Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare pract...Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.展开更多
Cultured meat technology is a novel and promising strategy for sustainable and effective meat production.Muscle stem cells are widely used as seed cell populations because of their ease of access and myogenic differen...Cultured meat technology is a novel and promising strategy for sustainable and effective meat production.Muscle stem cells are widely used as seed cell populations because of their ease of access and myogenic differentiation potential.However,muscle stem cells are difficult to continuously propagate ex vivo and are heavily dependent upon serum for survival and growth,which impedes their commercial use as a cultured meat source.Herein,we identified an effective four-cytokine combination to promote long-term proliferation of porcine muscle stem cells using a serial elimination screening approach,which was consisted of long chain human insulin growth factor-1,platelet derived growth factor BB,basic fibroblast growth factor,and epidermal growth factor.The expansion of muscle stem cells with the four-cytokine combination achieved a 6.31×107-fold increase in cell number after 28 days of culture with retained cell myogenic differentiation potential,and most importantly,reduced the amount of fetal bovine serum required for cell culture by at least 5%.Furthermore,the four-cytokine combination exerted the pro-proliferative function by activating PI3K/Akt/mTOR and MEK/ERK signaling pathways.This approach provides for a new means by which to industrialize the production of cultured meat.展开更多
A key step in the manufacturing of cultured meat is to produce myotubes by induced myogenic differentiation.The development of effective,low-cost,and food-safe components that promote the in vitro differentiation of m...A key step in the manufacturing of cultured meat is to produce myotubes by induced myogenic differentiation.The development of effective,low-cost,and food-safe components that promote the in vitro differentiation of myoblasts is essential for the industrialization of cultured meat.Flavonoids are a class of plant secondary metabolites with various biological activities,but their effects on the regulation of myoblast behaviors are a lack of study.In this study,we selected four representative flavonoids including luteolin,chrysin,apigenin,and genistein,and investigated their effects on porcine myoblasts in the aspects of proliferation,migration,and differentiation.The results showed that four flavonoids all had relatively low cell toxicity but weak ability to promote the proliferation of porcine myoblasts.A positive effect of luteolin was observed in the migration and differentiation of porcine myoblasts,as indicated by improved migration rate and fusion index,as well as upregulated expression of Myogenin and MyHC.Pharmacological inhibition of PI3K activity attenuated the efficacy of luteolin on porcine myoblasts,and further analysis showed that luteolin increased the phosphorylation of PI3K,Akt,and mTOR,indicating the activation of the PI3K/Akt/mTOR signaling pathway.In conclusion,these findings showed that luteolin promoted the migration and differentiation of porcine myoblast via the activation of PI3K/Akt/mTOR signaling,providing biological evidence for its application in cultured meat production.展开更多
文摘Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.
基金This work was financially supported by the Innovative Research Groups Project of the National Natural Science Foundation of China(32021005)the National Key Research and Development Program of China(2021YFC2101400)+3 种基金the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SN-ZJU-SIAS-0013)the National Natural Science Foundation of China(3210160732)the Natural Science Foundation of Jiangsu Province(BK20210462)the Natural Science Foundation of Jiangsu Province(BK20202002).
文摘Cultured meat technology is a novel and promising strategy for sustainable and effective meat production.Muscle stem cells are widely used as seed cell populations because of their ease of access and myogenic differentiation potential.However,muscle stem cells are difficult to continuously propagate ex vivo and are heavily dependent upon serum for survival and growth,which impedes their commercial use as a cultured meat source.Herein,we identified an effective four-cytokine combination to promote long-term proliferation of porcine muscle stem cells using a serial elimination screening approach,which was consisted of long chain human insulin growth factor-1,platelet derived growth factor BB,basic fibroblast growth factor,and epidermal growth factor.The expansion of muscle stem cells with the four-cytokine combination achieved a 6.31×107-fold increase in cell number after 28 days of culture with retained cell myogenic differentiation potential,and most importantly,reduced the amount of fetal bovine serum required for cell culture by at least 5%.Furthermore,the four-cytokine combination exerted the pro-proliferative function by activating PI3K/Akt/mTOR and MEK/ERK signaling pathways.This approach provides for a new means by which to industrialize the production of cultured meat.
基金financially supported by the Innovative Research Groups Project of the National Natural Science Foundation of China(32021005)the National Key Research and Development Program of China(2021YFC2101400)+4 种基金the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SN-ZJU-SIAS-0013)the National Natural Science Foundation of China(3210160732)the Natural Science Foundation of Jiangsu Province(BK20210462)the Natural Science Foundation of Jiangsu Province(BK20202002)the Undergraduate Innovation Training Program of China(202110295007Z).
文摘A key step in the manufacturing of cultured meat is to produce myotubes by induced myogenic differentiation.The development of effective,low-cost,and food-safe components that promote the in vitro differentiation of myoblasts is essential for the industrialization of cultured meat.Flavonoids are a class of plant secondary metabolites with various biological activities,but their effects on the regulation of myoblast behaviors are a lack of study.In this study,we selected four representative flavonoids including luteolin,chrysin,apigenin,and genistein,and investigated their effects on porcine myoblasts in the aspects of proliferation,migration,and differentiation.The results showed that four flavonoids all had relatively low cell toxicity but weak ability to promote the proliferation of porcine myoblasts.A positive effect of luteolin was observed in the migration and differentiation of porcine myoblasts,as indicated by improved migration rate and fusion index,as well as upregulated expression of Myogenin and MyHC.Pharmacological inhibition of PI3K activity attenuated the efficacy of luteolin on porcine myoblasts,and further analysis showed that luteolin increased the phosphorylation of PI3K,Akt,and mTOR,indicating the activation of the PI3K/Akt/mTOR signaling pathway.In conclusion,these findings showed that luteolin promoted the migration and differentiation of porcine myoblast via the activation of PI3K/Akt/mTOR signaling,providing biological evidence for its application in cultured meat production.
