The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.The...The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.展开更多
Identifying the geographic origins of Chinese mitten crabs is important for food safety and fair market competition.In this study,we used strontium(Sr) isotopes as a tool to trace the geographic origins of Chinese mit...Identifying the geographic origins of Chinese mitten crabs is important for food safety and fair market competition.In this study,we used strontium(Sr) isotopes as a tool to trace the geographic origins of Chinese mitten crabs.Chinese mitten crabs,water,and different types of crab feed were collected from four different lakes for Sr isotope analyses.The results showed that the Sr isotope compositions of the different parts from one single crab were consistent within error,indicating that any piece of a crab could be used to represent the Sr isotope characteristics of the whole crab.The Sr isotope compositions of Chinese mitten crabs from the same lake were consistent within the analytical error,and the values were similar to the Sr isotope composition of the water from the same lake.However,the Sr isotope compositions of water and crabs from different lakes are significantly different.Therefore,the Sr isotope compositions of Chinese mitten crabs are mainly controlled by lake water composition,while the impact of feed is limited.This study provides an effective method for tracing the geographic origins of Chinese mitten crabs.展开更多
This study aimed to elucidate the effect of a pellet feed containing fermented proteins on pond-raised Chinese mitten crabs,Eriocheir sinensis in a 7-month crab feeding experiment in Gucheng Lake,Jiangsu province,Chin...This study aimed to elucidate the effect of a pellet feed containing fermented proteins on pond-raised Chinese mitten crabs,Eriocheir sinensis in a 7-month crab feeding experiment in Gucheng Lake,Jiangsu province,China.The results showed that fermented protein could significantly increase mean weight gain,crab size and total yield compared with control group.Moreover,the mean weight of male crabs and the number of large-sized male crabs improved over those of the control group.However,feed efficiency ratio did not differ significantly between the two groups.The amino acid content in the muscles of the female crabs of both the groups was higher than that in the muscles of the male crabs.The present study suggests that fermented compound protein may be one kind of potential protein sources for E.sinensis.展开更多
基金supported by the earmarked fund for the Anhui Provincial Modern Agri-industry Technology Research System(AHCYJSTX-08)the China Agriculture Research System of MOF and MARA(CARS-48)。
文摘The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.
基金financially supported by the National Key Research and Development Program of Chinathe project of Source Identification and Contamination Characteristics of Heavy Metals in Agricultural Land and Products (2016YFD0800300)by Natural Science Foundation of China (41721002)
文摘Identifying the geographic origins of Chinese mitten crabs is important for food safety and fair market competition.In this study,we used strontium(Sr) isotopes as a tool to trace the geographic origins of Chinese mitten crabs.Chinese mitten crabs,water,and different types of crab feed were collected from four different lakes for Sr isotope analyses.The results showed that the Sr isotope compositions of the different parts from one single crab were consistent within error,indicating that any piece of a crab could be used to represent the Sr isotope characteristics of the whole crab.The Sr isotope compositions of Chinese mitten crabs from the same lake were consistent within the analytical error,and the values were similar to the Sr isotope composition of the water from the same lake.However,the Sr isotope compositions of water and crabs from different lakes are significantly different.Therefore,the Sr isotope compositions of Chinese mitten crabs are mainly controlled by lake water composition,while the impact of feed is limited.This study provides an effective method for tracing the geographic origins of Chinese mitten crabs.
基金This study was supported by Science and Technology Planning Project of Tianjin City,China(2018YFD0500603).
文摘This study aimed to elucidate the effect of a pellet feed containing fermented proteins on pond-raised Chinese mitten crabs,Eriocheir sinensis in a 7-month crab feeding experiment in Gucheng Lake,Jiangsu province,China.The results showed that fermented protein could significantly increase mean weight gain,crab size and total yield compared with control group.Moreover,the mean weight of male crabs and the number of large-sized male crabs improved over those of the control group.However,feed efficiency ratio did not differ significantly between the two groups.The amino acid content in the muscles of the female crabs of both the groups was higher than that in the muscles of the male crabs.The present study suggests that fermented compound protein may be one kind of potential protein sources for E.sinensis.
