Auxin plays critical roles in root formation and development. The components involved in this process,however, are not well understood. Here, we newly identified a peptide encoding gene, auxin‐responsive endogenous p...Auxin plays critical roles in root formation and development. The components involved in this process,however, are not well understood. Here, we newly identified a peptide encoding gene, auxin‐responsive endogenous polypeptide 1(AREP1), which is induced by auxin, and mediates root development in Arabidopsis. Expression of AREP1 was specific to the cotyledon and to root and shoot meristem tissues. Amounts of AREP1 transcripts and AREP1‐green fluorescent protein fusion proteins were elevated in response to indoleacetic acid treatment. Suppression of AREP1 through RNAi silencing resulted in reduction of primary root length, increase of lateral root number, and expansion of adventitious roots, compared to the observations in wild‐type plants in the presence of auxin. By contrast, transgenic plants overexpressing AREP1 showed enhanced growth of the primary root under auxin treatment.Additionally, root morphology, including lateral root number and adventitious roots, differed greatly between transgenic and wild‐type plants. Further analysis indicated that the expression of auxin‐responsive genes, such as IAA3, IAA7, IAA17, GH3.2, GH3.3,and SAUR‐AC1, was significantly higher in AREP1 RNAi plants, and was slightly lower in AREP1 overexpressing plants than in wild‐type plants. These results suggest that the novel endogenous peptide AREP1 plays an important role in the process of auxin‐mediated root development.展开更多
文摘志丹杏(Armeniaca zhidanensis C.Z.Qiao et Y.P.Zhu)是作者在苦杏仁原植物调查鉴定中发现的杏属植物一新种,并已在商品苦杏仁中鉴定出志丹吉。本文分析了志丹杏和药典收载的4种苦杏仁原植物杏(A.mugaris Lam.)、野杏(A.mugaris Lam.var ansu(Maxim.)Yh et Lu)、西伯利亚杏(A.sibirica(L.)Lam.)和东北杏(A·mandshurica(Maxim,)Skv.)种子的苦杏仁甙含量和脂肪油脂肪酸组成,以评价志丹杏种子是否可以作为苦杏仁入药,结果报道如下:
基金funded by the National Natural Science Foundation of China(30971557,30971816,and31300996)the Guangdong Natural Science Foundation(S2011010001433)
文摘Auxin plays critical roles in root formation and development. The components involved in this process,however, are not well understood. Here, we newly identified a peptide encoding gene, auxin‐responsive endogenous polypeptide 1(AREP1), which is induced by auxin, and mediates root development in Arabidopsis. Expression of AREP1 was specific to the cotyledon and to root and shoot meristem tissues. Amounts of AREP1 transcripts and AREP1‐green fluorescent protein fusion proteins were elevated in response to indoleacetic acid treatment. Suppression of AREP1 through RNAi silencing resulted in reduction of primary root length, increase of lateral root number, and expansion of adventitious roots, compared to the observations in wild‐type plants in the presence of auxin. By contrast, transgenic plants overexpressing AREP1 showed enhanced growth of the primary root under auxin treatment.Additionally, root morphology, including lateral root number and adventitious roots, differed greatly between transgenic and wild‐type plants. Further analysis indicated that the expression of auxin‐responsive genes, such as IAA3, IAA7, IAA17, GH3.2, GH3.3,and SAUR‐AC1, was significantly higher in AREP1 RNAi plants, and was slightly lower in AREP1 overexpressing plants than in wild‐type plants. These results suggest that the novel endogenous peptide AREP1 plays an important role in the process of auxin‐mediated root development.
