Hypertriglyceridemia is the third leading cause of acute pancreatitis(AP),and its incidence is increasing.Due to its relatively insidious etiology,it is easy to be ignored in the early stages.In China,Chaiqin Chengqi ...Hypertriglyceridemia is the third leading cause of acute pancreatitis(AP),and its incidence is increasing.Due to its relatively insidious etiology,it is easy to be ignored in the early stages.In China,Chaiqin Chengqi Decoction(CQCQD)has long been employed for treating AP.AIM To evaluate the effectiveness of CQCQD in patients diagnosed with mild/moderately severe hypertriglyceridemic AP(HTG-AP).METHODS In this study,the clinical data of 39 patients with HTG-AP admitted from January 2019 to November 2022 were collected.The changes of blood lipids,gastrointestinal symptoms,and abdominal pain before and after treatment were analyzed and compared between the two groups.RESULTS Twenty patients were treated with the conventional HTG-AP regimen,and 19 patients were additionally treated with CQCQD.After receiving treatment,the triglycerides(TG)level of the CQCQD group was lower than that of the CQCQD group(3.14±0.25 mmol/L vs 4.96±0.47 mmol/L,P<0.01).After 3 d of treatment,the patients in the CQCQD group had more bowel movements than the control group(2.51±0.25 times vs 1.00±0.17 times,P=0.01).The gastrointestinal function of most patients returned to normal,and the acute gastrointestinal injury score was significantly lower than that of the control group(0.11±0.07 vs 0.42±0.11,P<0.01).CONCLUSION In patients with HTG-AP,CQCQD can significantly reduce the TG level,shorten the recovery time of defecation,significantly improve the gastrointestinal function.展开更多
Objective:In this paper,we investigated the effect of the traditional Chinese medicine Chaiqin Chengqi Decoction(CQCQD) on serum cytokines in acute pancreatitis(AP) patients.Methods:Peripheral blood samples from 107 A...Objective:In this paper,we investigated the effect of the traditional Chinese medicine Chaiqin Chengqi Decoction(CQCQD) on serum cytokines in acute pancreatitis(AP) patients.Methods:Peripheral blood samples from 107 AP patients were collected within the first 48 h of AP onset and on the 10th day of CQCQD treatment.Control samples were collected from 20 healthy individuals.Serum proinflammatory cytokines tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6),and anti-inflammatory cytokines IL-10 and IL-1β receptor antagonist(IL-1ra) were examined using the Luminex 100 system.Results:Within the first 48 h of AP onset,IL-6 and IL-1ra levels in severe AP(SAP) patients were significantly higher than those in mild AP(MAP) patients,but IL-10 levels in SAP patients were significantly lower than those in MAP patients.Proinflammatory cytokine IL-6 was significantly decreased after CQCQD treatment(P<0.05),especially in SAP patients(n=25 of 36,P<0.05).The hospitalization time of SAP patients was shortened significantly when serum IL-6 decreased after CQCQD treatment(P<0.05).Conclusions:CQCQD decreased proinflammatory cytokine IL-6 levels in AP patients.展开更多
OBJECTIVE: To investigate the effect of Chaiqin Chengqi decoction(CQCQD) on acute pancreatitis(AP) by janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway in vitro and in vivo.METH...OBJECTIVE: To investigate the effect of Chaiqin Chengqi decoction(CQCQD) on acute pancreatitis(AP) by janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway in vitro and in vivo.METHODS: AP was induced by caerulein both in AR42J cells and in mice. AR42J cells were divided into five groups: the control group, the AP group, the CQCQD group, JAK/STAT signaling pathway inhibitor AG490 group, and the CQCQD and AG490 group.After induction, cellular supernatant of five groups were collected for measuring the concentrations of inflammatory cytokine amylase, interleukin 6(IL-6),tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), nuclear factor κB(NF-κB) by enzyme-linked immunosorbent assay and the expression of JAK-2,STAT-3 signaling transduction proteins by Western blot, respectively. Experiments in mice were performed similar to that of in AR42J cells.RESULTS: Treatment of AR42J cells with CQCQD reduced the pancreatic injury and negatively regulated the activities of amylase, as well as inhibited expression of several inflammatory cytokines such as IL-6, TNF-α, IL-1β, NF-κB. Administration of CQCQD significantly inhibited JAK-2 activation and down-regulated phosphorylation of downstream substrate STAT-3 the same as AG490, resulting in inhibition of inflammatory mediators and amelioration of pancreatitis.CONCLUSION: The results suggested that CQCQD exerted anti-inflammatory effects on AP via reducing expression and phosphorylation of JAK and STAT.展开更多
OBJECTIVE: To explore the effect and the mechanism of Chaiqinchengqi decoction(CQCQD) on the apoptosis-necrosis switch of pancreatic acinar cells in acute necrotizing pancreatitis(ANP) in rats.METHODS: Sixty Sprague-D...OBJECTIVE: To explore the effect and the mechanism of Chaiqinchengqi decoction(CQCQD) on the apoptosis-necrosis switch of pancreatic acinar cells in acute necrotizing pancreatitis(ANP) in rats.METHODS: Sixty Sprague-Dawley rats were randomized into the control group, the ANP group and the CQCQD group. The acute pancreatitis(AP)model was induced by intraperitoneal injections of4 g/kg 8% L-Arginine(PH 7.0) twice with a 1 h interval. Rats in the CQCQD group were intragastrically administered CQCQD(20 mL/kg every 2 h, 3 times,then 20 mL/kg every 6 h, 3 times). Rats were killed at the 6 and 24 h after the induction of AP.The pancreatic tissues were collected for pathology and to isolate pancreatic acinar cells and mitochondria.RESULTS: CQCQD significantly ameliorated the severity of ANP by reducing the pancreatic histopathology score, indicated by lactate dehydrogenase levels at the 6 and 24 h. The CQCQD group promoted the apoptosis of pancreatic acinar cells by raising the apoptosis index compared with the ANP group and the control group. Mitochondrial cytochrome c at the 6 and 24 h in the ANP group were lower than that in the control group or the CQCQD group(0.67±0.13 vs 1.54±0.03 vs 0.81±0.09; 0.71±0.08 vs 1.55±0.09 vs 0.89±0.16, P<0.01). The cytochrome c levels in the cytoplasm at the 6 and 2 h in the CQCQD group were higher than in the control group(1.36±0.15 vs 0.67±0.04, 1.46±0.08 vs 0.59±0.09, P<0.01), or the ANP group(0.96±0.13, P>0.05;0.97±0.09, P<0.05). CQCQD increased caspase-3 activity over the ANP group at the 6 h.CONCLUSION: CQCQD can induce apoptosis and relieve the necrosis of pancreatic acinar cells via promoting the release of mitochondrial cytochrome c and increasing pancreatic caspase-3 activity in ANP rats.展开更多
Objective:To investigate the effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD)on regulating serum matrix metalloproteinase 9(MMP-9)in patients with severe acute pancreatitis(SAP).Methods:Thirty-five SAP patients h...Objective:To investigate the effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD)on regulating serum matrix metalloproteinase 9(MMP-9)in patients with severe acute pancreatitis(SAP).Methods:Thirty-five SAP patients hospitalized in West China Hospital from September 1,2008 to February 28,2009 were randomly assigned to two groups using a computer-derived random number sequence in a ratio of 1:1,treatment group(18patients)and the placebo control group(17 patients).The patients in the treatment group were administered with CQCQD by gastric perfusion(50 mL/2 h)and retention enema(200 mL/6 h)for 7 days.The two groups had similar baseline information.The clinical indicators,including the initial Balthazar's computed tomography(CT)score,acute physiology and chronic health evaluationⅡ(APACHEⅡ)scores on 1st,3rd,5th and 7th day,incidences and durations of complications and the serum C-reactive protein(CRP),levels of MMP-9 on the 1st,3rd,5th and 7th day,were recorded and compared between the two groups.Results:The serum MMP-9,CRP and the APACHEⅡscores on the 3rd,5th and 7th day in the treatment group were lower than those in the control group(P<0.05).The serum MMP-9 was positively correlated with the APACHEⅡscore on the 1st day(r=0.430,P=0.01).The durations of acute respiratory distress syndrome(5.4±2.4 vs.2.9±1.3),acute hepatitis(4.6±0.8 vs.1.9±0.6)and acute heart failure(3.9±1.6 vs.1.3±0.6,P<0.05)in the control group were longer than those in the treatment group.Conclusion:CQCQD could decrease the serum MMP-9 to relieve the severity of clinical symptoms and prevent the development of multiple organ dysfunction syndrome in patients with SAP.展开更多
Objective:To investigate effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD) on changes of neuronal acetylcholine receptor alpha 7(nAChRα7) of peritoneal macrophages in acute pancreatitis(AP).Methods:Eighteen Kunmi...Objective:To investigate effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD) on changes of neuronal acetylcholine receptor alpha 7(nAChRα7) of peritoneal macrophages in acute pancreatitis(AP).Methods:Eighteen Kunming mice were equally randomized into the control group,AP group and CQCQD treatment group.AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart,while control mice received saline injections.At 72 h after the first injection of L-arginine,mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times,whilst mice in the other two groups received the same amount of saline feeding.Mice were sacrificed by cervical dislocation 2 h after the last feeding of either CQCQD or saline.Peritoneal macrophages were collected for determination of nAChRα7 mRNA and protein expression.Serum was collected for detection of interleukin-6(IL-6),IL-10 and acetylcholine(ACh)levels,and pancreas was for histopathology analysis.Results:The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score(4.5 ± 0.5 vs.6.2 ± 1.7,P<0.05)and the serum IL-6 levels(1228.31419.2 pg/mL vs.1589.6 ±337.3 pg/mL,P<0.05).The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group(P>0.05),but were significantly up-regulated after the CQCQD treatment(P<0.05).The serum ACh levels in the AP group were significantly lower than those in the control group(3.1 ± 0.6 μg/mL vs 4.8 ± 0.7 μg/mL P<0.05),but were significantly increased after the CQCQD treatment(5.6±1.5 μg/mL vs 3.1 ±0.6 μg/mL,P<0.05).Conclusion:CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChR α 7 of peritoneal macrophages.展开更多
Objective:To investigate the effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD)on cholecystokinin receptor 1(CCKRI)-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis(AN...Objective:To investigate the effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD)on cholecystokinin receptor 1(CCKRI)-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis(ANP).Methods:Twenty-seven Sprague-Dawley rats were randomized into three groups:the control group,the ANP group,and the CQCQD group(9 in each group).ANP rats were induced by two intraperitoneal injections of 8%L-arginine(pH=7.0,4.4 g/kg) over a 2-h period.Rats were treated with 1.5 mL/100 g body weight of CQCQD(CQCQD group) or physiological saline(control and ANP groups) at 2 h interval.And 6 h after induction,pancreatic tissues were collected for histopathological examination.Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression,phospholipase C(PLC) and inositol-1,4,5-triphosphate(IP3),and determination of fluorescence intensity(Fl)as a measure of intracellular calcium ion concentration[Ca^(2+)]_i.Results:The pancreatic histopathological score(6.2 + 1.1) and the levels of PLC(1,187.2 ±228.2 μg/mL) and IP3(872.2 ±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control(2.8 ±0.4,682.5 ±121.8 μg/mL,518.4 ±115.8 μg/mL)and the CQCQD(3.8 ±0.8,905.3 ±78.5 μg/mL,611.0 ±42.5 μg/mL) groups(P<0.05).[Ca^(2+)]_i Fl for the ANP group(34.8 ±27.0) was higher than that in the control(5.1 ±2.2) and CQCQD(12.6 ±2.5) groups(P<0.05).The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated(expression ratio=1.761;P=0.024) compared with the control group.The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated(expression ratio=0.311;P=0.035) compared with the ANP group.The ratio of gray values of the CCKR1 and B-actin in the ANP group(1.43 ±0.17) was higher than those in the control(0.70 ±0.15) and CQCQD(0.79 ±0.11) groups(P<0.05).Conclusions:Pancreatic acinar cell calcium overload of ANP induced by L-arginine was related to the up-regulated expressions of pancreatic acinar cell CCKR1 mRNA and protein.CQCQD can down-regulate expressions of pancreatic acinar cell CCKR1 mRNA and protein to reduce the PLC and IP3 of pancreatic acinar cells,relieving the calcium overload and reducing the pathological changes in rats with ANP.展开更多
目的系统评价柴芩承气汤联合西药治疗急性胰腺炎(Acute Pancreatitis,AP)的有效性与安全性。方法检索中国知网(CNKI)、维普(VIP)、万方(WanFang)、中国生物医学文献数据库(CBM)、PubMed、EMbase、Web of Science、Cochrane Library数据...目的系统评价柴芩承气汤联合西药治疗急性胰腺炎(Acute Pancreatitis,AP)的有效性与安全性。方法检索中国知网(CNKI)、维普(VIP)、万方(WanFang)、中国生物医学文献数据库(CBM)、PubMed、EMbase、Web of Science、Cochrane Library数据库,搜集柴芩承气汤联合西药治疗AP的随机对照试验,检索时限均为建库至2021年2月。由2名研究者独立筛选文献、提取资料并进行偏倚风险评价后,采用Revman 5.4软件进行Meta分析。结果共纳入17篇RCT,包括1572例患者。Meta分析结果显示:与常规西药治疗相比,柴芩承气汤联合治疗AP可提高总有效率,进一步按疾病严重程度进行亚组分析,结果显示柴芩承气汤联合治疗可提高轻症急性胰腺炎、重症急性胰腺炎的有效率;此外,还可降低并发症发生率;缩短腹痛缓解时间、首次排便时间;缩短平均住院时间。结论当前证据表明,柴芩承气汤联合西药治疗AP,能提高临床总有效率、减少并发症,缩短腹痛缓解时间、首次排便时间,缩短平均住院时间,且安全性较好。提示柴芩承气汤联合西药在治疗AP过程中比单纯西药治疗更具优势。受纳入研究所限,上述结论尚需更多高质量研究进一步验证。展开更多
目的系统评价柴芩承气汤联合常规西医治疗急性胰腺炎的有效性和安全性。方法计算机检索发表在PubMed、Embase、Cochrane Library、Web of Science、中国知网(CNKI)、万方(WanFang)、中国生物医学文献数据库(CBM)、维普(VIP)数据库上的...目的系统评价柴芩承气汤联合常规西医治疗急性胰腺炎的有效性和安全性。方法计算机检索发表在PubMed、Embase、Cochrane Library、Web of Science、中国知网(CNKI)、万方(WanFang)、中国生物医学文献数据库(CBM)、维普(VIP)数据库上的相关文献,检索时限为建库至2020年12月。根据纳入排除标准进行文献筛选和数据提取。采用Cochrane偏倚风险评估表评价文献质量,运用RevMan 5.4软件进行Meta分析。结果共纳入11项研究,共994例患者。Meta分析结果显示,试验组能够提高治疗总有效率,降低血清淀粉酶水平,减少住院时间、缩短腹痛缓解时间,且各方面均优于对照组,在不良反应发生率方面差异无统计学意义。结论柴芩承气汤联合西医常规治疗急性胰腺炎优于单纯西药疗法,但不良反应需进一步评价。展开更多
基金The Hangzhou Science and Technology Bureau,No.B20230285.
文摘Hypertriglyceridemia is the third leading cause of acute pancreatitis(AP),and its incidence is increasing.Due to its relatively insidious etiology,it is easy to be ignored in the early stages.In China,Chaiqin Chengqi Decoction(CQCQD)has long been employed for treating AP.AIM To evaluate the effectiveness of CQCQD in patients diagnosed with mild/moderately severe hypertriglyceridemic AP(HTG-AP).METHODS In this study,the clinical data of 39 patients with HTG-AP admitted from January 2019 to November 2022 were collected.The changes of blood lipids,gastrointestinal symptoms,and abdominal pain before and after treatment were analyzed and compared between the two groups.RESULTS Twenty patients were treated with the conventional HTG-AP regimen,and 19 patients were additionally treated with CQCQD.After receiving treatment,the triglycerides(TG)level of the CQCQD group was lower than that of the CQCQD group(3.14±0.25 mmol/L vs 4.96±0.47 mmol/L,P<0.01).After 3 d of treatment,the patients in the CQCQD group had more bowel movements than the control group(2.51±0.25 times vs 1.00±0.17 times,P=0.01).The gastrointestinal function of most patients returned to normal,and the acute gastrointestinal injury score was significantly lower than that of the control group(0.11±0.07 vs 0.42±0.11,P<0.01).CONCLUSION In patients with HTG-AP,CQCQD can significantly reduce the TG level,shorten the recovery time of defecation,significantly improve the gastrointestinal function.
基金Project supported by the National Natural Science Foundation of China (Nos. 30830100 and 30972924)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (No. 20101174-4-2)
文摘Objective:In this paper,we investigated the effect of the traditional Chinese medicine Chaiqin Chengqi Decoction(CQCQD) on serum cytokines in acute pancreatitis(AP) patients.Methods:Peripheral blood samples from 107 AP patients were collected within the first 48 h of AP onset and on the 10th day of CQCQD treatment.Control samples were collected from 20 healthy individuals.Serum proinflammatory cytokines tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6),and anti-inflammatory cytokines IL-10 and IL-1β receptor antagonist(IL-1ra) were examined using the Luminex 100 system.Results:Within the first 48 h of AP onset,IL-6 and IL-1ra levels in severe AP(SAP) patients were significantly higher than those in mild AP(MAP) patients,but IL-10 levels in SAP patients were significantly lower than those in MAP patients.Proinflammatory cytokine IL-6 was significantly decreased after CQCQD treatment(P<0.05),especially in SAP patients(n=25 of 36,P<0.05).The hospitalization time of SAP patients was shortened significantly when serum IL-6 decreased after CQCQD treatment(P<0.05).Conclusions:CQCQD decreased proinflammatory cytokine IL-6 levels in AP patients.
基金Supported by the National S&T Major Project of China,Construction of Technical Platform for New Preparation and New Release Drug System in Compliance with GLP Requirements(No.2012ZX09304004001)
文摘OBJECTIVE: To investigate the effect of Chaiqin Chengqi decoction(CQCQD) on acute pancreatitis(AP) by janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway in vitro and in vivo.METHODS: AP was induced by caerulein both in AR42J cells and in mice. AR42J cells were divided into five groups: the control group, the AP group, the CQCQD group, JAK/STAT signaling pathway inhibitor AG490 group, and the CQCQD and AG490 group.After induction, cellular supernatant of five groups were collected for measuring the concentrations of inflammatory cytokine amylase, interleukin 6(IL-6),tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), nuclear factor κB(NF-κB) by enzyme-linked immunosorbent assay and the expression of JAK-2,STAT-3 signaling transduction proteins by Western blot, respectively. Experiments in mice were performed similar to that of in AR42J cells.RESULTS: Treatment of AR42J cells with CQCQD reduced the pancreatic injury and negatively regulated the activities of amylase, as well as inhibited expression of several inflammatory cytokines such as IL-6, TNF-α, IL-1β, NF-κB. Administration of CQCQD significantly inhibited JAK-2 activation and down-regulated phosphorylation of downstream substrate STAT-3 the same as AG490, resulting in inhibition of inflammatory mediators and amelioration of pancreatitis.CONCLUSION: The results suggested that CQCQD exerted anti-inflammatory effects on AP via reducing expression and phosphorylation of JAK and STAT.
基金Supported by National Natural Science Foundation of China (No.81072910)Science and Technology Support Program of Sichuan (No.2011SZ0291)
文摘OBJECTIVE: To explore the effect and the mechanism of Chaiqinchengqi decoction(CQCQD) on the apoptosis-necrosis switch of pancreatic acinar cells in acute necrotizing pancreatitis(ANP) in rats.METHODS: Sixty Sprague-Dawley rats were randomized into the control group, the ANP group and the CQCQD group. The acute pancreatitis(AP)model was induced by intraperitoneal injections of4 g/kg 8% L-Arginine(PH 7.0) twice with a 1 h interval. Rats in the CQCQD group were intragastrically administered CQCQD(20 mL/kg every 2 h, 3 times,then 20 mL/kg every 6 h, 3 times). Rats were killed at the 6 and 24 h after the induction of AP.The pancreatic tissues were collected for pathology and to isolate pancreatic acinar cells and mitochondria.RESULTS: CQCQD significantly ameliorated the severity of ANP by reducing the pancreatic histopathology score, indicated by lactate dehydrogenase levels at the 6 and 24 h. The CQCQD group promoted the apoptosis of pancreatic acinar cells by raising the apoptosis index compared with the ANP group and the control group. Mitochondrial cytochrome c at the 6 and 24 h in the ANP group were lower than that in the control group or the CQCQD group(0.67±0.13 vs 1.54±0.03 vs 0.81±0.09; 0.71±0.08 vs 1.55±0.09 vs 0.89±0.16, P<0.01). The cytochrome c levels in the cytoplasm at the 6 and 2 h in the CQCQD group were higher than in the control group(1.36±0.15 vs 0.67±0.04, 1.46±0.08 vs 0.59±0.09, P<0.01), or the ANP group(0.96±0.13, P>0.05;0.97±0.09, P<0.05). CQCQD increased caspase-3 activity over the ANP group at the 6 h.CONCLUSION: CQCQD can induce apoptosis and relieve the necrosis of pancreatic acinar cells via promoting the release of mitochondrial cytochrome c and increasing pancreatic caspase-3 activity in ANP rats.
基金Supported by Science and Technology Support Program of Sichuan(No.2011SZ0291,2010SZ0068)
文摘Objective:To investigate the effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD)on regulating serum matrix metalloproteinase 9(MMP-9)in patients with severe acute pancreatitis(SAP).Methods:Thirty-five SAP patients hospitalized in West China Hospital from September 1,2008 to February 28,2009 were randomly assigned to two groups using a computer-derived random number sequence in a ratio of 1:1,treatment group(18patients)and the placebo control group(17 patients).The patients in the treatment group were administered with CQCQD by gastric perfusion(50 mL/2 h)and retention enema(200 mL/6 h)for 7 days.The two groups had similar baseline information.The clinical indicators,including the initial Balthazar's computed tomography(CT)score,acute physiology and chronic health evaluationⅡ(APACHEⅡ)scores on 1st,3rd,5th and 7th day,incidences and durations of complications and the serum C-reactive protein(CRP),levels of MMP-9 on the 1st,3rd,5th and 7th day,were recorded and compared between the two groups.Results:The serum MMP-9,CRP and the APACHEⅡscores on the 3rd,5th and 7th day in the treatment group were lower than those in the control group(P<0.05).The serum MMP-9 was positively correlated with the APACHEⅡscore on the 1st day(r=0.430,P=0.01).The durations of acute respiratory distress syndrome(5.4±2.4 vs.2.9±1.3),acute hepatitis(4.6±0.8 vs.1.9±0.6)and acute heart failure(3.9±1.6 vs.1.3±0.6,P<0.05)in the control group were longer than those in the treatment group.Conclusion:CQCQD could decrease the serum MMP-9 to relieve the severity of clinical symptoms and prevent the development of multiple organ dysfunction syndrome in patients with SAP.
基金Supported by National Natural Science Foundation of China(No.81072910)Science and Technology Support Program of Sichuan(No.2011SZ0291,2010SZ0068)
文摘Objective:To investigate effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD) on changes of neuronal acetylcholine receptor alpha 7(nAChRα7) of peritoneal macrophages in acute pancreatitis(AP).Methods:Eighteen Kunming mice were equally randomized into the control group,AP group and CQCQD treatment group.AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart,while control mice received saline injections.At 72 h after the first injection of L-arginine,mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times,whilst mice in the other two groups received the same amount of saline feeding.Mice were sacrificed by cervical dislocation 2 h after the last feeding of either CQCQD or saline.Peritoneal macrophages were collected for determination of nAChRα7 mRNA and protein expression.Serum was collected for detection of interleukin-6(IL-6),IL-10 and acetylcholine(ACh)levels,and pancreas was for histopathology analysis.Results:The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score(4.5 ± 0.5 vs.6.2 ± 1.7,P<0.05)and the serum IL-6 levels(1228.31419.2 pg/mL vs.1589.6 ±337.3 pg/mL,P<0.05).The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group(P>0.05),but were significantly up-regulated after the CQCQD treatment(P<0.05).The serum ACh levels in the AP group were significantly lower than those in the control group(3.1 ± 0.6 μg/mL vs 4.8 ± 0.7 μg/mL P<0.05),but were significantly increased after the CQCQD treatment(5.6±1.5 μg/mL vs 3.1 ±0.6 μg/mL,P<0.05).Conclusion:CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChR α 7 of peritoneal macrophages.
基金Supported by the National Natural Science Foundation of China(No.81072910)the Science and Technology Supported Program of Sichuan Province,China(No.2010SZ0068 and 2011SZ0291)
文摘Objective:To investigate the effect of Chaiqin Chengqi Decoction(柴芩承气汤,CQCQD)on cholecystokinin receptor 1(CCKRI)-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis(ANP).Methods:Twenty-seven Sprague-Dawley rats were randomized into three groups:the control group,the ANP group,and the CQCQD group(9 in each group).ANP rats were induced by two intraperitoneal injections of 8%L-arginine(pH=7.0,4.4 g/kg) over a 2-h period.Rats were treated with 1.5 mL/100 g body weight of CQCQD(CQCQD group) or physiological saline(control and ANP groups) at 2 h interval.And 6 h after induction,pancreatic tissues were collected for histopathological examination.Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression,phospholipase C(PLC) and inositol-1,4,5-triphosphate(IP3),and determination of fluorescence intensity(Fl)as a measure of intracellular calcium ion concentration[Ca^(2+)]_i.Results:The pancreatic histopathological score(6.2 + 1.1) and the levels of PLC(1,187.2 ±228.2 μg/mL) and IP3(872.2 ±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control(2.8 ±0.4,682.5 ±121.8 μg/mL,518.4 ±115.8 μg/mL)and the CQCQD(3.8 ±0.8,905.3 ±78.5 μg/mL,611.0 ±42.5 μg/mL) groups(P<0.05).[Ca^(2+)]_i Fl for the ANP group(34.8 ±27.0) was higher than that in the control(5.1 ±2.2) and CQCQD(12.6 ±2.5) groups(P<0.05).The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated(expression ratio=1.761;P=0.024) compared with the control group.The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated(expression ratio=0.311;P=0.035) compared with the ANP group.The ratio of gray values of the CCKR1 and B-actin in the ANP group(1.43 ±0.17) was higher than those in the control(0.70 ±0.15) and CQCQD(0.79 ±0.11) groups(P<0.05).Conclusions:Pancreatic acinar cell calcium overload of ANP induced by L-arginine was related to the up-regulated expressions of pancreatic acinar cell CCKR1 mRNA and protein.CQCQD can down-regulate expressions of pancreatic acinar cell CCKR1 mRNA and protein to reduce the PLC and IP3 of pancreatic acinar cells,relieving the calcium overload and reducing the pathological changes in rats with ANP.
文摘目的系统评价柴芩承气汤联合常规西医治疗急性胰腺炎的有效性和安全性。方法计算机检索发表在PubMed、Embase、Cochrane Library、Web of Science、中国知网(CNKI)、万方(WanFang)、中国生物医学文献数据库(CBM)、维普(VIP)数据库上的相关文献,检索时限为建库至2020年12月。根据纳入排除标准进行文献筛选和数据提取。采用Cochrane偏倚风险评估表评价文献质量,运用RevMan 5.4软件进行Meta分析。结果共纳入11项研究,共994例患者。Meta分析结果显示,试验组能够提高治疗总有效率,降低血清淀粉酶水平,减少住院时间、缩短腹痛缓解时间,且各方面均优于对照组,在不良反应发生率方面差异无统计学意义。结论柴芩承气汤联合西医常规治疗急性胰腺炎优于单纯西药疗法,但不良反应需进一步评价。
