All of them were branched low methoxylated pectins with an amorphous or partially nanocrystalline nature and eight neutral monosaccharides(arabinose and galactose were most abundant).WSF,CSF and NSF had a degree of methylation(DM)of 35.82%,14.85%and 7.13%,uronic acid(UA)content of 76.02%,83.71%and 69.01%,and total protein content of 2.4%,2.1%and 8.8%,respectively.Their molecular weights were 340.31,330.16 and 141.31 kg/mol,respectively(analyzed by gel permeation chromatography(GPC)).WSF,CSF and NSF exhibited good rheological,thermal,emulsifying,emulsion-stabilizing,water-adsorbing,oil-binding,cholesterol-binding and antioxidant properties.NSF had the highest emulsifying,emulsion stabilizing,water-/oil-/cholesterol-binding and antioxidant capacities,followed by CSF.NSF had the highest viscosity(406.77 m Pa·s),flowability,and resistance to heat-induced changes/damage,which may be related to its lowest polydispersity index,DM and UA content and highest protein content.The three pectin fractions with desirable characteristics can be used as food additives/ingredients and dietary supplements.
基金supported by the National Key Research and Development Program (2018YFD1000200)the Shanxi Natural Science Foundation (201801D121251)+2 种基金the Key project of Shanxi Key R&D Program (201703D211001-04-04)the Key project of Shanxi Key R&D Program (201703D221028-4)the Outstanding Doctor Award Funding Research Project (SXYBKY2018041)
文摘Cerasus humilis is a woody shrub with increasing economic importance. Due to its high oil content in seed with desired fatty acid(FA) composition, C. humilis has been considered as a potential woody oilseed crop in China. However, FAs accumulation and related molecular mechanisms of FA biosynthesis in C. humilis seed have not been elucidated well. In this study, oil contents and FA compositions of developing C. humilis seed were analyzed. The total oil content in mature seed reached 48.7% while unsaturated FA concentration reached a high level of 96.2%. Three c DNA libraries of C. humilis developing seed were constructed at the beginning and 2 fast oil-accumulation stages, followed by Illumina sequencing with the platform of HiSeq^TM 2000. Differentially expressed unigenes(DEGs) were identified during the respective seed development stages to investigate transcription dynamics. Among DEGs, 82 unigenes were identified as being closely involved in de novo FA and triacylglycerol(TAG) biosynthesis. Surprisingly, among DEGs involved in TAG biosynthesis, expression of unigenes encoding GPATs(glycerol-3-phosphate acyltransferase) were relatively low and unigenes encoding LPAATs(lysophosphatidic acid acyltransferase) were highly expressed, suggesting that LPAAT contributed more in storage lipids metabolism in C. humilis seed. Genes encoding DGAT2(diacylgycerol acyltransferase2) were the most highly expressed while expression of DGAT1 was very low, suggesting DGAT2 was the dominant DGAT which catalyzed TAG biosynthesis. To verify these results,10 unigenes were selected and their expression patterns were analyzed by quantitative RTPCR. These data provided comprehensive information for understanding the molecular mechanism of FA and TAG biosynthesis in C. humilis seed.
文摘【目的】探究欧李Dof(DNA-binding with one zinc finger)锌指蛋白在非生物胁迫条件下的功能,为ChDof基因的研究与利用奠定基础。【方法】基于欧李全基因组和转录组数据对Dof基因家族进行鉴定,并利用实时定量PCR分析Dof基因在非生物胁迫下的表达情况。【结果】欧李共有23个Dof基因家族成员,其编码区CDS序列长度为226~515 bp,相对分子质量为24227.52~55368.21,理论等电点为4.78~9.36,且其在欧李的8条染色体上都有分布。在构建欧李和拟南芥的系统进化树中发现,欧李共分为9个亚组,同一亚族成员中的基因结构和蛋白保守基序均具有较高的相似性,且欧李Dof基因家族成员主要分布在D1亚组中。在对其亚细胞定位时发现,绝大多数ChDof基因主要分布于细胞核中。对欧李Dof家族成员启动子区域顺式调控元件的预测结果表明,其启动子区存在着至少1个激素调节及逆境胁迫反应元件。qRT-PCR分析结果表明,绝大多数的ChDof基因在盐、高温和低温胁迫下的表达量均上调,而ChDof11、ChDof12、ChDof17基因在盐、渗透(PEG)、高温和低温4种胁迫下的表达量均上升,ChDof03基因对高盐胁迫的反应最敏感,ChDof01基因对低温胁迫的响应最敏感,ChDof11基因在渗透、高温和低温胁迫下的表达量均有极显著的提高,而ChDof7和ChDof08基因在4种胁迫诱导下的表达量均降低。【结论】共鉴定出23个Dof家族成员,欧李ChDof11、ChDof12、ChDof17这3个基因在逆境胁迫中均有控制功能,ChDof7和ChDof08这2个基因则在逆境胁迫中均起负调节作用。Dof基因在欧李对逆境胁迫的响应中发挥着重要作用。
