Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage cause...Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apudnic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.展开更多
目的探讨血补体C1q/肿瘤坏死因子相关蛋白1(CTRP1),血同型半胱氨酸(Homocysteine,Hcy)预测脑小血管疾病(cerebral small vessel disease,CSVD)患者血管性轻度认知障碍(vascular mild cognitive impairment,VaMCI)的临床价值。方法选取2...目的探讨血补体C1q/肿瘤坏死因子相关蛋白1(CTRP1),血同型半胱氨酸(Homocysteine,Hcy)预测脑小血管疾病(cerebral small vessel disease,CSVD)患者血管性轻度认知障碍(vascular mild cognitive impairment,VaMCI)的临床价值。方法选取2015年12月~2019年12月沧州市人民医院收治的98例CSVD患者,采用蒙特利尔评估表(Montreal cognitive assessment,MoCA)对其进行认知能力检查,分为VaMCI组(n=56)和认知正常组(n=42)。分别采取Logistic回归分析以及Pearson相关性分析CTRP1、血同型半胱氨酸与VaMCI之间的相关性。结果VaMCI组Hcy,CTRP1以及UA水平均高于认知正常组(Hcy:22.02±3.74μmol/L vs 18.43±3.52μmol/L,CTRP1:162.54±14.85ng/ml vs 135.26±13.41ng/ml,UA:360.27±23.28μmol/L vs 320.55±20.23μmol/L),差异均有统计学意义(t=4.822,9.377,8.833,均P<0.001)。Logistic回归分析结果显示Hcy,CTRP1,UA均是CSVD患者发生VaMCI的危险因素(Hcy:OR=2.782,95%CI:1.515~5.107,P=0.001;CTRP1:OR=3.401,95%CI:1.729~6.687,P=0.000;UA:OR=2.335,95%CI:1.325~4.114,P=0.003);且VaMCI组患者Hcy,CTRP1均与MoCA总分呈负相关(r=-0.415,-0.467,均P<0.05);另VaMCI组患者Hcy,CTRP1水平与记忆、语言以及视空间与执行能力等亚项均呈负相关(r=-0.402,-0.436;-0.365,-0.379;-0.512,-0.536,均P<0.05)。结论CSVD患者伴VaMCI的血清CTRP1,Hcy表达水平明显升高,且两者均属于CSVD患者发生VaMCI的独立危险因素,均与患者的MoCA总分、记忆、语言以及视空间与执行能力呈显著负相关性,早期检测CSVD血清CTRP1和Hcy水平对于预防认知功能障碍发生具有一定价值。展开更多
AIM: To evaluate the association of complement factor H(CFH) and microtubule-associated protein 1 light chain 3 beta(MAP1LC3B) gene polymorphisms with the risk of age-related macular degeneration(AMD) in a high-altitu...AIM: To evaluate the association of complement factor H(CFH) and microtubule-associated protein 1 light chain 3 beta(MAP1LC3B) gene polymorphisms with the risk of age-related macular degeneration(AMD) in a high-altitude population. METHODS: The study group consisted of 172 participants with symptoms of AMD who were examined and diagnosed between January 2019 and June 2020. The control group was composed of 120 healthy individuals. Each participant was required to provide two milliliters of peripheral blood for DNA extraction. Two single nucleotide polymorphisms(SNPs) of CFH(rs1061170 and rs800292) and two SNPs of MAP1LC3B(rs8044820 and rs9903) were genotyped. The genotypes and allele frequencies of the SNPs in the study and control groups were further compared using Chi-square and Fisher’s exact tests. RESULTS: In a high-altitude population, the nominally significant differences of rs800292 and rs9903’s genotype AG frequencies were observed in the AMD group(P=0.034 and 0.004, respectively). The frequencies of allele G of rs800292 and allele A of rs9903 were also significantly dif ferent in the AMD group compared to the control [(P=0.034, OR=0.70, 95%CI: 0.50-0.98) and(P=0.004, OR=1.60, 95%CI: 1.15-2.22), respectively]. No significant differences in the genotype distributions(P=0.16 and 0.40, respectively) and allele frequencies(P>0.05) of rs1061170 and rs8044820 were observed in the AMD group.CONCLUSION: Genotype AG of rs800292 may be a protective factor for AMD. Conversely, rs9903 seems to be a risk factor for AMD. Therefore, allele G of rs800292 may be a protective factor, and allele A of rs9903, a risk factor for AMD in Qinghai high-altitude population.展开更多
基金supported by the National Natural Science Foundation of China, No. 30571790
文摘Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apudnic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.
文摘目的探讨血补体C1q/肿瘤坏死因子相关蛋白1(CTRP1),血同型半胱氨酸(Homocysteine,Hcy)预测脑小血管疾病(cerebral small vessel disease,CSVD)患者血管性轻度认知障碍(vascular mild cognitive impairment,VaMCI)的临床价值。方法选取2015年12月~2019年12月沧州市人民医院收治的98例CSVD患者,采用蒙特利尔评估表(Montreal cognitive assessment,MoCA)对其进行认知能力检查,分为VaMCI组(n=56)和认知正常组(n=42)。分别采取Logistic回归分析以及Pearson相关性分析CTRP1、血同型半胱氨酸与VaMCI之间的相关性。结果VaMCI组Hcy,CTRP1以及UA水平均高于认知正常组(Hcy:22.02±3.74μmol/L vs 18.43±3.52μmol/L,CTRP1:162.54±14.85ng/ml vs 135.26±13.41ng/ml,UA:360.27±23.28μmol/L vs 320.55±20.23μmol/L),差异均有统计学意义(t=4.822,9.377,8.833,均P<0.001)。Logistic回归分析结果显示Hcy,CTRP1,UA均是CSVD患者发生VaMCI的危险因素(Hcy:OR=2.782,95%CI:1.515~5.107,P=0.001;CTRP1:OR=3.401,95%CI:1.729~6.687,P=0.000;UA:OR=2.335,95%CI:1.325~4.114,P=0.003);且VaMCI组患者Hcy,CTRP1均与MoCA总分呈负相关(r=-0.415,-0.467,均P<0.05);另VaMCI组患者Hcy,CTRP1水平与记忆、语言以及视空间与执行能力等亚项均呈负相关(r=-0.402,-0.436;-0.365,-0.379;-0.512,-0.536,均P<0.05)。结论CSVD患者伴VaMCI的血清CTRP1,Hcy表达水平明显升高,且两者均属于CSVD患者发生VaMCI的独立危险因素,均与患者的MoCA总分、记忆、语言以及视空间与执行能力呈显著负相关性,早期检测CSVD血清CTRP1和Hcy水平对于预防认知功能障碍发生具有一定价值。
文摘AIM: To evaluate the association of complement factor H(CFH) and microtubule-associated protein 1 light chain 3 beta(MAP1LC3B) gene polymorphisms with the risk of age-related macular degeneration(AMD) in a high-altitude population. METHODS: The study group consisted of 172 participants with symptoms of AMD who were examined and diagnosed between January 2019 and June 2020. The control group was composed of 120 healthy individuals. Each participant was required to provide two milliliters of peripheral blood for DNA extraction. Two single nucleotide polymorphisms(SNPs) of CFH(rs1061170 and rs800292) and two SNPs of MAP1LC3B(rs8044820 and rs9903) were genotyped. The genotypes and allele frequencies of the SNPs in the study and control groups were further compared using Chi-square and Fisher’s exact tests. RESULTS: In a high-altitude population, the nominally significant differences of rs800292 and rs9903’s genotype AG frequencies were observed in the AMD group(P=0.034 and 0.004, respectively). The frequencies of allele G of rs800292 and allele A of rs9903 were also significantly dif ferent in the AMD group compared to the control [(P=0.034, OR=0.70, 95%CI: 0.50-0.98) and(P=0.004, OR=1.60, 95%CI: 1.15-2.22), respectively]. No significant differences in the genotype distributions(P=0.16 and 0.40, respectively) and allele frequencies(P>0.05) of rs1061170 and rs8044820 were observed in the AMD group.CONCLUSION: Genotype AG of rs800292 may be a protective factor for AMD. Conversely, rs9903 seems to be a risk factor for AMD. Therefore, allele G of rs800292 may be a protective factor, and allele A of rs9903, a risk factor for AMD in Qinghai high-altitude population.
